Glycerol,dimethyl suffixod (DMSO) and acetamide were adopted to verify the cryoprotective ability of penetrating cryoprotectants with three kinds of extenders for rabbit sperm. Three extenders (A,B and C) and three le...Glycerol,dimethyl suffixod (DMSO) and acetamide were adopted to verify the cryoprotective ability of penetrating cryoprotectants with three kinds of extenders for rabbit sperm. Three extenders (A,B and C) and three level of the final concentrations of glycerol and DMSO (2%, 3% and 4% ),and acetamide 2%,4% and 5% were used with Extender A, 4% glycerol had better cryoprotective effect for rabbit sperm motility and 5% acetamide was better with Extender C. 4% acetamide was better in being combined with Extender A for sperm acrosome integrity ratio,5% acetamide was better with Extender B and 2% acetamide was better with Extende C. With extender A,there was not difference among three penetrating cryoprotectants for plasma membrane integrity. With Extender B, 3% glycerol was better than 4% acetamide for plasma membrane in- tegrity,and 3% glycerol with Extender C was better than 2% - 4% DMSO and 2% acetamide.展开更多
Sterols are essential components of the cell membrane lipid bilayer that include molecules such as cholesterol and desmosterol, which are significantly found in the spermatozoa of several animal species. However, the ...Sterols are essential components of the cell membrane lipid bilayer that include molecules such as cholesterol and desmosterol, which are significantly found in the spermatozoa of several animal species. However, the presence of desmosterol in rabbit semen has never been investigated. The aims of this study were to characterize the sterol composition of subfractions of ejaculated rabbit semen and evaluate the in vitro effects of sterol on the spermatozoa acrosome reaction and motility. Two sterols, occurring prevalently in the free form （94.3%）, were identified in whole semen collected from 10 fertile New Zealand White rabbits, specifically desmosterol （58.5% of total sterols） and cholesterol （35.9% of total sterols）. Desmosterol was the predominant sterol found in all subfractions of rabbit semen, varying from 56.7% （in the prostatic secretory granules, PSGs） to 63.8% （in the seminal plasma）. Spermatozoa contained an intermediate proportion of desmosterol （59.8%）, which was asymmetrically distributed between the heads （52.0% of the total content of sterols） and the tails （81.8%）. Results showed that both desmosterol and cholesterol can be transferred from the PSGs to the spermatozoa and are equally effective in inhibiting in vitro spermatozoa capacitation at a concentration higher than 1 mg L^-1. In contrast, neither desmosterol nor cholesterol had a significant effect on spermatozoa motility. Thus, it was concluded that, the various fractions of rabbit seminal fluid differ from each other in sterol composition and quantity, probably due to their different functional properties, and these fractions may undergo significant sterol changes depending on the stage of spermatozoa capacitation.展开更多
基金The project was supported by Heilongjiang youth Fund.
文摘Glycerol,dimethyl suffixod (DMSO) and acetamide were adopted to verify the cryoprotective ability of penetrating cryoprotectants with three kinds of extenders for rabbit sperm. Three extenders (A,B and C) and three level of the final concentrations of glycerol and DMSO (2%, 3% and 4% ),and acetamide 2%,4% and 5% were used with Extender A, 4% glycerol had better cryoprotective effect for rabbit sperm motility and 5% acetamide was better with Extender C. 4% acetamide was better in being combined with Extender A for sperm acrosome integrity ratio,5% acetamide was better with Extender B and 2% acetamide was better with Extende C. With extender A,there was not difference among three penetrating cryoprotectants for plasma membrane integrity. With Extender B, 3% glycerol was better than 4% acetamide for plasma membrane in- tegrity,and 3% glycerol with Extender C was better than 2% - 4% DMSO and 2% acetamide.
文摘Sterols are essential components of the cell membrane lipid bilayer that include molecules such as cholesterol and desmosterol, which are significantly found in the spermatozoa of several animal species. However, the presence of desmosterol in rabbit semen has never been investigated. The aims of this study were to characterize the sterol composition of subfractions of ejaculated rabbit semen and evaluate the in vitro effects of sterol on the spermatozoa acrosome reaction and motility. Two sterols, occurring prevalently in the free form （94.3%）, were identified in whole semen collected from 10 fertile New Zealand White rabbits, specifically desmosterol （58.5% of total sterols） and cholesterol （35.9% of total sterols）. Desmosterol was the predominant sterol found in all subfractions of rabbit semen, varying from 56.7% （in the prostatic secretory granules, PSGs） to 63.8% （in the seminal plasma）. Spermatozoa contained an intermediate proportion of desmosterol （59.8%）, which was asymmetrically distributed between the heads （52.0% of the total content of sterols） and the tails （81.8%）. Results showed that both desmosterol and cholesterol can be transferred from the PSGs to the spermatozoa and are equally effective in inhibiting in vitro spermatozoa capacitation at a concentration higher than 1 mg L^-1. In contrast, neither desmosterol nor cholesterol had a significant effect on spermatozoa motility. Thus, it was concluded that, the various fractions of rabbit seminal fluid differ from each other in sterol composition and quantity, probably due to their different functional properties, and these fractions may undergo significant sterol changes depending on the stage of spermatozoa capacitation.
