Molecular diagnosis and direct quantification of cereal cyst nematode(Heterodera filipjevi) from field soil using TaqMan real-time PCR

Heterodera filipjevi continues to be a major threat to wheat production worldwide.Rapid detection and quantification of cyst nematodes are essential for more effective control against this nematode disease.In the present study,a TaqManminor groove binder(TaqMan-MGB)probe-based fluorescence quantitative real-time PCR(qPCR)was successfully developed and used for quantifying H.filipjevi from DNA extracts of soil.The primers and probe designed from the obtained RAPD-SCAR marker fragments of H.filipjevi showed high specificity to H.filipjevi using DNA from isolatesconfirmed species of 23 Heterodera spp.,1 Globodera spp.and 3 Pratylenchus spp.The qPCR assay is highly sensitive and provides improved H.filipjevi detection sensitivity of as low as 4^(-3) single second-stage juvenile(J2)DNAs,10^(-3) female DNAs,and 0.01μgμL^(-1) genomic DNAs.A standard curve relating to the threshold cycle and log values of nematode numbers was generated and validated from artificially infested soils and was used to quantify H.filipjevi in naturally infested field soils.There was a high correlation between the H.filipjevi numbers estimated from 32 naturally infested field soils by both conventional methods and the numbers quantified using the qPCR assay.qPCR potentially provides a useful platform for the efficient detection and quantification of H.filipjevi directly from field soils and to quantify this species directly from DNA extracts of field soils.

运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型

运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性弧菌生长预测模型,并与传统涂布计数方法进行比较。结果表明,Real-time qPCR方法和传统计数方法均可建立Gmopertz模型、Logistic模型和Richards模型,模型拟合的相关系数R2均在0.9以上。基于Real-timeqPCR方法省时省力、特异性好等优点,用Real-time qPCR方法建立微生物预测模型是未来预测微生物学领域的一种发展趋势。

REAL-TIME TRACKING FOR FAST MOVING OBJECT ON COMPLEX BACKGROUND

A real-time tracking system for the fast moving object on the complex background is proposed.The Markov random filed（MRF）model based background subtraction algorithm is used to detect the changing pixels and track the moving object.The prior probability of the segmentation mask is modeled by using MRF,and the object tracking task is translated into the maximum a-posterior（MAP）problem.Experimental results show that the method is efficient at both offline and online moving objects on simple and complex background.

Real-Time PCR Technique and Its Application in Quantification of Plant Nucleic Acid Molecules

Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.

Exploration of instantaneous frequency for local control assessment in real-time hybrid simulation

Local control parameters such as instantaneous delay and instantaneous amplitude play an essential role in evaluating the performance and maintaining the stability of real-time hybrid simulation(RTHS).However,existing methods have limitations in obtaining this local assessment in either the time domain or frequency domain.In this study,the instantaneous frequency is introduced to determine local control parameters for actuator tracking assessment in a real-time hybrid simulation.Instantaneous properties,including amplitude,delay,frequency and phase,are then calculated based on analytic signals translated from actuator tracking signals through the Hilbert transform.Potential issues are discussed and solutions are proposed for calculation of local control parameters.Numerical simulations are first conducted for sinusoidal and chirp signals with time varying amplitude error and delay to demonstrate the potential of the proposed method.Laboratory tests also are conducted for a predefined random signal as well as the RTHS of a single degree of freedom structure with a self-centering viscous damper to experimentally verify the effectiveness of the proposed use of the instantaneous frequency.Results from the ensuing analysis clearly demonstrate that the instantaneous frequency provides great potential for local control assessment,and the proposed method enables local tracking parameters with good accuracy.

Analysis of actuator delay and its effect on uncertainty quantification for real-time hybrid simulation

Uncertainties in structure properties can result in different responses in hybrid simulations. Quantification of the effect of these tmcertainties would enable researchers to estimate the variances of structural responses observed from experiments. This poses challenges for real-time hybrid simulation （RTHS） due to the existence of actuator delay. Polynomial chaos expansion （PCE） projects the model outputs on a basis of orthogonal stochastic polynomials to account for influences of model uncertainties. In this paper, PCE is utilized to evaluate effect of actuator delay on the maximum displacement from real-time hybrid simulation of a single degree of freedom （SDOF） structure when accounting for uncertainties in structural properties. The PCE is first applied for RTHS without delay to determine the order of PCE, the number of sample points as well as the method for coefficients calculation. The PCE is then applied to RTHS with actuator delay. The mean, variance and Sobol indices are compared and discussed to evaluate the effects of actuator delay on uncertainty quantification for RTHS. Results show that the mean and the variance of the maximum displacement increase linearly and exponentially with respect to actuator delay, respectively. Sensitivity analysis through Sobol indices also indicates the influence of the single random variable decreases while the coupling effect increases with the increase of actuator delay.

