目的:探讨mTOR调节相关蛋白(regulatory-associated protein of mTOR,RAPTOR)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)迁移、侵袭和增殖能力的影响。方法:利用TCGA生物信息数据库查询在头颈部鳞状细胞癌(head and neck squ...目的:探讨mTOR调节相关蛋白(regulatory-associated protein of mTOR,RAPTOR)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)迁移、侵袭和增殖能力的影响。方法:利用TCGA生物信息数据库查询在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织与癌旁组织中差异表达的mRNA。Western blot检测RAPTOR在人口腔上皮细胞HOEC和OSCC细胞系中的表达。利用伤口愈合实验、Transwell实验、EdU实验检测各组细胞迁移、侵袭及增殖能力。生物信息学网站预测与RAPTOR靶向结合的微小RNA(microRNA,miR)。功能学实验验证miR-485-5p是否可以靶向RAPTOR影响OSCC细胞的迁移、侵袭和增殖。结果:RAPTOR在HNSCC组织中较癌旁组织表达增高。伤口愈合、Transwell和EdU实验结果示,RAPTOR有促进OSCC细胞的迁移、侵袭和增殖能力。miR-485-5p能与RAPTOR靶向结合,且miR-485-5p上调能逆转RAPTOR促进CAL27细胞迁移、侵袭和增殖能力。结论:miR-485-5p通过靶向RAPTOR抑制OSCC细胞迁移、侵袭和增殖能力。展开更多
Raptors are getting more attention from researchers because of their excellent flight abilities.And the excellent wing morphing ability is critical for raptors to achieve high maneuvering flight,which can be a good bi...Raptors are getting more attention from researchers because of their excellent flight abilities.And the excellent wing morphing ability is critical for raptors to achieve high maneuvering flight,which can be a good bionic inspiration for unmanned aerial vehicles(UAV)design.However,morphing wing motions of Falco peregrinus with multi postures cannot be consulted since such a motion database was nonexistent.This study aimed to provide data reference for future research in wing morphing kinetics.We used the computed tomography(CT)approach to obtain nine critical postures of the Falco peregrinus wing skeleton,followed with motion analysis of each joint and bone.Based on the obtained motion database,a six-bar kinematic model was proposed to regenerate wing motions with a high fidelity.展开更多
针对水声通信带宽低、时延长、误码率高、多普勒效应显著等特征,分析了传统可靠传输机制在水下传感器网络应用的局限性,提出了基于数字喷泉码-优化Raptor码的水下传感器网络可靠传输机制。采用反馈控制对Raptor码内码的鲁棒孤子分布和...针对水声通信带宽低、时延长、误码率高、多普勒效应显著等特征,分析了传统可靠传输机制在水下传感器网络应用的局限性,提出了基于数字喷泉码-优化Raptor码的水下传感器网络可靠传输机制。采用反馈控制对Raptor码内码的鲁棒孤子分布和多项式描述的Shokrollahi度分布进行优化设计,降低了编码包平均度。进一步对Raptor码的内码—弱化的LT码(Luby transform codes)的编解码进行优化,实现了快速的编解码。基于优化Raptor码的可靠传输机制采用反馈控制,动态评估信道删除概率,从而提高编解码和通信效率。通过Aqua-Sim仿真工具对提出的可靠传输机制与基于编码的多跳协同可靠数据传输(coding based multi-hop coordinated reliable data transfer,CCRDT)机制进行仿真对比。结果表明,所提出的可靠传输机制明显降低了传输开销,提高了数据吞吐量。展开更多
文摘目的:探讨mTOR调节相关蛋白(regulatory-associated protein of mTOR,RAPTOR)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)迁移、侵袭和增殖能力的影响。方法:利用TCGA生物信息数据库查询在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织与癌旁组织中差异表达的mRNA。Western blot检测RAPTOR在人口腔上皮细胞HOEC和OSCC细胞系中的表达。利用伤口愈合实验、Transwell实验、EdU实验检测各组细胞迁移、侵袭及增殖能力。生物信息学网站预测与RAPTOR靶向结合的微小RNA(microRNA,miR)。功能学实验验证miR-485-5p是否可以靶向RAPTOR影响OSCC细胞的迁移、侵袭和增殖。结果:RAPTOR在HNSCC组织中较癌旁组织表达增高。伤口愈合、Transwell和EdU实验结果示,RAPTOR有促进OSCC细胞的迁移、侵袭和增殖能力。miR-485-5p能与RAPTOR靶向结合,且miR-485-5p上调能逆转RAPTOR促进CAL27细胞迁移、侵袭和增殖能力。结论:miR-485-5p通过靶向RAPTOR抑制OSCC细胞迁移、侵袭和增殖能力。
基金supported by the National Natural Science Foundation of China(Grant Nos.52175279,52075489,and 51705459)the Natural Science Foundation of Zhejiang Province,China(Grant Nos.LY20E050022 and LGG20E050017)。
文摘Raptors are getting more attention from researchers because of their excellent flight abilities.And the excellent wing morphing ability is critical for raptors to achieve high maneuvering flight,which can be a good bionic inspiration for unmanned aerial vehicles(UAV)design.However,morphing wing motions of Falco peregrinus with multi postures cannot be consulted since such a motion database was nonexistent.This study aimed to provide data reference for future research in wing morphing kinetics.We used the computed tomography(CT)approach to obtain nine critical postures of the Falco peregrinus wing skeleton,followed with motion analysis of each joint and bone.Based on the obtained motion database,a six-bar kinematic model was proposed to regenerate wing motions with a high fidelity.
文摘针对水声通信带宽低、时延长、误码率高、多普勒效应显著等特征,分析了传统可靠传输机制在水下传感器网络应用的局限性,提出了基于数字喷泉码-优化Raptor码的水下传感器网络可靠传输机制。采用反馈控制对Raptor码内码的鲁棒孤子分布和多项式描述的Shokrollahi度分布进行优化设计,降低了编码包平均度。进一步对Raptor码的内码—弱化的LT码(Luby transform codes)的编解码进行优化,实现了快速的编解码。基于优化Raptor码的可靠传输机制采用反馈控制,动态评估信道删除概率,从而提高编解码和通信效率。通过Aqua-Sim仿真工具对提出的可靠传输机制与基于编码的多跳协同可靠数据传输(coding based multi-hop coordinated reliable data transfer,CCRDT)机制进行仿真对比。结果表明,所提出的可靠传输机制明显降低了传输开销,提高了数据吞吐量。
