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Stability and accuracy of central difference method for real-time dynamic substructure testing considering mass participation coefficient
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作者 Zheng Lichang Xu Guoshan +3 位作者 Yang Ge Wang Zhen Yang Kaibo Zheng Zhenyun 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第3期625-636,共12页
For real-time dynamic substructure testing(RTDST),the influence of the inertia force of fluid specimens on the stability and accuracy of the integration algorithms has never been investigated.Therefore,this study prop... For real-time dynamic substructure testing(RTDST),the influence of the inertia force of fluid specimens on the stability and accuracy of the integration algorithms has never been investigated.Therefore,this study proposes to investigate the stability and accuracy of the central difference method(CDM)for RTDST considering the specimen mass participation coefficient.First,the theory of the CDM for RTDST is presented.Next,the stability and accuracy of the CDM for RTDST considering the specimen mass participation coefficient are investigated.Finally,numerical simulations and experimental tests are conducted for verifying the effectiveness of the method.The study indicates that the stability of the algorithm is affected by the mass participation coefficient of the specimen,and the stability limit first increases and then decreases as the mass participation coefficient increases.In most cases,the mass participation coefficient will increase the stability limit of the algorithm,but in specific circumstances,the algorithm may lose its stability.The stability and accuracy of the CDM considering the mass participation coefficient are verified by numerical simulations and experimental tests on a three-story frame structure with a tuned liquid damper. 展开更多
关键词 real-time dynamic substructure testing central difference method STABILITY mass participation coefficient tuned liquid damper
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Performance analysis of real-time and post-mission kinematic precise point positioning in marine environments
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作者 Serdar Erol Reha Metin Alkan +1 位作者 I.Murat Ozulu Veli Ilçi 《Geodesy and Geodynamics》 2020年第6期401-410,共10页
This article focuses on the performance analysis of both real-time and post-mission kinematic precise point positioning(PPP)in challenging marine environments.For this purpose,a real dynamic experiment lasting 6 h was... This article focuses on the performance analysis of both real-time and post-mission kinematic precise point positioning(PPP)in challenging marine environments.For this purpose,a real dynamic experiment lasting 6 h was carried out on a lake dam in?orum City of Turkey.While the kinematic test was continuing,the real-time PPP coordinates were obtained for each measurement epoch with a commercial real-time PPP(RT-PPP)service,namely the Trimble Center Point RTX.Then the post-mission PPP(PM-PPP)coordinates were calculated by using Multi-GNSS data and the Multi-GNSS Experiment(MGEX)precise products.The kinematic RT-PPP and PM-PPP results showed that the PPP coordinates were consistent with the relative solution at centimetre and decimetre level in horizontal and height components,respectively.This study implies that PPP technique is a powerful tool for highly accurate positioning in both real-time and post-mission modes,even for dynamic applications in harsh environments. 展开更多
关键词 Kinematic survey Precise point positioning real-time PPP IGS-RTS Global GNSS correction service
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Performance Evaluation of Three-Dimensional UWB Real-Time Locating Auto-Positioning System for Fire Rescue
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作者 Hang Yang Xunbo Li Witold Pedrycz 《Intelligent Automation & Soft Computing》 SCIE 2023年第9期3039-3058,共20页
Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on... Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on-site launching and generates real-time location data,enabling fire rescuers to arrive at the intended spot faster and correctly for effective and precise rescue.Auto-positioning with step-by-step instructions is proposed when launching the locating system,while no extra measuring instrument like Total Station(TS)is needed.Real-time location tracking is provided via a 3D space real-time locating system(RTLS)constructed using Ultra-wide Bandwidth technology(UWB),which requires electromagnetic waves to pass through concrete walls.A hybrid weighted least squares with a time difference of arrival(WLS/TDOA)positioning method is proposed to address real path-tracking issues in 3D space and to meet RTLS requirements for quick computing in real-world applications.The 3D WLS/TDOA algorithm is theoretically constructed with the Cramer-Rao lower bound(CRLB).The computing complexity is reduced to the lower bound for embedded hardware to directly compute the time differential of the arriving signals using the time-to-digital converter(TDC).The results of the experiments show that the errors are controlled when the positioning algorithm is applied in various complicated situations to fulfill the requirements of engineering applications.The statistical analysis of the data reveals that the proposed UWB RTLS auto-positioning system can track target tags with an accuracy of 0.20 m. 展开更多
