The various behaviors of four Amur tigers in captivity were investigated in March from 1998 to 2001 in Harbin Zoo. The results showed that the behavior could be divided into 5 major types: moving, resting, sleeping, e...The various behaviors of four Amur tigers in captivity were investigated in March from 1998 to 2001 in Harbin Zoo. The results showed that the behavior could be divided into 5 major types: moving, resting, sleeping, eating and other behaviors (including drinking, urinating, grooming, playing, standing). Of all behavioral models, sleeping and moving behaviors alone accounted for 75.18% and were two major behavioral models of all behaviors. Resting, eating and other behaviors accounted for 24.82%. Apart from eating, one male and two female individuals have similar peak periods of 4 major behaviors (one peak period in the daytime, and one peak period at night), similar to that of wild Amur tiger. However, one female individual have two clear peak periods at night.展开更多
In modern wildlife ecological research,feces is the most common non-invasive source of DNA obtained in the field and polymerase chain reaction(PCR) technology based on microsatellite markers is used to mine genetic in...In modern wildlife ecological research,feces is the most common non-invasive source of DNA obtained in the field and polymerase chain reaction(PCR) technology based on microsatellite markers is used to mine genetic information contained within.This is especially the case for endangered species.However,there are risks associated with this genotyping method because of the poor quality of fecal DNA.In this study,we assessed genotyping risk across 12 microsatellite loci commonly used in previous tiger studies using blood and fecal DNA from captive Amur tigers(Panthera tigris altaica).To begin,we developed an index termed the accumulated matching rate of genotypes(R)between positive DNA(blood samples) and fecal DNA to explore the correct genotyping probability of a certain microsatellite locus.We found that different microsatelliteloci had different genotyping risks and required different PCR amplification protocols.The genotyping errors we detected altered population genetic parameters and potentially impact subsequent analyses.Based on these findings,we recommend that:(1) four loci(E7,Fca094,Pti007 and Pti010) of 12 loci are not suitable for Amur tiger genetic research because of a low Rand difficulty reaching a stable status;(2) the Rof the 12 microsatellite loci plateaued differently,and considering limited budgets,amplification times of some loci could be increased when using fecal samples; and(3) future genetic analysis of wild Amur tigers should be corrected by genotyping error rates(1-R).展开更多
基金This paper was supported by the National Forestry Bureau (97-27).
文摘The various behaviors of four Amur tigers in captivity were investigated in March from 1998 to 2001 in Harbin Zoo. The results showed that the behavior could be divided into 5 major types: moving, resting, sleeping, eating and other behaviors (including drinking, urinating, grooming, playing, standing). Of all behavioral models, sleeping and moving behaviors alone accounted for 75.18% and were two major behavioral models of all behaviors. Resting, eating and other behaviors accounted for 24.82%. Apart from eating, one male and two female individuals have similar peak periods of 4 major behaviors (one peak period in the daytime, and one peak period at night), similar to that of wild Amur tiger. However, one female individual have two clear peak periods at night.
基金financially supported by Fundamental Research Funds for the Central Universities of China(2572014EA06)National Natural Science Foundation of China(NSFC 31572285)Study on Resource Survey Technology for Tiger and Amur Leopard Population(State Forestry Administration)
文摘In modern wildlife ecological research,feces is the most common non-invasive source of DNA obtained in the field and polymerase chain reaction(PCR) technology based on microsatellite markers is used to mine genetic information contained within.This is especially the case for endangered species.However,there are risks associated with this genotyping method because of the poor quality of fecal DNA.In this study,we assessed genotyping risk across 12 microsatellite loci commonly used in previous tiger studies using blood and fecal DNA from captive Amur tigers(Panthera tigris altaica).To begin,we developed an index termed the accumulated matching rate of genotypes(R)between positive DNA(blood samples) and fecal DNA to explore the correct genotyping probability of a certain microsatellite locus.We found that different microsatelliteloci had different genotyping risks and required different PCR amplification protocols.The genotyping errors we detected altered population genetic parameters and potentially impact subsequent analyses.Based on these findings,we recommend that:(1) four loci(E7,Fca094,Pti007 and Pti010) of 12 loci are not suitable for Amur tiger genetic research because of a low Rand difficulty reaching a stable status;(2) the Rof the 12 microsatellite loci plateaued differently,and considering limited budgets,amplification times of some loci could be increased when using fecal samples; and(3) future genetic analysis of wild Amur tigers should be corrected by genotyping error rates(1-R).
