Ecological toxicity of reactive X\|3B red dye and cadmium in both their single form and their combined form on wheat was studied using the experimental method of seed and root exposure. The single factor exposure ind...Ecological toxicity of reactive X\|3B red dye and cadmium in both their single form and their combined form on wheat was studied using the experimental method of seed and root exposure. The single factor exposure indicated that the inhibitory rate of wheat root elongation was significantly increased with the increase in the concentration of the dye in the cultural solution, although seed germination of wheat was not sensitive to the dye. The toxicity of cadmium was greatly higher than that of the dye, but low concentration cadmium (<40 mg/L) could promote the germination of wheat seed. Interactive effects of the dye and cadmium on wheat were complicated. There was no significant correlation between the inhibitory rate of seed germination and the concentrations of the dye and cadmium. Low concentration cadmium could strengthen the toxicity of the dye acting on root elongation. On the contrary, high concentration cadmium could weaken the toxicity of the dye acting on root elongation.展开更多
以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactiv...以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactive Red241模拟染料废水的脱色率可达到86%以上;在p H=7,U=14V、I=3.2A、t=25min的条件下,C.I.Disperse Blue 56模拟染料废水的脱色率可达到79以上%。展开更多
One of the new directions in the field of Cretaceous research is to elucidate the mechanism of the sedimentary transition from the Cretaceous black shales to oceanic red beds. A chemical sequential extraction method w...One of the new directions in the field of Cretaceous research is to elucidate the mechanism of the sedimentary transition from the Cretaceous black shales to oceanic red beds. A chemical sequential extraction method was applied to these two types of rocks from southern Tibet to investigate the burial records of reactive iron. Results indicate that carbonate-associated iron and pyrite are relatively enriched in the black shales, but depleted or absent in red beds. The main feature of the reactive iron in the red beds is relative enrichment of iron oxides (largely hematite), which occurred during syn-depostion or early diagenesis. The ratio between iron oxides and the total iron indicates an oxygen-enriched environment for red bed deposition. A comparison between the reactive iron burial records and proxies of paleo-productivity suggests that paleo-productivity decreases when the ratio between iron oxides and the total iron increases in the red beds. This phenomenon could imply that the relationship between marine redox and productivity might be one of the reasons for the sedimentary transition from Cretaceous black shale to oceanic red bed deposition.展开更多
A comparative study of treatment of simulated wastewater containing Reactive Red 195 using zero-valent iron/activated carbon (ZVI/AC), microwave discharge electrodeless lamp/sodium hypochlorite (MDEL/NaCIO) and th...A comparative study of treatment of simulated wastewater containing Reactive Red 195 using zero-valent iron/activated carbon (ZVI/AC), microwave discharge electrodeless lamp/sodium hypochlorite (MDEL/NaCIO) and the combination of ZVI/AC- MDEL/NaCIO was conducted. The preliminary results showed the two steps method of ZVI/AC-MDEL/NaCIO had much higher degradation efficiency than both single steps. The final color removal percentage was nearly up to 100% and the chemical oxygen demand reduction percentage was up to approximately 82%. The effects of operational parameters, including initial pH value of simulated wastewater, ZVI/AC ratio and particle size of ZVI were also investigated. In addition, from the discussion of synergistic effect between ZVI/AC and MEDL/NaCIO, we found that in the ZVI/AC-MEDL/NaCIO process, ZVI/AC could break the azo bond firstly and then MEDLfNaCIO degraded the aromatic amine products effectively. Reversing the order would reduce the degradation efficiency.展开更多
