目的基于术前血清IL-6、前列腺素E2(PGE2)、TNF-α构建预测膀胱癌术后复发的列线图模型。方法回顾性收集2018年6月至2023年2月临平区第一人民医院收治的348例膀胱癌患者的临床资料,经计算机产生随机数表并以2∶1比例将其分为训练集(232...目的基于术前血清IL-6、前列腺素E2(PGE2)、TNF-α构建预测膀胱癌术后复发的列线图模型。方法回顾性收集2018年6月至2023年2月临平区第一人民医院收治的348例膀胱癌患者的临床资料,经计算机产生随机数表并以2∶1比例将其分为训练集(232例)和验证集(116例)。所有患者均接受随访,将发生复发的患者纳入复发组,未发生复发的患者纳入未复发组。比较训练集复发组、未复发组血清IL-6、PGE2、TNF-α水平及一般资料;用Logistic回归模型分析训练集膀胱癌术后复发的影响因素,并建立回归方程;用ROC曲线分析术前IL-6、PGE2、TNF-α单独及联合预测膀胱癌术后复发的效能;建立膀胱癌术后复发的风险预测列线图模型,并验证其效能。结果与未复发组比较,复发组血清IL-6、PGE2、TNF-α水平升高,肿瘤直径增大,多发性肿瘤、肿瘤分期T 2~T 4、肿瘤WHO病理学分级Ⅱ~Ⅲ级的构成比升高,术后规律膀胱灌注的构成比降低(P<0.05)。Logistic回归分析显示,术前血清IL-6、PGE2、TNF-α、肿瘤分期、肿瘤WHO病理学分级是膀胱癌术后复发的影响因素(P<0.05),并建立Logistic回归方程:Y=1.718 X 1+2.081 X 2+1.815 X 3+2.319 X 4+1.868 X 5。ROC曲线显示,术前IL-6、PGE2、TNF-α预测膀胱癌术后复发的最佳截断点分别为0.60 ng/L、57.13 pg/mL、2.10 ng/mL,三者单独及联合预测膀胱癌的ROC曲线下面积(AUC ROC)分别为0.729、0.743、0.733和0.825。基于训练集Logistic回归分析结构建立膀胱癌术后复发的风险预测列线图模型,该模型预测训练集、验证集的敏感性分别为94.12%、90.20%,特异性分别为90.06%、87.29%,AUC ROC分别为0.940、0.914;Bootstrap法内部验证结果显示,训练集、验证集的C-index分别为0.918(95%CI:0.824~0.987)、0.901(95%CI:0.835~0.957)。结论术前血清IL-6、PGE2、TNF-α水平是膀胱癌术后复发的影响因素,据此建立的风险预测列线图模型具有良好的预测效能。展开更多
Objective: To investigate the effects of Yanghe Pingchuan Granules on airway remodeling in asthmatic rats, and to explore the mechanism of Interleukin-6/Janus kinase 2/ Signal transducing activator of transcription 3(...Objective: To investigate the effects of Yanghe Pingchuan Granules on airway remodeling in asthmatic rats, and to explore the mechanism of Interleukin-6/Janus kinase 2/ Signal transducing activator of transcription 3(IL-6/JAK2/STAT3) signal axis. Methods: We separated 42 healthy male SD rats into two groups, a control group (7) and a model group (35).The model group was sensitized with a combination of ovalbumin (OVA) and aluminum hydroxide for 2 weeks, while the control group was given an equal amount of physiological saline.After 2 weeks, the modeling group was randomly divided into Model group, Yanghe Pingchuan Granules high, medium and low dose groups and Dexamethasone group, each group consisted of 7 animals. After 4 weeks, OVA atomization and gavage were used for stimulation and treatment. Yanghe Pingchuan Granules high, middle and low groups were given 15.48, 7.74, 3.87 g∙kg-1 Yanghe Pingchuan Granules daily, dexamethasone group was given 0.0625 mg∙kg-1 dexamethasone daily, and the other groups were given the same amount of normal saline. HE, PAS and Masson staining were used to observe the lung histopathological changes in rats. The levels of interleukin-6, IL-23 and IL-17A were detected by ELISA. The expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 in lung tissues were detected by Western blot. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression levels of IL-6, JAK2 and STAT3 in rat lung tissue. Results: The lung tissue structure of the model group was severely damaged compared to the control group, accompanied by a great many of inflammatory cell infiltration, goblet cell hyperplasia, subepithelial collagen fiber deposition and airway epithelial thickening were more obvious. The expressions of IL-6, IL- 23 and IL-17A in serum were significantly increased (P<0.01), the protein expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 and the mRNA expression levels of IL-6, JAK2 and STAT3 in lung tissue were significantly increased (P<0.01);Compared with the model group, inflammatory cell infiltration, goblet cell proliferation, subepithelial collagen fiber deposition and airway epithelial thickening were significantly reduced in each administration group, and the expressions of IL-6, IL-23 and IL-17A in serum were significantly decreased (P< 0.01). The protein expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 and mRNA expression levels of IL-6, JAK2 and STAT3 in lung tissue were significantly decreased (P<0.01). Conclusion: Yanghe Pingchuan Granules can significantly alleviate airway remodeling in asthmatic rats, and its mechanism may be through inhibiting the IL-6/JAK2/STAT3 signal axis.展开更多
