Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance ...Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance thereof. With applications as exemplified by microfiuidic immunoassay, aptasensor, direct analysis in real time, high resolution mass spectrometry, benchmark dose and chemical specific adjustment factor, this review presents frontier food safety analysis and risk assessment technologies, from which both food quality and public health will benefit undoubtedly in a foreseeable future.展开更多
Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsu...Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsulated ion sources due to less efficient ion transmission.In this work,a briefly machined external ion funnel(threepole electrostatic lens)was adopted and installed between direct analysis in real time and the mass spectrometer inlet to increase detection sensitivity.Software investigation with SIMION version 8.0 was employed to calculate electric fields and to simulate the trajectories of charged particles in the electric field.Both the geometrical parameters of the ion funnel and the electric parameters were studied to obtain the optimal ion focusing results,which demonstrated the ion funnel equipment would greatly improve the ion transmission efficiency.The equipment showed good prospect in direct analysis in real time-mass spectrometry analysis in the future.展开更多
A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Saf...A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Safety Improvement Act(CPSIA),European Union legislations and Chinese national standards(GB)were analyzed.By a combined solid-phase micro-extraction(SPME)and direct analysis in real time mass spectrometry(DART-MS)approach,phthalates at sub-ng•mL^(−1)levels can be qualitatively and quantitatively analyzed in a short time.The use of ultrahigh-resolving power and the accurate mass measurement capacity naturally provided by Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS)minimizes the matrix interferences and thus enables the evaluation of phthalates in a complex matrix without extensive sample handlings or preparations.The limits of quantification(LOQs)were estimated to be at 0.3-5.0 ng•mL^(−1),lower than the Maximum Residue Limit(MRL)regulated by the European Union legislations(2007/19/EC)in foods,beverages,food packaging and toys(0.3-30 ng•mL^(−1)).This rapid and easy-to-use SPME-DART-FT-ICR-MS method provided a relatively high-throughput and powerful analytical approach for quick testing and screening phthalates in beverages and water samples to ensure food safety.展开更多
We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled w...We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled with tandem mass spectrometry(DART‑MS/MS).These samples were extracted by acetonitrile‑methanol(V/V=4:1),using DART 12 Dip‑it automatic sampling system.They were injected at 400℃,and analyzed by positive ion and multiple reaction monitoring mode.The detection limits of morphine,O6‑Monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin were 100,50,50,100,20,20,10,1,and 0.01 ng/mL,respectively.The practical cases contained methamphetamine,codeine,cocaine,and O6‑monoacetylmorphine were detected accurately and rapidly.The method has the advantages of high sensitivity and good accuracy.The sample processing is simple and can be analyzed in a short time.This method is suitable for the analysis of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in some practical cases.展开更多
以中药为代表的天然药物具有悠久的药用历史,其功效物质基础解析和质量评价对于促进中医药的传承和创新发展具有重要意义。由于中药化学组成复杂,准确分析和鉴定与临床药效密切相关的指标成分较为困难,亟需开发和运用新型分离表征手段...以中药为代表的天然药物具有悠久的药用历史,其功效物质基础解析和质量评价对于促进中医药的传承和创新发展具有重要意义。由于中药化学组成复杂,准确分析和鉴定与临床药效密切相关的指标成分较为困难,亟需开发和运用新型分离表征手段。实时直接分析质谱(direct analysis in real-time mass spectrometry,DART-MS)是近年来新兴的常压敞开式离子化质谱技术,具有分析速度快、原位、样品前处理简单等特点。自2005年被报道以来,DART-MS在生物医学、环境监测、公共安全、药物分析等领域应用广泛。该技术为解析中药复杂化学成分和质量控制提供了新方式,具有较好的应用前景。本文概述了DART-MS技术的原理、特点、影响因素及技术进展,总结其在中药分析中的应用情况,并展望其发展前景。展开更多
实时直接分析技术(direct analysis in real-time,DART)是一种在大气压环境下,利用非表面接触型热解析,对化合物进行离子化的新型软电离技术。该技术通过与样品预处理技术、质谱技术相结合,为直接、快速、无损地现场原位分析提供了一种...实时直接分析技术(direct analysis in real-time,DART)是一种在大气压环境下,利用非表面接触型热解析,对化合物进行离子化的新型软电离技术。该技术通过与样品预处理技术、质谱技术相结合,为直接、快速、无损地现场原位分析提供了一种新的途径,在农兽药残留、违禁添加成分、接触材料迁移物、生物毒素的检测方面应用广泛。该文简述实时直接分析技术的电离机理,概述该方法在常见的食品污染物检测中的应用现状和局限性,并对其未来发展方向进行展望。展开更多