The mechanism of sedimentary transition from the Cretaceous black shales to the oceanic red beds is a new and important direction of Cretaceous research. Chemical sequential extraction is applied to study the burial r...The mechanism of sedimentary transition from the Cretaceous black shales to the oceanic red beds is a new and important direction of Cretaceous research. Chemical sequential extraction is applied to study the burial records of reactive phosphorus in the black shale of the Gyabula Formation and oceanic red beds of the Chuangde Formation, Southern Tibet. Results indicate that the principal reactive phosphorus species is the authigenic and carbonate-associated phosphorus (CAP) in the Gyabula Formation and iron oxides-associated phosphorus (FeP) in the Chuangde Formation which accounts for more than half of their own total phosphorus content. While the authigenic and carbonate-associated phosphorus (CAP) is almost equal in the two Formations; the iron oxidesassociated phosphorus is about 1.6 times higher in the Chuangde Formation than that in the Gyabula Formation resulting in a higher content of the total phosphorus in the Chuangde Formation. According to the observations on the marine phosphorus cyde in Modern Ocean, it is found that preferential burial and regeneration of reactive phosphorus corresponds to highly oxic and reducing conditions, respectively, leading to the different distribution of phosphorus in these two distinct type of marine sediments. It is the redox-sensitive behavior of phosphorus cycle to the different redox conditions in the ocean and the controlling effects of phosphorus to the marine production that stimulate the local sedimentary transition from the Cretaceous black shale to the oceanic red beds.展开更多
Almost all the studies both domestic and international using white rot fungus for dye wastewater treatment are performed under sterile conditions. However, it is obviously unpractical that wastewater with dyes is trea...Almost all the studies both domestic and international using white rot fungus for dye wastewater treatment are performed under sterile conditions. However, it is obviously unpractical that wastewater with dyes is treated under sterile conditions. A feasible study was made for using white rot fungus Phanerochaete chrysosporium to degrade reactive brilliant red K-2BP dye under non-sterile conditions. The results showed that there was no decolorizing effect under non-sterile condition if white rot fungus was incubated under non-sterile condition, and the decolorization was always near to 0% during decolorizing test for 3 d; in the meantime, a lot of yeast funguses were found in liquid medium when white rot fungus was incubated under non-sterile conditions; however, if white rot fungus was incubated under sterile condition firstly, its decolorization was above 90% under non-sterile condition, which was similar to that of sterile condition. So we point out that the treating process for wastewater with dyes should be divided into two stages. The first stage is that white rot fungus should be incubated under sterile conditions, and the second stage is that reactive brilliant red K-2BP is decolorized under non-sterile conditions. The method not only save the operation cost which decolorizing reactive brilliant red K-2BP under sterile condition, but also provide the feasibility for using white rot fungus to degrade wastewater with dyes under non-sterile conditions.展开更多
Reactive Red 195, which is an azoic anionic dye characterized by the presence of five sulfonic groups and one azoic group, is efficiently removed using chitosan. The increasing chitosan dose had a dramatic positive im...Reactive Red 195, which is an azoic anionic dye characterized by the presence of five sulfonic groups and one azoic group, is efficiently removed using chitosan. The increasing chitosan dose had a dramatic positive impact on the achieved color removal, there was approximately a linear relationship between chitosan dose and color removal of dye before color removal reach maximum. Also, the increase of dye concentration led to the increase of chitosan dosage in order to get the same color remova l.92 mg/L of chitosan dosage was sufficient to achieve complete remove of dye at initial concentration of dye at 200 mg/L. For the higher concentrations of dye, high dosages were necessary to reach complete color removal. On the other hand, the use of adsorption interferents(Fe^2+ , Na^+ , HCO3^- and others) can be interesting, addition of ions had effect on the color removal of Reactive Red 195. Comparing with blank, addition of chemical species approximately decreased the color removal except Na^+ and combination of Fe^2+ + HCO3^- . However, comparing with Fe^2+ alone and HCO3^- alone, combination of Fe^2+ + HCO3^- increased the color removal.展开更多
