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Real-Time Detection and Instance Segmentation of Strawberry in Unstructured Environment
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作者 Chengjun Wang Fan Ding +4 位作者 Yiwen Wang Renyuan Wu Xingyu Yao Chengjie Jiang Liuyi Ling 《Computers, Materials & Continua》 SCIE EI 2024年第1期1481-1501,共21页
The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-r... The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-ronment is a challenging task.Current instance segmentation algorithms for strawberries suffer from issues such as poor real-time performance and low accuracy.To this end,the present study proposes an Efficient YOLACT(E-YOLACT)algorithm for strawberry detection and segmentation based on the YOLACT framework.The key enhancements of the E-YOLACT encompass the development of a lightweight attention mechanism,pyramid squeeze shuffle attention(PSSA),for efficient feature extraction.Additionally,an attention-guided context-feature pyramid network(AC-FPN)is employed instead of FPN to optimize the architecture’s performance.Furthermore,a feature-enhanced model(FEM)is introduced to enhance the prediction head’s capabilities,while efficient fast non-maximum suppression(EF-NMS)is devised to improve non-maximum suppression.The experimental results demonstrate that the E-YOLACT achieves a Box-mAP and Mask-mAP of 77.9 and 76.6,respectively,on the custom dataset.Moreover,it exhibits an impressive category accuracy of 93.5%.Notably,the E-YOLACT also demonstrates a remarkable real-time detection capability with a speed of 34.8 FPS.The method proposed in this article presents an efficient approach for the vision system of a strawberry-picking robot. 展开更多
关键词 YOLACT real-time detection instance segmentation attention mechanism STRAWBERRY
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Real-Time Object Detection and Face Recognition Application for the Visually Impaired
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作者 Karshiev Sanjar Soyoun Bang +1 位作者 SookheeRyue Heechul Jung 《Computers, Materials & Continua》 SCIE EI 2024年第6期3569-3583,共15页
The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional appro... The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional approaches primarily focus on broad applications such as wayfinding,obstacle detection,and fall prevention.However,there is a notable discrepancy in applying these technologies to more specific scenarios,like identifying distinct food crop types or recognizing faces.This study proposes a real-time application designed for visually impaired individuals,aiming to bridge this research-application gap.It introduces a system capable of detecting 20 different food crop types and recognizing faces with impressive accuracies of 83.27%and 95.64%,respectively.These results represent a significant contribution to the field of assistive technologies,providing visually impaired users with detailed and relevant information about their surroundings,thereby enhancing their mobility and ensuring their safety.Additionally,it addresses the vital aspects of social engagements,acknowledging the challenges faced by visually impaired individuals in recognizing acquaintances without auditory or tactile signals,and highlights recent developments in prototype systems aimed at assisting with face recognition tasks.This comprehensive approach not only promises enhanced navigational aids but also aims to enrich the social well-being and safety of visually impaired communities. 展开更多
关键词 Artificial intelligence deep learning real-time object detection application
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Analyzing the Impact of Scene Transitions on Indoor Camera Localization through Scene Change Detection in Real-Time
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作者 Muhammad S.Alam Farhan B.Mohamed +2 位作者 Ali Selamat Faruk Ahmed AKM B.Hossain 《Intelligent Automation & Soft Computing》 2024年第3期417-436,共20页
Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance o... Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance of robotic applications in terms of accuracy and speed.This research proposed a real-time indoor camera localization system based on a recurrent neural network that detects scene change during the image sequence.An annotated image dataset trains the proposed system and predicts the camera pose in real-time.The system mainly improved the localization performance of indoor cameras by more accurately predicting the camera pose.It also recognizes the scene changes during the sequence and evaluates the effects of these changes.This system achieved high accuracy and real-time performance.The scene change detection process was performed using visual rhythm and the proposed recurrent deep architecture,which performed camera pose prediction and scene change impact evaluation.Overall,this study proposed a novel real-time localization system for indoor cameras that detects scene changes and shows how they affect localization performance. 展开更多
关键词 Camera pose estimation indoor camera localization real-time localization scene change detection simultaneous localization and mapping(SLAM)
