Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gaine...Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gained the preference since reliable facilitative and inexpensive. In order to promote the DGGE further development and make it become a general analysis technique earlier, the key procedures and the advances in microbial communities analysis of DGGE were introduced in this paper.展开更多
The microbial molecular ecology techniques, which were developed on the basis of molecular, were applied in studying the bacteria in Constructed Rapid Infiltration （CRI） system. These techniques are very efficient i...The microbial molecular ecology techniques, which were developed on the basis of molecular, were applied in studying the bacteria in Constructed Rapid Infiltration （CRI） system. These techniques are very efficient in better describing the bacterial diversity, microbial community distribution, and relations between microbial group structure and nitrogen contamination through the analysis of microbial nucleic acid sequence fragment in CRI. The results further revealed the removal mechanism of contamination, which is essential for the improvement of wastewater treatment in CRI.展开更多
酸杆菌是土壤中一类重要的细菌类群。基于16S r RNA基因序列分析发现,酸杆菌一般占细菌总量的20%左右,甚至高达50%以上,表明酸杆菌在土壤生态过程中起到重要的作用。从植被、海拔高度、氮肥管理及二氧化碳升高对土壤酸杆菌分布的影响,...酸杆菌是土壤中一类重要的细菌类群。基于16S r RNA基因序列分析发现,酸杆菌一般占细菌总量的20%左右,甚至高达50%以上,表明酸杆菌在土壤生态过程中起到重要的作用。从植被、海拔高度、氮肥管理及二氧化碳升高对土壤酸杆菌分布的影响,以及酸杆菌根际效应等几方面论述了土壤酸杆菌门细菌生态学研究进展;综合分析揭示出不同分类单元酸杆菌细菌分布与环境因子间的关系;阐述了部分酸杆菌细菌的潜在生态功能。最后指出在土壤酸杆菌研究中应强化分离培养、细化分子生态、采用宏基因组学和单细胞测序新技术的重要性。展开更多
基金Supported by Program for New Century Excellent Talents in Ministry of Education(2005800612)
文摘Recently, denaturing gradient gel electrophoresis （DGGE） is well established molecular tool in microbial ecology that allows the study of diversity and dynamics of microbial communities. The technique has been gained the preference since reliable facilitative and inexpensive. In order to promote the DGGE further development and make it become a general analysis technique earlier, the key procedures and the advances in microbial communities analysis of DGGE were introduced in this paper.
基金supported by the National Key Project for Basic Research (No. 2007CB815600)the Project of the Ministry of Science and Technology of China (No. 2006BAD25B04)
文摘The microbial molecular ecology techniques, which were developed on the basis of molecular, were applied in studying the bacteria in Constructed Rapid Infiltration （CRI） system. These techniques are very efficient in better describing the bacterial diversity, microbial community distribution, and relations between microbial group structure and nitrogen contamination through the analysis of microbial nucleic acid sequence fragment in CRI. The results further revealed the removal mechanism of contamination, which is essential for the improvement of wastewater treatment in CRI.
文摘酸杆菌是土壤中一类重要的细菌类群。基于16S r RNA基因序列分析发现,酸杆菌一般占细菌总量的20%左右,甚至高达50%以上,表明酸杆菌在土壤生态过程中起到重要的作用。从植被、海拔高度、氮肥管理及二氧化碳升高对土壤酸杆菌分布的影响,以及酸杆菌根际效应等几方面论述了土壤酸杆菌门细菌生态学研究进展;综合分析揭示出不同分类单元酸杆菌细菌分布与环境因子间的关系;阐述了部分酸杆菌细菌的潜在生态功能。最后指出在土壤酸杆菌研究中应强化分离培养、细化分子生态、采用宏基因组学和单细胞测序新技术的重要性。
