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A Robust Approach for Multi Classification-Based Intrusion Detection through Stacking Deep Learning Models
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作者 Samia Allaoua Chelloug 《Computers, Materials & Continua》 SCIE EI 2024年第6期4845-4861,共17页
Intrusion detection is a predominant task that monitors and protects the network infrastructure.Therefore,many datasets have been published and investigated by researchers to analyze and understand the problem of intr... Intrusion detection is a predominant task that monitors and protects the network infrastructure.Therefore,many datasets have been published and investigated by researchers to analyze and understand the problem of intrusion prediction and detection.In particular,the Network Security Laboratory-Knowledge Discovery in Databases(NSL-KDD)is an extensively used benchmark dataset for evaluating intrusion detection systems(IDSs)as it incorporates various network traffic attacks.It is worth mentioning that a large number of studies have tackled the problem of intrusion detection using machine learning models,but the performance of these models often decreases when evaluated on new attacks.This has led to the utilization of deep learning techniques,which have showcased significant potential for processing large datasets and therefore improving detection accuracy.For that reason,this paper focuses on the role of stacking deep learning models,including convolution neural network(CNN)and deep neural network(DNN)for improving the intrusion detection rate of the NSL-KDD dataset.Each base model is trained on the NSL-KDD dataset to extract significant features.Once the base models have been trained,the stacking process proceeds to the second stage,where a simple meta-model has been trained on the predictions generated from the proposed base models.The combination of the predictions allows the meta-model to distinguish different classes of attacks and increase the detection rate.Our experimental evaluations using the NSL-KDD dataset have shown the efficacy of stacking deep learning models for intrusion detection.The performance of the ensemble of base models,combined with the meta-model,exceeds the performance of individual models.Our stacking model has attained an accuracy of 99%and an average F1-score of 93%for the multi-classification scenario.Besides,the training time of the proposed ensemble model is lower than the training time of benchmark techniques,demonstrating its efficiency and robustness. 展开更多
关键词 Intrusion detection multi classification deep learning STACKING NSL-KDD
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Real-Time Detection and Instance Segmentation of Strawberry in Unstructured Environment
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作者 Chengjun Wang Fan Ding +4 位作者 Yiwen Wang Renyuan Wu Xingyu Yao Chengjie Jiang Liuyi Ling 《Computers, Materials & Continua》 SCIE EI 2024年第1期1481-1501,共21页
The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-r... The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-ronment is a challenging task.Current instance segmentation algorithms for strawberries suffer from issues such as poor real-time performance and low accuracy.To this end,the present study proposes an Efficient YOLACT(E-YOLACT)algorithm for strawberry detection and segmentation based on the YOLACT framework.The key enhancements of the E-YOLACT encompass the development of a lightweight attention mechanism,pyramid squeeze shuffle attention(PSSA),for efficient feature extraction.Additionally,an attention-guided context-feature pyramid network(AC-FPN)is employed instead of FPN to optimize the architecture’s performance.Furthermore,a feature-enhanced model(FEM)is introduced to enhance the prediction head’s capabilities,while efficient fast non-maximum suppression(EF-NMS)is devised to improve non-maximum suppression.The experimental results demonstrate that the E-YOLACT achieves a Box-mAP and Mask-mAP of 77.9 and 76.6,respectively,on the custom dataset.Moreover,it exhibits an impressive category accuracy of 93.5%.Notably,the E-YOLACT also demonstrates a remarkable real-time detection capability with a speed of 34.8 FPS.The method proposed in this article presents an efficient approach for the vision system of a strawberry-picking robot. 展开更多
关键词 YOLACT real-time detection instance segmentation attention mechanism STRAWBERRY
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A Novel Approach to Breast Tumor Detection: Enhanced Speckle Reduction and Hybrid Classification in Ultrasound Imaging
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作者 K.Umapathi S.Shobana +5 位作者 Anand Nayyar Judith Justin R.Vanithamani Miguel Villagómez Galindo Mushtaq Ahmad Ansari Hitesh Panchal 《Computers, Materials & Continua》 SCIE EI 2024年第5期1875-1901,共27页