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A YOLOv8-CE-based real-time traffic sign detection and identification method for autonomous vehicles
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作者 Yuechen Luo Yusheng Ci +1 位作者 Hexin Zhang Lina Wu 《Digital Transportation and Safety》 2024年第3期82-91,共10页
Traffic sign detection in real scenarios is challenging due to their complexity and small size,often preventing existing deep learning models from achieving both high accuracy and real-time performance.An improved YOL... Traffic sign detection in real scenarios is challenging due to their complexity and small size,often preventing existing deep learning models from achieving both high accuracy and real-time performance.An improved YOLOv8 model for traffic sign detection is proposed.Firstly,by adding Coordinate Attention(CA)to the Backbone,the model gains location information,improving detection accuracy.Secondly,we also introduce EIoU to the localization function to address the ambiguity in aspect ratio descriptions by calculating the width-height difference based on CIoU.Additionally,Focal Loss is incorporated to balance sample difficulty,enhancing regression accuracy.Finally,the model,YOLOv8-CE(YOLOv8-Coordinate Attention-EIoU),is tested on the Jetson Nano,achieving real-time street scene detection and outperforming the Raspberry Pi 4B.Experimental results show that YOLOv8-CE excels in various complex scenarios,improving mAP by 2.8%over the original YOLOv8.The model size and computational effort remain similar,with the Jetson Nano achieving an inference time of 96 ms,significantly faster than the Raspberry Pi 4B. 展开更多
关键词 YOLOv8-CE-based real-time Traffic SIGNS detection
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A CNN-Based Single-Stage Occlusion Real-Time Target Detection Method
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作者 Liang Liu Nan Yang +4 位作者 Saifei Liu Yuanyuan Cao Shuowen Tian Tiancheng Liu Xun Zhao 《Journal of Intelligent Learning Systems and Applications》 2024年第1期1-11,共11页
Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The m... Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The method adopts the overall design of backbone network, detection network and algorithmic parameter optimisation method, completes the model training on the self-constructed occlusion target dataset, and adopts the multi-scale perception method for target detection. The HNM algorithm is used to screen positive and negative samples during the training process, and the NMS algorithm is used to post-process the prediction results during the detection process to improve the detection efficiency. After experimental validation, the obtained model has the multi-class average predicted value (mAP) of the dataset. It has general advantages over traditional target detection methods. The detection time of a single target on FDDB dataset is 39 ms, which can meet the need of real-time target detection. In addition, the project team has successfully deployed the method into substations and put it into use in many places in Beijing, which is important for achieving the anomaly of occlusion target detection. 展开更多
关键词 real-time Mask Target CNN (Convolutional Neural Network) Single-Stage detection Multi-Scale Feature Perception
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Development of TaqMan-based Real-time PCR Assay for Detecting Transmissible Gastroenteritis Virus and Its Application in Vaccine Evaluation 被引量:2
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作者 俞正玉 徐向伟 +8 位作者 孙冰 何孔旺 郭容利 杜露平 温立斌 张雪寒 茅爱华 倪艳秀 李彬 《Agricultural Science & Technology》 CAS 2014年第9期1487-1490,共4页
[Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus (TGEV). [Method] Primers and a probe were designed according to the conserved sequence o... [Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus (TGEV). [Method] Primers and a probe were designed according to the conserved sequence of N gene in TGEV genome. After gradient dilution, the recombinant plasmid harboring the N gene was used as a standard for real-time PCR assay to establish the standard curve. [Re- sult] The results showed that the established real-time PCR assay exhibited a good linear relationship within the range of 102-10^10 copies/ul; the correlation coefficient was above 0.99 and the amplification efficiency ranged from 90% to 110%. The de- tection limit of real-time PCR assay for TGEV was 10 copies/μl, suggesting a high sensitivity; there was no cross reaction with other porcine viruses, indicating a good specificity; coefficients of variation within and among batches were lower than 3%, suggesting a good repeatability. The established real-time PCR method could be ap- plied in quantitative analysis and evaluation of the immune efficacy of TGEV vac- cines and detection of TGEV in clinical samples. [Conclusion] The TaqMan-based real-time PCR assay established in this study is highly sensitive and specific, which can provide technical means for the epidemiological survey of TGEV, development of TGEV vaccines and investigation of the pathogenesis of TGE. 展开更多