The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme ofPhanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor ...The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme ofPhanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor (MBR) was also investigated by scanning electron microscope (SEM). The variation of peroxidase isoenzyme and the decolorization rate in the continuous MBR were evaluated. The results showed that the 100 mg/L RBR X-3B could stimulate the production of the peroxidase isoenzyme in the shaking-flask culture. In addition, two new peroxidase isoenzyme bands with relative mobility (Rf) value of 0.27 and 0.28 appeared, but the activity was lower than the blank control of 11 d. In the continuous MBR, the system worked stably during the first 60 d, the main peroxidase isoenzyme bands existed and three new bands with Rf value of 0.10, 0.27, and 0.28 appeared. Meanwhile, the biofilm grew well and the average decolorization rate could reach 90.6%. But the bands of peroxidase isoenzyme decreased rapidly at day 65, only two bands with Rf value 0.24 and 0.26 existed, and the decolorization rate decreased to 78.3%. Therefore, 5 bottles of P. chrysosporium mycelial pellet were added into the MBR, and then the activity of the peroxidase isoenzyme and the decolorization rate had a slight recovery. Finally, the decolorization rate finally decreased to 75.2%. These results contribute to a comprehensive understanding of the variation of peroxidase isoenzyme and biofilm in continuous MBR by white rot fungi.展开更多
Bentonite is a kind of natural clay with good exchanging ability. By exchanging its interlamellar cations with various soluble cations, such as quaternary ammonium cations and inorganic metal ions, the properties of n...Bentonite is a kind of natural clay with good exchanging ability. By exchanging its interlamellar cations with various soluble cations, such as quaternary ammonium cations and inorganic metal ions, the properties of natural bentonite can be greatly improved. In this study, hexadecyltrimethylammonium bromide (HDTMA), CaCl2, MgCl2, FeCl3, AlCl3 were used as organic and inorganic pillared materials respectively to produce several kinds of Ca-, Mg-, Fe-, Al-organo pillared bentonites. Sorption of reactive red X-3B on them was studied to determine their potential application as sorbents in wastewater treatment. The results showed that these pillared bentonites had much improved sorption properties, and that the dye solutions’ pH value had some effect on the performance of these inorgano-organo pillared bentonites. Isotherms of reactive X-3B on these pillared bentonites sug-gested a Langmuir-type sorption mechanism.展开更多
The adsorption of reactive red 239 (RR239) dye onto chitosan 8B was studied in aqueous solution at various pHs, initial dye concentrations, ionic strengths and temperatures, respectively. The adsorption of dye onto ch...The adsorption of reactive red 239 (RR239) dye onto chitosan 8B was studied in aqueous solution at various pHs, initial dye concentrations, ionic strengths and temperatures, respectively. The adsorption of dye onto chitosan 8B was confirmed by diffuse reflectance electronic absorption spectra. The adsorption of RR239 onto chitosan 8B was greatly influenced by solution pHs, initial dye concentrations, ionic strengths and temperatures. The kinetics and mechanism of dye adsorption process were analyzed by pseudo first-, second-order, Elovich and intraparticle diffusion kinetic models. The adsorption kinetics of RR239 dye followed a pseudo second-order model very well. The surface sorption and intraparticle diffusion mechanisms were involved in the actual sorption process. The equilibrium isotherm data were fitted well with the Langmuir model rather than the Freundlich, Temkin and Dubinin-Radushkevich models. The maximum dye adsorption onto chitosan 8B was estimated to be 163.93 μmol/g at 45°C. The activation energy (Ea) was obtained to be 23.30 kJ/mol. The computed thermodynamic parameters such as ΔG, ΔH, ΔS, ΔG, ΔH and ΔS confirmed that the adsorption of RR239 dye onto chitosan 8B was a spontaneous endothermic physisorption process. Desorption test was carried out in NaOH solution (pH 12.5) and the chitosan flakes could be reused.展开更多
