Cyber Resilience through Real-Time Threat Analysis in Information Security

This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends that they are not the same. The concept of cyber security is explored, which goes beyond protecting information resources to include a wider variety of assets, including people [1]. Protecting information assets is the main goal of traditional information security, with consideration to the human element and how people fit into the security process. On the other hand, cyber security adds a new level of complexity, as people might unintentionally contribute to or become targets of cyberattacks. This aspect presents moral questions since it is becoming more widely accepted that society has a duty to protect weaker members of society, including children [1]. The study emphasizes how important cyber security is on a larger scale, with many countries creating plans and laws to counteract cyberattacks. Nevertheless, a lot of these sources frequently neglect to define the differences or the relationship between information security and cyber security [1]. The paper focus on differentiating between cybersecurity and information security on a larger scale. The study also highlights other areas of cybersecurity which includes defending people, social norms, and vital infrastructure from threats that arise from online in addition to information and technology protection. It contends that ethical issues and the human factor are becoming more and more important in protecting assets in the digital age, and that cyber security is a paradigm shift in this regard [1].

Design, Analysis and Real-time Implementation of Networked Predictive Control Systems

这份报纸用预兆的控制策略涉及设计，分析和联网的控制系统的即时实现。联网的控制系统的特征的分析被详细说明，它证明一个联网的控制系统与常规控制系统不同。完成靠近环的联网的控制系统的需要的性能，联网的预兆的控制计划被介绍。设计，稳定性分析和联网的预兆的控制系统的即时实现被学习。它被联网的预兆的控制计划能补偿随机的网络通讯延期和数据退学学生的模拟和实际实验说明，完成需要的控制性能并且有好靠近环的稳定性。

A Mathematical Model of Real-Time Simulation and the Convergence Analysis on Real-Time Runge-Kutta Algorithms

In this paper, a mathematical model of real-time simulation is given, and the problem of convergence on real-time Runge-Kutta algorithms is analysed. At last a theorem on the relation between the order of compensation and the convergent order of real-time algorithm is proved.

Integrated Real-Time Big Data Stream Sentiment Analysis Service

Opinion (sentiment) analysis on big data streams from the constantly generated text streams on social media networks to hundreds of millions of online consumer reviews provides many organizations in every field with opportunities to discover valuable intelligence from the massive user generated text streams. However, the traditional content analysis frameworks are inefficient to handle the unprecedentedly big volume of unstructured text streams and the complexity of text analysis tasks for the real time opinion analysis on the big data streams. In this paper, we propose a parallel real time sentiment analysis system: Social Media Data Stream Sentiment Analysis Service (SMDSSAS) that performs multiple phases of sentiment analysis of social media text streams effectively in real time with two fully analytic opinion mining models to combat the scale of text data streams and the complexity of sentiment analysis processing on unstructured text streams. We propose two aspect based opinion mining models: Deterministic and Probabilistic sentiment models for a real time sentiment analysis on the user given topic related data streams. Experiments on the social media Twitter stream traffic captured during the pre-election weeks of the 2016 Presidential election for real-time analysis of public opinions toward two presidential candidates showed that the proposed system was able to predict correctly Donald Trump as the winner of the 2016 Presidential election. The cross validation results showed that the proposed sentiment models with the real-time streaming components in our proposed framework delivered effectively the analysis of the opinions on two presidential candidates with average 81% accuracy for the Deterministic model and 80% for the Probabilistic model, which are 1% - 22% improvements from the results of the existing literature.

Electricity Price Influence Factors Analysis Using Stochastic Matrix for Real-Time Electricity Price Forecasting

Real-time electricity price( RTEP) influence factor extraction is essential to forecasting accurate power system electricity prices. At present,new electricity price forecasting models have been studied to improve predictive accuracy,ignoring the extraction and analysis of RTEP influence factors. In this study,a correlation analysis method is proposed based on stochastic matrix theory.Firstly, an augmented matrix is formulated, including RTEP influence factor data and RTEP state data. Secondly, data correlation analysis results are obtained given the statistical characteristics of source data based on stochastic matrix theory.Mean spectral radius( MSR) is used as the measure of correlativity.Finally,the proposed method is evaluated in New England electricity markets and compared with the BP neural network forecasting method. Experimental results show that the extracted index system comprehensively generalizes RTEP influence factors,which play a significant role in improving RTEP forecasting accuracy.