Novel Real-Time Seam Tracking Algorithm Based on Vector Angle and Least Square Method

Real-time seam tracking can improve welding quality and enhance welding efficiency during the welding process in automobile manufacturing.However,the teaching-playing welding process,an off-line seam tracking method,is still dominant in automobile industry,which is less flexible when welding objects or situation change.A novel real-time algorithm consisting of seam detection and generation is proposed to track seam.Using captured 3D points,space vectors were created between two adjacent points along each laser line and then a vector angle based algorithm was developed to detect target points on the seam.Least square method was used to fit target points to a welding trajectory for seam tracking.Furthermore,the real-time seam tracking process was simulated in MATLAB/Simulink.The trend of joint angles vs.time was logged and a comparison between the off-line and the proposed seam tracking algorithm was conducted.Results show that the proposed real-time seam tracking algorithm can work in a real-time scenario and have high accuracy in welding point positioning.

A machine vision approach to seam tracking in real-time in PAW of large-diameter stainless steel tube

Manual monitoring and seam tracking through watching weld pool images in real-time, by naked eyes or by industrial TV, are experience-depended, subjective, labor intensive, and sometimes biased. So it is necessary to realize the automation of computer-aided seam tracking. A PAW (plasma arc welding) seam tracking system was developed, which senses the molten pool and the seam in one frame by a vision sensor, and then detects the seam deviation to adjust the work piece motion adaptively to the seam position sensed by vision sensor. A novel molten pool area image-processing algorithm based on machine vision was proposed. The algorithm processes each image at the speed of 20 frames/second in real-time to extract three feature variables to get the seam deviation. It is proved experimentally that the algorithm is very fast and effective. Issues related to the algorithm are also discussed.

Real-Time Front Vehicle Detection Algorithm Based on Local Feature Tracking Method

This paper proposes an algorithm that extracts features of back side of the vehicle and detects the front vehicle in real-time by local feature tracking of vehicle in the continuous images.The features in back side of the vehicle are vertical and horizontal edges,shadow and symmetry.By comparing local features using the fixed window size,the features in the continuous images are tracked.A robust and fast Haarlike mask is used for detecting vertical and horizontal edges,and shadow is extracted by histogram equalization,and the sliding window method is used to compare both side templates of the detected candidates for extracting symmetry.The features for tracking are vertical edges,and histogram is used to compare location of the peak and magnitude of the edges.The method using local feature tracking in the continuous images is more robust for detecting vehicle than the method using single image,and the proposed algorithm is evaluated by continuous images obtained on the expressway and downtown.And it can be performed on real-time through applying it to the embedded system.

Real-time accurate hand path tracking and joint trajectory planning for industrial robots(Ⅰ)

Previously, researchers raised the accuracy for a robot′s hand to track a specified path in Car-tesian space mainly through increasing the number of knots on the path and the number of the path′s segments, which results in the heavier online computational burden for the robot controller. Aiming at overcoming this drawback, the authors propose a new kind of real-time accurate hand path tracking and joint trajectory planning method. Through selecting some extra knots on the specified hand path by a certain rule and introducing a sinusoidal function to the joint displacement equation of each segment, this method can greatly raise the path tracking accuracy of robot′s hand and does not change the number of the path′s segments. It also does not increase markedly the computational burden of robot controller. The result of simulation indicates that this method is very effective, and has important value in increasing the application of industrial robots.

A Real-time Face/Hand Tracking Method for Chinese Sign Language Recognition

This paper introduces a new Chinese Sign Language recognition (CSLR) system and a method of real time tracking face and hand applied in the system. In the method, an improved agent algorithm is used to extract the region of face and hand and track them. Kalman filter is introduced to forecast the position and rectangle of search, and self adapting of target color is designed to counteract the effect of illumination.