Breast cancer detection heavily relies on medical imaging, particularly ultrasound, for early diagnosis and effectivetreatment. This research addresses the challenges associated with computer-aided diagnosis (CAD) of ... Breast cancer detection heavily relies on medical imaging, particularly ultrasound, for early diagnosis and effectivetreatment. This research addresses the challenges associated with computer-aided diagnosis (CAD) of breastcancer fromultrasound images. The primary challenge is accurately distinguishing between malignant and benigntumors, complicated by factors such as speckle noise, variable image quality, and the need for precise segmentationand classification. The main objective of the research paper is to develop an advanced methodology for breastultrasound image classification, focusing on speckle noise reduction, precise segmentation, feature extraction, andmachine learning-based classification. A unique approach is introduced that combines Enhanced Speckle ReducedAnisotropic Diffusion (SRAD) filters for speckle noise reduction, U-NET-based segmentation, Genetic Algorithm(GA)-based feature selection, and Random Forest and Bagging Tree classifiers, resulting in a novel and efficientmodel. To test and validate the hybrid model, rigorous experimentations were performed and results state thatthe proposed hybrid model achieved accuracy rate of 99.9%, outperforming other existing techniques, and alsosignificantly reducing computational time. This enhanced accuracy, along with improved sensitivity and specificity,makes the proposed hybrid model a valuable addition to CAD systems in breast cancer diagnosis, ultimatelyenhancing diagnostic accuracy in clinical applications. 展开更多
关键词 Ultrasound images breast cancer tumor classification SEGMENTATION deep learning lesion detection
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Real-Time Object Detection and Face Recognition Application for the Visually Impaired
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作者 Karshiev Sanjar Soyoun Bang +1 位作者 SookheeRyue Heechul Jung 《Computers, Materials & Continua》 SCIE EI 2024年第6期3569-3583,共15页
The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional appro... The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional approaches primarily focus on broad applications such as wayfinding,obstacle detection,and fall prevention.However,there is a notable discrepancy in applying these technologies to more specific scenarios,like identifying distinct food crop types or recognizing faces.This study proposes a real-time application designed for visually impaired individuals,aiming to bridge this research-application gap.It introduces a system capable of detecting 20 different food crop types and recognizing faces with impressive accuracies of 83.27%and 95.64%,respectively.These results represent a significant contribution to the field of assistive technologies,providing visually impaired users with detailed and relevant information about their surroundings,thereby enhancing their mobility and ensuring their safety.Additionally,it addresses the vital aspects of social engagements,acknowledging the challenges faced by visually impaired individuals in recognizing acquaintances without auditory or tactile signals,and highlights recent developments in prototype systems aimed at assisting with face recognition tasks.This comprehensive approach not only promises enhanced navigational aids but also aims to enrich the social well-being and safety of visually impaired communities. 展开更多
关键词 Artificial intelligence deep learning real-time object detection application
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Analyzing the Impact of Scene Transitions on Indoor Camera Localization through Scene Change Detection in Real-Time
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作者 Muhammad S.Alam Farhan B.Mohamed +2 位作者 Ali Selamat Faruk Ahmed AKM B.Hossain 《Intelligent Automation & Soft Computing》 2024年第3期417-436,共20页
Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance o... Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance of robotic applications in terms of accuracy and speed.This research proposed a real-time indoor camera localization system based on a recurrent neural network that detects scene change during the image sequence.An annotated image dataset trains the proposed system and predicts the camera pose in real-time.The system mainly improved the localization performance of indoor cameras by more accurately predicting the camera pose.It also recognizes the scene changes during the sequence and evaluates the effects of these changes.This system achieved high accuracy and real-time performance.The scene change detection process was performed using visual rhythm and the proposed recurrent deep architecture,which performed camera pose prediction and scene change impact evaluation.Overall,this study proposed a novel real-time localization system for indoor cameras that detects scene changes and shows how they affect localization performance. 展开更多