Real-Time Computer Analytical Application in Pressure Transient Analysis of Petroleum Reservoirs

Today with certainty, the petroleum industry is fostering sanguinely the fields’ development programs for the optimization of reservoir characterization through worth-full appliances of computer analysis techniques. The time element is of prime importance for optimistic petroleum development projects. Therefore, the frontline of “Real-time Analysis” is added into the applications of computer solving techniques for achieving and sketching up the real-time cost effectiveness in analyzing field development programs. It focuses on the phases of real-time well test data acquisition system, real-time secure access to the well test data either on field or in office and real-time data interpretation unit. This interface will yield the productive results for the field of reservoir’s pressure transient analysis and wells’ systems analysis by following the up-to-date preferred, accurate and effective well test analytical principles with modern real-time computer applications and techniques. It also lays emphasis for the comfort and reliability of data in creating best interpersonal working modes within a reputable and esteemed petroleum development organization.

基因Ⅰ型草鱼呼肠孤病毒TaqMan Real-Time PCR检测方法的建立及应用

为了建立和应用可检测基因I型草鱼呼肠孤病毒（GCRV）的荧光定量检测方法，实验根据基因I型GCRV的s6保守序列，分别设计扩增目的条带661bp普通引物（P1、P2）、扩增目的条带159bp荧光定量引物（F1、F2）和一条探针；同时构建含有目的片段的PVAX1-S6作为标准质粒，10倍梯度稀释构建质粒拷贝数与Ct值的标准曲线（Y=-3．389X＋38．076）。结果表明，此方法在3．9×10^7～3．9×10^1拷贝／uL之间相关性较好，R2为0．992，最小检测量为4拷贝／uL；且与基因Ⅱ型、Ⅲ型GCRV以及其他病原核酸无交叉反应。运用该方法检测16份草鱼出血病疑似样品，阳性结果为4份；而普通PCR检测仅2份显示阳性。本研究建立了针对基因I型GCRV的荧光定量检测方法，具有高效、特异、灵敏、可重复性强的优点，适合于目前基因I型GCRV的临床快速检测和病毒定量分析。

AllGlo探针Real-time RT-PCR检测发热伴血小板减少综合征病毒

目的建立基于AllGlo探针Real—time RT—PCR技术检测发热伴血小板减少综合征病毒（SFTSV）的方法。方法根据SFTSV基因组S片段的相对保守序列设计引物和AllGlo探针，建立和优化Real—time RT—PCR反应体系，对其敏感性、特异性和稳定性进行评价，并检测临床疑似SFTSV病例标本，与普通RT—PCR法、病毒分离法和抗体检测法进行比较。结果 所建立AllGlo探针Real—time RT—PCR法检测SFTSV核酸，标准曲线方程为Y=3．38x＋46．03（Y为ct值，z为核酸拷贝数log值），r2〉O．998，扩增效率为97．6％，Ct值变异系数（CV）均〈1．2％，检测限为1．00×10^3copy／mL。与汉坦病毒、H1N1、H3N2、登革病毒1～4型均无交叉。检测362份疑似病例标本，SFTSV核酸阳性检出率11．9％，高于普通RT-PCR法、病毒分离法和抗体检测法（P值均d0．05）。结论建立的AllGlo探针Real—time RT-PCR法，操作简单、快速，灵敏性、特异性较高，可用于SFTSV核酸检测。

急性脑梗死外周血中Circ-BBS9的表达水平及临床意义

目的:检测急性脑梗死(ACI)中环状RNA-BBS9的表达水平,探讨其作为ACI早期诊断潜在生物标志物的可能性。方法:随机选取2021年1月至12月收治的34例ACI患者作为病例组,41例非ACI患者作为对照组,收集一般资料并采血行生信分析,筛选出差异性最大的Circ RNA作为目的基因,分析两组一般资料和目的基因表达水平与ACI发生的相关性;运用ROC曲线分析目的基因诊断ACI的价值。结果:病例组的男性占比、年龄、高血压病史率、冠心病病史率、吸烟史占比、饮酒史占比、血压水平均高于对照组(P<0.05)。差异性最大的Circ RNA为Circ-BBS9。病例组的Circ-BBS9表达水平低于对照组(P<0.05)。性别、年龄、高血压患病史、冠心病患病史、吸烟史、饮酒史、血压水平以及Circ-BBS9表达均是ACI发生的相关影响因素(P<0.05),其中Circ-BBS9表达水平与ACI发生呈负相关(P<0.05),其他因素与ACI发生呈正相关(P<0.05)。Circ-BBS9具有诊断ACI的价值。结论:Circ-BBS9是急性脑梗死发病的保护因素,Circ-BBS9的表达水平是影响ACI发生的独立因素,外周血检测Circ-BBS9具有预测ACI发病的潜在价值。