Real-time accurate hand path tracking and joint trajectory planning for industrial robots(Ⅱ)

Previously, researchers raised the accuracy for a robot′s hand to track a specified path in Cartesian space mainly through increasing the number of knots on the path and the segments of the path. But, this method resulted in the heavier on line computational burden for the robot controller. In this paper, aiming at this drawback, the authors propose a new kind of real time accurate hand path tracking and joint trajectory planning method for robots. Through selecting some extra knots on the specified hand path by a certain rule, which enables the number of knots on each segment to increase from two to four, and through introducing a sinusoidal function and a cosinoidal function to the joint displacement equation of each segment, this method can raise the path tracking accuracy of robot′s hand greatly but does not increase the computational burden of robot controller markedly.

Real-time tracking of deformable objects based on MOK algorithm

The traditional oriented FAST and rotated BRIEF（ORB） algorithm has problems of instability and repetition of keypoints and it does not possess scale invariance. In order to deal with these drawbacks, a modified ORB（MORB） algorithm is proposed. In order to improve the precision of matching and tracking, this paper puts forward an MOK algorithm that fuses MORB and Kanade-Lucas-Tomasi（KLT）. By using Kalman, the object＇s state in the next frame is predicted in order to reduce the size of search window and improve the real-time performance of object tracking. The experimental results show that the MOK algorithm can accurately track objects with deformation or with background clutters, exhibiting higher robustness and accuracy on diverse datasets. Also, the MOK algorithm has a good real-time performance with the average frame rate reaching 90.8 fps.

Real-time localization for underwater equipment using an extremely low frequency electric field

A new real-time underwater equipment location method adopting an electric field induced by a standard current source is proposed.Our goals were real-time tracking and location of stationary or moving underwater equipment both in shallow and deep seas,under noisy conditions.The main features of this method are as follows:(1)a standard current source on the water surface,which can be towed by a vehicle,consisting of two electrodes,a signal generator,and a GPS unit;(2)measurement of the extremely low frequency(ELF)electric field emitted by the current source,made possible by electric field sensors on the underwater equipment;(3)position of the underwater equipment is estimated in real time based on a progressive update extended Kalman filter(PUEKF),which is carried out using the propagation model of an ELF electric field because the electric field at the position of the underwater equipment and the current source position are known.We verified the accuracy of our method and confirmed real-time location feasibility through numerical,physical scale,and real-time sea experiments.Through numerical experiments,we verified that our method works for underwater equipment location in real-world conditions,and the location error can be less than 0.2 m.Next,real-time location experiments for stationary underwater measuring equipment in water tank were conducted.The result shows that the location error can be less than 0.1 m.We also confirmed real-time location feasibility through the use of offshore experiment.We expect that our method will complement conventional underwater acoustic location methods for underwater equipment in acoustically noisy environments.

Multiple-Object Tracking Using Histogram Stamp Extraction in CCTV Environments

Object tracking,an important technology in the field of image processing and computer vision,is used to continuously track a specific object or person in an image.This technology may be effective in identifying the same person within one image,but it has limitations in handling multiple images owing to the difficulty in identifying whether the object appearing in other images is the same.When tracking the same object using two or more images,there must be a way to determine that objects existing in different images are the same object.Therefore,this paper attempts to determine the same object present in different images using color information among the unique information of the object.Thus,this study proposes a multiple-object-tracking method using histogram stamp extraction in closed-circuit television applications.The proposed method determines the presence or absence of a target object in an image by comparing the similarity between the image containing the target object and other images.To this end,a unique color value of the target object is extracted based on its color distribution in the image using three methods:mean,mode,and interquartile range.The Top-N accuracy method is used to analyze the accuracy of each method,and the results show that the mean method had an accuracy of 93.5%(Top-2).Furthermore,the positive prediction value experimental results show that the accuracy of the mean method was 65.7%.As a result of the analysis,it is possible to detect and track the same object present in different images using the unique color of the object.Through the results,it is possible to track the same object that can minimize manpower without using personal information when detecting objects in different images.In the last response speed experiment,it was shown that when the mean was used,the color extraction of the object was possible in real time with 0.016954 s.Through this,it is possible to detect and track the same object in real time when using the proposed method.

