Microbial transformation of a triterpene glycoside, quinovic acid 3-O-β-D-quinovopyranoside by Streptomyces griseus ACCT 13273 was achieved with the aim of generating new bio-active derivatives. Preparative-scale bio...Microbial transformation of a triterpene glycoside, quinovic acid 3-O-β-D-quinovopyranoside by Streptomyces griseus ACCT 13273 was achieved with the aim of generating new bio-active derivatives. Preparative-scale biotransformation with standard two-stage fermentation protocol afforded a new oxidized metabolite, as a result of the hydroxylation of the methyl group at C-30, which was difficult to achieve by chemical means. The metabolite was elucidated based on extensive NMR and high-resolution mass spectral analyses.展开更多
文摘Microbial transformation of a triterpene glycoside, quinovic acid 3-O-β-D-quinovopyranoside by Streptomyces griseus ACCT 13273 was achieved with the aim of generating new bio-active derivatives. Preparative-scale biotransformation with standard two-stage fermentation protocol afforded a new oxidized metabolite, as a result of the hydroxylation of the methyl group at C-30, which was difficult to achieve by chemical means. The metabolite was elucidated based on extensive NMR and high-resolution mass spectral analyses.
