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Low-Temperature Response to Major Agronomic Traits by Using Recombinant Inbred Line(RIL) Populations Derived from Towada × Kunmingxiaobaigu 被引量:1
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作者 XU Fu-rong YU Teng-qiong TANG Cui-feng A Xin-xiang FAN Chuan-zhang HU Yi-liang ZHANG Dun-yu DONG Chao DAI Lu-yuan 《Agricultural Sciences in China》 CAS CSCD 2009年第11期1301-1311,共11页
Development of the recombinant inbred line populations (RILs) is important basis to detect QTLs for cold tolerance at booting stage in rice. A set of 230 RILs derived from the cross of Towada and Kunmingxiaobaigu we... Development of the recombinant inbred line populations (RILs) is important basis to detect QTLs for cold tolerance at booting stage in rice. A set of 230 RILs derived from the cross of Towada and Kunmingxiaobaigu were used for evaluation of low-temperature response on major agronomic traits of plant height (PH), panicle length (PL), panicle exsertion (PE), spikelet fertility (SF), specific spikelet fertility (SSF), and spikelets per panicle (SPP) under natural low-temperature growing environments in Yunnan Province, China. The results showed PH, PE, and SPP were mainly attributed by genotypes. PL was mainly influenced interactively by the genotypes × environments. SF and SSF were mainly controlled by the environments. Under the five different growth environments, F values of the six agronomic traits mentioned above ranged from 4.019 to 97.284. Significant difference was revealed between the lines. Under every environment, it indicated significantly positive correlation between SF and SSF, with correlation coefficients ranged from 0.826 to 0.885. It indicated significantly positive correlation between PH, PL, and PE. Under five different growing environments, variation coefficients of the six characters ordered in SSF (66.3%) 〉 PE (57.4%) 〉 SP (37.2%) 〉 SPP (16.2%) 〉 PH (9.6%) 〉 PL (6.4%). SSF, PE and SF were most sensitive to low temperature stress at booting stage, while SPP, PH and PL being least. The RILs of Towada/ Kunmingxiaobaigu can be used as a genetic population to investigate cold tolerance at booting stage. SSF, PE and SF are most sensitive to cold tolerance at booting stage in rice. So far the the variation of PH, PL, and SPP related to cold tolerance are not clear under natural low-temperature environment. More tested environments and years are required to identify and evaluate cold tolerance at booting stage in rice. 展开更多
关键词 ANOVA correlation analysis low-temperature response major agronomic traits recombination inbred line population ril Oryza sativa L. sp. japonica
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Combining Ability Analysis on Yield Traits of Recombinant Inbred Lines in Hybrid Rice
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作者 曹应江 游书梅 +8 位作者 郑家奎 蒋开锋 张涛 杨莉 杨乾华 万先齐 罗婧 李昭祥 高磊 《Agricultural Science & Technology》 CAS 2015年第9期1877-1882,共6页
A population of 140 recombinant inbred lines at F8 generation were obtained after seven successive generations of self-pollination using single seed descent(SSD) method from the F2 hybrids of three-line restorers Lu... A population of 140 recombinant inbred lines at F8 generation were obtained after seven successive generations of self-pollination using single seed descent(SSD) method from the F2 hybrids of three-line restorers Luhui 8258 with high combining ability and Yanghui 34. Then, the 140 inbred lines obtained above and their parents Luhui 8258 and Yanghui 34 were crossed with three different types of cyto-plasmic male sterile(CMS) lines(Gang 46 A, Ⅱ-32 A and Lu 98A) according to NCⅡ design. The resulting 426 combinations were planted at Deyang and Suining bases to test the combining ability and inheritance of five yield traits: yield per plant, panicle number per plant, filled grain number per panicle, seed setting rate and 1 000-grain weight. The results showed that the variances of both general and specific combining abilities of the five traits all reached a significant or extremely significant level at the two sites. The broad and narrow heritability of the yield traits(except 1 000-grain weight whose broad and narrow heritability were both over70%) were all below 50% at the two experimental bases, suggesting that the four traits were easily subjected to environment influence. Very significant positive correlation of general combining ability was found between yield per plant and other traits except 1 000-grain weight. The general combining ability variance showed a normal distribution among the recombinant inbred lines at two sites, right in line with inheritance of quantitative traits. So, the genes controlling rice general combining ability can be targeted by QTL mapping. 展开更多
关键词 Hybrid rice recombinant inbred lines Yield traits Combining ability
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Mapping of QTLs for Sheath Blight Resistance Using Recombinant Inbred Lines of Rice(Oryza sativa L.)