关键词 3D space positioning ULTRA-WIDEBAND real-time locating system time difference of arrival Cramer-Rao lower bound fire rescue
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Real-time embedded software testing method based on extended finite state machine 被引量:6
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作者 Yongfeng Yin Bin Liu Hongying Ni 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2012年第2期276-285,共10页
The reliability of real-time embedded software directly determines the reliability of the whole real-time embedded sys- tem, and the effective software testing is an important way to ensure software quality and reliab... The reliability of real-time embedded software directly determines the reliability of the whole real-time embedded sys- tem, and the effective software testing is an important way to ensure software quality and reliability. Based on the analysis of the characteristics of real-time embedded software, the formal method is introduced into the real-time embedded software testing field and the real-time extended finite state machine (RT-EFSM) model is studied firstly. Then, the time zone division method of real-time embedded system is presented and the definition and description methods of time-constrained transition equivalence class (timeCTEC) are presented. Furthermore, the approaches of the testing sequence and test case generation are put forward. Finally, the proposed method is applied to a typical avionics real- time embedded software testing practice and the examples of the timeCTEC, testing sequences and test cases are given. With the analysis of the testing result, the application verification shows that the proposed method can effectively describe the real-time embedded software state transition characteristics and real-time requirements and play the advantages of the formal methods in accuracy, effectiveness and the automation supporting. Combined with the testing platform, the real-time, closed loop and automated simulation testing for real-time embedded software can be realized effectively. 展开更多
关键词 real-time system real-time embedded software for- mal method extended finite state machine (EFSM) testing se- quence test case.
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A Precision-Positioning Method for a High-Acceleration Low-Load Mechanism Based on Optimal Spatial and Temporal Distribution of Inertial Energy 被引量:5
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作者 Xin Chen Youdun Bai +2 位作者 Zhijun Yang Jian Gao Gongfa Chen 《Engineering》 SCIE EI 2015年第3期391-398,共8页
High-speed and precision positioning are fundamental requirements for high-acceleration low-load mechanisms in integrated circuit (IC) packaging equipment. In this paper, we derive the transient nonlinear dynamicres... High-speed and precision positioning are fundamental requirements for high-acceleration low-load mechanisms in integrated circuit (IC) packaging equipment. In this paper, we derive the transient nonlinear dynamicresponse equations of high-acceleration mechanisms, which reveal that stiffness, frequency, damping, and driving frequency are the primary factors. Therefore, we propose a new structural optimization and velocity-planning method for the precision positioning of a high-acceleration mechanism based on optimal spatial and temporal distribution of inertial energy. For structural optimization, we first reviewed the commonly flexible multibody dynamic optimization using equivalent static loads method (ESLM), and then we selected the modified ESLM for optimal spatial distribution of inertial energy; hence, not only the stiffness but also the inertia and frequency of the real modal shapes are considered. For velocity planning, we developed a new velocity-planning method based on nonlinear dynamic-response optimization with varying motion conditions. Our method was verified on a high-acceleration die bonder. The amplitude of residual vibration could be decreased by more than 20% via structural optimization and the positioning time could be reduced by more than 40% via asymmetric variable velocity planning. This method provides an effective theoretical support for the precision positioning of high-acceleration low-load mechanisms. 展开更多
关键词 high-acceleration low-load mechanism precision positioning spatial and temporal distribution inertial energy equivalent static loads method (ESLM) velocity planning
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Comparison of delay compensation methods for real-time hybrid simulation using frequency-domain evaluation index 被引量:2
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作者 Xu Weijie Guo Tong Chen Cheng 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2016年第1期129-143,共15页