关键词 Transmissible gastroenteritis virus (TGEV) TaqMan-based real-time PCR: detection
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Real-Time Tunable Gas Sensing Platform Based on SnO_(2) Nanoparticles Activated by Blue Micro-Light-Emitting Diodes
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作者 Gi Baek Nam Jung-El Ryu +25 位作者 Tae Hoon Eom Seung Ju Kim Jun Min Suh Seungmin Lee Sungkyun Choi Cheon Woo Moon Seon Ju Park Soo Min Lee Byungsoo Kim Sung Hyuk Park Jin Wook Yang Sangjin Min Sohyeon Park Sung Hwan Cho Hyuk Jin Kim Sang Eon Jun Tae Hyung Lee Yeong Jae Kim Jae Young Kim Young Joon Hong Jong-In Shim Hyung-Gi Byun Yongjo Park Inkyu Park Sang-Wan Ryu Ho Won Jang 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第12期103-119,共17页
Micro-light-emitting diodes(μLEDs)have gained significant interest as an activation source for gas sensors owing to their advantages,including room temperature operation and low power consumption.However,despite thes... Micro-light-emitting diodes(μLEDs)have gained significant interest as an activation source for gas sensors owing to their advantages,including room temperature operation and low power consumption.However,despite these benefits,challenges still exist such as a limited range of detectable gases and slow response.In this study,we present a blueμLED-integrated light-activated gas sensor array based on SnO_(2)nanoparticles(NPs)that exhibit excellent sensitivity,tunable selectivity,and rapid detection with micro-watt level power consumption.The optimal power forμLED is observed at the highest gas response,supported by finite-difference time-domain simulation.Additionally,we first report the visible light-activated selective detection of reducing gases using noble metal-decorated SnO_(2)NPs.The noble metals induce catalytic interaction with reducing gases,clearly distinguishing NH3,H2,and C2H5OH.Real-time gas monitoring based on a fully hardwareimplemented light-activated sensing array was demonstrated,opening up new avenues for advancements in light-activated electronic nose technologies. 展开更多
关键词 Micro-LED Gas sensor array Low power consumption Metal decoration real-time detection
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LDA-ID:An LDA-Based Framework for Real-Time Network Intrusion Detection 被引量:1
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作者 Weidong Zhou Shengwei Lei +1 位作者 Chunhe Xia Tianbo Wang 《China Communications》 SCIE CSCD 2023年第12期166-181,共16页
Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time ... Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time detection is an urgent problem.To address the two above problems,we propose a Latent Dirichlet Allocation topic model-based framework for real-time network Intrusion Detection(LDA-ID),consisting of static and online LDA-ID.The problem of feature overlap is transformed into static LDA-ID topic number optimization and topic selection.Thus,the detection is based on the latent topic features.To achieve efficient real-time detection,we design an online computing mode for static LDA-ID,in which a parameter iteration method based on momentum is proposed to balance the contribution of prior knowledge and new information.Furthermore,we design two matching mechanisms to accommodate the static and online LDA-ID,respectively.Experimental results on the public NSL-KDD and UNSW-NB15 datasets show that our framework gets higher accuracy than the others. 展开更多
关键词 feature overlap LDA-ID optimal topic number determination real-time intrusion detection
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Force Sensitive Resistors-Based Real-Time Posture Detection System Using Machine Learning Algorithms
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作者 Arsal Javaid Areeb Abbas +4 位作者 Jehangir Arshad Mohammad Khalid Imam Rahmani Sohaib Tahir Chauhdary Mujtaba Hussain Jaffery Abdulbasid S.Banga 《Computers, Materials & Continua》 SCIE EI 2023年第11期1795-1814,共20页
To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Susta... To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Sustainable Development Goal(SDG),ensuring healthy lives and promoting well-being for all ages,as specified by the World Health Organization(WHO).An improper sitting position can be fatal if one sits for a long time in the wrong position,and it can be dangerous for ulcers and lower spine discomfort.This novel study includes a practical implementation of a cushion consisting of a grid of 3×3 force-sensitive resistors(FSR)embedded to read the pressure of the person sitting on it.Additionally,the Body Mass Index(BMI)has been included to increase the resilience of the system across individual physical variances and to identify the incorrect postures(backward,front,left,and right-leaning)based on the five machine learning algorithms:ensemble boosted trees,ensemble bagged trees,ensemble subspace K-Nearest Neighbors(KNN),ensemble subspace discriminant,and ensemble RUSBoosted trees.The proposed arrangement is novel as existing works have only provided simulations without practical implementation,whereas we have implemented the proposed design in Simulink.The results validate the proposed sensor placements,and the machine learning(ML)model reaches a maximum accuracy of 99.99%,which considerably outperforms the existing works.The proposed concept is valuable as it makes it easier for people in workplaces or even at individual household levels to work for long periods without suffering from severe harmful effects from poor posture. 展开更多