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作者 杨娟 王莉娟 +1 位作者 黄胜东 李余生 《Agricultural Science & Technology》 CAS 2015年第7期1374-1377,共4页
A recombinant inbred line (RIL) population composed of 157 lines derived from an inter-subspecific hybrid of Daguandao/IR28 by the single seed descent method was used as materials, and the quantitative trait loci (... A recombinant inbred line (RIL) population composed of 157 lines derived from an inter-subspecific hybrid of Daguandao/IR28 by the single seed descent method was used as materials, and the quantitative trait loci (QTLs) coffering the resistance to sheath blight in the 157 RILs and the parents were detected using the toothpick inoculation method. The disease indexes of rice sheath blight in the two parents and 157 RILs were scored and the QTLs responsible for rice sheath blight resistance were detected accordingly by QTL Cartographer software. The results showed that a total of 4 QTLs (qsbl, qsb2, qsb5-1, qsb5-2) conferring sheath blight resistance were detected on chromosomes 1, 2 and 5, and their variance explained ranged from 10.41% to 36.92%. The additive effect of qsb5-1 was negative, indicat- ing that the QTLs derived from donor parent IR 28 could enhance the resistance to sheath blight. However, the additive effects of qsbl, qsb2 and qsb5-2 were positive, indicating that the QTLs derived from donor parent Daguandao weakened the resis- tance to sheath blight. 展开更多
关键词 Sheath blight resistance recombinant inbred lines Quantitative trait locus
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甘蓝型油菜RIL群体苗期根系耐铝性鉴定及评价方法研究
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作者 李晨阳 史红松 +8 位作者 徐璐 邢明礼 武晓如 白岩松 牛梦缘 高俊奇 李加纳 周清元 崔翠 《西南大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第4期24-36,共13页
为减少铵肥的大量施用以及酸雨频降引发的耕地酸化,从而影响作物的生长,利用综合评价方法通过幼苗期根系表型筛选耐铝种质.以甘蓝型油菜重组自交系(RIL)亲本10D130和中双11为材料筛选苗期适宜的铝胁迫浓度,使用该浓度处理RIL内138个品种... 为减少铵肥的大量施用以及酸雨频降引发的耕地酸化,从而影响作物的生长,利用综合评价方法通过幼苗期根系表型筛选耐铝种质.以甘蓝型油菜重组自交系(RIL)亲本10D130和中双11为材料筛选苗期适宜的铝胁迫浓度,使用该浓度处理RIL内138个品种(系)幼苗,对其根长、根表面积、根平均直径、根体积、根交叉数、根尖数和根干质量7个根系指标的铝毒单项耐性系数进行变异系数分析、相关性分析、主成分分析、系统聚类分析和逐步回归分析,并采用隶属函数法综合评价不同油菜品种(系)根系的耐铝特性.结果表明:138份油菜品种(系)间各项根系指标差异有统计学意义(p<0.05);筛选出甘蓝型油菜幼苗期耐铝毒品种(系)27034,27036和27007等.根据灰色关联度及逐步回归分析结果,认为根据根长、根表面积、根体积、根交叉数等7个关键指标,可以初步判断甘蓝型油菜种质幼苗期的耐铝毒特性.通过这些方法综合评价油菜对铝的耐受性,可以避免单一指标的片面性和不稳定性,结果更为可靠. 展开更多
关键词 甘蓝型油菜 重组自交系 耐铝性 苗期 根系
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Genetic analysis and QTL mapping of stalk cell wall components and digestibility in maize recombinant inbred lines from B73 × By804 被引量:2
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作者 Qi Wang Kun Li +5 位作者 Xiaojiao Hu Huimin Shi Zhifang Liu Yujin Wu Hongwu Wang Changling Huang 《The Crop Journal》 SCIE CAS CSCD 2020年第1期132-139,共8页