关键词 Camera pose estimation indoor camera localization real-time localization scene change detection simultaneous localization and mapping(SLAM)
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A CNN-Based Single-Stage Occlusion Real-Time Target Detection Method
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作者 Liang Liu Nan Yang +4 位作者 Saifei Liu Yuanyuan Cao Shuowen Tian Tiancheng Liu Xun Zhao 《Journal of Intelligent Learning Systems and Applications》 2024年第1期1-11,共11页
Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The m... Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The method adopts the overall design of backbone network, detection network and algorithmic parameter optimisation method, completes the model training on the self-constructed occlusion target dataset, and adopts the multi-scale perception method for target detection. The HNM algorithm is used to screen positive and negative samples during the training process, and the NMS algorithm is used to post-process the prediction results during the detection process to improve the detection efficiency. After experimental validation, the obtained model has the multi-class average predicted value (mAP) of the dataset. It has general advantages over traditional target detection methods. The detection time of a single target on FDDB dataset is 39 ms, which can meet the need of real-time target detection. In addition, the project team has successfully deployed the method into substations and put it into use in many places in Beijing, which is important for achieving the anomaly of occlusion target detection. 展开更多
关键词 real-time Mask Target CNN (Convolutional Neural Network) Single-Stage detection Multi-Scale Feature Perception
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Real-time image processing and display in object size detection based on VC++ 被引量:2
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作者 翟亚宇 潘晋孝 +1 位作者 刘宾 陈平 《Journal of Measurement Science and Instrumentation》 CAS 2014年第4期40-45,共6页
Real-time detection for object size has now become a hot topic in the testing field and image processing is the core algorithm. This paper focuses on the processing and display of the collected dynamic images to achie... Real-time detection for object size has now become a hot topic in the testing field and image processing is the core algorithm. This paper focuses on the processing and display of the collected dynamic images to achieve a real-time image pro- cessing for the moving objects. Firstly, the median filtering, gain calibration, image segmentation, image binarization, cor- ner detection and edge fitting are employed to process the images of the moving objects to make the image close to the real object. Then, the processed images are simultaneously displayed on a real-time basis to make it easier to analyze, understand and identify them, and thus it reduces the computation complexity. Finally, human-computer interaction (HCI)-friendly in- terface based on VC ++ is designed to accomplish the digital logic transform, image processing and real-time display of the objects. The experiment shows that the proposed algorithm and software design have better real-time performance and accu- racy which can meet the industrial needs. 展开更多
关键词 size detection real-time image processing and display gain calibration edge fitting
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Real-Time Detection of Cracks on Concrete Bridge Decks Using Deep Learning in the Frequency Domain 被引量:10
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作者 Qianyun Zhang Kaveh Barri +1 位作者 Saeed K.Babanajad Amir H.Alavi 《Engineering》 SCIE EI 2021年第12期1786-1796,共11页
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequen... This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM algorithm is trained using thousands of images of cracked and non-cracked concrete bridge decks.In order to improve the training efficiency,images are first transformed into the frequency domain during a preprocessing phase.The algorithm is then calibrated using the flattened frequency data.LSTM is used to improve the performance of the developed network for long sequence data.The accuracy of the developed model is 99.05%,98.9%,and 99.25%,respectively,for training,validation,and testing data.An implementation framework is further developed for future application of the trained model for large-scale images.The proposed 1D-CNN-LSTM method exhibits superior performance in comparison with existing deep learning methods in terms of accuracy and computation time.The fast implementation of the 1D-CNN-LSTM algorithm makes it a promising tool for real-time crack detection. 展开更多
