The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Z...The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology.展开更多
Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communiti...Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand.展开更多
The rare microbial biosphere provides broad ecological services and resilience to various ecosystems.Nevertheless,the biogeographical patterns and assembly processes of rare bacterioplankton communities in large river...The rare microbial biosphere provides broad ecological services and resilience to various ecosystems.Nevertheless,the biogeographical patterns and assembly processes of rare bacterioplankton communities in large rivers remain uncertain.In this study,we investigated the biogeography and community assembly processes of abundant and rare bacterioplankton taxa in the Yangtze River(China)covering a distance of 4300 km.The results revealed similar spatiotemporal patterns of abundant taxa(AT)and rare taxa(RT)at both taxonomic and phylogenetic levels,and analysis of similarities revealed that RT was significantly influenced by season and landform than AT.Furthermore,RT correlated with more environmental factors than AT,whereas environmental and spatial factors explained a lower proportion of community shifts in RT than in AT.The steeper distance–decay slopes in AT indicated higher spatial turnover rates of abundant subcommunities than rare subcommunities.The null model revealed that both AT and RT were mainly governed by stochastic processes.However,dispersal limitation primarily governed the AT,whereas the undominated process accounted for a higher fraction of stochastic processes in RT.River flow and suspended solids mediated the balance between the stochastic and deterministic processes in RT.The spatiotemporal dynamics and assembly processes of total taxa were more similar as AT than RT.This study provides new insights into both significant spatiotemporal dynamics and inconsistent assembly processes of AT and RT in large rivers.展开更多
基金supported by the Cooperation Project of Luzhou Laojiao Co.,Ltd.Sichuan University (21H0997)。
文摘The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology.
基金the Sino-German Cooperation Forestry Major Scientific Research Project(zdczhz2021ky09)the National Natural Science Foundation of China(31971487 and 42277245).
文摘Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand.
基金supported by National Natural Science Foundation of China(Grant No.41907203)China Postdoctoral Science Foundation(202IT 140010).
文摘The rare microbial biosphere provides broad ecological services and resilience to various ecosystems.Nevertheless,the biogeographical patterns and assembly processes of rare bacterioplankton communities in large rivers remain uncertain.In this study,we investigated the biogeography and community assembly processes of abundant and rare bacterioplankton taxa in the Yangtze River(China)covering a distance of 4300 km.The results revealed similar spatiotemporal patterns of abundant taxa(AT)and rare taxa(RT)at both taxonomic and phylogenetic levels,and analysis of similarities revealed that RT was significantly influenced by season and landform than AT.Furthermore,RT correlated with more environmental factors than AT,whereas environmental and spatial factors explained a lower proportion of community shifts in RT than in AT.The steeper distance–decay slopes in AT indicated higher spatial turnover rates of abundant subcommunities than rare subcommunities.The null model revealed that both AT and RT were mainly governed by stochastic processes.However,dispersal limitation primarily governed the AT,whereas the undominated process accounted for a higher fraction of stochastic processes in RT.River flow and suspended solids mediated the balance between the stochastic and deterministic processes in RT.The spatiotemporal dynamics and assembly processes of total taxa were more similar as AT than RT.This study provides new insights into both significant spatiotemporal dynamics and inconsistent assembly processes of AT and RT in large rivers.
基金supported by the Science and Technology Program of Inner Mongolia Autonomous Region of China (2021GG0307,2020GG0126)the Science and Technology Major Project of Inner Mongolia Autonomous Region of China (2021ZD0011-1).