The cell wall composition and structure of the maize stalk directly affects its digestibility and in turn its feed value.Previous studies of stem quality have focused mostly on common maize germplasm,and few studies h... The cell wall composition and structure of the maize stalk directly affects its digestibility and in turn its feed value.Previous studies of stem quality have focused mostly on common maize germplasm,and few studies have focused on high-oil cultivars with high grain and straw quality.Investigation of the genetic basis of cell wall composition and digestibility of maize stalk using high-oil maize is desirable for improving maize forage quality.In the present study,a high-oil inbred line(By804)was crossed as male parent with the maize inbred line B73 to construct a population of 188 recombinant inbred lines(RILs).The phenotypes of six cell-wall-related traits were recorded,and QTL analysis was performed with a genetic map constructed with SNP markers.All traits were significantly correlated with one another and showed high broad-sense heritability.Of 20 QTLs mapped,the QTL associated with each trait explained 10.0%–41.1%of phenotypic variation.Approximately half of the QTL each explained over 10%of the phenotypic variation.These results provide a theoretical basis for improving maize forage quality by marker-assisted selection. 展开更多
关键词 MAIZE QTL Cell wall DIGESTIBILITY recombinant inbred lines
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Identification of Stable Quantitative Trait Loci for Sheath Blight Resistance Using Recombinant Inbred Line 被引量:1
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作者 CHEN Yuan ZENG Yuxiang +3 位作者 JI Zhijuan LIANG Yan WEN Zhihua YANG Changdeng 《Rice science》 SCIE CSCD 2019年第5期331-338,共8页
To identify stable quantitative trait loci(QTLs)responsible for sheath blight resistance,a recombinant inbred line mapping population consisting of 219 lines was developed by crossing Lemont and Yangdao 4.Average dise... To identify stable quantitative trait loci(QTLs)responsible for sheath blight resistance,a recombinant inbred line mapping population consisting of 219 lines was developed by crossing Lemont and Yangdao 4.Average disease rating,average lesion length,maximum disease rating and maximum lesion length were assayed in six different environments.A total of 128 minor effect QTLs were detected by multiple interval mapping.These QTLs explained less than 11.2%of the phenotypic variations individually,and 106 QTLs were clustered in 20 QTL-rich regions/putative loci.Significant QTL×environment interactions were detected at three putative loci(qSBR11.1,qSBR11.2 and qSBR11.3),indicating that these three loci were not stable.The other 17 stable loci(qSBR1.1,qSBR1.2,qSBR2.1,qSBR2.3,qSBR3.1,qSBR3.2,qSBR3.5,qSBR3.6,qSBR5.1,qSBR7.1,qSBR8.1,qSBR9.1,qSBR9.2,qSBR9.3,qSBR12.1,qSBR12.2 and qSBR12.4)provided a foundation for marker-assisted selection in breeding.Analysis of allelic effect on the 20 putative loci identified 7 highly stable loci,including qSBR3.2,qSBR7.1,qSBR8.1,qSBR9.2,qSBR9.3,qSBR12.1 and qSBR12.2. 展开更多
关键词 rice SHEATH blight resistance quantitative TRAIT LOCUS recombinant inbred line
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Diversity, Structure, and Marker-Trait Association Analysis of the Maize Recombinant Inbred Line Population 被引量:1
