Effects of Huangzhou Radish Powder on the Quality of Silver Carp Surimi Products

[Objectives]This study was conducted to improve the quality of frozen silver carp surimi products.[Methods]Huangzhou radish powder and silver carp surimi were used as raw materials to analyze the effects of different amounts(1%,2%,3%,4%,5%)of Huangzhou radish powder on the water-holding capacity,cooking loss rate,whiteness value,TPA,gel strength and sensory quality of surimi products.[Results]When the addition amount of Huangzhou radish powder was 1%,water-holding capacity,hardness,chewiness and the gel strength of the surimi product reached their maximum values,which were 86.86%,3470.36 g,2628.50 g and 707.48 g·mm,respectively.Meanwhile,the cooking loss rate was reduced to a minimum value(13.66%),and the sensory quality reached the best.The whiteness of surimi products showed a decreasing trend with the increase of the addition amount of Huangzhou radish powder.However,the whiteness of the surimi product was not significantly affected when the addition amount of Huangzhou radish powder was 1%.Therefore,when the addition amount of Huangzhou radish powder was 1%,the quality of surimi products could be improved.[Conclusions]This study provides a new idea for the development and utilization of Huangzhou radish.

Effect of Brassinosteroids on Germination and Seedling Growth of Radish (<i>Raphanus sativus</i>L.) under PEG-6000 Induced Water Stress

Effect of 24-epibrassinolide and 28-homobrassinolide on the germination and seedling growth of radish (Raphanus sativus) subjected to water stress being imposed by 15% (w/v) polyethylene glycol (equivalent to -2.95 bars of osmotic pressure) was studied. Brassinosteroids supplementation reduced the inhibitory effect of water stress on seed germination and seedling growth. The growth stimulation in radish seedlings by brassinosteroids under desiccation stress was associated with elevated levels of nucleic acids and soluble proteins and lowered activities of RNase. The mitigation of osmotic stress imposed by PEG was associated with increased scavenging of reactive oxygen species as reflected in elevated activities of superoxide dismutase, catalase and ascorbate peroxidase. Brassinosteroids also enhanced the accumulation of the osmolyte free proline in radish seedlings challenged with drought stress. The two brassinosteroids also maintained membrane integrity under water deficiency conditions as indicated by lowered lipid peroxidation reflecting in reduced MDA content. The results of present study demonstrate the protective role of brassinosteroids against PEG imposed water stress in radish seedlings.

Osmoprotectants and Antioxidative Enzymes as Screening Tools for Salinity Tolerance in Radish (Raphanus sativus)

The research addresses the identification of a screening methodology for salt stress tolerance in radish cultivars. In the first experiment, two different radish cultivars(long white and round red) were compared in their morphological and physiological responses to different salinity levels. Round red radish showed better morphological and physiological responses to incremental salinity in terms of yield and better adaptation of overall water relations. In the second experiment, the most tolerant genotype from the first experiment was used as a control against other seven round red radish genotypes ranked by their salinity tolerance according to morphological, physiological and biochemical indices. Salt stress did not significantly affect malondialdehyde(MDA) and hydrogen peroxide(H2O2) content, and ascorbate peroxidase(APX) activity in the studied cultivars. Nonetheless, the relatively salt tolerant cultivar SAXA2 showed higher ability to accumulate compatible solutes(e.g.proline and proteins) and maintain osmotic adjustment. In addition, cultivar SAXA2 also showed considerable increase in glutathione reductase(GR) activity. Our results supported that accumulation of proline and higher GR activity are associated with radish salt tolerance, whereas no relationship with salinity was observed in superoxide dismutase(SOD), MDA and H2O2 content.

Development of Specific SSR Molecular Marker of "Huangzhou Radish" Based on Purity Identification

As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined deeply,so it is urgent to improve and strengthen the purity and viability of "Huangzhou radish" including bad phenomena such as degeneration of varieties after self-pollination.At present,there was no SSR molecular marker can be used for genetic diversity identification of "Huangzhou radish".SSR molecular marker can provide a favorable tool to study "Huangzhou radish".In this study,"Xiakang","Hongbao"and "Huangzhou radish" were used as experimental materials,100 SSR primers were screened,and 25 pairs of SSR primers were selected.Finally,two pairs of primers were selected for identification of the purity of "Huangzhou radish" by non-denatured polyacrylamide gel electrophoresis (PAGE).The results showed that R-102 and R-112 primer pairs could amplify the special bands for "Huangzhou radish" from above three kinds of radishes.These two pairs of primers could be developed as SSR markers to identify the specificity and purity of "Huangzhou radish".Furthermore,this study provides convenient conditions for promoting the economic and social value of "Huangzhou radish".