关键词 Posture detection FSR sensor machine learning real-time KNN
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Towards Cache-Assisted Hierarchical Detection for Real-Time Health Data Monitoring in IoHT
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作者 Muhammad Tahir Mingchu Li +4 位作者 Irfan Khan Salman AAl Qahtani Rubia Fatima Javed Ali Khan Muhammad Shahid Anwar 《Computers, Materials & Continua》 SCIE EI 2023年第11期2529-2544,共16页
Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the eff... Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the efficacy of big data awareness detection systems.We advocate for a collaborative caching approach involving edge devices and cloud networks to combat this.This strategy is devised to streamline the data retrieval path,subsequently diminishing network strain.Crafting an adept cache processing scheme poses its own set of challenges,especially given the transient nature of monitoring data and the imperative for swift data transmission,intertwined with resource allocation tactics.This paper unveils a novel mobile healthcare solution that harnesses the power of our collaborative caching approach,facilitating nuanced health monitoring via edge devices.The system capitalizes on cloud computing for intricate health data analytics,especially in pinpointing health anomalies.Given the dynamic locational shifts and possible connection disruptions,we have architected a hierarchical detection system,particularly during crises.This system caches data efficiently and incorporates a detection utility to assess data freshness and potential lag in response times.Furthermore,we introduce the Cache-Assisted Real-Time Detection(CARD)model,crafted to optimize utility.Addressing the inherent complexity of the NP-hard CARD model,we have championed a greedy algorithm as a solution.Simulations reveal that our collaborative caching technique markedly elevates the Cache Hit Ratio(CHR)and data freshness,outshining its contemporaneous benchmark algorithms.The empirical results underscore the strength and efficiency of our innovative IoHT-based health monitoring solution.To encapsulate,this paper tackles the nuances of real-time health data monitoring in the IoHT landscape,presenting a joint edge-cloud caching strategy paired with a hierarchical detection system.Our methodology yields enhanced cache efficiency and data freshness.The corroborative numerical data accentuates the feasibility and relevance of our model,casting a beacon for the future trajectory of real-time health data monitoring systems. 展开更多
关键词 real-time health data monitoring Cache-Assisted real-time detection(CARD) edge-cloud collaborative caching scheme hierarchical detection Internet of Health Things(IoHT)
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Cyber Resilience through Real-Time Threat Analysis in Information Security
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作者 Aparna Gadhi Ragha Madhavi Gondu +1 位作者 Hitendra Chaudhary Olatunde Abiona 《International Journal of Communications, Network and System Sciences》 2024年第4期51-67,共17页
This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends t... This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends that they are not the same. The concept of cyber security is explored, which goes beyond protecting information resources to include a wider variety of assets, including people [1]. Protecting information assets is the main goal of traditional information security, with consideration to the human element and how people fit into the security process. On the other hand, cyber security adds a new level of complexity, as people might unintentionally contribute to or become targets of cyberattacks. This aspect presents moral questions since it is becoming more widely accepted that society has a duty to protect weaker members of society, including children [1]. The study emphasizes how important cyber security is on a larger scale, with many countries creating plans and laws to counteract cyberattacks. Nevertheless, a lot of these sources frequently neglect to define the differences or the relationship between information security and cyber security [1]. The paper focus on differentiating between cybersecurity and information security on a larger scale. The study also highlights other areas of cybersecurity which includes defending people, social norms, and vital infrastructure from threats that arise from online in addition to information and technology protection. It contends that ethical issues and the human factor are becoming more and more important in protecting assets in the digital age, and that cyber security is a paradigm shift in this regard [1]. 展开更多