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作者 CHEN Jing-tang HU Li-zong ZHU Li-ying GUO Jin-jie ZHAO Yong-feng HUANG Ya-qun 《Agricultural Sciences in China》 CAS CSCD 2011年第7期975-986,共12页
Association mapping has emerged as a new tool to elucidate complex quantitative trait loci in maize, but there are few reports about systematic association analysis for the specific SSR markers with agronomic traits o... Association mapping has emerged as a new tool to elucidate complex quantitative trait loci in maize, but there are few reports about systematic association analysis for the specific SSR markers with agronomic traits of interest in China. We investigated the morphological and genetic diversity and population structure for 76 maize recombinant inbred lines, and then association analysis were further performed between 48 simple sequence repeat loci and 17 morphological traits, consisting of nine ear-related traits and eight other traits. The 48 SSR markers were screened out and further classified into two groups including a group of loci in regions harboring reported quantitative trait loci that affect ear shape and a group of markers distributing on the whole genome randomly. The result indicated that the population of recombinant inbred lines was structured, showing five subpopulations. Our association results revealed that there were 82, 59, and 40 significant associations detected by K-test, logistic regression, and both analysis, respectively. When the 17 traits were considered separately, the significant associations between Q-SSRs and E-traits were raised to 27.8%, whereas the other groups of combinations ranged between 2.3 and 6.3%. As the proportion of significant associations is higher among the Q-SSR subset of markers and the subset of traits related to ear shape than those for all of the other combinations, we conclude that this approach is valid for establishing true positive marker-trait relationships. Our results also demonstrated that association mapping could complement and enhance previous QTL information for marker-assisted selection. 展开更多
关键词 MAIZE ear shape association mapping recombinant inbred lines
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Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant Inbred Rice Lines
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作者 Jirapong Jairin Phanchita Vejchasarn +9 位作者 Thanapa Somjai Kanuengnij Srivilai Kulchana Darwell Phikul Leelagud Rungnapa Kawichai Jate Kotcharerk Arissara Suthanthangjai Nattaya Popa Suphalaksana Lachanthuek Varapong Chamarerk 《American Journal of Plant Sciences》 2019年第5期760-779,共20页
Rice is the most significant global food security. Several biotic factors limit rice production, breeding biotic-resistant rice has, therefore, become an increasingly important goal. Two elite rice lines, IR71033-121-... Rice is the most significant global food security. Several biotic factors limit rice production, breeding biotic-resistant rice has, therefore, become an increasingly important goal. Two elite rice lines, IR71033-121-15 (IR71033) and IR57514-PMI-5-B-1-2 (IR57514), provide