Genome-wide identification and expression profiling of MYB transcription factor genes in radish(Raphanus sativus L.)

Radish(Raphanus sativus L.), an important root vegetable crop of the Brassicaceae family, has a high level of anthocyanin accumulation in its pigment root tissues. It was reported that MYB transcription factors(TFs) play vital roles in plant development and anthocyanin metabolism, and the PAP1/2 could promote expression of anthocyanin biosynthesis genes. In this study, a total of 187 radish MYB genes(Rs MYBs) were identified in the radish genome and clustered into 32 subfamilies. Among them, 159 Rs MYBs were localized on nine radish chromosomes. Interestingly, 14 Rs MYBs exhibited differential expression profiles in different taproot developmental stages among four differently colored radish lines. A number of Rs MYBs were highly expressed in the pigmented root tissues at the maturity stage, several genes including Rs MYB41, Rs MYB117, and Rs MYB132 being homologous to PAP1/2, showed high expression levels in the red skin of NAU-YH(red skin-white flesh) taproot, while Rs MYB65 and Rs MYB159 were highly expressed in the purple root skin of NAU-YZH(purple skin-red flesh), indicating that these Rs MYBs might positively regulate the process of anthocyanin accumulation in radish taproot. These results would provide valuable information for further functional characterization of Rs MYBs, and facilitate clarifying the molecular mechanism underlying anthocyanin biosynthesis in radish.

Evaluation of Different Radish (Raphanus sativus) Genotypes under Different Saline Regimes

An effective and simple screening technique for identification of salt tolerant and salt sensitive radish genotypes was observed. Sand is used as potting media. Six genotypes of radish were used for screening against four salinity levels (0, 1, 3, 5 and 7 dS/m-1). Twenty days old seedlings of radish were salinized with salt solution (NaCl). Morphological, physiological and ionic parameters were studied. Radish genotypes Laal-Pari and 40 Days executed the best performance in all the measured attributes and categorized as salt tolerant genotype while Green Neck was the poorest in retaining normal functioning at higher salinity levels thus grouped under salt sensitive cultivar.

Study on Nutrients and Texture of Pickled Huangzhou Radish

[Objectives]The changes in the nutrient contents and texture of pickled Huangzhou radish with pickling time were studied to further improve its edible value and improve its sensory evaluation.[Methods]Huangzhou radish as a raw material was pickled,and exploration was conducted through the following aspects:with the extension time of pickling time,the changes in nutrient contents such as reducing sugars,free amino acids and ascorbic acid were detected in pickled Huangzhou radish,and the changes in the texture characteristics of pickled Huangzhou radish such as hardness,viscosity,elasticity,recoverable deformation,compression power and chewiness were detected.[Results]As the pickling time became longer,the reducing sugar content in pickled Huangzhou radish gradually increased,and when the pickling time exceeded 13 d,the reducing sugar content showed a downward trend;the ascorbic acid content gradually decreased,and the decreasing rate was relatively lower after the pickling time exceeded 7 d;and the amino acid content first increased and then decreased,and when the pickling time exceeded 13 d,the content exhibited basically no change.The hardness of the radish gradually decreased;the viscosity first decreased and then slowly increased;the recoverable deformation first increased and then decreased;the elasticity first increased and decreased;and the chewiness first increased and then decreased.Through this experiment,it is concluded that the pickled Huangzhou radish has richer nutrients and the best taste when the pickling time is 7-10 d.[Conclusions]This study provides a theoretical reference for producing and processing pickled Huangzhou radish,improving the quality of pickled Huangzhou radish and increasing the nutritional value of the product.

Identification and Control of Radish Alternaria Leaf Spot, Black Rot, Soft Rot, Virus Disease and Hollowness

The damage characteristics and occurrence regularity of radish alternaria leaf spot, black rot, soft rot, virus disease and hollowness are introduced in the paper, and the corresponding control method is proposed from two aspects of agricultural control and chemical control.

