Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate che...Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate chemical constituents, and their structures wereelucidated by extensive spectroscopic methods (UV, IR, MS, ~1H NMR, ^(13)C NMR) including 2D NMR(COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported inliterature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (2), apigenin-8-C-α-L-arabinopyranoside(3), apigenin-8-C-β-D-ga-lactopyranoside (4) , tricin-7-O-β-D-glucopyranoside (5), together withtricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11),protocatechuic acid (12) , and tematolide (13) were isolated from the 95% etha-nolic extract of itswhole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1-12were obtained from this genus and compound 13 from this species for the first time. Furthermore,compound 1 was for the first time isolated from nature while the ^(13)C NMR data of compounds 2 and3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407and A549 cell lines (IC_(45) 43, 77 μg·mL^(-1)), 8 and 10 showed inhibitory activities on KB celllines (IC_(50) 78, 44 μg·mL^(-1)) and HL-60 cell lines (Ic_(50) 85, 85 μg·mL^(-1)), while 7exerted moderate cytotoxic activities on KB, BFL-7407, A549 and HL-60 cell lines with their IC_(50)being 51, 55, 44 and 10 μg·mL^(-1) , respectively.展开更多
The preservation effects of 9 kinds of preservatives were discussed in this paper. And their effects on ornamental quality of cut Ranunculus asiaticus were in-vestigated. The results showed arranging cut Ranunculus as...The preservation effects of 9 kinds of preservatives were discussed in this paper. And their effects on ornamental quality of cut Ranunculus asiaticus were in-vestigated. The results showed arranging cut Ranunculus asiaticus in a vase fil ed with 200 mg/L 8-HQC＋1% sucrose＋75 mg/L AgNO3 would effectively slow down the weight loss, promote the rise of flower diameter, prolong life and maintain the chlorophyl and anthocyanin content of cut flower. The preservative had a good fresh-keeping effect and was suitable for promotion in actual production.展开更多
To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry ...To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry （UPLC/Q-TOF- MS） and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end, 12 alkaloids were identi- fied from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effec- tive analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established.展开更多
A novel alkenoic acid ester, （E）-4-hydroxy-dodec-2-enedioic acid-12-O-methyl ester and a new benzophenone, ethyl （S）-3-[2- （3,4-dihydroxybenzoyl）-4,5-dihydroxyphenyl]-2-hydroxypropanoate, together with a known c...A novel alkenoic acid ester, （E）-4-hydroxy-dodec-2-enedioic acid-12-O-methyl ester and a new benzophenone, ethyl （S）-3-[2- （3,4-dihydroxybenzoyl）-4,5-dihydroxyphenyl]-2-hydroxypropanoate, together with a known compound, （E）-4-hydroxy-dodec-2- enedioic acids were isolated from the roots of Ranunculus ternatus. Their structures were elucidated by spectroscopic methods.展开更多
Two new glycosides named as ternatoside A 1 and ternatoside B 2 were isolated from the roots of Ranunculus ternatus, the structures were determined by 1D and 2D NMR, ESI-MS techniques, and chemical methods.