potential genes for biotic stress resistance traits. In this study, genotyping by sequencing (GBS) for single nucleotide polymorphism (SNP)-based linkage map construction was used to detect quantitative trait loci (QTLs) for blast (BL), bacterial blight (BB), whitebacked planthopper (WBPH), and brown planthopper (BPH) resistance. IR71033 was derived from Oryza minuta and carried BL, BB, WBPH, and BPH resistance QTLs. IR57514 is a well-adapted rainfed lowland line that carries BL and BB resistance QTLs. Two sets of recombinant inbred line (RIL) populations derived from crosses of KDML105 × IR71033 and KDML105 × IR57514 were used to dissect the genetic basis of disease and insect pest resistance. The RIL populations were evaluated for BL, BB, WBPH, and BPH resistance from 2016 to 2018 at four rice research centers in Thailand. From these, we identified a large number of SNPs through GBS and constructed high-resolution linkage maps. By combining phenotypic evaluation with the GBS data, a total of 24 QTLs on four chromosomes were detected that confered pest resistance and explained 7.3% - 61.4% of the phenotypic variance. These findings should facilitate identifying novel resistance genes and applying marker-assisted selection for resistance to the four major rice pests investigated here. These strategies will improve the resilience and reliability of rice varieties adapted to the low-yielding environment of rainfed lowland areas worldwide. 展开更多
关键词 recombinant inbred line Genotyping by Sequencing RESISTANCE Gene RAINFED Lowland RICE Single Nucleotide Polymorphism Quantitative Trait Locus
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Identifying Genomic Regions Conferring Morphological and Yield-related Traits in Soybean Based On A Four-way Recombinant Inbred Line Population
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作者 Ning Hai-long Yang Chang +2 位作者 Hu Bo Sun Xu Li Wen-xia 《Journal of Northeast Agricultural University(English Edition)》 CAS 2022年第3期1-10,共10页
Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed n... Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed number per plant(NS),100-seed weight(HSW)and seed weight per plant(SWPP).Identifying quantitative trait loci(QTLs)for morphological and yield-related traits is therefore important for breeding.In this study,a four-way recombinant inbred line population comprising 160 lines derived from the cross(Kenfeng14×Kenfeng15)×(Heinong48×Kenfeng19)was planted in five different environments and morphological and yield-related trait data were used to identify QTLs by the inclusive composite interval mapping method.Totally 38 QTLs for PH,40 QTLs for NN,26 QTLs for NP,10 QTLs for NS,26 QTLs for HSW and 49 QTLs for SWPP were detected in 125 genomic regions.Single QTLs explained 2.17%-14.60%,2.00%-10.04%,2.37%-9.77%,2.62%-8.61%,0.47%-6.51%and 0.14%-12.39%of the phenotypic variation for PH,NN,NP,NS,HSW and SWPP,respectively.Among these 125 genomic regions,120 were newly associated with morphological and yield-related traits.The results would facilitate the molecular breeding of morphological and yield-related traits in soybean. 展开更多
