Fox is an important economic fur animal with a very promising prospect. The fox mitochondrial COl gene-specific primers and probes were used to opti- mize and develop a real time fluorescence PCR system for rapid dete...Fox is an important economic fur animal with a very promising prospect. The fox mitochondrial COl gene-specific primers and probes were used to opti- mize and develop a real time fluorescence PCR system for rapid detection of fox-derived components. The results showed that the real time fluorescence PCR gave a very high sensitivity, with the detection limit as low as 12 fg DNA, and it did not present any cross reaction with other common species like pig, cow, sheep, dog, soybean and maize. It could be concluded that this method can be used as an effective method for detecting the fox-derived components in food and feedstuff, and can also be applied for distinguishing the fox fur from adulterants.展开更多
基金Supported by Program of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China(2011IK011)
文摘Fox is an important economic fur animal with a very promising prospect. The fox mitochondrial COl gene-specific primers and probes were used to opti- mize and develop a real time fluorescence PCR system for rapid detection of fox-derived components. The results showed that the real time fluorescence PCR gave a very high sensitivity, with the detection limit as low as 12 fg DNA, and it did not present any cross reaction with other common species like pig, cow, sheep, dog, soybean and maize. It could be concluded that this method can be used as an effective method for detecting the fox-derived components in food and feedstuff, and can also be applied for distinguishing the fox fur from adulterants.