The Nutrient Expert decision support system improves nutrient use efficiency and environmental performance of radish in North China

Excessive fertilization has led to nutrient use inefficiency and serious environmental consequences for radish cultivation in North China.The Nutrient Expert(NE)system is a science-based,site-specific fertilization decision support system,but the updated NE system for radish has rarely been evaluated.This study aims to validate the feasibility of NE for radish fertilization management from agronomic,economic,and environmental perspectives.A total of 46 field experiments were conducted over four seasons from April 2018 to November 2019 across the major radish growing regions in North China.The results indicated that NE significantly reduced N,P_(2)O_(5),and K_(2)O application rates by 98,110,and 47 kg ha^(-1) relative to those in the farmers’practice(FP),respectively,and reduced N and P_(2)O_(5) inputs by 48 and 44 kg ha^(-1),respectively,while maintaining the same K_(2)O rate as soil testing(ST).Relative to FP and ST,NE significantly increased radish yield by 2.7 and 2.6 t ha^(-1)(4.2 and 4.0%)and net returns by 837 and 432 USD ha^(-1),respectively.On average,NE significantly improved the agronomic efficiency(AE)of N,P,and K(relative to FP and ST)by 42.4 and 31.0,67.4 kg kg^(-1) and 50.9,and 20.3 and 12.3 kg kg^(-1);enhanced the recovery efficiency(RE)of N,P,and K by 11.4 and 7.0,14.1 and 7.5,and 11.3 and 6.3 percentage points;and increased the partial factor productivity(PFP)of N,P,and K by 162.9 and 96.8,488.0 and 327.3,and 86.9 and 22.4 kg kg^(-1),respectively.Furthermore,NE substantially reduced N and P_(2)O_(5) surpluses by 105.1 and 115.1 kg ha^(-1),respectively,and decreased apparent N loss by 110.8 kg ha^(-1) compared to FP.These results indicated that the NE system is an effective and feasible approach for improving NUE and promoting cleaner radish production in North China.

Effect of plasma treatment on the seed germination and seedling growth of radish(Raphanus sativus)

The effect on the germination and seedling growth of radish(Raphanus sativus)seeds were examined employing a dielectric barrier discharge(DBD)at atmospheric pressure and room temperature for various treatment time.DBD plasma using argon gas of flow rate 21 m^(-1)was employed in this study.Radish seeds were treated with DBD plasma for 1-5 min,respectively.Germination characteristics,seedling growth parameters,the contact angle of the seed coat,water uptake capacity,mass loss,the temperature of the seeds,chlorophyll,and carotenoid contents of the seedlings were measured before and after the DBD plasma treatments.Plasma treatment of radish seeds significantly increased germination-related characters,including germination percentage,fresh and dry weight,vigor index,and total carotenoids contents.However,the cumulative production rate was found to be decreased.Results from the experiment indicate an acceleration in the water uptake of the radish seeds and make the seed surface hydrophilic by plasma treatment.Scanning electron microscopy analysis showed that etching effects on the seed coat occurred after the argon plasma treatments,which affected the wettability of the radish seed.The experimental findings showed that seeds being treated by DBD plasma for 2 and 3 min had a positive effect on the germination and seedling growth of radish.

Identification and Analysis of Components of Glucosinolates in Succulent Roots and Leaves of Fruity Radish (Raphanus sativas L.)

The components of glucosinolates( GSL) in the succulent roots and leaves of three kinds of fruity radish( Xinlimei,Super Zhengyan,and Shawo) were identified and analyzed by high performance liquid chromatography( HPLC). Results showed that there were nine kinds of glucosinolates in the succulent roots and leaves of three kinds of fruity radish,including six kinds of aliphatic glucosinolates( 4-methyl sulfinyl-3-butenyl glucosinolates,2-allyl glucosinolates,4-methyl sulfinyl butyl glucosinolates,5-methyl sulfinyl amyl glucosinolates,4-methyl n-butyl glucosinolates,4-methyl thio-3-butenyl glucosinolates) and three kinds of indole glucosinolates( 4-methoxy methyl indole glucosinolates,3-methyl indole glucosinolates,1-methoxy methyl indole glucosinolates),but there were significant differences in the content of glucosinolates between different varieties and organs. The total content of glucosinolates in succulent roots of three kinds of fruity radish was22 472. 84,13 585. 86,and 28 200. 70 μg/g DM respectively,and more than 95% were aliphatic glucosinolates. The content of glucosinolates in succulent roots was 4. 56,2. 71,and 4. 55 times of that in leaves of three kinds of fruity radish. Main aliphatic glucosinolates in succulent roots and leaves of three kinds of fruity radish were 4-methyl sulfinyl-3-butenyl glucosinolates,accounting for 90. 11%-93. 92% and63. 03%-73. 72% of total content of glucosinolates.