Dyes often include toxic,carcinogenic compounds and are harmful to humans' health.Therefore,removal of dyes from textile industry wastewater is essential.The present study aimed to evaluate the efficiency of the c...Dyes often include toxic,carcinogenic compounds and are harmful to humans' health.Therefore,removal of dyes from textile industry wastewater is essential.The present study aimed to evaluate the efficiency of the combination of zero valent iron(ZVI) powder and multi-walled carbon nanotubes(MWCNTs) in the removal of Reactive Red 198(RR198) dye from aqueous solution.This applied research was performed in a batch system in the laboratory scale.This study investigated the effect of various factors influencing dye removal,including contact time,p H,adsorbent dose,iron powder dose,initial dye concentration,and temperature.The equilibrium adsorption data were analyzed using three common adsorption models:Langmuir,Freundlich and Temkin.Besides,kinetic and thermodynamic parameters were used to establish the adsorption mechanism.The results showed,in pH =3,contact time = 100 min,ZVI dose = 5000 mg·L^(-1),and MWCNTs dose = 600 mg·L^(-1)in 100 mg·L^(-1)dye concentration,the adsorption efficiency increased to 99.16%.Also,adsorption kinetics was best described by the pseudo-second-order model.Equilibrium data fitted well with the Freundlich isotherm(R2= 0.99).The negative values of ΔG0and the positive value of ΔH0(91.76) indicate that the RR198 adsorption process is spontaneous and endothermic.According to the results,the combination of MWCNTs and ZVI was highly efficient in the removal of azo dyes.展开更多
染料是一种结构复杂、具有剧毒的有机化合物,对人体毒害作用大。本研究制备了一种基于活性赤泥(activated red mud,ARM)和聚合吡咯(polypyrrole,PPy)的吸附剂,并应用于水溶液中染料的吸附脱除。通过吸附实验研究了溶液pH、吸附剂种类、...染料是一种结构复杂、具有剧毒的有机化合物,对人体毒害作用大。本研究制备了一种基于活性赤泥(activated red mud,ARM)和聚合吡咯(polypyrrole,PPy)的吸附剂,并应用于水溶液中染料的吸附脱除。通过吸附实验研究了溶液pH、吸附剂种类、吸附时间、添加量和染料初始浓度对吸附过程的影响,并对ARM-PPy复合材料进行分析表征,阐明了活性黄染料-145(RYD-145)的吸附作用机制。RYD-145在ARM-PPy上的吸附过程与三种吸附等温线关联极好(R2>0.99),其中Langmuir吸附最合适。根据Langmuir吸附模型的拟合结果,RYD-145的最大吸附量是442.5 mg/g。热力学研究表明,RYD-145分子在ARM-PPy的吸附过程中,焓变(ΔH)和熵变(ΔS)均增加,这表明该过程为吸热过程,且表面RYD-145分子排列具有随机性。研究表明,ARM-PPy具有高效处理阴离子和阳离子染料污染废水的能力,且具有较好的成本效益。展开更多
Ectonucleotidases are enzymes involved in nucleotides metabolism. The amount of circulating nucleotides may modulate distinct pathophysiological processes in the vasculature, including platelet aggregation and vascula...Ectonucleotidases are enzymes involved in nucleotides metabolism. The amount of circulating nucleotides may modulate distinct pathophysiological processes in the vasculature, including platelet aggregation and vascular tone. Onion (<em>Allium cepa</em> L.) phenolic compounds modulate enzymatic activity. The present study evaluated the total phenolic content of red onion methanolic extract, its antioxidant capacity, and its ability to interfere in nucleotides hydrolyses in rat serum. Total phenolic content was determined with the Folin-Ciocalteau reagent using gallic acid as a standard, while total flavonoid content was obtained through the aluminum chloride colorimetric method with quercetin as a standard. Antioxidant capacity was evaluated from the ability of the extract to scavenge ABTS<strong>·</strong>+ and DPPH<strong>·</strong> radicals. ATP, ADP, AMP, and p-Nph-5'-TMP hydrolyses were colorimetrically determined in response to different onion extract concentrations (0, 125, 250, 500, or 1000 μg/mL). Phenolic content of the extract was 54.35 mg GAE 100 g<sup>-1</sup> sample, while flavonoid content was 7.22 mg quercetin g<sup>-1</sup> sample. The IC50 value for ABTS<strong>·</strong>+ was 374.13 ± 7.52 μg/mL, while it was 440.29 ± 15.17 μg/mL for DPPH<strong>·</strong>. Red onion extract increased ADP and p-Nph-5'-TMP hydrolysis. The results confirmed that red onion contains high content of antioxidant, mainly flavonoids, and high antioxidant capacity. Additionally, biochemical studies suggest that the increased ADP breakdown may be important to regulate vascular processes. As it occurs for other enzymes, the antioxidant capacity of onion extract may neutralize reactive oxygen species (ROS) formation and favor ectonucleotidase activity and the hydrolysis of ADP, a major platelet agonist.展开更多