High-speed railway track components inspection framework based on YOLOv8 with high-performance model deployment

Railway inspection poses significant challenges due to the extensive use of various components in vast railway networks,especially in the case of high-speed railways.These networks demand high maintenance but offer only limited inspection windows.In response,this study focuses on developing a high-performance rail inspection system tailored for high-speed railways and railroads with constrained inspection timeframes.This system leverages the latest artificial intelligence advancements,incorporating YOLOv8 for detection.Our research introduces an efficient model inference pipeline based on a producer-consumer model,effectively utilizing parallel processing and concurrent computing to enhance performance.The deployment of this pipeline,implemented using C++,TensorRT,float16 quantization,and oneTBB,represents a significant departure from traditional sequential processing methods.The results are remarkable,showcasing a substantial increase in processing speed:from 38.93 Frames Per Second(FPS)to 281.06 FPS on a desktop system equipped with an Nvidia RTX A6000 GPU and from 19.50 FPS to 200.26 FPS on the Nvidia Jetson AGX Orin edge computing platform.This proposed framework has the potential to meet the real-time inspection requirements of high-speed railways.

RFID Based e-quality Tracking in Service-oriented Manufacturing Execution System

The method of acquiring the real-time data has influenced the implementation of the manufacturing execution system （MES）. Accompanied with turning the MES into service-oriented manufacturing execution system （so-MES）, real-time e-quality tracking （e-QT）, in which real-time data are computed, has played more and more important roles in manufacturing. This paper presents an e-QT model through the study of real-time status data tracking and quality data collecting. An implementing architecture of the e-QT model is constructed on the basis of radio frequency identification devices （RFID） data-tracking network. In order to develop the e-QT system, some key enabling technologies, such as configuration, data collection, and data processing, etc, are studied. The relation schema between hardware is built for the RFID data-tracking network based on the configuration technique. Real-time data are sampled by using data collecting technique. Furthermore, real-time status and quality data in a shop-floor can be acquired in terms of using the real-time data computing method. Finally, a prototype system is developed and a running example is given so as to verify the feasibility of methods proposed in this paper. The proposed research provides effective e-quality tracking theoretical foundation through the use of RFID technology for the discrete manufacturing.

Current trends in three-dimensional visualization and real-time navigation as well as robot-assisted technologies in hepatobiliary surgery

With the continuous development of digital medicine,minimally invasive precision and safety have become the primary development trends in hepatobiliary surgery.Due to the specificity and complexity of hepatobiliary surgery,traditional preoperative imaging techniques such as computed tomography and magnetic resonance imaging cannot meet the need for identification of fine anatomical regions.Imaging-based three-dimensional(3D)reconstruction,virtual simulation of surgery and 3D printing optimize the surgical plan through preoperative assessment,improving the controllability and safety of intraoperative operations,and in difficult-to-reach areas of the posterior and superior liver,assistive robots reproduce the surgeon’s natural movements with stable cameras,reducing natural vibrations.Electromagnetic navigation in abdominal surgery solves the problem of conventional surgery still relying on direct visual observation or preoperative image assessment.We summarize and compare these recent trends in digital medical solutions for the future development and refinement of digital medicine in hepatobiliary surgery.

A Real-Time System for Monitoring Distant Dynamic Displacement of Structures

Based on digital image processing technique, a real-time system is developed to monitor and detect the dynamic displacement of engineering structures. By processing pictures with a self-programmed software, the real-time coordinate of an object in a certain coordinate system can be obtained, and further dynamic displacement data and curve of the object can also be achieved. That is, automatic gathering and real-time processing of data can be carried out by this system simultaneously. For this system, first, an untouched monitoring technique is adopted, which can monitor or detect objects several to hundreds of meters apart; second, it has flexible installation condition and good monitoring precision of sub-millimeter degree; third, it is fit for dynamic, quasi-dynamic and static monitoring of large engineering structures. Through several tests and applications in large bridges, good reliability and dominance of the system is proved.

A STUDY ON THE ENSEMBLE FORECAST REAL-TIME CORRECTION METHOD

Using real-time correction technology for typhoons, this paper discusses real-time correction for forecasting the track of four typhoons during 2009 and 2010 in Japan, Beijing, Guangzhou, and Shanghai. It was determined that the short-time forecast effect was better than the original objective mode. By selecting four types of integration schemes after multiple mode path integration for those four objective modes, the forecast effect of the multi-mode path integration is better, on average, than any single model. Moreover, multi-mode ensemble forecasting has obvious advantages during the initial 36 h.