关键词 Cybersecurity Information Security Network Security Cyber Resilience real-time Threat Analysis Cyber Threats Cyberattacks Threat Intelligence Machine Learning Artificial Intelligence Threat detection Threat Mitigation Risk Assessment Vulnerability Management Incident Response Security Orchestration Automation Threat Landscape Cyber-Physical Systems Critical Infrastructure Data Protection Privacy Compliance Regulations Policy Ethics CYBERCRIME Threat Actors Threat Modeling Security Architecture
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Portable FBAR based E-nose for cold chain real-time bananas shelf time detection
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作者 Chen Wu Jiuyan Li 《Nanotechnology and Precision Engineering》 CAS CSCD 2023年第1期32-39,共8页
Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and... Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and cumulative testing.Also,an ideal electronic nose(E-nose)in a cold chain should be stable to its surroundings and remain highly accurate and portable.In this work,a portable film bulk acoustic resonator(FBAR)-based E-nose was built for real-time measurement of banana shelf time.The sensor chamber to contain the portable circuit of the E-nose is as small as a smartphone,and by introducing an air-tight FBAR as a reference,the E-nose can avoid most of the drift caused by surroundings.With the help of porous layer by layer(LBL)coating of the FBAR,the sensitivity of the E-nose is 5 ppm to ethylene and 0.5 ppm to isoamyl acetate and isoamyl butyrate,while the detection range is large enough to cover a relative humidity of 0.8.In this regard,the E-nose can easily discriminate between yellow bananas with green necks and entirely yellow bananas while allowing the bananas to maintain their biological activities in their normal storage state,thereby showing the possibility of real-time shelf time detection.This portable FBAR-based E-nose has a large testing scale,high sensitivity,good humidity tolerance,and low frequency drift to its surroundings,thereby meeting the needs of cold-chain usage. 展开更多
关键词 Film bulk acoustic resonator(FBAR) Portable E-nose real-time detection Layer by layer
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Real-Time Method for Detecting Harmonic and Reactive Currents of Single-Phase Circuits 被引量:1
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作者 张秀峰 丁菊霞 《Journal of Southwest Jiaotong University(English Edition)》 2006年第2期135-141,共7页
According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. Th... According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. The results of theoretical analysis and simula- tion show that the proposed method is effective in realtime detecting of instantaneous harmonic and reactive currents in single-phase circuits. When only detecting the total reactive currents, this method does not need a phase-locked loop circuit, and it also can be used in some special applications to provide different compensations on the ground of different requirements of electric network. Compared with the other methods based on the theory of instantaneous reactive power, this method is simple and easy to realize. 展开更多
关键词 Active filter HARMONIC Reactive current real-time detection Single-phase circuit Electric-network
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A Method for Detecting Intrusion on Networks in Real-time Based on IP Weight
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作者 黄本雄 Lu +2 位作者 Wei Huang Zailu 《High Technology Letters》 EI CAS 2001年第2期34-38,共5页
A new rule to detect intrusion based on IP weight, which is also well implemented in the rule base of author’s NMS, is presented. Compared with traditional ones, intrusion detecting based on IP weight enhanced analys... A new rule to detect intrusion based on IP weight, which is also well implemented in the rule base of author’s NMS, is presented. Compared with traditional ones, intrusion detecting based on IP weight enhanced analysis to packet content. The method also provides a real-time efficient way to analyze traffic on high-speed network and can help to increase valid usage rates of network resources. Practical implementation as a rule in the rule base of our NMS has verified that the rule can detect not only attacks on network, but also other unusual behaviors. 展开更多
关键词 Network security Intrusion detecting IP weight detection of attacks real-time analysis
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Synchronously Detecting Allergenic Ingredients of Peanut and Sesame in Food by Real-time Fluorescent PCR
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作者 Yongxin WANG Xiao CHENG +3 位作者 Yeju LU Hong AN Bo ZHANG Juanjuan LIU 《Agricultural Biotechnology》 CAS 2014年第3期1-3,共3页