该研究建立了原位电离质谱实时快速直接分析猪肉中23种兽药残留的方法,同时通过基质效应、检出限和精密度进行方法学评价。猪肉经粉碎,乙腈提取并净化,净化后采用实时直接分析离子源与三重四极杆质谱联用技术实时快速直接分析,多反应监...该研究建立了原位电离质谱实时快速直接分析猪肉中23种兽药残留的方法,同时通过基质效应、检出限和精密度进行方法学评价。猪肉经粉碎,乙腈提取并净化,净化后采用实时直接分析离子源与三重四极杆质谱联用技术实时快速直接分析,多反应监测模式检测,外标法定量。结果表明,兽药分子结构与原位电离的基质效应包含了竞争吸附和竞争离子化2个方面,喹诺酮类竞争吸附效应占据优势,磺胺硝基咪唑类竞争离子化效应占据优势;磺胺类、喹诺酮类和硝基咪唑类23种化合物在10μg/L均能够正常检出;方法精密度即相对标准偏差(relative standard deviation,RSD)为36.25%~112.36%,采用原位质谱技术检测肉品中喹诺酮类时,净化柱提取液RSD明显低于其他基质的RSD,因此在检测肉品中喹诺酮类时,采用净化柱净化,能够获得较好的精密度。对于磺胺类和硝基咪唑类,提高净化程度对精密度的改善不明显。该方法快捷、简便,准确性、灵敏度较好,适合对市售猪肉中23种兽药残留进行不合格快速筛查。展开更多
目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子...目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子化温度450℃,在正离子、多反应监测(MRM)下进行检测。结果该方法7种物质的检出限为0.025~12.5μg/g,检出阳性样品4种,分别非法添加羟基卡巴地那非、环己基去甲他达拉非、N-苄基他达拉非和N-苯基丙氧苯基卡巴地那非。结论该方法样品前处理简单,分析速度快,定性准确,环境污染小,能满足食品中非法添加PDE-5型抑制剂的快速筛查要求。展开更多
基金financially supported by the Beijing Municipal Science and Technology Project, China (Z131110000613066)the Educational and Teaching Reform Project for Graduate Students, China (G-JG-XJ201408)the Beijing Key Laboratory of Bioprocess, China
文摘Access to security and safe food is a basic human necessity and essential for a sustainable world. To perform hi-end rood safety analysis and risk assessment with state of the art technologies is of utmost importance thereof. With applications as exemplified by microfiuidic immunoassay, aptasensor, direct analysis in real time, high resolution mass spectrometry, benchmark dose and chemical specific adjustment factor, this review presents frontier food safety analysis and risk assessment technologies, from which both food quality and public health will benefit undoubtedly in a foreseeable future.
基金This work was financially supported by the National Natural Science Foundation of China(Grant Nos.21275012 and 21175008).
文摘Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsulated ion sources due to less efficient ion transmission.In this work,a briefly machined external ion funnel(threepole electrostatic lens)was adopted and installed between direct analysis in real time and the mass spectrometer inlet to increase detection sensitivity.Software investigation with SIMION version 8.0 was employed to calculate electric fields and to simulate the trajectories of charged particles in the electric field.Both the geometrical parameters of the ion funnel and the electric parameters were studied to obtain the optimal ion focusing results,which demonstrated the ion funnel equipment would greatly improve the ion transmission efficiency.The equipment showed good prospect in direct analysis in real time-mass spectrometry analysis in the future.
基金support from National Natural Science Foundation of China(Nos.21172250 and 21275155).
文摘A method for rapid identification and quantification of phthalate plasticizers in beverages was developed.A number of 15 phthalate plasticizers which covered all the phthalates concerned in the US Consumer Product Safety Improvement Act(CPSIA),European Union legislations and Chinese national standards(GB)were analyzed.By a combined solid-phase micro-extraction(SPME)and direct analysis in real time mass spectrometry(DART-MS)approach,phthalates at sub-ng•mL^(−1)levels can be qualitatively and quantitatively analyzed in a short time.The use of ultrahigh-resolving power and the accurate mass measurement capacity naturally provided by Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS)minimizes the matrix interferences and thus enables the evaluation of phthalates in a complex matrix without extensive sample handlings or preparations.The limits of quantification(LOQs)were estimated to be at 0.3-5.0 ng•mL^(−1),lower than the Maximum Residue Limit(MRL)regulated by the European Union legislations(2007/19/EC)in foods,beverages,food packaging and toys(0.3-30 ng•mL^(−1)).This rapid and easy-to-use SPME-DART-FT-ICR-MS method provided a relatively high-throughput and powerful analytical approach for quick testing and screening phthalates in beverages and water samples to ensure food safety.
