The fruit flies of the Bactrocera （Diptera： Tephritidae） are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s r...The fruit flies of the Bactrocera （Diptera： Tephritidae） are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s rDNA sequences, the phylogenetic relationships among seven Bactrocera species, Bactrocera cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, B. caudata （Fabricius）, B. scutellata （Hendel）, B. dorsalis （Hendel）, and B. minax （Enderlein） were investigated. Nucleotide diversity within species ranged from 0.3% to 10.9%. According to the result, B. cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, and B. caudata （Fabricius） have no enough sites to be distinguished among the other species in the 347bp nucleotide sequences of the 16s rDNA gene. At the same time, excluding B. scutellata （Hendel） and B. minax （Enderlein）, the other species recognition sites are not only too little but also discontinuous. Phylogenetic trees calculated from both maximum parsimony and neighbor-joining phylogenetic analysis methods showed that B. cucurbitae and B. tau were closely related, B. diaphora （Hendel） and B. caudata （Fabricius） also had a close relationship. B. minax （Enderlein） has a furthest relationship from the other 6 species. From the results, we can infer that the our data supports previous classification of Bactrocera based on morphological characters in other words, but the Bactrocera subgenera, Zeugodacus is polyphyletic.展开更多
基金This research was funded bythe grants fromthe National Natural Science Foundation of China(No.30330090) ,the National Science FundforFostering Talents in Basic Research(No.NSFC-J0030092)and the Chinese Academy of Sciences Innovation Program(No.KSCX3-IOZ-01)
文摘The fruit flies of the Bactrocera （Diptera： Tephritidae） are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s rDNA sequences, the phylogenetic relationships among seven Bactrocera species, Bactrocera cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, B. caudata （Fabricius）, B. scutellata （Hendel）, B. dorsalis （Hendel）, and B. minax （Enderlein） were investigated. Nucleotide diversity within species ranged from 0.3% to 10.9%. According to the result, B. cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, and B. caudata （Fabricius） have no enough sites to be distinguished among the other species in the 347bp nucleotide sequences of the 16s rDNA gene. At the same time, excluding B. scutellata （Hendel） and B. minax （Enderlein）, the other species recognition sites are not only too little but also discontinuous. Phylogenetic trees calculated from both maximum parsimony and neighbor-joining phylogenetic analysis methods showed that B. cucurbitae and B. tau were closely related, B. diaphora （Hendel） and B. caudata （Fabricius） also had a close relationship. B. minax （Enderlein） has a furthest relationship from the other 6 species. From the results, we can infer that the our data supports previous classification of Bactrocera based on morphological characters in other words, but the Bactrocera subgenera, Zeugodacus is polyphyletic.
