Objective: To research the relationship among CEA expression in tumor, CEA serum level and Radioimmunoguided Surgery (RIGS) in Colorectal Cancer. Methods: twenty-nine patients with colorectal cancer wer administered r...Objective: To research the relationship among CEA expression in tumor, CEA serum level and Radioimmunoguided Surgery (RIGS) in Colorectal Cancer. Methods: twenty-nine patients with colorectal cancer wer administered radiolabeled McAb CL58 submucosally via endoscope, and all underwent surgical intervention 3 to 14 days later. Intraoperative radioimmunodetection was performed using a portable gamma-detecting probe (GDP). The counts of tumors were obtained, and tumor-to-normal tissue (T/NT) ratio was calculated. The T/NT ratio of 3:1 was taken as the lowest threshold value of positiveness. Anti-CEA immunohistochemical for all the samples were performed. The CEA serum levels of all the patients before operations were also recorded. Results: The sensitivity for RIGS in detecting primary lesions was 93.1% (27/29). 28 patients (96.6%) had CEA expressions to a greater or less extent. 5 patients (17.2%) had elevated CEA serum. There was no correlation between the CEA serum levels and results of RIGS (P>0.5), and no correlation between the CEA serum levels and CEA expression in tumor (P>0.5). The CEA expression in tumor correlated significantly with the RIGS results (P<0.05). Conclusion: This study indicates that immunostaining can be used to select the patients as candidates who will benefit most from RIGS.展开更多
Objectival. To prepare a radioimmunoguided surgery （RIGS） system including radio-nuclides hand- held gamma detecting probe （GDP） with signal processing control unit, specific monoclonal antibody （MAb）, technique...Objectival. To prepare a radioimmunoguided surgery （RIGS） system including radio-nuclides hand- held gamma detecting probe （GDP） with signal processing control unit, specific monoclonal antibody （MAb）, technique of labeling as well as detection of ovarian carcinoma in nude mice bearing human ovari-an carcinoma. Metals. MAb COCl83B2, produced in our laboratory was used to prepare the F （ah’）2, fragment by ficin digestion. 99mTc labeling of COC183 B2-F（ab’）2, and normal mice IgG were prepared according to im- proved pretining method. GDP and control unit were made by Chinese Atomic Energy Research Institute. Different groups of nude mice bearing human ovarian carcinoma with ascites were administered with differ- ent dosages of 99mTo COCl83 B2-F（ab’）2, and their tissues measured by GDP in vivo and compared with gamma well counts after dissection in vitro. Results. 99mTc labeled COCl83B2-F（ab’）2, yielded a labeling rate of over 90%, and a good immunoac- tivity. The detections of carcinoma tissue by GDP showed a T/NT ratio of 2: 1 as positive for cancer. The smallest carcinoma detected was 0. 2cm × 0.1 cm and the nearest distance between carcinoma and normal tissue was 0. 2cm. Conclusions. The above studies proved that ficin can be used to digest MAbs with high yields and good immunoactivity of F（ab’）2. Our RIGS system with 99mTo labeled COC183 B2-F（ab’）2 and GDP can hope-fully be used clinically for ovarian carcinoma detection.展开更多
基金This work was supported by the State 863 High Technology R&D Project of China (No.2001AA21537).
文摘Objective: To research the relationship among CEA expression in tumor, CEA serum level and Radioimmunoguided Surgery (RIGS) in Colorectal Cancer. Methods: twenty-nine patients with colorectal cancer wer administered radiolabeled McAb CL58 submucosally via endoscope, and all underwent surgical intervention 3 to 14 days later. Intraoperative radioimmunodetection was performed using a portable gamma-detecting probe (GDP). The counts of tumors were obtained, and tumor-to-normal tissue (T/NT) ratio was calculated. The T/NT ratio of 3:1 was taken as the lowest threshold value of positiveness. Anti-CEA immunohistochemical for all the samples were performed. The CEA serum levels of all the patients before operations were also recorded. Results: The sensitivity for RIGS in detecting primary lesions was 93.1% (27/29). 28 patients (96.6%) had CEA expressions to a greater or less extent. 5 patients (17.2%) had elevated CEA serum. There was no correlation between the CEA serum levels and results of RIGS (P>0.5), and no correlation between the CEA serum levels and CEA expression in tumor (P>0.5). The CEA expression in tumor correlated significantly with the RIGS results (P<0.05). Conclusion: This study indicates that immunostaining can be used to select the patients as candidates who will benefit most from RIGS.
文摘Objectival. To prepare a radioimmunoguided surgery （RIGS） system including radio-nuclides hand- held gamma detecting probe （GDP） with signal processing control unit, specific monoclonal antibody （MAb）, technique of labeling as well as detection of ovarian carcinoma in nude mice bearing human ovari-an carcinoma. Metals. MAb COCl83B2, produced in our laboratory was used to prepare the F （ah’）2, fragment by ficin digestion. 99mTc labeling of COC183 B2-F（ab’）2, and normal mice IgG were prepared according to im- proved pretining method. GDP and control unit were made by Chinese Atomic Energy Research Institute. Different groups of nude mice bearing human ovarian carcinoma with ascites were administered with differ- ent dosages of 99mTo COCl83 B2-F（ab’）2, and their tissues measured by GDP in vivo and compared with gamma well counts after dissection in vitro. Results. 99mTc labeled COCl83B2-F（ab’）2, yielded a labeling rate of over 90%, and a good immunoac- tivity. The detections of carcinoma tissue by GDP showed a T/NT ratio of 2: 1 as positive for cancer. The smallest carcinoma detected was 0. 2cm × 0.1 cm and the nearest distance between carcinoma and normal tissue was 0. 2cm. Conclusions. The above studies proved that ficin can be used to digest MAbs with high yields and good immunoactivity of F（ab’）2. Our RIGS system with 99mTo labeled COC183 B2-F（ab’）2 and GDP can hope-fully be used clinically for ovarian carcinoma detection.
