To evaluate and improve the real-time performance of Ethernet for plant automation（EPA） industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By...To evaluate and improve the real-time performance of Ethernet for plant automation（EPA） industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By analyzing information transmission regularity and EPA deterministic scheduling mechanism,periodic messages were categorized as different modes according to their entering-queue time.The scheduling characteristics and delivery time of each mode and their interacting relations were studied,during which the models of real-time performance of periodic information transmission in EPA system were established.On this basis,an experimental platform is developed to test the delivery time of periodic messages transmission in EPA system.According to the analysis and the experiment,the main factors that limit the real-time performance of EPA periodic data transmission and the improvement methods were proposed.展开更多
The authors propose a new persistent transmission based real time Ethernet MAC protocol that provides a predictable upper bound for the delivery delay of real time frames. Moreover, it is compatible with the protocol ...The authors propose a new persistent transmission based real time Ethernet MAC protocol that provides a predictable upper bound for the delivery delay of real time frames. Moreover, it is compatible with the protocol used by the existing Ethernet controllers for conventional datagram traffic and thus standard Ethernet stations can be used in the system without any modification. The paper describes the protocol in detail and analyses the maximum delivery delay for real time traffic and the efficiency of the channel.展开更多
Recently,switched Ethernet has become an active area of research because of its wide uses in industry.However,its uses have various real-time constraints on data communications.This paper analyzes the performance of t...Recently,switched Ethernet has become an active area of research because of its wide uses in industry.However,its uses have various real-time constraints on data communications.This paper analyzes the performance of the line topology switched Ethernet as a data acquisition network.Network calculus theory,which has been successfully applied to assess the real-time performance of packet-switched networks,is used to analyze the networks.To properly describe the activity of switches,a novel approach of modeling data flows into or out of switches is addressed.Based on our model,a concisely analytical expression of the maximal end-to-end delay in line topology switched Ethernet is derived.Finally,the relative simulation results are demonstrated.These results agree well with the analytical results,and thus they validate the data flow modeling techniques.展开更多
[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
The present study explores an IEEE1588 Synchronizing System for smart distribution grid based on Industrial Ethernet. The paper first analyzes the communication system in distribution network and then proposed the pro...The present study explores an IEEE1588 Synchronizing System for smart distribution grid based on Industrial Ethernet. The paper first analyzes the communication system in distribution network and then proposed the project of time synchronizing system using IEEE1588 in distribution network. The study focuses on rational clock correcting time region segmentation, selecting the best clock source injection point and multiple redundant methods when correcting time method lose efficacy, etc. The precision of time synchronizing is better than that of 1 millisecond.展开更多
基金Supported by the National High Technology Research and Development Program of China (2006AA040301-4,2007AA041301-6)
文摘To evaluate and improve the real-time performance of Ethernet for plant automation（EPA） industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By analyzing information transmission regularity and EPA deterministic scheduling mechanism,periodic messages were categorized as different modes according to their entering-queue time.The scheduling characteristics and delivery time of each mode and their interacting relations were studied,during which the models of real-time performance of periodic information transmission in EPA system were established.On this basis,an experimental platform is developed to test the delivery time of periodic messages transmission in EPA system.According to the analysis and the experiment,the main factors that limit the real-time performance of EPA periodic data transmission and the improvement methods were proposed.
基金TheNationalNaturalScienceFoundationofChina (No .6 9984 0 0 3)
文摘The authors propose a new persistent transmission based real time Ethernet MAC protocol that provides a predictable upper bound for the delivery delay of real time frames. Moreover, it is compatible with the protocol used by the existing Ethernet controllers for conventional datagram traffic and thus standard Ethernet stations can be used in the system without any modification. The paper describes the protocol in detail and analyses the maximum delivery delay for real time traffic and the efficiency of the channel.
文摘Recently,switched Ethernet has become an active area of research because of its wide uses in industry.However,its uses have various real-time constraints on data communications.This paper analyzes the performance of the line topology switched Ethernet as a data acquisition network.Network calculus theory,which has been successfully applied to assess the real-time performance of packet-switched networks,is used to analyze the networks.To properly describe the activity of switches,a novel approach of modeling data flows into or out of switches is addressed.Based on our model,a concisely analytical expression of the maximal end-to-end delay in line topology switched Ethernet is derived.Finally,the relative simulation results are demonstrated.These results agree well with the analytical results,and thus they validate the data flow modeling techniques.
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
文摘The present study explores an IEEE1588 Synchronizing System for smart distribution grid based on Industrial Ethernet. The paper first analyzes the communication system in distribution network and then proposed the project of time synchronizing system using IEEE1588 in distribution network. The study focuses on rational clock correcting time region segmentation, selecting the best clock source injection point and multiple redundant methods when correcting time method lose efficacy, etc. The precision of time synchronizing is better than that of 1 millisecond.