关键词 SOYBEAN morphological and yield-related traits QTL four-way recombinant inbred line population
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Genetic analysis and QTL mapping of growth period traits and plant height traits in soybean recombinant inbred lines from Dongnong 47 × PI 317334-B
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作者 Jing Li Jiafan Sun +2 位作者 Minmin Li Xue Zhao Lin Zhao 《Oil Crop Science》 CSCD 2021年第2期66-73,共8页
High and stable yield is the main goal of soybean genetic improvement.In this study,association analysis was used to detect the quantitative trait loci(QTL)for the plant height,and soybean growth period using 182 SSR ... High and stable yield is the main goal of soybean genetic improvement.In this study,association analysis was used to detect the quantitative trait loci(QTL)for the plant height,and soybean growth period using 182 SSR markers in the RIL population of 136 F_(4:8) lines,which developed from a cross between photoperiod-insensitive cultivar‘Dongnong 47’and photoperiod-sensitive variety PI317334–B.The results showed that 33 QTLs related to soybean growth period and plant height traits were detected by compound interval mapping,and were located on 12 linkage groups including N,C1,C2,J,D1a,B2,E,G,A2,O,L,I,with the contribution rate of 7.85–33.84%.These QTL loci and linkage markers related to soybean photoperiod sensitivity,would be helpful to identify key genes that control soybean photoperiod sensitivity,and provide an important basis for the breeding of new photoperiod-insensitive soybean varieties based on molecular design breeding. 展开更多
关键词 SOYBEAN Photoperiod response recombinant inbred lines SSR markers QTL genetic map
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基于F2和RIL群体鉴定棉花抗黄萎病主效QTL
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作者 赵云雷 王文菊 +9 位作者 陈伟 王海红 朱喜霞 杨继华 鲁宁宁 赵佩 桑晓慧 崔艳利 敦磊 王红梅 《棉花学报》 CSCD 北大核心 2023年第2期101-116,共16页
【目的】通过对棉花抗黄萎病性状进行数量性状位点(quantitative trait locus,QTL)定位,鉴定可以应用于育种实践的能稳定检测到的主效QTL,为棉花抗黄萎病遗传改良奠定分子基础。【方法】以抗黄萎病品种中植棉2号和感黄萎病品种冀棉11号... 【目的】通过对棉花抗黄萎病性状进行数量性状位点(quantitative trait locus,QTL)定位,鉴定可以应用于育种实践的能稳定检测到的主效QTL,为棉花抗黄萎病遗传改良奠定分子基础。【方法】以抗黄萎病品种中植棉2号和感黄萎病品种冀棉11号为亲本杂交的F2群体和重组自交系(recombinant inbred lines,RIL)群体作为作图群体,在对2个群体进行多环境黄萎病抗性鉴定和简单重复序列(simple sequence repeat,SSR)分子标记检测的基础上进行遗传连锁图谱构建,利用完备区间作图法进行QTL定位,并对获得的主效QTL置信区间进行候选基因挖掘。【结果】在F2:3家系和RIL群体中共检测到7个抗黄萎病QTL,能够在多个环境条件下重复检测到的QTL有4个,包括q VW-D05-1、qVW-D05-2、q VW-D05-4和qVW-D05-5。共线性分析表明上述4个QTL集中分布于D05染色体上2293776~3205058 bp和62407897~62582344 bp 2个区域。4个抗性QTL的聚合可以显著降低棉花黄萎病病情指数。qVW-D05-1在多个环境中的表型变异解释率均在10%以上,为抗黄萎病主效QTL。对qVW-D05-1置信区间内的基因进行了功能注释、黄萎病菌诱导后的表达模式分析和单核苷酸多态性(single nucleotide polymorphism,SNP)变异分析,初步推测Ghir_D05G038990、Ghir_D05G039060、Ghir_D05G039100、Ghir_D05G039110和Ghir_D05G039130为可能控制棉花黄萎病抗性的候选基因。【结论】在不同环境中共检测到7个抗黄萎病QTL,其中4个QTL被重复检测到,在主效QTL q VW-D05-1区间内筛选获得5个棉花抗黄萎病候选基因。这些稳定的抗黄萎病相关的QTL及其候选基因可应用于棉花抗黄萎病性状的分子标记辅助选择育种。 展开更多
关键词 棉花 黄萎病 QTL定位 重组自交系 基因挖掘
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野生大豆×栽培大豆RIL群体高密度遗传图谱构建及SNP偏分离分析 被引量:2