The delay compensation method plays an essential role in maintaining the stability and achieving accurate real-time hybrid simulation results. The effectiveness of various compensation methods in different test scenar... The delay compensation method plays an essential role in maintaining the stability and achieving accurate real-time hybrid simulation results. The effectiveness of various compensation methods in different test scenarios, however, needs to be quantitatively evaluated. In this study, four compensation methods (i.e., the polynomial extrapolation, the linear acceleration extrapolation, the inverse compensation and the adaptive inverse compensation) are selected and compared experimentally using a frequency evaluation index (FEI) method. The effectiveness of the FEI method is first verified through comparison with the discrete transfer fimction approach for compensation methods assuming constant delay. Incomparable advantage is further demonstrated for the FEI method when applied to adaptive compensation methods, where the discrete transfer function approach is difficult to implement. Both numerical simulation and laboratory tests with predefined displacements are conducted using sinusoidal signals and random signals as inputs. Findings from numerical simulation and experimental results demonstrate that the FEI method is an efficient and effective approach to compare the performance of different compensation methods, especially for those requiring adaptation of compensation parameters. 展开更多
关键词 real-time hybrid simulation frequency domain evaluation index delay compensation methods
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Novel Real-Time Seam Tracking Algorithm Based on Vector Angle and Least Square Method 被引量:1
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作者 Guanhao Liang Qingsheng Luo +1 位作者 Zhuo Ge Xiaoqing Guan 《Journal of Beijing Institute of Technology》 EI CAS 2017年第2期150-157,共8页
Real-time seam tracking can improve welding quality and enhance welding efficiency during the welding process in automobile manufacturing.However,the teaching-playing welding process,an off-line seam tracking method,i... Real-time seam tracking can improve welding quality and enhance welding efficiency during the welding process in automobile manufacturing.However,the teaching-playing welding process,an off-line seam tracking method,is still dominant in automobile industry,which is less flexible when welding objects or situation change.A novel real-time algorithm consisting of seam detection and generation is proposed to track seam.Using captured 3D points,space vectors were created between two adjacent points along each laser line and then a vector angle based algorithm was developed to detect target points on the seam.Least square method was used to fit target points to a welding trajectory for seam tracking.Furthermore,the real-time seam tracking process was simulated in MATLAB/Simulink.The trend of joint angles vs.time was logged and a comparison between the off-line and the proposed seam tracking algorithm was conducted.Results show that the proposed real-time seam tracking algorithm can work in a real-time scenario and have high accuracy in welding point positioning. 展开更多
关键词 real-time seam tracking real-time seam detection laser scanner vector angle leastsquare method algorithm research
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Cone beam computed tomography-guided differences among registration methods for lung cancer and the effects of tumor position,treatment model,and tumor size on positioning errors 被引量:1
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作者 Jiayu Du Jie Tang +1 位作者 Qian Zhang Xiaojie Ma 《Oncology and Translational Medicine》 CAS 2021年第5期203-208,共6页
Objective To explore the differences in three different registration methods of cone beam computed tomography(CBCT)-guided down-regulated intense radiation therapy for lung cancer as well as the effects of tumor locat... Objective To explore the differences in three different registration methods of cone beam computed tomography(CBCT)-guided down-regulated intense radiation therapy for lung cancer as well as the effects of tumor location,treatment mode,and tumor size on registration.Methods This retrospective analysis included 80 lung cancer patients undergoing radiotherapy in our hospital from November 2017 to October 2019 and compared automatic bone registration,automatic grayscale(t+r)registration,and automatic grayscale(t)positioning error on the X-,Y-,and Z-axes under three types of registration methods.The patients were also grouped according to tumor position,treatment mode,and tumor size to compare positioning errors.Results On the X-,Y-,and Z-axes,automatic grayscale(t+r)and automatic grayscale(t)registration showed a better trend.Analysis of the different treatment modes showed differences in the three registration methods;however,these were not statistically significant.Analysis according to tumor sizes showed significant differences between the three registration methods(P<0.05).Analysis according to tumor positions showed differences in the X-and Y-axes that were not significant(P>0.05),while the autopsy registration in the Z-axis showed the largest difference in the mediastinal and hilar lymph nodes(P<0.05).Conclusion The treatment mode was not the main factor affecting registration error in lung cancer.Three registration methods are available for tumors in the upper and lower lungs measuring<3 cm;among these,automatic gray registration is recommended,while any gray registration method is recommended for tumors located in the mediastinal hilar site measuring<3 cm and in the upper and lower lungs≥3 cm. 展开更多
关键词 lung cancer IMRT positioning error registration method CBCT different tumor locations different treatment modes tumor size
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A Class of Real-Time Parallel Combined Methods ofDigital Simulation for Large Systems