血清E型沙眼衣原体的Real-time PCR定量分析

目的沙眼衣原体感染是最常见的性传播疾病,本文拟建立一种准确快速、标准化的感染动物组织衣原体载量检测体系。方法体外扩增感染用沙眼衣原体血清E型,克隆衣原体特异基因OMP1基因片段作为标准品,用Real time PCR法测定衣原体基因组拷贝数进行衣原体定量。结果 Real time PCR在OMP1基因片段200至2×108拷贝检测结果成线性,在模板中加入小鼠基因组未出现非特异扩增,同时未影响扩增效率。结论针对衣原体特异基因OMP1的实时定量PCR方法可以较为灵敏的特异的定量检测感染动物样本中的衣原体。

Real-time crash prediction on freeways using data mining and emerging techniques

Recent advances in intelligent transportation system allow traffic safety studies to extend from historic data-based analyses to real-time applications. The study presents a new method to predict crash likelihood with traffic data collected by discrete loop detectors as well as the web-crawl weather data. Matched case-control method and support vector machines （SVMs） technique were employed to identify the risk status. The adaptive synthetic over-sampling technique was applied to solve the imbalanced dataset issues. Random forest technique was applied to select the contributing factors and avoid the over-fitting issues. The results indicate that the SVMs classifier could successfully classify 76.32% of the crashes on the test dataset and 87.52% of the crashes on the overall dataset, which were relatively satisfactory compared with the results of the previous studies. Compared with the SVMs classifier without the data, the SVMs classifier with the web-crawl weather data increased the crash prediction accuracy by 1.32% and decreased the false alarm rate by 1.72%, showing the potential value of the massive web weather data. Mean impact value method was employed to evaluate the variable effects, and the results are identical with the results of most of previous studies. The emerging technique based on the discrete traffic data and web weather data proves to be more applicable on real- time safety management on freeways.

鸽微RNA病毒实时荧光定量RT-PCR检测方法的建立

旨在建立鸽微RNA病毒(pigeon megrivirus,PiMeV)实时荧光定量RT-PCR检测方法。本研究根据GenBank中PiMeVs序列特征设计特异性检测引物,从信鸽粪便中检测到PiMeV阳性(命名为PiMeV-CHN001株),并对其3 C基因进行核苷酸同源性比较和遗传进化分析,明确其基因特征后,设计特异性实时荧光定量RT-PCR检测(RT-qPCR)引物组,建立检测PiMeV的RT-qPCR方法。结果显示:PiMeV-CHN001株3 C基因全长为591 bp,编码197个氨基酸,和其他2株野鸽源PiMeV(MeV-B1株和MeV-B2株)核苷酸相似性分别为89.5%和92.0%。建立的检测PiMeV的RT-qPCR方法的标准曲线Y轴截距为37.93,斜率为-3.335,相关系数为1.00,扩增效率为99.4%。特异性强,仅PiMeV出现特异性扩增信号和特异性峰值[Tm值为(81.69±0.22)℃],对鸽源禽流感病毒(avian influenza virus,AIV)、鸽源禽I型副黏病毒(pigeon paramyxovirus type I,PPMV-1)、鸽输血传播病毒(pigeon torque teno virus,PTTV)、鸽腺病毒(pigeon adenovirus,PiAd)及鸽圆环病毒(pigeon circovirus,PiCV)检测均未见特异性扩增信号;敏感性优,最低检测限为54.0拷贝·μL^(-1);重复性好,批内和批间变异系数均低于1.5%。用建立的检测方法对42份信鸽粪便样品进行检测,发现2份阳性样品(阳性率为4.76%)。本研究首次证实我国大陆地区信鸽中存在PiMeV,丰富了PiMeV宿主谱信息;建立的RT-qPCR方法为后续开展PiMeV流行病学研究提供支撑。

