RAVE(regulator of the H+-ATPase of the vacuolar and endosomal membrane)由Rav1p、Rav2p和Skp1p3个亚基组成,是V-ATP酶活性调节机制中一个关键的多亚基复合物调节因子;Leu1p能够与RAVE相互作用。通过融合PCR、同源重组分别构建了酿...RAVE(regulator of the H+-ATPase of the vacuolar and endosomal membrane)由Rav1p、Rav2p和Skp1p3个亚基组成,是V-ATP酶活性调节机制中一个关键的多亚基复合物调节因子;Leu1p能够与RAVE相互作用。通过融合PCR、同源重组分别构建了酿酒酵母BY4742的Rav1、Rav2、Leu1和Vma2缺陷型菌株。进一步研究重组菌株对p H及Ca Cl2的耐受能力发现:Leu1缺陷型菌株(3-异丙基苹果酸异构酶(Leu1p)缺失)的生长状况与Rav1、Rav2缺陷型菌株的生长状况基本一致,表明RAVE对V-ATP酶的活性调节极有可能需要通过与Leu1p结合才能实现。笔者所在实验室为进一步研究RAVE与Leu1p的相互关系及其对V-ATP酶的活性调节提供了重要依据。展开更多
RAVE （regulator of the H ^＋ -ATPase of the vacuolar and endosomal membranes）复合物由Rav1p、Rav2p和Skp1p3（DE3）中进行表达。通过IPTG诱导，SDS-PAGE分析重组菌在诱导后表达出目的蛋白。目的蛋白经Ni-NTA凝胶纯化后再经质谱...RAVE （regulator of the H ^＋ -ATPase of the vacuolar and endosomal membranes）复合物由Rav1p、Rav2p和Skp1p3（DE3）中进行表达。通过IPTG诱导，SDS-PAGE分析重组菌在诱导后表达出目的蛋白。目的蛋白经Ni-NTA凝胶纯化后再经质谱进一步验证确定为酿酒酵母的Rav2p蛋白。目前国际上还没有有关Rav2p的结构和性质以及RAVE亚基之间相互关系的研究。重组质粒pETDuet-R2的成功构建以及Rav2p的可溶性表达为研究RAVE亚基之间的相互作用以及V-ATP酶的活性调节机理打下基础。展开更多
Concentrations and distribution patterns of rare earth elements(REEs)in the intertidal flat of Tianjin were studied using P(507) resin and ICP-MS analytical technology. The results show that average concentrations of ...Concentrations and distribution patterns of rare earth elements(REEs)in the intertidal flat of Tianjin were studied using P(507) resin and ICP-MS analytical technology. The results show that average concentrations of REEs in the filtered(dissolved REEs), unfiltered and interstitial water are 0.461 μg·L^(-1), 4.98 μg·L^(-1) and 0.845 μg·L^(-1), respectively. Their distribution patterns are similar to those in inland natural rivers, but quite different from those in oceans. The average concentrations of REEs in sediments and suspended matters are 161.2 (mg·L^(-1)) and 168.1 mg·L^(-1), respectively, which are much lower than those in sediments of natural rivers but higher than those in sediments of the continental shelf of East China Sea. It is suggested that REEs in sediments and suspended matters of intertidal flat can have mainly originated from terrestrial soils and thus their transportation and transformation processes are similar with those in rivers, and different from those in continental shelves. Pollutants discharged into the Bohai Bay do·not cause significant effect on REEs in the intertidal flat of Tianjin yet.展开更多
文摘RAVE(regulator of the H+-ATPase of the vacuolar and endosomal membrane)由Rav1p、Rav2p和Skp1p3个亚基组成,是V-ATP酶活性调节机制中一个关键的多亚基复合物调节因子;Leu1p能够与RAVE相互作用。通过融合PCR、同源重组分别构建了酿酒酵母BY4742的Rav1、Rav2、Leu1和Vma2缺陷型菌株。进一步研究重组菌株对p H及Ca Cl2的耐受能力发现:Leu1缺陷型菌株(3-异丙基苹果酸异构酶(Leu1p)缺失)的生长状况与Rav1、Rav2缺陷型菌株的生长状况基本一致,表明RAVE对V-ATP酶的活性调节极有可能需要通过与Leu1p结合才能实现。笔者所在实验室为进一步研究RAVE与Leu1p的相互关系及其对V-ATP酶的活性调节提供了重要依据。
文摘RAVE （regulator of the H ^＋ -ATPase of the vacuolar and endosomal membranes）复合物由Rav1p、Rav2p和Skp1p3（DE3）中进行表达。通过IPTG诱导，SDS-PAGE分析重组菌在诱导后表达出目的蛋白。目的蛋白经Ni-NTA凝胶纯化后再经质谱进一步验证确定为酿酒酵母的Rav2p蛋白。目前国际上还没有有关Rav2p的结构和性质以及RAVE亚基之间相互关系的研究。重组质粒pETDuet-R2的成功构建以及Rav2p的可溶性表达为研究RAVE亚基之间的相互作用以及V-ATP酶的活性调节机理打下基础。
文摘Concentrations and distribution patterns of rare earth elements(REEs)in the intertidal flat of Tianjin were studied using P(507) resin and ICP-MS analytical technology. The results show that average concentrations of REEs in the filtered(dissolved REEs), unfiltered and interstitial water are 0.461 μg·L^(-1), 4.98 μg·L^(-1) and 0.845 μg·L^(-1), respectively. Their distribution patterns are similar to those in inland natural rivers, but quite different from those in oceans. The average concentrations of REEs in sediments and suspended matters are 161.2 (mg·L^(-1)) and 168.1 mg·L^(-1), respectively, which are much lower than those in sediments of natural rivers but higher than those in sediments of the continental shelf of East China Sea. It is suggested that REEs in sediments and suspended matters of intertidal flat can have mainly originated from terrestrial soils and thus their transportation and transformation processes are similar with those in rivers, and different from those in continental shelves. Pollutants discharged into the Bohai Bay do·not cause significant effect on REEs in the intertidal flat of Tianjin yet.