关键词 Crack detection Concrete bridge deck Deep learning real-time
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Intrusion detection using rough set classification 被引量:16
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作者 张连华 张冠华 +2 位作者 郁郎 张洁 白英彩 《Journal of Zhejiang University Science》 EI CSCD 2004年第9期1076-1086,共11页
Recently machine learning-based intrusion detection approaches have been subjected to extensive researches because they can detect both misuse and anomaly. In this paper, rough set classification (RSC), a modern learn... Recently machine learning-based intrusion detection approaches have been subjected to extensive researches because they can detect both misuse and anomaly. In this paper, rough set classification (RSC), a modern learning algorithm, is used to rank the features extracted for detecting intrusions and generate intrusion detection models. Feature ranking is a very critical step when building the model. RSC performs feature ranking before generating rules, and converts the feature ranking to minimal hitting set problem addressed by using genetic algorithm (GA). This is done in classical approaches using Support Vector Machine (SVM) by executing many iterations, each of which removes one useless feature. Compared with those methods, our method can avoid many iterations. In addition, a hybrid genetic algorithm is proposed to increase the convergence speed and decrease the training time of RSC. The models generated by RSC take the form of'IF-THEN' rules, which have the advantage of explication. Tests and comparison of RSC with SVM on DARPA benchmark data showed that for Probe and DoS attacks both RSC and SVM yielded highly accurate results (greater than 99% accuracy on testing set). 展开更多
关键词 Intrusion detection Rough set classification Support vector machine Genetic algorithm
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A fast and adaptive method for automatic weld defect detection in various real-time X-ray imaging systems 被引量:10
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作者 邵家鑫 都东 +2 位作者 石涵 常保华 郭桂林 《China Welding》 EI CAS 2012年第1期8-12,共5页
A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of me... A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of median filter is used to estimate the weld background. After the weld background is subtracted from the original image, an adaptite threshold segmentation algorithm is proposed to obtain the binary image, and then the morphological close and open operation, labeling algorithm and fids'e alarm eliminating algorithm are applied to pracess the binary image to obtain the defect, ct detection result. At last, a fast realization procedure jbr proposed method is developed. The proposed method is tested in real-time X-ray image,s obtairted in different X-ray imaging sutems. Experiment results show that the proposed method is effective to detect low contrast weld dejects with few .false alarms and is adaptive to various types of real-time X-ray imaging systems. 展开更多
关键词 non-destructive testing real-time X-ray imaging weld defect automatie detection
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Algorithmic Scheme for Concurrent Detection and Classification of Printed Circuit Board Defects 被引量:7
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作者 Jakkrit Onshaunjit Jakkree Srinonchat 《Computers, Materials & Continua》 SCIE EI 2022年第4期355-367,共13页
An ideal printed circuit board(PCB)defect inspection system can detect defects and classify PCB defect types.Existing defect inspection technologies can identify defects but fail to classify all PCB defect types.This ... An ideal printed circuit board(PCB)defect inspection system can detect defects and classify PCB defect types.Existing defect inspection technologies can identify defects but fail to classify all PCB defect types.This research thus proposes an algorithmic scheme that can detect and categorize all 14-known PCB defect types.In the proposed algorithmic scheme,fuzzy cmeans clustering is used for image segmentation via image subtraction prior to defect detection.Arithmetic and logic operations,the circle hough transform(CHT),morphological reconstruction(MR),and connected component labeling(CCL)are used in defect classification.The algorithmic scheme achieves 100%defect detection and 99.05%defect classification accuracies.The novelty of this research lies in the concurrent use of CHT,MR,and CCL algorithms to accurately detect and classify all 14-known PCB defect types and determine the defect characteristics such as the location,area,and nature of defects.This information is helpful in electronic parts manufacturing for finding the root causes of PCB defects and appropriately adjusting the manufacturing process.Moreover,the algorithmic scheme can be integrated into machine vision to streamline the manufacturing process,improve the PCB quality,and lower the production cost. 展开更多
