目的探讨晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)基因的5个SNP位点在2型糖尿病伴慢性牙周炎、单纯慢性牙周炎、健康对照北方汉族人群中分布的差异,从而研究RAGE基因是否为糖尿病和牙周炎可能的致病...目的探讨晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)基因的5个SNP位点在2型糖尿病伴慢性牙周炎、单纯慢性牙周炎、健康对照北方汉族人群中分布的差异,从而研究RAGE基因是否为糖尿病和牙周炎可能的致病易感基因。方法运用飞行时间质谱法检测19例2型糖尿病伴慢性牙周炎患者(DM组),22例单纯慢性牙周炎组(CP组)以及54例健康对照组(H组)全血基因组DNA中5个单核苷酸多态性(single nucleotide polymorphism,SNP)位点。结果rs17846808、rs1800625、rs184003、rs2070600、rs3134940基因多态性在三组间分布无差异。DM组中的rs3134940 A/A(野生纯合子)基因型人群的龈沟出血指数(sulcus bleeding index,SBI)显著高于(A/G+G/G,杂合子和突变纯合子)基因型人群的SBI。CP组中的rs1800625(T/C+C/C)和rs3134940(A/G+G/G,杂合子和突变纯合子)基因型人群缺失牙数显著性增高。结论RAGE基因并非2型糖尿病以及慢性牙周炎的易感基因。rs3134940 G等位基因可能在2型糖尿病伴慢性牙周炎进展过程中起到一定保护作用。展开更多
Diabetes Mellitus is a chronic disease that affects important body organs in a very serious manner. The consequences of this disease turn out to be a significant problem for the patient, who tries to cope with the new...Diabetes Mellitus is a chronic disease that affects important body organs in a very serious manner. The consequences of this disease turn out to be a significant problem for the patient, who tries to cope with the new condition his organism has been placed in. The most common effect of the disease, hyperglycaemia, leads over time to serious damage to various body systems, such as nerves and blood vessels. What is not widely known among the population is that diabetes may have harmful effects on the reproductive system of the men suffering from diabetes type 1 and 2 and that such a parameter could lead to or might be the reason for infertility problems for couples, for example, miscarriage or embryonic failure. AGEs is a number of products which are believed to play an important role, because their presence has been detected in increased level in diabetic men. This implies that those glycation products might play a key role in diabetic complications. Their receptor, RAGE, member of the immunoglobulin superfamily has been detected in the reproductive tract of diabetic men. Reactive oxygen species (ROS), a possible product of AGEs appear in high levels in seminal plasma and are believed to be the cause of DNA fragmentation. The objective of this review was to gather all the available material i.e. studies on diabetes mellitus in one article, to study the research which has already been conducted and the conclusions that have been drawn, in order to offer, if possible, new pathways and perspectives to the scientists, who focus on fertility problems, sometimes intractable.展开更多
目的探讨晚期糖基化终产物受体(receptor of advanced glycation end products,RAGE)基因多态性与冠状动脉粥样硬化性心脏病(简称冠心病)患者单核细胞/高密度脂蛋白胆固醇比值(monocyte-to-HDL cholesterol ratio,MHR)和心率变异的相关...目的探讨晚期糖基化终产物受体(receptor of advanced glycation end products,RAGE)基因多态性与冠状动脉粥样硬化性心脏病(简称冠心病)患者单核细胞/高密度脂蛋白胆固醇比值(monocyte-to-HDL cholesterol ratio,MHR)和心率变异的相关性。方法以120名冠心病患者作为观察组,另选取同期健康体检人群120名作为对照组,分析两组以及不同严重程度患者的-429T>C、1704G>T、82G>S基因型及等位基因、MHR比值、心率变异性之间的差异,分析上述基因型与MHR比值和心率变异性的相关性。结果两组以及不同严重程度患者的RAGE基因82G>S多态性以及等位基因之间的差异有统计学意义(P<0.05)。与对照组和轻中度患者相比,观察组以及重度患者的单核细胞、总胆固醇、甘油三酯、低密度脂蛋白、MHR、低频功率显著偏高,高密度脂蛋白、NN间期标准差、RR间期平均值标准差、相邻RR间期差值、相邻RR间期差值>50 ms的心搏数占比、高频功率显著偏低。G-Gly-T、T-Gly-T、G-Ser-T、G-Gly-C的基因频率与NN间期标准差、RR间期平均值标准差、相邻RR间期差值、相邻RR间期差值>50 ms的心搏数占比、高频功率、MHR呈正相关,与低频功率呈负相关。结论冠心病患者的RAGE基因多态性与冠心病患者MHR比值和心率变异性的显著相关,可作为疾病诊断以及疗效评价的重要依据。展开更多
文摘Diabetes Mellitus is a chronic disease that affects important body organs in a very serious manner. The consequences of this disease turn out to be a significant problem for the patient, who tries to cope with the new condition his organism has been placed in. The most common effect of the disease, hyperglycaemia, leads over time to serious damage to various body systems, such as nerves and blood vessels. What is not widely known among the population is that diabetes may have harmful effects on the reproductive system of the men suffering from diabetes type 1 and 2 and that such a parameter could lead to or might be the reason for infertility problems for couples, for example, miscarriage or embryonic failure. AGEs is a number of products which are believed to play an important role, because their presence has been detected in increased level in diabetic men. This implies that those glycation products might play a key role in diabetic complications. Their receptor, RAGE, member of the immunoglobulin superfamily has been detected in the reproductive tract of diabetic men. Reactive oxygen species (ROS), a possible product of AGEs appear in high levels in seminal plasma and are believed to be the cause of DNA fragmentation. The objective of this review was to gather all the available material i.e. studies on diabetes mellitus in one article, to study the research which has already been conducted and the conclusions that have been drawn, in order to offer, if possible, new pathways and perspectives to the scientists, who focus on fertility problems, sometimes intractable.
文摘目的探讨晚期糖基化终产物受体(receptor of advanced glycation end products,RAGE)基因多态性与冠状动脉粥样硬化性心脏病(简称冠心病)患者单核细胞/高密度脂蛋白胆固醇比值(monocyte-to-HDL cholesterol ratio,MHR)和心率变异的相关性。方法以120名冠心病患者作为观察组,另选取同期健康体检人群120名作为对照组,分析两组以及不同严重程度患者的-429T>C、1704G>T、82G>S基因型及等位基因、MHR比值、心率变异性之间的差异,分析上述基因型与MHR比值和心率变异性的相关性。结果两组以及不同严重程度患者的RAGE基因82G>S多态性以及等位基因之间的差异有统计学意义(P<0.05)。与对照组和轻中度患者相比,观察组以及重度患者的单核细胞、总胆固醇、甘油三酯、低密度脂蛋白、MHR、低频功率显著偏高,高密度脂蛋白、NN间期标准差、RR间期平均值标准差、相邻RR间期差值、相邻RR间期差值>50 ms的心搏数占比、高频功率显著偏低。G-Gly-T、T-Gly-T、G-Ser-T、G-Gly-C的基因频率与NN间期标准差、RR间期平均值标准差、相邻RR间期差值、相邻RR间期差值>50 ms的心搏数占比、高频功率、MHR呈正相关,与低频功率呈负相关。结论冠心病患者的RAGE基因多态性与冠心病患者MHR比值和心率变异性的显著相关,可作为疾病诊断以及疗效评价的重要依据。
