Adriamycin resistant cells were obtained from low dotage treated BABL/c mice Inoculated with S-180 cells. Resistance of these cells for adriamycin was 66-fold more than their parental cells. The resistance for a typic...Adriamycin resistant cells were obtained from low dotage treated BABL/c mice Inoculated with S-180 cells. Resistance of these cells for adriamycin was 66-fold more than their parental cells. The resistance for a typical DNA topoisomerase Ⅱ inhibitor VP16 (Etopcaide) was increased 9 times. Overexpression of multidrug resistant gene (MDR gene) products, P-glycoproteins (P-1 70), was also demonstrated by immunohistochemistry. Furthermore, the ability of the resistant cells to reduce net cellular drug accumulation measured by flow fluorescence cytometry was 89-fold higher than their parental cells. These results support the hypothesis that the resistance of S-180R cells to adriamycin was mainly due to the overexpression of P-glycoproteins. The S-180R cells will be useful to select drugs or some other therapeutic strategies to overcome multidrug resistance in vivo.展开更多
[Objective]The resistance to Tetranychus cinnabarinus(Boisduva)of 84 cassava gemplasms resources from the nursery of Guangxi Institute of Southern Tropical Agricultural Sciences was eraluated.[Method] Thuree random le...[Objective]The resistance to Tetranychus cinnabarinus(Boisduva)of 84 cassava gemplasms resources from the nursery of Guangxi Institute of Southern Tropical Agricultural Sciences was eraluated.[Method] Thuree random leaves were taken from Casiva plants separately from the upper,middle and lower parts,9 leaves from each plant to investigate the damage degree and calculate the mite damage index.[Result]The Tesults showed that among the 84 cassava gemplasms,6 were resistant(R),39 moderately resistant(MR),31 sensitive(S),and 8 highly sensitive(HS)Six germplasms such as Guiken 09-11 and SC5 could be used as breeding materials with resistance,and the vanietics with large planting area in Guangxi such as GR3,SC201,GR911,SC205 and SC11 were all moderately rsistant.The resistance of edible Cassava vanieties was poor,among which GR891 and South Asia Cassava No.1 have the lowest resistance.[Conclusion]No conelation was found between mite damage index of Ca8-sava germplasm and plant type,number of branches,lobe shape,leaf length,leaf width and leaf thicknes.展开更多
文摘Adriamycin resistant cells were obtained from low dotage treated BABL/c mice Inoculated with S-180 cells. Resistance of these cells for adriamycin was 66-fold more than their parental cells. The resistance for a typical DNA topoisomerase Ⅱ inhibitor VP16 (Etopcaide) was increased 9 times. Overexpression of multidrug resistant gene (MDR gene) products, P-glycoproteins (P-1 70), was also demonstrated by immunohistochemistry. Furthermore, the ability of the resistant cells to reduce net cellular drug accumulation measured by flow fluorescence cytometry was 89-fold higher than their parental cells. These results support the hypothesis that the resistance of S-180R cells to adriamycin was mainly due to the overexpression of P-glycoproteins. The S-180R cells will be useful to select drugs or some other therapeutic strategies to overcome multidrug resistance in vivo.
基金Sponsored by Major Special Project of Guangxi Provincial Science and Technology Program。
文摘[Objective]The resistance to Tetranychus cinnabarinus(Boisduva)of 84 cassava gemplasms resources from the nursery of Guangxi Institute of Southern Tropical Agricultural Sciences was eraluated.[Method] Thuree random leaves were taken from Casiva plants separately from the upper,middle and lower parts,9 leaves from each plant to investigate the damage degree and calculate the mite damage index.[Result]The Tesults showed that among the 84 cassava gemplasms,6 were resistant(R),39 moderately resistant(MR),31 sensitive(S),and 8 highly sensitive(HS)Six germplasms such as Guiken 09-11 and SC5 could be used as breeding materials with resistance,and the vanietics with large planting area in Guangxi such as GR3,SC201,GR911,SC205 and SC11 were all moderately rsistant.The resistance of edible Cassava vanieties was poor,among which GR891 and South Asia Cassava No.1 have the lowest resistance.[Conclusion]No conelation was found between mite damage index of Ca8-sava germplasm and plant type,number of branches,lobe shape,leaf length,leaf width and leaf thicknes.