关键词 PCB inspection PCB defect types defect detection defect classification image processing
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Detection and classification of breast lesions using multiple information on contrast-enhanced mammography by a multiprocess deep-learning system: A multicenter study 被引量:4
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作者 Yuqian Chen Zhen Hua +16 位作者 Fan Lin Tiantian Zheng Heng Zhou Shijie Zhang Jing Gao Zhongyi Wang Huafei Shao Wenjuan Li Fengjie Liu Simin Wang Yan Zhang Feng Zhao Hao Liu Haizhu Xie Heng Ma Haicheng Zhang Ning Mao 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2023年第4期408-423,共16页
Objective: Accurate detection and classification of breast lesions in early stage is crucial to timely formulate effective treatments for patients. We aim to develop a fully automatic system to detect and classify bre... Objective: Accurate detection and classification of breast lesions in early stage is crucial to timely formulate effective treatments for patients. We aim to develop a fully automatic system to detect and classify breast lesions using multiple contrast-enhanced mammography(CEM) images.Methods: In this study, a total of 1,903 females who underwent CEM examination from three hospitals were enrolled as the training set, internal testing set, pooled external testing set and prospective testing set. Here we developed a CEM-based multiprocess detection and classification system(MDCS) to perform the task of detection and classification of breast lesions. In this system, we introduced an innovative auxiliary feature fusion(AFF)algorithm that could intelligently incorporates multiple types of information from CEM images. The average freeresponse receiver operating characteristic score(AFROC-Score) was presented to validate system’s detection performance, and the performance of classification was evaluated by area under the receiver operating characteristic curve(AUC). Furthermore, we assessed the diagnostic value of MDCS through visual analysis of disputed cases,comparing its performance and efficiency with that of radiologists and exploring whether it could augment radiologists’ performance.Results: On the pooled external and prospective testing sets, MDCS always maintained a high standalone performance, with AFROC-Scores of 0.953 and 0.963 for detection task, and AUCs for classification were 0.909[95% confidence interval(95% CI): 0.822-0.996] and 0.912(95% CI: 0.840-0.985), respectively. It also achieved higher sensitivity than all senior radiologists and higher specificity than all junior radiologists on pooled external and prospective testing sets. Moreover, MDCS performed superior diagnostic efficiency with an average reading time of 5 seconds, compared to the radiologists’ average reading time of 3.2 min. The average performance of all radiologists was also improved to varying degrees with MDCS assistance.Conclusions: MDCS demonstrated excellent performance in the detection and classification of breast lesions,and greatly enhanced the overall performance of radiologists. 展开更多
关键词 Deep learning contrast-enhanced mammography breast lesions detection classification
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Breast Lesions Detection and Classification via YOLO-Based Fusion Models 被引量:4
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作者 Asma Baccouche Begonya Garcia-Zapirain +1 位作者 Cristian Castillo Olea Adel S.Elmaghraby 《Computers, Materials & Continua》 SCIE EI 2021年第10期1407-1425,共19页
With recent breakthroughs in artificial intelligence,the use of deep learning models achieved remarkable advances in computer vision,ecommerce,cybersecurity,and healthcare.Particularly,numerous applications provided e... With recent breakthroughs in artificial intelligence,the use of deep learning models achieved remarkable advances in computer vision,ecommerce,cybersecurity,and healthcare.Particularly,numerous applications provided efficient solutions to assist radiologists for medical imaging analysis.For instance,automatic lesion detection and classification in mammograms is still considered a crucial task that requires more accurate diagnosis and precise analysis of abnormal lesions.In this paper,we propose an end-to-end system,which is based on You-Only-Look-Once(YOLO)model,to simultaneously localize and classify suspicious breast lesions from entire mammograms.The proposed system first preprocesses the raw images,then recognizes abnormal regions as breast lesions and determines their pathology classification as either mass or calcification.We evaluated the model on two publicly available datasets,with 2907 mammograms from the Curated Breast Imaging Subset of Digital Database for Screening Mammography(CBIS-DDSM)and 235 mammograms from INbreast database.We also used a privately collected dataset with 487 mammograms.Furthermore,we suggested a fusion models approach to report more precise detection and accurate classification.Our best results reached a detection accuracy rate of 95.7%,98.1%and 98%for mass lesions and 74.4%,71.8%and 73.2%for calcification lesions,respectively on CBIS-DDSM,INbreast and the private dataset. 展开更多