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作者 Liu Degui & Chen Lirong(Beijing Institute of Computer Application and Simulation Technology, 100854, P. R. China) 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2000年第2期25-31,共7页
In this paper, a class of real-time parallel combined methods (RTPCM) of the digital simulation for a partitioned large system is presented. By means of combination of the parallelism across the system with the parall... In this paper, a class of real-time parallel combined methods (RTPCM) of the digital simulation for a partitioned large system is presented. By means of combination of the parallelism across the system with the parallelism across the method, stiff and non-stiff subsystems are solved in parallel on parallel computer by a parallel Rosenbrock method and a parallel RK method, respectively. Their construction, convergence and numerical stability are discussed, and the digitalsimulation experiments are conducted. 展开更多
关键词 Large system real-time parallel combined method Parallel computer
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Real-time quantitative optical method to study temperature dependence of crack propagation process in colloidal photonic crystal film
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作者 林冬风 徐余颛 +4 位作者 石将建 张瑜 罗艳红 李冬梅 孟庆波 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第7期518-523,共6页
A real-time quantitative optical method to characterize crack propagation in colloidal photonic crystal film(CPCF)is developed based on particle deformation models and previous real-time crack observations. The crac... A real-time quantitative optical method to characterize crack propagation in colloidal photonic crystal film(CPCF)is developed based on particle deformation models and previous real-time crack observations. The crack propagation process and temperature dependence of the crack propagation rate in CPCF are investigated. By this method, the crack propagation rate is found to slow down gradually to zero when cracks become more numerous and dense. Meanwhile, with the temperature increasing, the crack propagation rate constant decreases. The negative temperature dependence of the crack propagation rate is due to the increase of van der Waals attraction, which finally results in the decrease of resultant force. The findings provide new insight into the crack propagation process in CPCF. 展开更多
关键词 colloidal photonic crystal crack propagation TEMPERATURE real-time quantitative optical method
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A Novel Real-Time State-of-Health and State-of-Charge Co-Estimation Method for LiFePO_4 Battery
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作者 乔荣学 张明建 +3 位作者 刘屹东 任文举 林原 潘锋 《Chinese Physics Letters》 SCIE CAS CSCD 2016年第7期182-185,共4页
The state of charge (SOC) and state of health (SOH) are two of the most important parameters of Li-ion batteries in industrial production and in practical applications. The real-time estimation for these two param... The state of charge (SOC) and state of health (SOH) are two of the most important parameters of Li-ion batteries in industrial production and in practical applications. The real-time estimation for these two parameters is crucial to realize a safe and reliable battery application. However, this is a great problem for LiFePO4 batteries due to the large constant potential plateau in the charge/discharge process. Here we propose a combined SOC and SOH co-estimation method based on the experimental test under the simulating electric vehicle working condition. A first-order resistance-capacitance equivalent circuit is used to model the battery cell, and three parameter values, ohmic resistance (Rs), parallel resistance (Rp) and parallel capacity (Cp), are identified from a real-time experimental test. Finally we find that Rp and Cp could be utilized to make a judgement on the SOIl. More importantly, the linear relationship between Cp and the SOC is established to make the estimation of the SOC for the first time. 展开更多
关键词 of in is on SOC A Novel real-time State-of-Health and State-of-Charge Co-Estimation method for LiFePO4 Battery SOH for
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Class of modified parallel combined methods of real-time numerical simulation for a stiff system
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作者 朱珍民 刘德贵 陈丽容 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2004年第3期387-391,共5页
A class of modified parallel combined methods of real-time numerical simulation are presented for a stiff dynamic system. By combining the parallelism across the system with the parallelism across the method, and rela... A class of modified parallel combined methods of real-time numerical simulation are presented for a stiff dynamic system. By combining the parallelism across the system with the parallelism across the method, and relaxing the dependence of stage value computation on sampling time of input function, a class of modified real-time parallel combined methods are constructed. Stiff and nonstiff subsystems are solved in parallel on a parallel computer by a parallel Rosen-brock method and a parallel RK method, respectively. Their order conditions and convergences are discussed. The numerical simulation experiments show that this class of modified algorithms can get high speed and efficiency. 展开更多
关键词 stiff system real-time numerical simulation parallel combined method.
