Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and...Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and lubricous thalli 5–10 cm in height;(2) compressed percurrent axes bearing abundant branches with opposite arrangement;(3) claw-like apices on top, constricted to 2–4 cm at the base;(4) cortex consisting of 3–6 layers of elliptical or anomalous cells and a medulla covered by compact medullary filaments;(5) reproductive structures distributed throughout the thallus, especially centralized at the bottom of the end portion of the branches; and(6) 4-celled Carpogonial branches and 3-celled auxiliary-cell branches, both of the Grateloupia-type. The morphological diff erences were supported by molecular phylogenetics based on ribulose-1, 5-bisphosphate carboxylase/oxygenase( rbc L) gene sequence analysis. There was only a 1 bp divergence between specimens collected from Wenchang and Lingshui of Hainan province. The new species was embedded in the large Grateloupia clade of the Halymeniaceae. The pairwise distances between G. ramosa and other species within Grateloupia ranged from 26 to 105 bp, within pairwise distances of 13–111 bp between species of the large genus Grateloupia in Halymeniaceae. Thus, we propose this new species as G. ramosa Wang & Luan sp. nov.展开更多
Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe.In order to germinate,it must perceive various structurally divergent host-exuded signals,including isothiocyanates(IT...Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe.In order to germinate,it must perceive various structurally divergent host-exuded signals,including isothiocyanates(ITCs)and strigolactones(SLs).However,the receptors involved are still uncharacterized.Here,we identify five putative SL receptors in P.ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination.We demonstrate the high plasticity of PrKAI2d3,which allows it to interact with different chemicals,including ITCs.The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants.We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs.Additionally,we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P.ramosa germination with bioactivity comparable to that of ITCs.This study demonstrates that P.ramosa has extended its signal perception system during evolution,a fact that should be considered for the development of specific and efficient biocontrol methods.展开更多
以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+...以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。展开更多
基金Supported by the National Natural Science Foundation of China(Nos.3087016131270251)
文摘Grateloupia ramosa Wang & Luan sp. nov.(Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features:(1) purplish red, cartilaginous and lubricous thalli 5–10 cm in height;(2) compressed percurrent axes bearing abundant branches with opposite arrangement;(3) claw-like apices on top, constricted to 2–4 cm at the base;(4) cortex consisting of 3–6 layers of elliptical or anomalous cells and a medulla covered by compact medullary filaments;(5) reproductive structures distributed throughout the thallus, especially centralized at the bottom of the end portion of the branches; and(6) 4-celled Carpogonial branches and 3-celled auxiliary-cell branches, both of the Grateloupia-type. The morphological diff erences were supported by molecular phylogenetics based on ribulose-1, 5-bisphosphate carboxylase/oxygenase( rbc L) gene sequence analysis. There was only a 1 bp divergence between specimens collected from Wenchang and Lingshui of Hainan province. The new species was embedded in the large Grateloupia clade of the Halymeniaceae. The pairwise distances between G. ramosa and other species within Grateloupia ranged from 26 to 105 bp, within pairwise distances of 13–111 bp between species of the large genus Grateloupia in Halymeniaceae. Thus, we propose this new species as G. ramosa Wang & Luan sp. nov.
基金supported by the Institut Jean-Pierre Bourgin’s Plant Observatory technological platforms and has benefited from the facilities and expertise of the I2BC proteomic platform(Proteomic-Gif,SICaPS)supported by Infrastructures en Biologie Santéet Agronomie,Ile de France Region,Plan Cancer,CNRS,and Paris-Sud University+3 种基金The CHARM3AT Labex program(ANR-11-LABX-39)is also acknowledged for its supportthe recipient of an AgreenSkills award from the European Union in the framework of the Marie-Curie FP7 COFUND People Program and a fellowship from Saclay Plant Sciences(ANR-17-EUR-0007)A.J.is indebted to the Research Foundation Flanders for a Structural Basic Research fellowship(project 1S15817N)a travel grant in the framework of a Tournesol fellowship(project VS04418N).
文摘Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe.In order to germinate,it must perceive various structurally divergent host-exuded signals,including isothiocyanates(ITCs)and strigolactones(SLs).However,the receptors involved are still uncharacterized.Here,we identify five putative SL receptors in P.ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination.We demonstrate the high plasticity of PrKAI2d3,which allows it to interact with different chemicals,including ITCs.The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants.We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs.Additionally,we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P.ramosa germination with bioactivity comparable to that of ITCs.This study demonstrates that P.ramosa has extended its signal perception system during evolution,a fact that should be considered for the development of specific and efficient biocontrol methods.
文摘以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。
