Quantity of bed load is an important physical parameter in sediment transport research. Aiming at the difficulties in the bed load measurement, this paper develops a bottom-mounted monitor to measure the bed load tran...Quantity of bed load is an important physical parameter in sediment transport research. Aiming at the difficulties in the bed load measurement, this paper develops a bottom-mounted monitor to measure the bed load transport rate by adopting the sedimentation pit method and resolving such key problems as weighing and desilting, which can achieve long-time, all-weather and real-time telemeasurement of the bed load transport rate of plain rivers, estuaries and coasts. Both laboratory and field tests show that this monitor is reasonable in design, stable in properties and convenient in measurement, and it can be used to monitor the bed load transport rate in practical projects.展开更多
In this paper, a novel scheduling mechanism is proposed to handle the real-time overload problem by maximizing the cumulative values of three types of tasks： the soft, the hard and the imprecise tasks. The simulation...In this paper, a novel scheduling mechanism is proposed to handle the real-time overload problem by maximizing the cumulative values of three types of tasks： the soft, the hard and the imprecise tasks. The simulation results show that the performance of our presented mechanism in this paper is greatly improved, much better than that of the other three mechanisms： earliest deadline first （EDF）, highest value first （HVF） and highest density first （HDF）, under the same conditions of all nominal loads and task type proportions.展开更多
【目的】建立一种快速、灵敏、特异的检测甘蔗条点病毒(sugarcane striate virus,SStrV)的SYBR Green I荧光定量PCR方法。【方法】从SStrV基因组保守区域序列设计特异性扩增引物,构建含有SStrV基因组序列的重组质粒pMD19-T-SStrV-qN作...【目的】建立一种快速、灵敏、特异的检测甘蔗条点病毒(sugarcane striate virus,SStrV)的SYBR Green I荧光定量PCR方法。【方法】从SStrV基因组保守区域序列设计特异性扩增引物,构建含有SStrV基因组序列的重组质粒pMD19-T-SStrV-qN作为阳性质粒标准品,以其为模板建立SStrV荧光定量PCR检测方法,并对该方法的灵敏性、特异性、稳定性进行了测试,随后用该方法对甘蔗不同组织部位中SStrV载量进行检测。【结果】将含有SStrV基因组序列的重组质粒按10倍比稀释成标准品,将其作为模板进行荧光定量PCR,获得标准曲线y=-3.337 x+38.197,相关系数r2=0.999,说明Cq值与标准品浓度拷贝数的对数呈线性关系;建立的荧光定量PCR最低可以检测到13拷贝重组质粒/μL,是普通PCR灵敏度的100倍。该方法能特异的检测SStrV,特异性高,组内和组间的变异系数在0.13%-0.94%之间,表明该方法重复性良好。SStrV的载量在甘蔗的不同组织部位中差异较大,+4叶中SStrV的载量最高,与其他组织部位达到了显著差异。【结论】建立了能灵敏特异检测SStrV的SYBR Green I荧光定量PCR方法,明确了+4叶是甘蔗中SStrV检测的最佳采样部位。展开更多
This paper describes the significant cost saving opportunities for consumers in developing countries by the use of computational intelligence and demand-side-management techniques to mitigate the massive use of diesel...This paper describes the significant cost saving opportunities for consumers in developing countries by the use of computational intelligence and demand-side-management techniques to mitigate the massive use of diesel back-up during grid outages. Application of load scheduling optimization is investigated during scheduled power outages, for residential consumer in India. The specific load shifting approaches explored include a day ahead predicted load schedule which is generated by performing a DSM referring to the forecasted day ahead outage. Whereas in reality the predicted may not match the actual outage, thus in these cases a fuzzy logic rule base is referred on real time basis to take corrective action & reach the best optimal load schedule possible to attain the lowest cost. The load types modeled include passive loads and schedulable, i.e. typically heavy loads. It is found that this multi-level DSM schemes show excellent benefits to the consumer. The maximum diesel savings for the consumer due to load shifting can be approximately ranging from 45% to as high as 75% for a flat-tariff grid. The study also showed that the actual savings potential depends on the timing of power outage, duration and the specific load characteristics.展开更多
AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers...AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.展开更多
基金supported by the special program to enhance the navigation capacity of the Golden Waterway funded by the Ministry of Transport of the People’s Republic of China"Research on Key Techniques to Monitor and Simulate the River Flow and Sediment Transport"(Grant No.2011-328-746-40)
文摘Quantity of bed load is an important physical parameter in sediment transport research. Aiming at the difficulties in the bed load measurement, this paper develops a bottom-mounted monitor to measure the bed load transport rate by adopting the sedimentation pit method and resolving such key problems as weighing and desilting, which can achieve long-time, all-weather and real-time telemeasurement of the bed load transport rate of plain rivers, estuaries and coasts. Both laboratory and field tests show that this monitor is reasonable in design, stable in properties and convenient in measurement, and it can be used to monitor the bed load transport rate in practical projects.