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作者 刘德泉 聂波涛 +5 位作者 邱红梅 陈亮 陈健 崔正果 姬文秀 王跃强 《大豆科学》 CAS CSCD 北大核心 2023年第6期641-652,共12页
通过研究野生大豆与栽培种大豆群体构建过程中产生的偏分离现象,发掘偏分离区间(Segregation Distortion Region, SDR)和候选基因,有助于探究偏分离在大豆中的产生机制。应用地方品种“一千粒”和野生品种“长岭野生豆”配置杂交组合,获... 通过研究野生大豆与栽培种大豆群体构建过程中产生的偏分离现象,发掘偏分离区间(Segregation Distortion Region, SDR)和候选基因,有助于探究偏分离在大豆中的产生机制。应用地方品种“一千粒”和野生品种“长岭野生豆”配置杂交组合,获得F6代重组自交系(Recombinant Inbred Lines, RIL)株系200株,利用SLAF-seq进行测序分析,构建高密度遗传图谱,获得该群体可靠的4 564个SNP标记。偏分离分析发现,648个标记发生偏分离(P<0.05),占总标记的14.20%。获得22个SDR,分布在9个不同的染色体上。在SDR区间内共发现8个重度偏分离热点区域(Extreme Segregation Distortion, ESDR),分布在5个不同的染色体上,其中3个ESDR偏向父本野生型,5个ESDR偏向母本栽培型。利用基因功能注释及全基因组重测序数据,结合ESDR区域,影响胚胎发育(Glyma.01G051400)及雌配子体发育(Glyma.16G072700)的基因分别被认为是ESDR1-1和ESDR16-1的候选基因。本研究结果为今后偏分离基因定位提供可靠依据,并为阐明偏分离现象奠定基础。 展开更多
关键词 栽培大豆 野生大豆 偏分离 SNP 重组自交系
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QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice(Oryza sativa L.) 被引量:7
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作者 GAO Fang-yuan ZENG Li-hua +6 位作者 QIU Ling LU Xian-jun REN Juan-sheng WU Xian-ting SU Xiang-wen GAO Yong-ming REN Guang-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第8期1693-1702,共10页
supported by a grant from the National High-Tech R&D Program of China (2014AA10A603, 2014AA10A604);a grant from the Youth Foundation in Sichuan, China (2011JTD0022);the special fund for China Agricultural Researc... supported by a grant from the National High-Tech R&D Program of China (2014AA10A603, 2014AA10A604);a grant from the Youth Foundation in Sichuan, China (2011JTD0022);the special fund for China Agricultural Research System (CARS-01-08);the Provincial Specialized Funds for Innovation Ability Promotion in Sichuan, China (2013GXJS005) 展开更多
关键词 QTL appearance quality 1000-grain weight recombination inbred lines(ril rice
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不同遗传背景玉米RIL家系种子的活力特征 被引量:1
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作者 赵博文 王彬 +3 位作者 纪坤 薛浩 赵霖熙 韩赞平 《贵州农业科学》 CAS 2023年第6期14-21,共8页
【目的】探明玉米重组自交系(RILs)家系群体种子的活力特征,为玉米优异种质资源创制和强优势杂交组合配置提供参考。【方法】利用豫82×沈137(群体1)和豫537A×沈137(群体2)重组自交系家系及其3个亲本的玉米种子为材料,对其籽... 【目的】探明玉米重组自交系(RILs)家系群体种子的活力特征,为玉米优异种质资源创制和强优势杂交组合配置提供参考。【方法】利用豫82×沈137(群体1)和豫537A×沈137(群体2)重组自交系家系及其3个亲本的玉米种子为材料,对其籽粒性状(百粒重、籽粒长、籽粒宽、含水量、电导率、蛋白质含量、可溶性糖含量、丙二醛含量)、发芽性状(发芽率、发芽势、发芽指数、活力指数)及幼苗性状(苗长、苗干重)进行描述性分析、相关性分析和主成分分析。【结果】群体1和群体2均以发芽势的变异系数最大,分别为56.38%和68.67%;籽粒宽、百粒重、苗长、苗干重、发芽率、发芽势、发芽指数、活力指数等8个性状的变异系数群体2大于群体1。在2个群体的籽粒性状与发芽性状关系中,除群体2的百粒重与发芽率和活力指数呈显著正相关外,其他籽粒性状与发芽性状均无显著正相关。主成分分析将群体1和群体2的14个性状分别转化为5个和4个主成分,累计贡献率分别为78.778%和76.414%。【结论】2个玉米RILs群体的百粒重、苗干重和发芽率呈双向超亲分离,发芽势变异系数最大。活力指数、发芽指数、发芽势和发芽率显著相关;群体1的籽粒性状与发芽性状无显著相关,群体2的百粒重与发芽率和活力指数呈正相关。基于主成分分析对2个玉米RILs群体的籽粒性状和发芽性状进行综合评价,群体2的得分明显高于群体1,群体2中得分高的株系可用于育种实践。 展开更多
关键词 玉米 重组自交系 发芽性状 种子活力 相关性
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Mapping and Comparative Analysis of QTL for Rice Plant Height Based on Different Sample Sizes within a Single Line in RIL Population
