[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavo...[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.展开更多
近年来,在相关政策的加持下,我国农村电子商务取得了前所未有的发展。然而,受农业生产环境、技术条件、基础设施等限制,农村电子商务的发展仍然面临一些困境。加上经历了网络泡沫的寒冬,电子商务现已进入理性时代,缺乏创新的传统电商模...近年来,在相关政策的加持下,我国农村电子商务取得了前所未有的发展。然而,受农业生产环境、技术条件、基础设施等限制,农村电子商务的发展仍然面临一些困境。加上经历了网络泡沫的寒冬,电子商务现已进入理性时代,缺乏创新的传统电商模式已不能满足消费者需求,将面临被淘汰的境遇。因此,农村电子商务需要寻求新的商业模式和营销模式。F2O (Focus to online)是一种新兴的将焦点事件与电子商务结合的电商模式,对于解决农村电子商务发展困境具有启发性作用。基于此,本文结合4R营销理论从关联策略、反应策略、关系策略、回报策略探讨了F2O模式在农村电子商务中的应用。In recent years, with the support of relevant policies, rural e-commerce in our country has achieved unprecedented development. However, due to the limitations of agricultural production environment, technical conditions, and infrastructure, the development of rural e-commerce still faces some difficulties. Coupled with the cold winter of the Internet bubble, e-commerce has entered the rational era, and the traditional e-commerce model that lacks innovation can no longer meet the needs of consumers and will face the situation of being eliminated. Therefore, rural e-commerce needs to seek new business models and marketing models. F2O (Focus to online) is an emerging e-commerce model that combines focus events with e-commerce, which is enlightening to solve the dilemma of rural e-commerce development. Based on this, this paper discusses the application of F2O model in rural e-commerce from the relevancy strategy, reaction strategy, relation strategy and reward strategy combined with 4R marketing theory.展开更多
为了考察不同抽吸状态下卷烟动态通风特征,测量了3R4F卷烟在非燃烧不同抽吸状态下的压降与总通风率,建立了包含通风孔结构的全烟支气体流动3维数学模型,通过测量3R4F卷烟在17.5 m L/s恒速抽吸过程中的压降与总通风率对数学模型进行了验...为了考察不同抽吸状态下卷烟动态通风特征,测量了3R4F卷烟在非燃烧不同抽吸状态下的压降与总通风率,建立了包含通风孔结构的全烟支气体流动3维数学模型,通过测量3R4F卷烟在17.5 m L/s恒速抽吸过程中的压降与总通风率对数学模型进行了验证,发现测量值和模拟值两者相对误差均小于1.5%。利用所建数学模型分析了17.5 m L/s恒速抽吸状态下与ISO标准抽吸状态下3R4F卷烟内部气流压降和速率的3维分布规律以及轴向分布规律。结果显示:在恒速抽吸状态下,从3R4F卷烟纸进入卷烟内部的气流速率与距卷烟气流入口端距离成正比例关系;在ISO标准抽吸状态下,3R4F卷烟压降与抽吸流量呈正比例关系,而卷烟总通风率与抽吸流量无关并保持恒定不变。所得结果可为深入理解卷烟燃烧过程中气流运动规律、燃烧过程调控机理以及卷烟参数优化设计提供理论支持。展开更多
基金Supported by Youth Fund Project of Zhaoqing University(QZ202235)Zhaoqing Science and Technology Plan Project(2022040311011).
文摘[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.
文摘近年来,在相关政策的加持下,我国农村电子商务取得了前所未有的发展。然而,受农业生产环境、技术条件、基础设施等限制,农村电子商务的发展仍然面临一些困境。加上经历了网络泡沫的寒冬,电子商务现已进入理性时代,缺乏创新的传统电商模式已不能满足消费者需求,将面临被淘汰的境遇。因此,农村电子商务需要寻求新的商业模式和营销模式。F2O (Focus to online)是一种新兴的将焦点事件与电子商务结合的电商模式,对于解决农村电子商务发展困境具有启发性作用。基于此,本文结合4R营销理论从关联策略、反应策略、关系策略、回报策略探讨了F2O模式在农村电子商务中的应用。In recent years, with the support of relevant policies, rural e-commerce in our country has achieved unprecedented development. However, due to the limitations of agricultural production environment, technical conditions, and infrastructure, the development of rural e-commerce still faces some difficulties. Coupled with the cold winter of the Internet bubble, e-commerce has entered the rational era, and the traditional e-commerce model that lacks innovation can no longer meet the needs of consumers and will face the situation of being eliminated. Therefore, rural e-commerce needs to seek new business models and marketing models. F2O (Focus to online) is an emerging e-commerce model that combines focus events with e-commerce, which is enlightening to solve the dilemma of rural e-commerce development. Based on this, this paper discusses the application of F2O model in rural e-commerce from the relevancy strategy, reaction strategy, relation strategy and reward strategy combined with 4R marketing theory.
文摘为了考察不同抽吸状态下卷烟动态通风特征,测量了3R4F卷烟在非燃烧不同抽吸状态下的压降与总通风率,建立了包含通风孔结构的全烟支气体流动3维数学模型,通过测量3R4F卷烟在17.5 m L/s恒速抽吸过程中的压降与总通风率对数学模型进行了验证,发现测量值和模拟值两者相对误差均小于1.5%。利用所建数学模型分析了17.5 m L/s恒速抽吸状态下与ISO标准抽吸状态下3R4F卷烟内部气流压降和速率的3维分布规律以及轴向分布规律。结果显示:在恒速抽吸状态下,从3R4F卷烟纸进入卷烟内部的气流速率与距卷烟气流入口端距离成正比例关系;在ISO标准抽吸状态下,3R4F卷烟压降与抽吸流量呈正比例关系,而卷烟总通风率与抽吸流量无关并保持恒定不变。所得结果可为深入理解卷烟燃烧过程中气流运动规律、燃烧过程调控机理以及卷烟参数优化设计提供理论支持。