文摘Ecological toxicity of reactive X\|3B red dye and cadmium in both their single form and their combined form on wheat was studied using the experimental method of seed and root exposure. The single factor exposure indicated that the inhibitory rate of wheat root elongation was significantly increased with the increase in the concentration of the dye in the cultural solution, although seed germination of wheat was not sensitive to the dye. The toxicity of cadmium was greatly higher than that of the dye, but low concentration cadmium (<40 mg/L) could promote the germination of wheat seed. Interactive effects of the dye and cadmium on wheat were complicated. There was no significant correlation between the inhibitory rate of seed germination and the concentrations of the dye and cadmium. Low concentration cadmium could strengthen the toxicity of the dye acting on root elongation. On the contrary, high concentration cadmium could weaken the toxicity of the dye acting on root elongation.
文摘以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactive Red241模拟染料废水的脱色率可达到86%以上;在p H=7,U=14V、I=3.2A、t=25min的条件下,C.I.Disperse Blue 56模拟染料废水的脱色率可达到79以上%。
基金This work is supported by the National Key Basic Research Program (2006CB701406);Natural Science Foundation of China for Youth (40403003) ;Key Project of the Natural Science Foundation of China (40332020), and is a contribution to IGCP 463.
文摘One of the new directions in the field of Cretaceous research is to elucidate the mechanism of the sedimentary transition from the Cretaceous black shales to oceanic red beds. A chemical sequential extraction method was applied to these two types of rocks from southern Tibet to investigate the burial records of reactive iron. Results indicate that carbonate-associated iron and pyrite are relatively enriched in the black shales, but depleted or absent in red beds. The main feature of the reactive iron in the red beds is relative enrichment of iron oxides (largely hematite), which occurred during syn-depostion or early diagenesis. The ratio between iron oxides and the total iron indicates an oxygen-enriched environment for red bed deposition. A comparison between the reactive iron burial records and proxies of paleo-productivity suggests that paleo-productivity decreases when the ratio between iron oxides and the total iron increases in the red beds. This phenomenon could imply that the relationship between marine redox and productivity might be one of the reasons for the sedimentary transition from Cretaceous black shale to oceanic red bed deposition.
基金supported by the Major Projects on Control and Rectification of Water Body Pollution (Water Special Project) (No.2009ZX07010-001,2008ZX07526-001)the National Basic Research Program (973) of China (No.2008CB418201)
文摘A comparative study of treatment of simulated wastewater containing Reactive Red 195 using zero-valent iron/activated carbon (ZVI/AC), microwave discharge electrodeless lamp/sodium hypochlorite (MDEL/NaCIO) and the combination of ZVI/AC- MDEL/NaCIO was conducted. The preliminary results showed the two steps method of ZVI/AC-MDEL/NaCIO had much higher degradation efficiency than both single steps. The final color removal percentage was nearly up to 100% and the chemical oxygen demand reduction percentage was up to approximately 82%. The effects of operational parameters, including initial pH value of simulated wastewater, ZVI/AC ratio and particle size of ZVI were also investigated. In addition, from the discussion of synergistic effect between ZVI/AC and MEDL/NaCIO, we found that in the ZVI/AC-MEDL/NaCIO process, ZVI/AC could break the azo bond firstly and then MEDLfNaCIO degraded the aromatic amine products effectively. Reversing the order would reduce the degradation efficiency.