文摘目的基于术前血清IL-6、前列腺素E2(PGE2)、TNF-α构建预测膀胱癌术后复发的列线图模型。方法回顾性收集2018年6月至2023年2月临平区第一人民医院收治的348例膀胱癌患者的临床资料,经计算机产生随机数表并以2∶1比例将其分为训练集(232例)和验证集(116例)。所有患者均接受随访,将发生复发的患者纳入复发组,未发生复发的患者纳入未复发组。比较训练集复发组、未复发组血清IL-6、PGE2、TNF-α水平及一般资料;用Logistic回归模型分析训练集膀胱癌术后复发的影响因素,并建立回归方程;用ROC曲线分析术前IL-6、PGE2、TNF-α单独及联合预测膀胱癌术后复发的效能;建立膀胱癌术后复发的风险预测列线图模型,并验证其效能。结果与未复发组比较,复发组血清IL-6、PGE2、TNF-α水平升高,肿瘤直径增大,多发性肿瘤、肿瘤分期T 2~T 4、肿瘤WHO病理学分级Ⅱ~Ⅲ级的构成比升高,术后规律膀胱灌注的构成比降低(P<0.05)。Logistic回归分析显示,术前血清IL-6、PGE2、TNF-α、肿瘤分期、肿瘤WHO病理学分级是膀胱癌术后复发的影响因素(P<0.05),并建立Logistic回归方程:Y=1.718 X 1+2.081 X 2+1.815 X 3+2.319 X 4+1.868 X 5。ROC曲线显示,术前IL-6、PGE2、TNF-α预测膀胱癌术后复发的最佳截断点分别为0.60 ng/L、57.13 pg/mL、2.10 ng/mL,三者单独及联合预测膀胱癌的ROC曲线下面积(AUC ROC)分别为0.729、0.743、0.733和0.825。基于训练集Logistic回归分析结构建立膀胱癌术后复发的风险预测列线图模型,该模型预测训练集、验证集的敏感性分别为94.12%、90.20%,特异性分别为90.06%、87.29%,AUC ROC分别为0.940、0.914;Bootstrap法内部验证结果显示,训练集、验证集的C-index分别为0.918(95%CI:0.824~0.987)、0.901(95%CI:0.835~0.957)。结论术前血清IL-6、PGE2、TNF-α水平是膀胱癌术后复发的影响因素,据此建立的风险预测列线图模型具有良好的预测效能。
基金The Sixth Batch of Special Support Plans in Anhui Province(No.dlPtzjh20200050)Key Natural Science Research Project of Higher Education Institutions in Anhui Province(No.KJ2020A0426)。
文摘Objective: To investigate the effects of Yanghe Pingchuan Granules on airway remodeling in asthmatic rats, and to explore the mechanism of Interleukin-6/Janus kinase 2/ Signal transducing activator of transcription 3(IL-6/JAK2/STAT3) signal axis. Methods: We separated 42 healthy male SD rats into two groups, a control group (7) and a model group (35).The model group was sensitized with a combination of ovalbumin (OVA) and aluminum hydroxide for 2 weeks, while the control group was given an equal amount of physiological saline.After 2 weeks, the modeling group was randomly divided into Model group, Yanghe Pingchuan Granules high, medium and low dose groups and Dexamethasone group, each group consisted of 7 animals. After 4 weeks, OVA atomization and gavage were used for stimulation and treatment. Yanghe Pingchuan Granules high, middle and low groups were given 15.48, 7.74, 3.87 g∙kg-1 Yanghe Pingchuan Granules daily, dexamethasone group was given 0.0625 mg∙kg-1 dexamethasone daily, and the other groups were given the same amount of normal saline. HE, PAS and Masson staining were used to observe the lung histopathological changes in rats. The levels of interleukin-6, IL-23 and IL-17A were detected by ELISA. The expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 in lung tissues were detected by Western blot. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression levels of IL-6, JAK2 and STAT3 in rat lung tissue. Results: The lung tissue structure of the model group was severely damaged compared to the control group, accompanied by a great many of inflammatory cell infiltration, goblet cell hyperplasia, subepithelial collagen fiber deposition and airway epithelial thickening were more obvious. The expressions of IL-6, IL- 23 and IL-17A in serum were significantly increased (P<0.01), the protein expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 and the mRNA expression levels of IL-6, JAK2 and STAT3 in lung tissue were significantly increased (P<0.01);Compared with the model group, inflammatory cell infiltration, goblet cell proliferation, subepithelial collagen fiber deposition and airway epithelial thickening were significantly reduced in each administration group, and the expressions of IL-6, IL-23 and IL-17A in serum were significantly decreased (P< 0.01). The protein expression levels of JAK-2, P-JAK2, STAT3 and P-STAT3 and mRNA expression levels of IL-6, JAK2 and STAT3 in lung tissue were significantly decreased (P<0.01). Conclusion: Yanghe Pingchuan Granules can significantly alleviate airway remodeling in asthmatic rats, and its mechanism may be through inhibiting the IL-6/JAK2/STAT3 signal axis.