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Study on Rear-end Real-time Data Quality Control Method of Regional Automatic Weather Station
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作者 WANG Chao-qiu 《Meteorological and Environmental Research》 CAS 2011年第9期6-8,12,共4页
[Objective] The aim was to study the rear-end real-time data quality control method of regional automatic weather station. [Method] The basic content and steps of rear-end real-time data quality control of regional au... [Objective] The aim was to study the rear-end real-time data quality control method of regional automatic weather station. [Method] The basic content and steps of rear-end real-time data quality control of regional automatic weather station were introduced. Each element was treated with systematic quality control procedure. The existence of rear-end real time data of regional meteorological station in Guangxi was expounded. Combining with relevant elements and linear changes, improvement based on traditional quality control method was made. By dint of evaluation and relevant check of element, the quality of temperature and pressure was controlled. [Result] The method was optimized based on traditional quality control method, and it narrowed the effectiveness of real-time data quality control. The quality check of hourly precipitation applied relevant check of hourly minimum temperature, vertical consistency check of radar data, which can effectively improve the accuracy and credibility of hourly precipitation quality control. [Conclusion] The method was on trial for one year in the quality control of real-time data in the regional automatic meteorological station in Guangxi and had gained good outcome. 展开更多
关键词 Regional automatic stations real-time data Quality control method research China
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Label-Free and Real-Time Monitor of Binding and Dissociation Processes between Protein A and Swine IgG by Oblique-Incidence Reflectivity Difference Method
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作者 何立平 刘爽 +6 位作者 戴俊 吴琳 刘国振 韦汉福 吕惠宾 金奎娟 杨国桢 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第2期35-38,共4页
Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A (SPA) and swine immunoglobulin G (IgG), and monitor the bindings between SP... Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A (SPA) and swine immunoglobulin G (IgG), and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference (OIRD) in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics. 展开更多
关键词 SPA pH Label-Free and real-time Monitor of Binding and Dissociation Processes between Protein A and Swine IgG by Oblique-Incidence Reflectivity Difference method IGG
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A Real-Time Transient Analysis of a Functionally Graded Material Plate Using Reduced-Basis Methods
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作者 Yonghui Huang Yi Huang 《Advances in Linear Algebra & Matrix Theory》 2015年第3期98-108,共11页
Based on the hybrid numerical method (HNM) combining with a reduced-basis method (RBM), the real-time transient response of a functionally graded material (FGM) plates is obtained. The large eigenvalue problem in wave... Based on the hybrid numerical method (HNM) combining with a reduced-basis method (RBM), the real-time transient response of a functionally graded material (FGM) plates is obtained. The large eigenvalue problem in wavenumber domain has been solved through real-time off-line/on-line calculation. At off-line stage, a reduced-basis space is constructed in sample wavenumbers according to the solved eigenvalue problems. The matrices independent of parameters are projected onto the reduced-basis spaces. At on-line stage, the reduced eigenvalue problems of the arbitrary wavenumbers are built. Subsequently, the responses in wavenumber domain are obtained by the approximated eigen-pairs. Because of the application of RBM, the computational cost of transient displacement analysis of FGM plate is decreased significantly, while the accuracy of the solution and the physics of the structure are still retained. The efficiency and validity of the proposed method are demonstrated through a numerical example. 展开更多
关键词 Reduced-Basis method TRANSIENT Response Functionally GRADED Material Hybrid Numerical method real-time
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Induction of Internal Capacitance Effect in Performance Measurement of OPV (Organic Photovoltaic) Device by RTOSM (Real-Time One-Sweep Method)
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作者 Yean-San Long Shu-Tsung Hsu Teng-Chun Wu 《Journal of Energy and Power Engineering》 2014年第6期1059-1066,共8页