基金supported by the Natural Science Foundation of China for Youth(40403003)National Key Basic Research Program(2006CB701406)Key Project of Natural Science Foundation of China(40332020),and is a contribution to IGCP 463/555.
文摘The mechanism of sedimentary transition from the Cretaceous black shales to the oceanic red beds is a new and important direction of Cretaceous research. Chemical sequential extraction is applied to study the burial records of reactive phosphorus in the black shale of the Gyabula Formation and oceanic red beds of the Chuangde Formation, Southern Tibet. Results indicate that the principal reactive phosphorus species is the authigenic and carbonate-associated phosphorus (CAP) in the Gyabula Formation and iron oxides-associated phosphorus (FeP) in the Chuangde Formation which accounts for more than half of their own total phosphorus content. While the authigenic and carbonate-associated phosphorus (CAP) is almost equal in the two Formations; the iron oxidesassociated phosphorus is about 1.6 times higher in the Chuangde Formation than that in the Gyabula Formation resulting in a higher content of the total phosphorus in the Chuangde Formation. According to the observations on the marine phosphorus cyde in Modern Ocean, it is found that preferential burial and regeneration of reactive phosphorus corresponds to highly oxic and reducing conditions, respectively, leading to the different distribution of phosphorus in these two distinct type of marine sediments. It is the redox-sensitive behavior of phosphorus cycle to the different redox conditions in the ocean and the controlling effects of phosphorus to the marine production that stimulate the local sedimentary transition from the Cretaceous black shale to the oceanic red beds.
基金The National Natural Science Foundation of China (No. 50478010) and the Chinese Postdoctoral Science Foundation (No.20040350022)
文摘Almost all the studies both domestic and international using white rot fungus for dye wastewater treatment are performed under sterile conditions. However, it is obviously unpractical that wastewater with dyes is treated under sterile conditions. A feasible study was made for using white rot fungus Phanerochaete chrysosporium to degrade reactive brilliant red K-2BP dye under non-sterile conditions. The results showed that there was no decolorizing effect under non-sterile condition if white rot fungus was incubated under non-sterile condition, and the decolorization was always near to 0% during decolorizing test for 3 d; in the meantime, a lot of yeast funguses were found in liquid medium when white rot fungus was incubated under non-sterile conditions; however, if white rot fungus was incubated under sterile condition firstly, its decolorization was above 90% under non-sterile condition, which was similar to that of sterile condition. So we point out that the treating process for wastewater with dyes should be divided into two stages. The first stage is that white rot fungus should be incubated under sterile conditions, and the second stage is that reactive brilliant red K-2BP is decolorized under non-sterile conditions. The method not only save the operation cost which decolorizing reactive brilliant red K-2BP under sterile condition, but also provide the feasibility for using white rot fungus to degrade wastewater with dyes under non-sterile conditions.
基金The State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technologyin Zhejiang University of Technology
文摘Reactive Red 195, which is an azoic anionic dye characterized by the presence of five sulfonic groups and one azoic group, is efficiently removed using chitosan. The increasing chitosan dose had a dramatic positive impact on the achieved color removal, there was approximately a linear relationship between chitosan dose and color removal of dye before color removal reach maximum. Also, the increase of dye concentration led to the increase of chitosan dosage in order to get the same color remova l.92 mg/L of chitosan dosage was sufficient to achieve complete remove of dye at initial concentration of dye at 200 mg/L. For the higher concentrations of dye, high dosages were necessary to reach complete color removal. On the other hand, the use of adsorption interferents(Fe^2+ , Na^+ , HCO3^- and others) can be interesting, addition of ions had effect on the color removal of Reactive Red 195. Comparing with blank, addition of chemical species approximately decreased the color removal except Na^+ and combination of Fe^2+ + HCO3^- . However, comparing with Fe^2+ alone and HCO3^- alone, combination of Fe^2+ + HCO3^- increased the color removal.