关键词 Breast cancer detection classification YOLO deep learning FUSION
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Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice 被引量:5
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作者 FANG Yuan XU Li-hui TIAN Wen-xiao HUAI Yan YU Shan-hong LOU Miao-miao XIE Guan-lin 《Rice science》 SCIE 2009年第2期157-160,共4页
Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further ... Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100. 展开更多
关键词 Burkholderia glumae bacterial grain rot detection real-time fluorescence polymerase chain reaction DCE
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Comparison of ligase detection reaction and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B 被引量:3
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作者 Xiao-Ling Wang Song-Gang Xie +3 位作者 Ling Zhang Wei-Xia Yang Xing Wang Hong-Zhi Jin 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第1期120-124,共5页
AIM: To compare the ligase detection reaction (LDR) and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B infection.METHODS: Mixtures of plasmids and serum samples from 52 c... AIM: To compare the ligase detection reaction (LDR) and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B infection.METHODS: Mixtures of plasmids and serum samples from 52 chronic hepatitis B patients with low abundant lamivudine-resistant mutations were tested with LDR and real-time PCR. Time required and reagent cost for both assays were evaluated.RESULTS: Real-time PCR detected 100, 50, 10, 1 and 0.1% of YIDD plasmid, whereas LDR detected 100, 50, 10, 1, 0.1, and 0.01% of YIDD plasmid, in mixtures with YMDD plasmid of 106 copies/mL. Among the 52 clinical serum samples, completely concordant results were obtained for all samples by both assays, and 39 YIDD, 9 YVDD, and 4 YIDD/YVDD were detected. Cost and time required for LDR and real-time PCR are 60/80 CNY (8/10.7 US dollars) and 4.5/2.5 h, respectively.CONCLUSION: LDR and real-time PCR are both sensitive and inexpensive methods for monitoring low abundant YMDD mutants during lamivudine therapy in patients with chronic hepatitis B. LDR is more sensitive and less expensive, while real-time PCR is more rapid. 展开更多
关键词 YMDD mutants Hepatitis B virus real-time PCR Ligase detection reaction
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A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda 被引量:2
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作者 谢国驷 黄倢 +4 位作者 张庆利 韩娜娜 史成银 王秀华 刘庆慧 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第5期731-737,共7页
Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed ur... Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed urgently. To achieve this purpose, we developed a TaqMan-based real-time PCR assay for detection and quantification orE. tarda. The assay targets the hemolysin activator HlyB domain protein of E. tarda. Our optimized TaqMan assay is capable of detecting as little as 40 fg of genomic DNA per reaction. A standard curve was generated from the threshold cycle values (y) against log10 (E. tarda genomic DNA concentration) as x. The intra- and inter-assay coefficient of variation (CV) values were less than 2.06% and 1.05% respectively, indicating that the assay had good reproducibility. This method is highly specific to E. tarda strains, as it shows no cross-reactivity to Edwardsiella ictaluri, a member of the same genus, or to nine other fish-pathogenic bacteria species belonging to three other genera. This sensitive and specific real-time PCR assay provides a valuable tool for diagnostic quantitation of E. tarda in clinical samples. 展开更多
关键词 Edwardsiella tarda TAQMAN real-time PCR detection
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Development and validation of a TaqMan^(TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda 被引量:2
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作者 XIE Guosi HUANG Jie +3 位作者 ZHANG Qingli HAN Nana SHI Chengyin WANG Xiuhua 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第4期140-148,共9页
Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In t... Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQ- PCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (logl0n~ as x; n~ is copy number) of pGEM-16S rDNA was generated. The results of intra- and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections. 展开更多
关键词 Edwardsiella tarda TAQMAN real-time PCR detection 16S rDNA