The advantages of OPV (organic photovoltaic) are low cost, little pollution and flexible. But challenge for OPV manufacture still is lacking of accurately performance measurement due to capacitance issue. Firstly, c... The advantages of OPV (organic photovoltaic) are low cost, little pollution and flexible. But challenge for OPV manufacture still is lacking of accurately performance measurement due to capacitance issue. Firstly, characterization of OPV requires considering the slowly temporal response due to capacitance effect, and the relative I-V (current-voltage) curves are strongly dependent on the voltage sweep direction, even for the sweep time only in few seconds or less. Secondly, the IPCE (incident photon-to-electron conversion efficiency) also shows the slowly temporal response due to capacitance effect and is dependent on the wavelength of the incident light. Furthermore, the related features for measuring I-V curves are more sensitive with temperature due to non-linear characteristics issue, but current IPCE spectra of OPV are similar to that happened in conventional crystalline Si or amorphous silicon devices. In this work, we developed a RTOSM (real-time one-sweep method) applied both in I-V and IPCE to analysis different electronic transport materials, and result showed this new approach proposed a good way to slow down testing time and having better accuracy for OPV measurement by eliminating acceptance effect instantly. 展开更多
关键词 Organic photovoltaic real-time removing capacitance effect one-sweep method.
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A rigorous real-time acoustic positioning method for ocean bottom seismic exploration
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作者 Jinye Ma Shouchuan Fang Jianhu Zhao 《Satellite Navigation》 SCIE EI CSCD 2024年第1期168-190,共23页
The conventional technique for positioning seafloor geophones in ocean bottom seismic exploration encounters several challenges,including the significant impact of outliers on positioning results,underutilization of h... The conventional technique for positioning seafloor geophones in ocean bottom seismic exploration encounters several challenges,including the significant impact of outliers on positioning results,underutilization of high-precision observations,and low efficiency in real-time data processing.These issues inevitably affect the quality of seismic exploration outcomes.To address these challenges and enhance the accuracy of geophone positioning,this paper proposes a rigorous real-time acoustic positioning method for geophones based on sequential adjustment and Baarda's outlier detection approach.The proposed method comprises three key steps:grouping the original acoustic observations,constructing the intra-group acoustic positioning model,and synthesizing the positioning results across the different groups.The validity and practicality of this approach are confirmed through a simulation experiment as well as the field experiment conducted in the Bohai Sea,China.The results demonstrate that the proposed method effectively eliminates outliers in the original observations and maximizes the utilization of high-quality observations.Compared to traditional acoustic positioning methods,it significantly reduces positioning errors from meters to decimeters,and in some cases can achieve centimeter-level precision.When the sound velocity profile in the operating sea area is measured,the method can attain the posterior standard deviation at the millimeter level and positioning errors within 10 cm.When the sound velocity profile is unknown,the method can achieve the posterior standard deviation at centimeter-level and positioning errors of approximately 20 cm. 展开更多
关键词 Ocean bottom seismic exploration Geophone positioning Acoustic positioning method Sequential adjustment Outlier detection
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Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA 被引量:9
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作者 Yan-Qin Lu Jin-Xiang Han +3 位作者 Peng Qi Wei Xu Yan-Hui Zu Bo Zhu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第45期7365-7370,共6页
AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted... AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA extraction methods including guanidinium isothiocyanate, proteinase K, NaI, NaOH lysis, alkaline lysis and simple boiling was analyzed in sample A, B and C by real-time PCR. Meanwhile, 8 clinical HBV serum samples were quantified. RESULTS: The copy number of the same HBV serum sample originated from the standard curve of S, C and X regions was 5.7 × 10^4/mL, 6.3 × 10^2/mL and 1.6 × 10^3/ mL respectively. The relative Ct value was 26.6, 31.8 and 29.5 respectively. Therefore, primers and matched probe from S region were chosen for further optimization of six extraction methods. The copy number of HBV serum samples A, B and C was 3.49 × 10^9/mL, 2.08 × 10^6/mL and 4.40 × 10^7/mL respectively, the relative Ct value was 19.9, 30 and 26.2 in the method of NaOH lysis, which was the efficientest among six methods. Simple boiling showed a slightly lower efficiency than NaOH lysis. Guanidinium isothiocyanate, proteinase K and NaI displayed that the copy number of HBV serum sample A, B and C was around 10^S/mL, meanwhile the Ct value was about 30. Alkaline failed to quantify the copy number of three HBV serum samples. Standard deviation (SD) and coefficient variation (CV) were very low in all 8 clinical HBV serum samples, showing that quantification of HBV DNA in triplicate was reliable and accurate. CONCLUSION: Real-time PCR based on optimized primers and TaqMan probe from S region in combination with NaOH lysis is a simple, rapid and accurate method for quantification of HBV serum DNA. 展开更多