基金supported by the National High Technology Research and Development Program (863) of China (No. 2006AA06Z331)the National Natural Science Foundation of China (No. 50278034)
文摘The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme ofPhanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor (MBR) was also investigated by scanning electron microscope (SEM). The variation of peroxidase isoenzyme and the decolorization rate in the continuous MBR were evaluated. The results showed that the 100 mg/L RBR X-3B could stimulate the production of the peroxidase isoenzyme in the shaking-flask culture. In addition, two new peroxidase isoenzyme bands with relative mobility (Rf) value of 0.27 and 0.28 appeared, but the activity was lower than the blank control of 11 d. In the continuous MBR, the system worked stably during the first 60 d, the main peroxidase isoenzyme bands existed and three new bands with Rf value of 0.10, 0.27, and 0.28 appeared. Meanwhile, the biofilm grew well and the average decolorization rate could reach 90.6%. But the bands of peroxidase isoenzyme decreased rapidly at day 65, only two bands with Rf value 0.24 and 0.26 existed, and the decolorization rate decreased to 78.3%. Therefore, 5 bottles of P. chrysosporium mycelial pellet were added into the MBR, and then the activity of the peroxidase isoenzyme and the decolorization rate had a slight recovery. Finally, the decolorization rate finally decreased to 75.2%. These results contribute to a comprehensive understanding of the variation of peroxidase isoenzyme and biofilm in continuous MBR by white rot fungi.
基金Project supported by the Scientific Research Foundation for Re-turned Overseas Scholars and the National Natural Science Founda-tion of China (No. 40576080)
文摘Bentonite is a kind of natural clay with good exchanging ability. By exchanging its interlamellar cations with various soluble cations, such as quaternary ammonium cations and inorganic metal ions, the properties of natural bentonite can be greatly improved. In this study, hexadecyltrimethylammonium bromide (HDTMA), CaCl2, MgCl2, FeCl3, AlCl3 were used as organic and inorganic pillared materials respectively to produce several kinds of Ca-, Mg-, Fe-, Al-organo pillared bentonites. Sorption of reactive red X-3B on them was studied to determine their potential application as sorbents in wastewater treatment. The results showed that these pillared bentonites had much improved sorption properties, and that the dye solutions’ pH value had some effect on the performance of these inorgano-organo pillared bentonites. Isotherms of reactive X-3B on these pillared bentonites sug-gested a Langmuir-type sorption mechanism.
文摘The adsorption of reactive red 239 (RR239) dye onto chitosan 8B was studied in aqueous solution at various pHs, initial dye concentrations, ionic strengths and temperatures, respectively. The adsorption of dye onto chitosan 8B was confirmed by diffuse reflectance electronic absorption spectra. The adsorption of RR239 onto chitosan 8B was greatly influenced by solution pHs, initial dye concentrations, ionic strengths and temperatures. The kinetics and mechanism of dye adsorption process were analyzed by pseudo first-, second-order, Elovich and intraparticle diffusion kinetic models. The adsorption kinetics of RR239 dye followed a pseudo second-order model very well. The surface sorption and intraparticle diffusion mechanisms were involved in the actual sorption process. The equilibrium isotherm data were fitted well with the Langmuir model rather than the Freundlich, Temkin and Dubinin-Radushkevich models. The maximum dye adsorption onto chitosan 8B was estimated to be 163.93 μmol/g at 45°C. The activation energy (Ea) was obtained to be 23.30 kJ/mol. The computed thermodynamic parameters such as ΔG, ΔH, ΔS, ΔG, ΔH and ΔS confirmed that the adsorption of RR239 dye onto chitosan 8B was a spontaneous endothermic physisorption process. Desorption test was carried out in NaOH solution (pH 12.5) and the chitosan flakes could be reused.