Aims Competition for pollinators between phenotypically similar flowers is believed to play an important role in floral trait diversification in the angiosperms.However,in many plant communities,species with apparentl...Aims Competition for pollinators between phenotypically similar flowers is believed to play an important role in floral trait diversification in the angiosperms.However,in many plant communities,species with apparently similar floral phenotypes and generalist pollina-tion systems co-flower.Here,the pollination systems of Ranunculus acris L.and Ranunculus repens L.were investigated to determine the factors enabling the species to coexist within apparently over-lapping pollination niches.Methods Sympatrically flowering populations of R.acris and R.repens were investigated at three study sites in West Wales.The flo-ral phenotypes of the two species were compared using meas-urements of floral morphology and spectral analyses of petal reflectance,using principal component analysis and bee and fly colour-space models.Evidence of inter-specific discrimina-tion by foraging insects was tested for in the field and using floral arrays.The relative roles of behavioural constancy and spatial patchiness in maintaining pollinator fidelity were estimated.Important Findings The floral phenotypes of R.acris and R.repens differed significantly.Social bees were highly constant when foraging at flowers of the two species and patchy floral distribution explained some of the observed fidelity.Dipterans visiting mixed floral arrays appeared to discriminate between the species,visiting more R.acris than R.repens flowers,but there was no difference in the number of vis-its to single-species arrays.Social bees were more likely to display constancy to flowers of R.repens in the field.Patchiness in floral distribution,subtle differences in floral phenotype,pollinator preferences and behavioural constancy are all likely to con-tribute to the continued coexistence of R.acris and R.repens,despite apparent overlap in their pollination niches.Such differences have the potential to facilitate the maintenance of species diversity in plant com-munities,even where plants appear to share similar floral phenotypes.展开更多
Aims Reintroduction has become an important tool for the management of endangered plant species.We tested the little-explored effects of small-scale environmental variation,genotypic composition(i.e.identity of genoty...Aims Reintroduction has become an important tool for the management of endangered plant species.We tested the little-explored effects of small-scale environmental variation,genotypic composition(i.e.identity of genotypes)and genotypic diversity on the population survival of the regionally rare clonal plant Ranunculus reptans.For this species of periodically inundated lakeshores,genetic differentiation had been reported between populations and between short-flooded and long-flooded microsites within populations.Methods We established 306 experimental test populations at a previously unoccupied lakeshore,comprising either monocultures of 32 genotypes,mixtures of genotypes within populations or mixtures of genotypes between populations.In 2000,three years after planting out at the experimental site,a long-lasting flood caused the death of half of the experimental populations.In 2003,an extreme drought resulted in the lowest summer water levels ever measured.Important Findings Despite these climatic extremes,27%of the established populations survived in December 2003.The success of experimental populations largely differed between microsites.Moreover,the success of genotype monocultures depended on genotype and source population.Genetic differentiation between microsites played a minor role for the success of reintroduction.After the flood,populations planted with genotypes from different source populations increased in abundance,whereas populations with genotypes from single source populations and genotype monocultures decreased.In 2014,several small patches of Ranunculus clones were still present,but plants were strongly intermingled,which precluded their assignment to the original treatments.We conclude that sources for reintroductions need to be selected carefully.Moreover,mixtures of plants from different populations appear to be the best choice for successful reintroduction,at least in unpredictably varying environments.展开更多
文摘Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate chemical constituents, and their structures wereelucidated by extensive spectroscopic methods (UV, IR, MS, ~1H NMR, ^(13)C NMR) including 2D NMR(COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported inliterature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (2), apigenin-8-C-α-L-arabinopyranoside(3), apigenin-8-C-β-D-ga-lactopyranoside (4) , tricin-7-O-β-D-glucopyranoside (5), together withtricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11),protocatechuic acid (12) , and tematolide (13) were isolated from the 95% etha-nolic extract of itswhole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1-12were obtained from this genus and compound 13 from this species for the first time. Furthermore,compound 1 was for the first time isolated from nature while the ^(13)C NMR data of compounds 2 and3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407and A549 cell lines (IC_(45) 43, 77 μg·mL^(-1)), 8 and 10 showed inhibitory activities on KB celllines (IC_(50) 78, 44 μg·mL^(-1)) and HL-60 cell lines (Ic_(50) 85, 85 μg·mL^(-1)), while 7exerted moderate cytotoxic activities on KB, BFL-7407, A549 and HL-60 cell lines with their IC_(50)being 51, 55, 44 and 10 μg·mL^(-1) , respectively.
基金Supported by Jiangsu Province 333 High-level Personnel Training Project~~
文摘The preservation effects of 9 kinds of preservatives were discussed in this paper. And their effects on ornamental quality of cut Ranunculus asiaticus were in-vestigated. The results showed arranging cut Ranunculus asiaticus in a vase fil ed with 200 mg/L 8-HQC＋1% sucrose＋75 mg/L AgNO3 would effectively slow down the weight loss, promote the rise of flower diameter, prolong life and maintain the chlorophyl and anthocyanin content of cut flower. The preservative had a good fresh-keeping effect and was suitable for promotion in actual production.