基金supported by the Shanghai Applied Materials Foundation (Grant No.06SA18)
文摘In this paper, a novel scheduling mechanism is proposed to handle the real-time overload problem by maximizing the cumulative values of three types of tasks： the soft, the hard and the imprecise tasks. The simulation results show that the performance of our presented mechanism in this paper is greatly improved, much better than that of the other three mechanisms： earliest deadline first （EDF）, highest value first （HVF） and highest density first （HDF）, under the same conditions of all nominal loads and task type proportions.
文摘【目的】建立一种快速、灵敏、特异的检测甘蔗条点病毒(sugarcane striate virus,SStrV)的SYBR Green I荧光定量PCR方法。【方法】从SStrV基因组保守区域序列设计特异性扩增引物,构建含有SStrV基因组序列的重组质粒pMD19-T-SStrV-qN作为阳性质粒标准品,以其为模板建立SStrV荧光定量PCR检测方法,并对该方法的灵敏性、特异性、稳定性进行了测试,随后用该方法对甘蔗不同组织部位中SStrV载量进行检测。【结果】将含有SStrV基因组序列的重组质粒按10倍比稀释成标准品,将其作为模板进行荧光定量PCR,获得标准曲线y=-3.337 x+38.197,相关系数r2=0.999,说明Cq值与标准品浓度拷贝数的对数呈线性关系;建立的荧光定量PCR最低可以检测到13拷贝重组质粒/μL,是普通PCR灵敏度的100倍。该方法能特异的检测SStrV,特异性高,组内和组间的变异系数在0.13%-0.94%之间,表明该方法重复性良好。SStrV的载量在甘蔗的不同组织部位中差异较大,+4叶中SStrV的载量最高,与其他组织部位达到了显著差异。【结论】建立了能灵敏特异检测SStrV的SYBR Green I荧光定量PCR方法,明确了+4叶是甘蔗中SStrV检测的最佳采样部位。
文摘This paper describes the significant cost saving opportunities for consumers in developing countries by the use of computational intelligence and demand-side-management techniques to mitigate the massive use of diesel back-up during grid outages. Application of load scheduling optimization is investigated during scheduled power outages, for residential consumer in India. The specific load shifting approaches explored include a day ahead predicted load schedule which is generated by performing a DSM referring to the forecasted day ahead outage. Whereas in reality the predicted may not match the actual outage, thus in these cases a fuzzy logic rule base is referred on real time basis to take corrective action & reach the best optimal load schedule possible to attain the lowest cost. The load types modeled include passive loads and schedulable, i.e. typically heavy loads. It is found that this multi-level DSM schemes show excellent benefits to the consumer. The maximum diesel savings for the consumer due to load shifting can be approximately ranging from 45% to as high as 75% for a flat-tariff grid. The study also showed that the actual savings potential depends on the timing of power outage, duration and the specific load characteristics.
基金Supported by Research Fund for the Control of Infectious Diseases and Research Grant Committee of Hong Kong Government
文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.