The Main Evaluation Indicators for the Quality of Fruit Radish

The aim of this study was to establish a scientific evaluation system for fruit radish quality. 8 quality indicators were measured for23 varieties,such as Meinong,Qiaotouqing and Chunbulao. The indicators included root weight,root shape index,water content,crispness,soluble solids,crude fiber,isothiocyanate and vitamin C. These data were analyzed by variation correlation analysis,principal component analysis and system cluster analysis. 23 varieties of fruit radish were analyzed by variation correlation; 8 indicators were compressed into 6 integrated variables using principal component analysis. Finally,the main evaluation indicators of fruit radish were determined by using cluster analysis and correlation analysis. The results indicated that the range of variation coefficient was from 1. 64%-89. 99%. Water content,crispness,soluble solids,crude fiber,isothiocyanate were the important components of fruit radish quality,playing a major role in the quality. These 8indicators were closely related and relatively independent. Water content was significantly and positively correlated with crispness; soluble solids and crude fiber were significantly and negatively related to vitamin C; crispness was significantly and positively correlated with crude fiber and vitamin C. Based on the above results,4 representative indicators were used for evaluating fruit radish quality,that is water content,crispness,soluble solids,crude fiber,which could reflect the most information of fruit radish quality.

Lead Absorption by Radish Is Affected by Soil Texture and Cultivar

Lead (Pb) was detected in potential urban garden soil in Claremont Cali-fornia and was absorbed by radish cultivars and giant red mustard in a bioassay of the contaminated soil. Radish cultivars grown in two soils with two lead salts developed the same lead tissue concentrations. In a subsequent study, Cultivar “Rudolf” accumulated less lead nitrate than cultivars “French Breakfast” and “White Beauty”. Cultivar “Rudolf” grew the least biomass likely accounting for its reduced Pb accumulation. All radish cultivars accumulated more lead when grown in clay vs. sand soil and radish Pb accumulation increased when the concentration of lead was increased in either soil type. The solubility of the lead ion used was not associated with Pb uptake by radish.

Comparative Effect of City Finished Compost and NPK Fertilizer on Growth and Availability of Phosphorus to Radish (<i>Raphanus sativus</i>L.)

A pot experiment was carried out to investigate the comparative effect of city finished compost and NPK fertilizer on the growth and availability of phosphorus to radish (Raphanus sativus L.). An air dried sandy loam soil was mixed with five rates of city finished compost (CFC) equivalent to 0, 5, 10, 20, 40 ton ha–1 and three rates of NPK fertilizer equivalent to 50% (N-P-K = 69-16-35 kg ha–1), 100% (N-P-K = 137-32-70 kg ha–1 K) and 150% (N-P-K = 206-48-105 kg ha–1). Four plants were harvested at 45 days of growth and remaining one plant was harvested at 90 days of growth and separated into leaves and bulbs. After harvest, soil samples were collected from each pot to measure soil pH and available P extracted by Olsen, Mehlich-3, Kelowna and Bray & Kurtz-1 extractants. The growth parameters (length of leaves and bulbs, fresh and dry weight of leaves and bulbs), relative dry matter yield, plant P concentrations, P uptake by radish, soil pH, and available P increased by the rates of CFC and NPK fertilizer treatments. Among the treatments, growth performance of radish was better with the highest rate of 40 t ha–1 CFC treatments. The results obtained from the 5 and 10 t ha–1 CFC treatment were comparable with the results of 50% and 100% NPK fertilizer treatments respectively. Similar effects of amendments were obtained in the case of plant P concentration, uptake of P by plant, soil pH and available soil P concentration. Available P and soil pH showed very strong and positive correlation (P –1 city finished compost could be used instead of 100% to obtain similar yield and to improve soil conditions.

Effect of Reducing Application of Chemical Fertilizers and Chemical Pesticides on Physiological Traits,Yield and Quality of Radish

This paper studied the effect of reducing application of chemical fertilizer,increasing application of functional organic fertilizer and trace elements and fully using biological pesticides on physiological traits,yield and quality of radish. The results showed that applying functional organic fertilizer and trace elements and fully using biological pesticides could help to significantly improve the radish fleshy root yield,increase soluble sugar and vitamin C content,reduce crude fiber and nitrate content,but had no significant influence on radish moisture content,glucosinolates and chlorpyrifos content in organophosphorus pesticides.

Effects of Low Temperature Treatment on Bolting of Radish(Raphanus sativus L.)