Peanut,sesame and other raw materials of food are allergens for special populations.In this study,specific primers and TaqMan probes labeled by different fluorescences were designed targeting Ara h 2 gene of peanut an... Peanut,sesame and other raw materials of food are allergens for special populations.In this study,specific primers and TaqMan probes labeled by different fluorescences were designed targeting Ara h 2 gene of peanut and Ses i 1 gene of sesame.After the optimization of reaction conditions,a real-time fluorescent PCR method was established for simultaneous detection of allergenic ingredients of peanut and sesame in food.Genomic DNA samples of peanut,sesame,rice,wheat,barley,soybean,celery,maize,potato,tomato,walnut,groundnut in shell,cashew nut,sunflower seed,almond,apple,pear and strawberry,pork,beef,mutton and fish were used as templates for PCR amplification with deionized water as negative control template.Results indicated that the established real-time fluorescent PCR method could specifically identify allergenic ingredients of peanut and sesame simultaneously.Sensitivity test showed that the minimum detection limit of this method was 0.01%.Therefore,the established real-time fluorescent PCR method is a specific,sensitive and effective assay for simultaneously detecting allergenic ingredients of peanut and sesame in food. 展开更多
关键词 real-time fluorescent PCR PEANUT SESAME Allergen detection
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Development and Preliminary Application of SYBR Green I Real-Time Fluorescence Quantitative PCR Method for Detecting Porcine Parvovirus Virus
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作者 SHEN Zhi-qiang WANG Jin-liang +3 位作者 GUO Xian-po WANG Xiao-hu WANG Ming ZHAO De-ming 《Animal Husbandry and Feed Science》 CAS 2009年第11期42-46,共5页
According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2 (NC001718) available in GenBank (NC_001718), a pair of specific primer was designed, and the target fragment of 431 bp was obtained by P... According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2 (NC001718) available in GenBank (NC_001718), a pair of specific primer was designed, and the target fragment of 431 bp was obtained by PCR amplification. The products were ligated with pMD18- T vector and then transformed into bacteria DH5α for recombinant plasmid extraction. After PCR identification and sequencing, recombinant plasmid was used as a standard template to establish the standard curve of SYBR Green I fluorescence quantitative PCR. Sensitivity test, specificity test and repeatability test were also determined. The results indicated that there was a good linear relationship between threshold cycle of the standard curve and template concentration, R2 =0.997 6. Tm ranged from 82.3 to 82.9 ℃, while the sensitivity was 72.1 copies/μl with good specificity and repeatability. The developed SYBR Green I real-time quantitative PCR method to detect PPV VP2 gene laid the basis for further studies on patho- oenesis, early clinical diaonosis of this virus and quantitative analysis of PPV infection. 展开更多
关键词 Porcine parvovirus virus real-time fluorescence quantitative PCR detectION
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Complete genome sequence of a Rodent Torque teno virus in Hainan Island, China and establishment of a real-time PCR for detecting Rodent Torque teno virus 3
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作者 Yue Wu Shan-Shan Wang +12 位作者 Wen-Qi Wang Huan-Huan Zhou Jin-Long Chen Yu-Fang Yi Tian-Ming Ma Xiu-Ji Cui Yi Huang Gao-Yu Wang Ruo-Yan Peng Xiao-Yuan Hu Chang-Hua He Gang Lu Fei-Fei Yin 《Journal of Hainan Medical University》 2019年第4期1-6,共6页
Objective:To perform whole-genome sequencing and phylogenetic analysis of the local endemic strain of Rodent Torque teno virus (RoTTV), RoTTV3-HMU1, found in Rattus norvegicus, Haikou City, Hainan Province, and establ... Objective:To perform whole-genome sequencing and phylogenetic analysis of the local endemic strain of Rodent Torque teno virus (RoTTV), RoTTV3-HMU1, found in Rattus norvegicus, Haikou City, Hainan Province, and establish a SYBR Green I based real-time PCR detection assay for RoTTV3.Methods: Based on the high-throughput genome sequencing analysis, specific primers were designed and the whole genome sequence was amplified by PCR and Sanger sequencing. Specific detection primers were designed based on the conserved sequences of RoTTV3. The recombinant plasmid contained the whole genome of RoTTV3-HMU1 was constructed as a standard control. The experimental conditions were optimized and the real-time PCR detection assay of RoTTV3 was established.Results: The genomic sequence of RoTTV carried by Rattus norvegicus in Haikou City was successfully sequenced. Phylogenetic analysis indicated that the virus belongs to the RoTTV3 genotype. In this experiment, the real-time PCR detection method of RoTTV3 was established. The standard curve generated had a wide dynamic range from 1×(102-108) copies/μL, with a linear correlation (R2=1.000). The melting curve analysis using SYBR Green showed only one specific melting peak and no primer-dimmers represented. The detection limit was 100 copies/reaction.Discussion: This study was the first report of the RoTTV in Hainan Islands, and its phylogenetic analysis was of great significance to the origin and evolution of RoTTV. The RoTTV3 real-time PCR detection method established in this experiment has a high sensitivity and good specificity, which lays a technical foundation for the epidemiological investigation of RoTTV3. 展开更多
关键词 RODENT TORQUE teno virus WHOLE-GENOME SEQUENCING real-time PCR detection ASSAY