基金This research was supported by the Project for Strengthening the Police Force with Science and Technology(project number 2018GABJC29).
文摘We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled with tandem mass spectrometry(DART‑MS/MS).These samples were extracted by acetonitrile‑methanol(V/V=4:1),using DART 12 Dip‑it automatic sampling system.They were injected at 400℃,and analyzed by positive ion and multiple reaction monitoring mode.The detection limits of morphine,O6‑Monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin were 100,50,50,100,20,20,10,1,and 0.01 ng/mL,respectively.The practical cases contained methamphetamine,codeine,cocaine,and O6‑monoacetylmorphine were detected accurately and rapidly.The method has the advantages of high sensitivity and good accuracy.The sample processing is simple and can be analyzed in a short time.This method is suitable for the analysis of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in some practical cases.
文摘以中药为代表的天然药物具有悠久的药用历史,其功效物质基础解析和质量评价对于促进中医药的传承和创新发展具有重要意义。由于中药化学组成复杂,准确分析和鉴定与临床药效密切相关的指标成分较为困难,亟需开发和运用新型分离表征手段。实时直接分析质谱(direct analysis in real-time mass spectrometry,DART-MS)是近年来新兴的常压敞开式离子化质谱技术,具有分析速度快、原位、样品前处理简单等特点。自2005年被报道以来,DART-MS在生物医学、环境监测、公共安全、药物分析等领域应用广泛。该技术为解析中药复杂化学成分和质量控制提供了新方式,具有较好的应用前景。本文概述了DART-MS技术的原理、特点、影响因素及技术进展,总结其在中药分析中的应用情况,并展望其发展前景。
文摘实时直接分析技术(direct analysis in real-time,DART)是一种在大气压环境下,利用非表面接触型热解析,对化合物进行离子化的新型软电离技术。该技术通过与样品预处理技术、质谱技术相结合,为直接、快速、无损地现场原位分析提供了一种新的途径,在农兽药残留、违禁添加成分、接触材料迁移物、生物毒素的检测方面应用广泛。该文简述实时直接分析技术的电离机理,概述该方法在常见的食品污染物检测中的应用现状和局限性,并对其未来发展方向进行展望。
文摘该研究建立了原位电离质谱实时快速直接分析猪肉中23种兽药残留的方法,同时通过基质效应、检出限和精密度进行方法学评价。猪肉经粉碎,乙腈提取并净化,净化后采用实时直接分析离子源与三重四极杆质谱联用技术实时快速直接分析,多反应监测模式检测,外标法定量。结果表明,兽药分子结构与原位电离的基质效应包含了竞争吸附和竞争离子化2个方面,喹诺酮类竞争吸附效应占据优势,磺胺硝基咪唑类竞争离子化效应占据优势;磺胺类、喹诺酮类和硝基咪唑类23种化合物在10μg/L均能够正常检出;方法精密度即相对标准偏差(relative standard deviation,RSD)为36.25%~112.36%,采用原位质谱技术检测肉品中喹诺酮类时,净化柱提取液RSD明显低于其他基质的RSD,因此在检测肉品中喹诺酮类时,采用净化柱净化,能够获得较好的精密度。对于磺胺类和硝基咪唑类,提高净化程度对精密度的改善不明显。该方法快捷、简便,准确性、灵敏度较好,适合对市售猪肉中23种兽药残留进行不合格快速筛查。
文摘目的将实时直接分析离子源(direct analysis in real time,DART)与三重四极杆质谱联用,建立食品中非法添加PDE-5型抑制剂的快速筛查方法。方法3种不同基质样品加入乙腈振摇10 s后,设进样速率0.2 mm/s,进样体积4μl,选择DART离子源,离子化温度450℃,在正离子、多反应监测(MRM)下进行检测。结果该方法7种物质的检出限为0.025~12.5μg/g,检出阳性样品4种,分别非法添加羟基卡巴地那非、环己基去甲他达拉非、N-苄基他达拉非和N-苯基丙氧苯基卡巴地那非。结论该方法样品前处理简单,分析速度快,定性准确,环境污染小,能满足食品中非法添加PDE-5型抑制剂的快速筛查要求。