文摘Dyes often include toxic,carcinogenic compounds and are harmful to humans' health.Therefore,removal of dyes from textile industry wastewater is essential.The present study aimed to evaluate the efficiency of the combination of zero valent iron(ZVI) powder and multi-walled carbon nanotubes(MWCNTs) in the removal of Reactive Red 198(RR198) dye from aqueous solution.This applied research was performed in a batch system in the laboratory scale.This study investigated the effect of various factors influencing dye removal,including contact time,p H,adsorbent dose,iron powder dose,initial dye concentration,and temperature.The equilibrium adsorption data were analyzed using three common adsorption models:Langmuir,Freundlich and Temkin.Besides,kinetic and thermodynamic parameters were used to establish the adsorption mechanism.The results showed,in pH =3,contact time = 100 min,ZVI dose = 5000 mg·L^(-1),and MWCNTs dose = 600 mg·L^(-1)in 100 mg·L^(-1)dye concentration,the adsorption efficiency increased to 99.16%.Also,adsorption kinetics was best described by the pseudo-second-order model.Equilibrium data fitted well with the Freundlich isotherm(R2= 0.99).The negative values of ΔG0and the positive value of ΔH0(91.76) indicate that the RR198 adsorption process is spontaneous and endothermic.According to the results,the combination of MWCNTs and ZVI was highly efficient in the removal of azo dyes.
文摘染料是一种结构复杂、具有剧毒的有机化合物,对人体毒害作用大。本研究制备了一种基于活性赤泥(activated red mud,ARM)和聚合吡咯(polypyrrole,PPy)的吸附剂,并应用于水溶液中染料的吸附脱除。通过吸附实验研究了溶液pH、吸附剂种类、吸附时间、添加量和染料初始浓度对吸附过程的影响,并对ARM-PPy复合材料进行分析表征,阐明了活性黄染料-145(RYD-145)的吸附作用机制。RYD-145在ARM-PPy上的吸附过程与三种吸附等温线关联极好(R2>0.99),其中Langmuir吸附最合适。根据Langmuir吸附模型的拟合结果,RYD-145的最大吸附量是442.5 mg/g。热力学研究表明,RYD-145分子在ARM-PPy的吸附过程中,焓变(ΔH)和熵变(ΔS)均增加,这表明该过程为吸热过程,且表面RYD-145分子排列具有随机性。研究表明,ARM-PPy具有高效处理阴离子和阳离子染料污染废水的能力,且具有较好的成本效益。
文摘Ectonucleotidases are enzymes involved in nucleotides metabolism. The amount of circulating nucleotides may modulate distinct pathophysiological processes in the vasculature, including platelet aggregation and vascular tone. Onion (<em>Allium cepa</em> L.) phenolic compounds modulate enzymatic activity. The present study evaluated the total phenolic content of red onion methanolic extract, its antioxidant capacity, and its ability to interfere in nucleotides hydrolyses in rat serum. Total phenolic content was determined with the Folin-Ciocalteau reagent using gallic acid as a standard, while total flavonoid content was obtained through the aluminum chloride colorimetric method with quercetin as a standard. Antioxidant capacity was evaluated from the ability of the extract to scavenge ABTS<strong>·</strong>+ and DPPH<strong>·</strong> radicals. ATP, ADP, AMP, and p-Nph-5'-TMP hydrolyses were colorimetrically determined in response to different onion extract concentrations (0, 125, 250, 500, or 1000 μg/mL). Phenolic content of the extract was 54.35 mg GAE 100 g<sup>-1</sup> sample, while flavonoid content was 7.22 mg quercetin g<sup>-1</sup> sample. The IC50 value for ABTS<strong>·</strong>+ was 374.13 ± 7.52 μg/mL, while it was 440.29 ± 15.17 μg/mL for DPPH<strong>·</strong>. Red onion extract increased ADP and p-Nph-5'-TMP hydrolysis. The results confirmed that red onion contains high content of antioxidant, mainly flavonoids, and high antioxidant capacity. Additionally, biochemical studies suggest that the increased ADP breakdown may be important to regulate vascular processes. As it occurs for other enzymes, the antioxidant capacity of onion extract may neutralize reactive oxygen species (ROS) formation and favor ectonucleotidase activity and the hydrolysis of ADP, a major platelet agonist.