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Real-time image processing and display in object size detection based on VC++ 被引量:2
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作者 翟亚宇 潘晋孝 +1 位作者 刘宾 陈平 《Journal of Measurement Science and Instrumentation》 CAS 2014年第4期40-45,共6页
Real-time detection for object size has now become a hot topic in the testing field and image processing is the core algorithm. This paper focuses on the processing and display of the collected dynamic images to achie... Real-time detection for object size has now become a hot topic in the testing field and image processing is the core algorithm. This paper focuses on the processing and display of the collected dynamic images to achieve a real-time image pro- cessing for the moving objects. Firstly, the median filtering, gain calibration, image segmentation, image binarization, cor- ner detection and edge fitting are employed to process the images of the moving objects to make the image close to the real object. Then, the processed images are simultaneously displayed on a real-time basis to make it easier to analyze, understand and identify them, and thus it reduces the computation complexity. Finally, human-computer interaction (HCI)-friendly in- terface based on VC ++ is designed to accomplish the digital logic transform, image processing and real-time display of the objects. The experiment shows that the proposed algorithm and software design have better real-time performance and accu- racy which can meet the industrial needs. 展开更多
关键词 size detection real-time image processing and display gain calibration edge fitting
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FIR-YOLACT:Fusion of ICIoU and Res2Net for YOLACT on Real-Time Vehicle Instance Segmentation 被引量:1
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作者 Wen Dong Ziyan Liu +1 位作者 Mo Yang Ying Wu 《Computers, Materials & Continua》 SCIE EI 2023年第12期3551-3572,共22页
Autonomous driving technology has made a lot of outstanding achievements with deep learning,and the vehicle detection and classification algorithm has become one of the critical technologies of autonomous driving syst... Autonomous driving technology has made a lot of outstanding achievements with deep learning,and the vehicle detection and classification algorithm has become one of the critical technologies of autonomous driving systems.The vehicle instance segmentation can perform instance-level semantic parsing of vehicle information,which is more accurate and reliable than object detection.However,the existing instance segmentation algorithms still have the problems of poor mask prediction accuracy and low detection speed.Therefore,this paper proposes an advanced real-time instance segmentation model named FIR-YOLACT,which fuses the ICIoU(Improved Complete Intersection over Union)and Res2Net for the YOLACT algorithm.Specifically,the ICIoU function can effectively solve the degradation problem of the original CIoU loss function,and improve the training convergence speed and detection accuracy.The Res2Net module fused with the ECA(Efficient Channel Attention)Net is added to the model’s backbone network,which improves the multi-scale detection capability and mask prediction accuracy.Furthermore,the Cluster NMS(Non-Maximum Suppression)algorithm is introduced in the model’s bounding box regression to enhance the performance of detecting similarly occluded objects.The experimental results demonstrate the superiority of FIR-YOLACT to the based methods and the effectiveness of all components.The processing speed reaches 28 FPS,which meets the demands of real-time vehicle instance segmentation. 展开更多
关键词 Instance segmentation real-time vehicle detection YOLACT Res2Net ICIoU
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Real-Time Detection of Cracks on Concrete Bridge Decks Using Deep Learning in the Frequency Domain 被引量:10
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作者 Qianyun Zhang Kaveh Barri +1 位作者 Saeed K.Babanajad Amir H.Alavi 《Engineering》 SCIE EI 2021年第12期1786-1796,共11页
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequen... This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM algorithm is trained using thousands of images of cracked and non-cracked concrete bridge decks.In order to improve the training efficiency,images are first transformed into the frequency domain during a preprocessing phase.The algorithm is then calibrated using the flattened frequency data.LSTM is used to improve the performance of the developed network for long sequence data.The accuracy of the developed model is 99.05%,98.9%,and 99.25%,respectively,for training,validation,and testing data.An implementation framework is further developed for future application of the trained model for large-scale images.The proposed 1D-CNN-LSTM method exhibits superior performance in comparison with existing deep learning methods in terms of accuracy and computation time.The fast implementation of the 1D-CNN-LSTM algorithm makes it a promising tool for real-time crack detection. 展开更多
关键词 Crack detection Concrete bridge deck Deep learning real-time
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