[Objectives]This study was conducted to screen out a vernalization method of radish that is more suitable for breeding and hybrid production.[Methods]Eight radish inbred lines with different bolting tolerance were used as the research objects for the experiment.The following three treatments were done:(1)treating at 4℃in the dark with heat preservation for 1,2,3,4,and 5 weeks,respectively~([1]),(2)treating at 4℃in the dark with heat preservation for 3 weeks after 24 h incubation in light at 20℃,and(3)treating under light at a low temperature of 6.5℃for three weeks after cultivating under light at 20℃to 4 true leaves.The effects of low temperature treatment on the bolting and flowering time of different varieties of radish treated at different developmental stages were analyzed.[Results]When comparing these three methods,the low-temperature treatment of wet seeds had a better effect on early bolting of radish and is simpler and more feasible.The effect of treating four-leaf seedlings at 6.5℃was the worst;most radish inbred lines could shorten bolting time when treated at 4℃for one week;and within a certain range,the longer the 4℃treatment time,the better the vernalization effect.Different radish inbred lines had different performances to low temperature treatment.[Conclusions]This study provides a scientific basis for the theoretical research on vernalization of radish.

Comparison of Spring Radish Varieties with Entire Leaves

[Objectives]This study was conducted to select spring radish varieties with better growth,higher yield and better nutrition.[Methods]A new spring radish combination(2019MUA×Lixiang)was obtained through traditional hybridization methods,and various characters of this line were compared with its parents and commercial spring radish.The radish varieties such as Lixiang radish,2019MUA×Lixiang,2019MUA,commercial variety Zhongluo No.1 and Huangzhou radish were respectively compared and studied in detail in terms of botanical characters,entire leaf bolting ratios,nutritional physiological indexes and so on.[Results]The new radish combination 2019MUA×Lixiang has better indexes,and is more suitable for spring planting.[Conclusions]The study provides a reference basis for the selection on radish planted in Spring.

Breeding of a New Fruit Radish Variety"Sheng Qing Yi Hao"

"Sheng Qing Yi Hao",a new fruit radish F1 hybrid,which was bred from HSR54 A,a self-bred sterile line,and HS1356,an excellent male parent.The flesh of the roots is emerald green,delicate,sweet and crisp.The skin of the roots is little spicy.It takes about 70 d from sowing to maturity.The fleshy roots have a length of 22-27 cm,a diameter of 6-8 cm,and a root weight of 0.8-1.1 kg.The F1 hybrid has stronger resistance to soft rot disease in the field the control group‘Chengyangqing’.It is suitable for cultivation in greenhouse in spring and open field in autumn in north China.

An Ideal Raw-radish Therapy for Allergic Rhinitis

[Objectives] To explore a kind of simple and effective dietotherapy for allergic rhinitis( AR). [Methods]48 patients with AR were hospitalized and randomly assigned to raw-radish treatment group( 36 patients) or placebo group( 12 patients). Every patient took about 150 g raw radish( experimental group) or cooked radish( placebo group) one time or separately a day. [Results]By continuously taking raw radish for fifteen days,the total cure rate reached 80. 6% and total valid rate reached 94. 4%,with the valid rate 100% for persistent AR and 66. 7% for intermittent AR. But cooked radish( placebo) was invalid to AR. Presumably the wonder efficacy of raw radish may be related with isothiocyanates in radish. Since isothiocyanates are susceptible to hydrolysis by cooking,so radish must be eaten raw. [Conclusions] Raw radish therapy works wonders for AR,which may be more effective,safer,cheaper and simpler method for prevention and treatment of AR at present.

Analysis and Evaluation of Nutritional Quality in Chinese Radish (Raphanus sativus L.)

Radish (Raphanus sativus L.) is an important vegetable crop worldwide. High nutritional quality was critical in itsgenetic improvement and production. The nutritional quality of 42 Chinese radish cultivars was analyzed in this study.The contents of six nutritional facts, dry matter (DM), crude fiber (CF), total soluble sugar (TSS), vitamin C (VC), protein,and nitrate, ranged from 29.7 to 88.2, 4.507 to 18.546, 2.233 to 15.457, 0.1416 to 0.3341, 0.34 to 1.15, and 1.81 to 5.89 g kg-1 freshweight (FW), respectively. Significant differences among the 42 radish cultivars were detected in the contents of nutritionalfacts. The data were subjected to cross-correlation analysis and principal component analysis (PCA). It was found that DMcontent was positively correlated with the content of TSS (r = 0.7104), VC (r = 0.4011) and protein (r = 0.4120). Vitamin C (VC)content of radish showed a positive correlation (r = 0.3300) with the protein content. According to the principal componentanalysis, out of the 42 radish cultivars, Nau-17, Nau-28, Nau-6, Nau-11, Nau-10, Nau-27, and Nau-31 were detected with veryhigh scores in comprehensive evaluation. It could be concluded that abundant diversity of nutritional fact content occurredin different radish genotypes, and PCA analysis was effective for selecting radish germplasm with high quality. The resultscould contribute useful knowledge of nutritional quality, and provide important germplasms for the elite cultivar developmentand the inheritance study of nutritional facts in radish.