Despite the economic importance of the peanut, no studies have been carried out to deter-mine the correlation between genetic distances based on molecular markers and on coefficient of parentage （COP） data. In ...Despite the economic importance of the peanut, no studies have been carried out to deter-mine the correlation between genetic distances based on molecular markers and on coefficient of parentage （COP） data. In this study, simple sequence repeat （SSR） and pedigree data were used to assess the ge-netic distance between the Fuhuasheng variety and its derivative cultivars. A total of 39 SSR polymor-phism primers were used, and 151 bands were obtained, with an average of 2. 04 bands in each primer. The genetic SSR - based distance （GD） values ranged from 0. 02 to 0. 81, while the COP - based GD ranged from 0. 25 to 0. 98. Certain Fuhuasheng loci displayed higher transmission rates. These loci or nearby chromosomal regions might be associated with desirable traits in Fuhuasheng variety, thus being frequently selected in breeding programs. Therefore, it can be suggested that COP analysis should be the preferred method for estimating genetic diversity invarieties with available complete pedigree information and parents. In this case, marker analysis would provide the best estimations.展开更多
采用FIASCO(Fast Isolation by AFLP of Sequences Containing repeats site)方法富集、分离海洋产毒甲藻——塔玛亚历山大藻基因组DNA微卫星位点。结果发现,测序分析的22个克隆中全部含有微卫星位点,插入片段大小为175-687 bp,微卫星DN...采用FIASCO(Fast Isolation by AFLP of Sequences Containing repeats site)方法富集、分离海洋产毒甲藻——塔玛亚历山大藻基因组DNA微卫星位点。结果发现,测序分析的22个克隆中全部含有微卫星位点,插入片段大小为175-687 bp,微卫星DNA的重复数最低4次,最高27次,平均11次;在全部的39个微卫星位点中,其中25个属于完美重复型,13个不完美重复型,1个复合型。为塔玛亚历山大藻系统进化生物学、分子生态学研究提供了很好的标记位点。展开更多
基金This work was supported by a grant from the Major State Basic Research Development Program of China （973 Program）（No.2011CB109304 ）； the National Natural Science Foundation of China （No. 30571132）；National Science ＆ Technology Pillar Pro-gram （2006BAD13B05- 2）； Plant Germplasm Re-sources Sharing Platform （2005DKA21002 - 13）； Di-rector Foundation of the Institute Oil Crop Research, CAAS（1610172011004）.
文摘Despite the economic importance of the peanut, no studies have been carried out to deter-mine the correlation between genetic distances based on molecular markers and on coefficient of parentage （COP） data. In this study, simple sequence repeat （SSR） and pedigree data were used to assess the ge-netic distance between the Fuhuasheng variety and its derivative cultivars. A total of 39 SSR polymor-phism primers were used, and 151 bands were obtained, with an average of 2. 04 bands in each primer. The genetic SSR - based distance （GD） values ranged from 0. 02 to 0. 81, while the COP - based GD ranged from 0. 25 to 0. 98. Certain Fuhuasheng loci displayed higher transmission rates. These loci or nearby chromosomal regions might be associated with desirable traits in Fuhuasheng variety, thus being frequently selected in breeding programs. Therefore, it can be suggested that COP analysis should be the preferred method for estimating genetic diversity invarieties with available complete pedigree information and parents. In this case, marker analysis would provide the best estimations.
文摘采用FIASCO(Fast Isolation by AFLP of Sequences Containing repeats site)方法富集、分离海洋产毒甲藻——塔玛亚历山大藻基因组DNA微卫星位点。结果发现,测序分析的22个克隆中全部含有微卫星位点,插入片段大小为175-687 bp,微卫星DNA的重复数最低4次,最高27次,平均11次;在全部的39个微卫星位点中,其中25个属于完美重复型,13个不完美重复型,1个复合型。为塔玛亚历山大藻系统进化生物学、分子生态学研究提供了很好的标记位点。
