Based on the investigation of the luminescence of a series of rare earth organic chelates, some relationships between luminescence and the structure of the chelates were proposed: the intensity of sensitized luminesce...Based on the investigation of the luminescence of a series of rare earth organic chelates, some relationships between luminescence and the structure of the chelates were proposed: the intensity of sensitized luminescence of central lanthanide ions(Ln 3+ ) in a rare earth organic chelate depends on (1)the suitability of the energy gap between the excited triplet energy level of the ligands and the lowest excited energy level of Ln 3+ ions; (2)the rigidity and planarity of the structure of the chelate molecule; (3)the existence of a suitable secondary ligand which may increase rigidity and the stability of the chelate molecule; and (4) the existence of a suitable π conjugated system in the chelate molecule. According to the above relationships, 25 novel organic ligands were designed and synthesized, and their lanthanide chelates were prepared. Investigation of the photoluminescence for the new chelates shows that some of the chelates are strongly luminescent, and are applied to fluoroimmunoassay for determination of human immunoglobulin(IgG), to preparation of fluorescent plastics, and to determination of growth hormone for plants. Two novel spectroscopy probe techniques for structure of coordination compounds and biological molecules were proposed and developed based on vibronic spectroscopy of Tb 3+ complexes and fluorescence of Ce 3+ .展开更多
The kinetic behavior of forming the iso-nuclear β-type chelates of rare earth ions (RE~3+) with p-sulphoaminobromophosphonazo (BPA-pSN) in ClCH2COOH-CH3COONa buffer solutions were studied by a spectrophotometric method.
Proton ligand stability constants of hydroxybenzoic acid containing nitro group （2-Hydroxy-4-nitrobenzoic acid and 3-Hydroxy-4- nitrobenzoic acid） were determined through lrving-Rossoti pH titration technique. The s...Proton ligand stability constants of hydroxybenzoic acid containing nitro group （2-Hydroxy-4-nitrobenzoic acid and 3-Hydroxy-4- nitrobenzoic acid） were determined through lrving-Rossoti pH titration technique. The stability constants of rare earth metal chelates containing 2-Hydroxy-4-nitrobenzoic acid and 3-Hydroxy-4-nitrobenzoic acid as ligands were studied in aqueous medium at different ionic strength （p=0.01, 0.05 and 0.1 mol/L） and temperatures （298, 308 and 318± 0.1 K）. The values of stability constant were evaluated through different computational methods like successive approximation, least square treatment, correction term method, and convergence method. The observed value of thermodynamic parameters （△G°, △H° and △S°） favors the complex formation between metal and ligands. The order of stabifity constant was found to be Pr（III）〈Nd（III）〈Sm（III）〈Tb（III）〈Ho（III）〈Er（III） as reported by Stagg and Powell.展开更多
Macroporous amino-carboxvlic chelating resin of acrylic acid series may concentrate trace rare earth in the presence of 0.1 mol/l α-hydroxy-isobutyric acid at pH 4.5.With 0.10 mol/l HCl as eluant rare earth may be el...Macroporous amino-carboxvlic chelating resin of acrylic acid series may concentrate trace rare earth in the presence of 0.1 mol/l α-hydroxy-isobutyric acid at pH 4.5.With 0.10 mol/l HCl as eluant rare earth may be eluted quantitatively and separated from Ca,Mg,Fe and Cu.This method is used to determine trace amount of rare earth in human hair,stone (in kidney and bladder),peanut,grape,strawberry,cucumber,pig liver and other biological samples.展开更多
A novel high sensihtity, small-volume photothermal intheence detector has beenintroduced for capillap zone electrophoresis separation analysis. The utility of thes sdriulboconstecheque for momtomp chelating reachon of...A novel high sensihtity, small-volume photothermal intheence detector has beenintroduced for capillap zone electrophoresis separation analysis. The utility of thes sdriulboconstecheque for momtomp chelating reachon of light rare earth with tribromoarsenazo has beenreported.展开更多
LaCrO 3 thin films were successfully prepared at lower temperatures by the sol gel process based on organic chelating precursors. The formation and transition of the gel derived from the chelate solution were analyz...LaCrO 3 thin films were successfully prepared at lower temperatures by the sol gel process based on organic chelating precursors. The formation and transition of the gel derived from the chelate solution were analyzed by means of IR, DTA TG, XRD and TEM. In addition, the formation and microstructure of thin films were investigated by SEM and XRD. The results show that the gel formed by the condensation reaction is decomposed at about 195 ℃, transformed into a great quantity of LaCrO 3 by heat treating at 200 ℃ for 1 h, and the complexing ways in the gel have an important influence upon the formation of LaCrO 3. The formation process of thin film includes the nucleation and growth on the substrate, the formation of polycrystalline islands and the continuous film by repeating the dip coating process. Higher heat treatment temperature gives rise to larger granules in the thin film.展开更多
Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal anti...Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal antibodies （McAbs） coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-（p-isothiocyanatobenzyl）-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3＋ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.展开更多
文摘Based on the investigation of the luminescence of a series of rare earth organic chelates, some relationships between luminescence and the structure of the chelates were proposed: the intensity of sensitized luminescence of central lanthanide ions(Ln 3+ ) in a rare earth organic chelate depends on (1)the suitability of the energy gap between the excited triplet energy level of the ligands and the lowest excited energy level of Ln 3+ ions; (2)the rigidity and planarity of the structure of the chelate molecule; (3)the existence of a suitable secondary ligand which may increase rigidity and the stability of the chelate molecule; and (4) the existence of a suitable π conjugated system in the chelate molecule. According to the above relationships, 25 novel organic ligands were designed and synthesized, and their lanthanide chelates were prepared. Investigation of the photoluminescence for the new chelates shows that some of the chelates are strongly luminescent, and are applied to fluoroimmunoassay for determination of human immunoglobulin(IgG), to preparation of fluorescent plastics, and to determination of growth hormone for plants. Two novel spectroscopy probe techniques for structure of coordination compounds and biological molecules were proposed and developed based on vibronic spectroscopy of Tb 3+ complexes and fluorescence of Ce 3+ .
基金a grant from the Educational Commission of Gansu Province.
文摘The kinetic behavior of forming the iso-nuclear β-type chelates of rare earth ions (RE~3+) with p-sulphoaminobromophosphonazo (BPA-pSN) in ClCH2COOH-CH3COONa buffer solutions were studied by a spectrophotometric method.
文摘Proton ligand stability constants of hydroxybenzoic acid containing nitro group （2-Hydroxy-4-nitrobenzoic acid and 3-Hydroxy-4- nitrobenzoic acid） were determined through lrving-Rossoti pH titration technique. The stability constants of rare earth metal chelates containing 2-Hydroxy-4-nitrobenzoic acid and 3-Hydroxy-4-nitrobenzoic acid as ligands were studied in aqueous medium at different ionic strength （p=0.01, 0.05 and 0.1 mol/L） and temperatures （298, 308 and 318± 0.1 K）. The values of stability constant were evaluated through different computational methods like successive approximation, least square treatment, correction term method, and convergence method. The observed value of thermodynamic parameters （△G°, △H° and △S°） favors the complex formation between metal and ligands. The order of stabifity constant was found to be Pr（III）〈Nd（III）〈Sm（III）〈Tb（III）〈Ho（III）〈Er（III） as reported by Stagg and Powell.
文摘Macroporous amino-carboxvlic chelating resin of acrylic acid series may concentrate trace rare earth in the presence of 0.1 mol/l α-hydroxy-isobutyric acid at pH 4.5.With 0.10 mol/l HCl as eluant rare earth may be eluted quantitatively and separated from Ca,Mg,Fe and Cu.This method is used to determine trace amount of rare earth in human hair,stone (in kidney and bladder),peanut,grape,strawberry,cucumber,pig liver and other biological samples.
文摘A novel high sensihtity, small-volume photothermal intheence detector has beenintroduced for capillap zone electrophoresis separation analysis. The utility of thes sdriulboconstecheque for momtomp chelating reachon of light rare earth with tribromoarsenazo has beenreported.
文摘LaCrO 3 thin films were successfully prepared at lower temperatures by the sol gel process based on organic chelating precursors. The formation and transition of the gel derived from the chelate solution were analyzed by means of IR, DTA TG, XRD and TEM. In addition, the formation and microstructure of thin films were investigated by SEM and XRD. The results show that the gel formed by the condensation reaction is decomposed at about 195 ℃, transformed into a great quantity of LaCrO 3 by heat treating at 200 ℃ for 1 h, and the complexing ways in the gel have an important influence upon the formation of LaCrO 3. The formation process of thin film includes the nucleation and growth on the substrate, the formation of polycrystalline islands and the continuous film by repeating the dip coating process. Higher heat treatment temperature gives rise to larger granules in the thin film.
文摘Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ （PGI） with time-resolved fluoroimmunoassay （TRFIA） as a detection technique. On the noncompetitive assay, captured monoclonal antibodies （McAbs） coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-（p-isothiocyanatobenzyl）-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3＋ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.