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Road Damage Detection and Classification Using Mask R-CNN with DenseNet Backbone 被引量:3
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作者 Qiqiang Chen Xinxin Gan +2 位作者 Wei Huang Jingjing Feng H.Shim 《Computers, Materials & Continua》 SCIE EI 2020年第12期2201-2215,共15页
Automatic road damage detection using image processing is an important aspect of road maintenance.It is also a challenging problem due to the inhomogeneity of road damage and complicated background in the road images.... Automatic road damage detection using image processing is an important aspect of road maintenance.It is also a challenging problem due to the inhomogeneity of road damage and complicated background in the road images.In recent years,deep convolutional neural network based methods have been used to address the challenges of road damage detection and classification.In this paper,we propose a new approach to address those challenges.This approach uses densely connected convolution networks as the backbone of the Mask R-CNN to effectively extract image feature,a feature pyramid network for combining multiple scales features,a region proposal network to generate the road damage region,and a fully convolutional neural network to classify the road damage region and refine the region bounding box.This method can not only detect and classify the road damage,but also create a mask of the road damage.Experimental results show that the proposed approach can achieve better results compared with other existing methods. 展开更多
关键词 Road damage detection road damage classification Mask R-CNN framework densely connected network
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An Attention Based Neural Architecture for Arrhythmia Detection and Classification from ECG Signals 被引量:2
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作者 Nimmala Mangathayaru Padmaja Rani +4 位作者 Vinjamuri Janaki Kalyanapu Srinivas B.Mathura Bai G.Sai Mohan BLalith Bharadwaj 《Computers, Materials & Continua》 SCIE EI 2021年第11期2425-2443,共19页
Arrhythmia is ubiquitous worldwide and cardiologists tend to provide solutions from the recent advancements in medicine.Detecting arrhythmia from ECG signals is considered a standard approach and hence,automating this... Arrhythmia is ubiquitous worldwide and cardiologists tend to provide solutions from the recent advancements in medicine.Detecting arrhythmia from ECG signals is considered a standard approach and hence,automating this process would aid the diagnosis by providing fast,costefficient,and accurate solutions at scale.This is executed by extracting the definite properties from the individual patterns collected from Electrocardiography(ECG)signals causing arrhythmia.In this era of applied intelligence,automated detection and diagnostic solutions are widely used for their spontaneous and robust solutions.In this research,our contributions are two-fold.Firstly,the Dual-Tree Complex Wavelet Transform(DT-CWT)method is implied to overhaul shift-invariance and aids signal reconstruction to extract significant features.Next,A neural attention mechanism is implied to capture temporal patterns from the extracted features of the ECG signal to discriminate distinct classes of arrhythmia and is trained end-to-end with the finest parameters.To ensure that the model’s generalizability,a set of five traintest variants are implied.The proposed model attains the highest accuracy of 98.5%for classifying 8 variants of arrhythmia on the MIT-BIH dataset.To test the resilience of the model,the unseen(test)samples are increased by 5x and the deviation in accuracy score and MSE was 0.12%and 0.1%respectively.Further,to assess the diagnostic model performance,AUC-ROC curves are plotted.At every test level,the proposed model is capable of generalizing new samples and leverages the advantage to develop a real-world application.As a note,this research is the first attempt to provide neural attention in arrhythmia classification using MIT-BIH ECG signals data with state-of-the-art performance. 展开更多
关键词 Arrhythmia classification arrhythmia detection MIT-BIH dataset dual-tree complex wave transform ECG classification neural attention neural networks deep learning
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Real-time RT-PCR Assay for the detection of Tahyna Virus 被引量:2
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作者 LI Hao CAO Yu Xi +6 位作者 HE Xiao Xia FU Shi Hong LYU Zhi HE Ying GAO Xiao Yan LIANG Guo Dong WANG Huan Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第5期374-377,共4页
A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequ... A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance. 展开更多
关键词 PCR real-time RT-PCR Assay for the detection of Tahyna Virus TIME RT
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