文摘To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry （UPLC/Q-TOF- MS） and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end, 12 alkaloids were identi- fied from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effec- tive analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established.
文摘A novel alkenoic acid ester, （E）-4-hydroxy-dodec-2-enedioic acid-12-O-methyl ester and a new benzophenone, ethyl （S）-3-[2- （3,4-dihydroxybenzoyl）-4,5-dihydroxyphenyl]-2-hydroxypropanoate, together with a known compound, （E）-4-hydroxy-dodec-2- enedioic acids were isolated from the roots of Ranunculus ternatus. Their structures were elucidated by spectroscopic methods.
基金This research was supported by the National Natural Science Foundation of China(Grant No.30400584).
文摘Two new glycosides named as ternatoside A 1 and ternatoside B 2 were isolated from the roots of Ranunculus ternatus, the structures were determined by 1D and 2D NMR, ESI-MS techniques, and chemical methods.
文摘Aims Competition for pollinators between phenotypically similar flowers is believed to play an important role in floral trait diversification in the angiosperms.However,in many plant communities,species with apparently similar floral phenotypes and generalist pollina-tion systems co-flower.Here,the pollination systems of Ranunculus acris L.and Ranunculus repens L.were investigated to determine the factors enabling the species to coexist within apparently over-lapping pollination niches.Methods Sympatrically flowering populations of R.acris and R.repens were investigated at three study sites in West Wales.The flo-ral phenotypes of the two species were compared using meas-urements of floral morphology and spectral analyses of petal reflectance,using principal component analysis and bee and fly colour-space models.Evidence of inter-specific discrimina-tion by foraging insects was tested for in the field and using floral arrays.The relative roles of behavioural constancy and spatial patchiness in maintaining pollinator fidelity were estimated.Important Findings The floral phenotypes of R.acris and R.repens differed significantly.Social bees were highly constant when foraging at flowers of the two species and patchy floral distribution explained some of the observed fidelity.Dipterans visiting mixed floral arrays appeared to discriminate between the species,visiting more R.acris than R.repens flowers,but there was no difference in the number of vis-its to single-species arrays.Social bees were more likely to display constancy to flowers of R.repens in the field.Patchiness in floral distribution,subtle differences in floral phenotype,pollinator preferences and behavioural constancy are all likely to con-tribute to the continued coexistence of R.acris and R.repens,despite apparent overlap in their pollination niches.Such differences have the potential to facilitate the maintenance of species diversity in plant com-munities,even where plants appear to share similar floral phenotypes.
基金Swiss National Science Foundation(grants 31-49728.96,31-56809.99,31-67876.02)Bernhard Schmid and the Institute of Environmental Sciences of the University of Zurich.
文摘Aims Reintroduction has become an important tool for the management of endangered plant species.We tested the little-explored effects of small-scale environmental variation,genotypic composition(i.e.identity of genotypes)and genotypic diversity on the population survival of the regionally rare clonal plant Ranunculus reptans.For this species of periodically inundated lakeshores,genetic differentiation had been reported between populations and between short-flooded and long-flooded microsites within populations.Methods We established 306 experimental test populations at a previously unoccupied lakeshore,comprising either monocultures of 32 genotypes,mixtures of genotypes within populations or mixtures of genotypes between populations.In 2000,three years after planting out at the experimental site,a long-lasting flood caused the death of half of the experimental populations.In 2003,an extreme drought resulted in the lowest summer water levels ever measured.Important Findings Despite these climatic extremes,27%of the established populations survived in December 2003.The success of experimental populations largely differed between microsites.Moreover,the success of genotype monocultures depended on genotype and source population.Genetic differentiation between microsites played a minor role for the success of reintroduction.After the flood,populations planted with genotypes from different source populations increased in abundance,whereas populations with genotypes from single source populations and genotype monocultures decreased.In 2014,several small patches of Ranunculus clones were still present,but plants were strongly intermingled,which precluded their assignment to the original treatments.We conclude that sources for reintroductions need to be selected carefully.Moreover,mixtures of plants from different populations appear to be the best choice for successful reintroduction,at least in unpredictably varying environments.