关键词 Hepatitis B virus Serum DNA real-time PCR Extraction method
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FEM model for real-time guide wire simulation in vasculature
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作者 张秋葵 Pascal Haigron +1 位作者 罗立民 舒华忠 《Journal of Southeast University(English Edition)》 EI CAS 2008年第1期50-54,共5页
A model suitable for describing the mechanical response of thin elastic objects is proposed to simulate the deformation of guide wires in minimally invasive interventions. The main objective of this simulation is to p... A model suitable for describing the mechanical response of thin elastic objects is proposed to simulate the deformation of guide wires in minimally invasive interventions. The main objective of this simulation is to provide doctors an opportunity to rehearse the surgery and select an optimal operation plan before the real surgery. In this model the guide wire is discretized with the multi-body representation and its elastic energy derivate from elastic theory is a polynomial function of the nodal displacements. The vascular structure is represented by a tetrahedron mesh extended from the triangular mesh of the artery, which can be extracted from the patient's CT image data. The model applies the energy decline process of the conjugate gradient method to the deformation simulation of the guide wire. Experimental results show that the polynomial relationship between elastic energy and nodal displacements tremendously simplifies the evaluation of the conjugate gradient method and significantly improves the model's efficiency. Compared with models depending on an explicit scheme for evaluation, the new model is not only non-conditionally stable but also more efficient. The model can be applied to the real-time simulation of guide wire in a vascular structure. 展开更多
关键词 deformable model finite element method real-time simulation guide wire
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One Step Positive Approach for Sheet Metal Forming Simulation Based on Quasi-conjugate-gradient Method 被引量:3
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作者 NA Jingxin CHEN Wei 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2013年第4期730-736,共7页
In one step inverse finite element approach, an initial blank shape is normally predicted from the final deformed shape. The final deformed shape needs to be trimmed into a final part after stamping, the trimmed area,... In one step inverse finite element approach, an initial blank shape is normally predicted from the final deformed shape. The final deformed shape needs to be trimmed into a final part after stamping, the trimmed area, therefore, needs to be compensated manually before using one step inverse approach, which causes low efficiency and in consistency with the real situation. To solve this problem, one step positive approach is proposed to simulate the sheet metal stamping process. Firstly the spatial initial solution of one step positive method is preliminarily obtained by using the mapping relationship and area coordinates, then based on the deformation theory the iterative solving is carried out in three-dimensional coordinate system by using quasi-conjugate-gradient method. During iterative process the contact judgment method is introduced to ensure that the nodes on the spatial initial solution are not separated from die surface. The predicted results of sheet metal forming process that include the shape and thickness of the stamped part can be obtained after the iterative solving process. The validity of the proposed approach is verified by comparing the predicted results obtained through the proposed approach with those obtained through the module of one step inverse approach in Autoform and the real stamped part. In one step positive method, the stamped shape of regular sheet can be calculated fast and effectively. During the iterative solution, the quasi-conjugate-gradient method is proposed to take the place of solving system of equations, and it can improve the stability and precision of the algorithm. 展开更多
关键词 positive approach deformation theory quasi-conjugate-gradient method
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