一种基于联合规划的CAN-TSN网关实时性分析方法

控制器局域网络(Controller Area Network)CAN-TSN(Time Sensitive Networking,时间敏感网络)网关是下一代车载网络架构中关键业务数据端到端传输路径上的重要环节,其实时性直接影响跨异构网络系统传输报文的可调度性。针对CAN-TSN网关实时性分析,现有研究存在网关处理延时组成要素不全、缺乏对TSN网络的调度规划结果的联合分析等亟待解决的问题,导致利用现有研究获取的可调度集往往无法满足端到端的截止时间约束。针对上述问题,提出一种基于联合规划的CAN-TSN网关实时性分析方法。该方法基于现有CAN-TSN网关架构设计构建通用框架,分析网关处理延时组成,获取处理延时的参数化模型;联合TSN网络的规划调度结果,推导满足报文端到端可调度性的网关延时上界;搭建CAN-TSN网关原型系统,使用真实车载CAN消息集进行可调度率分析。实验结果表明,基于联合规划的CAN-TSN网关实时性分析方法,能够收紧真实环境中CAN消息集的可调度范围,使得可调度率收紧约6.7%,同时能够对网关实时性架构优化起指导作用。

Genome-wide analysis and identification of cytokinin oxidase/dehydrogenase(CKX) gene family in foxtail millet(Setaria italica)

Cytokinin oxidase/dehydrogenase(CKX; EC.1.5.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants.

干血斑中34种苯二氮卓类物质的实时直接分析-串联质谱快速筛查研究

该文通过优化仪器条件和前处理方法建立了实时直接分析-串联质谱快速筛查干血斑(DBS)中34种苯二氮卓类物质(BZDs)的方法。首先将50μL血液沉积在采血卡上形成DBS,DBS经溶剂充分提取后取上层溶液,使用DART 12Dip-It^(TM)模块进样5μL,在多反应监测模式下检测。结果表明:选用400℃作为载气加热器温度,以乙酸乙酯-水(体积比3∶1)混合溶剂提取DBS样品,所得响应强度较高且建立的筛查方法选择性良好,无延迟效应影响,除阿地唑仑、奥沙西泮和α-羟基三唑仑外,其他物质的检出限均在5~50 ng/mL。所建方法可在90.3%的阳性检材中成功筛选出BZDs物质,方法快速、简便、有效,适用于服药自杀等类案件中DBS检材的快速筛查检验。

Delay-dependent stability and added damping of SDOF real-time dynamic hybrid testing

It is well-recognized that a transfer system response delay that reduces the test stability inevitably exists in real-time dynamic hybrid testing （RTDHT）. This paper focuses on the delay-dependent stability and added damping of SDOF systems in RTDHT. The exponential delay term is transferred into a rational fraction by the Pad6 approximation, and the delay-dependent stability conditions and instability mechanism of SDOF RTDHT systems are investigated by the root locus technique. First, the stability conditions are discussed separately for the cases of stiffness, mass, and damping experimental substructure. The use of root locus plots shows that the added damping effect and instability mechanism for mass are different from those for stiffness. For the stiffness experimental substructure case, the instability results from the inherent mode because of an obvious negative damping effect of the delay. For the mass case, the delay introduces an equivalent positive damping into the inherent mode, and instability occurs at an added high frequency mode. Then, the compound stability condition is investigated for a general case and the results show that the mass ratio may have both upper and lower limits to remain stable. Finally, a high-emulational virtual shaking table model is built to validate the stability conclusions.

Detection of Lactobacillus acidophilus in Fermented Material by Real-time Fluorescent Quantitative PCR

The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.

基于ADS-B和WEB的雷达精度分析系统

方位角、俯仰角、距离三维精度是三坐标雷达的重要技术指标,通常使用动态标校的方式进行标定;为解决动态标校试验周期长、成本高、难度大、在使用维护阶段难以实施等问题,文章介绍了一种基于广播式自动相关监视系统(ADS-B)的雷达精度准实时分析系统;文章从ADS-B数据率、位置精度、最大可接收距离等多个方面探讨了民航航迹用于雷达动态精度分析的可行性;采用Django框架设计、实现了WEB架构的雷达精度准实时分析系统;并使用高精度雷达对系统进行了验证、标定、系统误差补偿;最后,系统在某中远程雷达上进行多个批次测试,结果表明该方法有效且可行。