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作者 Yong-shu LIANG Zhi-qiang GAO +5 位作者 Xi-hong SHEN Xiao-deng ZHAN Ying-xin ZHANG Wei-ming WU Li-yong CAO Shi-hua CHENG 《Rice science》 SCIE 2011年第4期265-272,共8页
To clarify the most appropriate sample size for obtaining phenotypic data for a single line,we investigated the main-effect QTL(M-QTL) of a quantitative trait plant height(ph) in a recombinant inbred line(RIL) populat... To clarify the most appropriate sample size for obtaining phenotypic data for a single line,we investigated the main-effect QTL(M-QTL) of a quantitative trait plant height(ph) in a recombinant inbred line(RIL) population of rice(derived from the cross between Xieqingzao B and Zhonghui 9308) using five individual plants in 2006 and 2009.Twenty-six ph phenotypic datasets from the completely random combinations of 2,3,4,and 5 plants in a single line,and five ph phenotypic datasets from five individual plants were used to detect the QTLs.Fifteen M-QTLs were detected by 1 to 31 datasets.Of these,qph7a was detected repeatedly by all the 31 ph datasets in 2006 and explained 11.67% to 23.93% of phenotypic variation;qph3 was detected repeatedly by all the 31 datasets and explained 5.21% to 7.93% and 11.51% to 24.46% of phenotypic variance in 2006 and 2009,respectively.The results indicate that the M-QTL for a quantitative trait could be detected repeatedly by the phenotypic values from 5 individual plants and 26 sets of completely random combinations of phenotypic data within a single line in an RIL population under different environments.The sample size for a single line of the RIL population did not affect the efficiency for identification of stably expressed M-QTLs. 展开更多
关键词 RICE plant height QTL mapping recombination inbred line simple sequence repeat sample size
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玉米RIL群体穗部性状与单株产量的通径分析与灰色关联度分析
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作者 马兴业 李敏 +3 位作者 赵斌 王苗苗 陈士林 吴向远 《河南科技学院学报(自然科学版)》 2023年第6期1-6,共6页
为探究玉米农艺性状之间的相互作用及其对产量的影响,研究以PH6WC和郑58构建的重组自交系群体作为试验材料,运用相关和通径与灰色关联度分析方法对玉米的单株产量和6个穗部性状进行分析.描述统计分析表明,该RIL群体中秃尖长、单株产量... 为探究玉米农艺性状之间的相互作用及其对产量的影响,研究以PH6WC和郑58构建的重组自交系群体作为试验材料,运用相关和通径与灰色关联度分析方法对玉米的单株产量和6个穗部性状进行分析.描述统计分析表明,该RIL群体中秃尖长、单株产量、行粒数、穗长、穗行数的变异系数超过10%,说明这些性状具有较高的改良性;相关性分析表明,单株产量和穗部性状之间的相关程度为行粒数(第1位)、穗粗(第2位)、穗长(第3位)、穗行数(第4位)、轴粗(第5位)、秃尖长(第6位);通径分析表明,穗粗对单株产量的直接影响最大,直接通径系数为0.725,轴粗通过穗粗对单株产量的间接影响最大,间接通径系数为0.559.利用灰色关联度分析方法和通径分析方法所得结果一致,相关数据可为玉米新品种选育提供依据. 展开更多
关键词 玉米 重组自交系 穗部性状 通径分析 灰色关联度分析
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烟草生物碱性状的QTL定位 被引量:2
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作者 刘颖超 方敦煌 +2 位作者 徐海明 童治军 肖炳光 《作物学报》 CAS CSCD 北大核心 2024年第1期42-54,共13页
生物碱是烟草的重要化学成分。为明确烟草生物碱的遗传结构,发掘控制相关性状的主效位点,以烟草品种Y3、K326为亲本,构建大小为271的重组自交系群体。分别于2018、2019和2020年在云南省昆明市石林、玉溪市研和种植群体材料,检测总植物碱... 生物碱是烟草的重要化学成分。为明确烟草生物碱的遗传结构,发掘控制相关性状的主效位点,以烟草品种Y3、K326为亲本,构建大小为271的重组自交系群体。分别于2018、2019和2020年在云南省昆明市石林、玉溪市研和种植群体材料,检测总植物碱(TPA)、烟碱(NIC)、降烟碱(NOR)、假木贼碱(ANAB)和新烟草碱(ANAT)5种生物碱表型。对群体进行基因组测序,构建包含46,129个标记的遗传连锁图谱。利用基于混合线性模型的QTL定位方法及软件QTLNetwork2.0,进行QTL定位分析。共定位15个具有显著加性效应的QTL,加性效应对表型贡献率为0.58%~11.57%。其中4个主效QTL即控制总植物碱的qTPA14、烟碱的qNIC14、假木贼碱的qANAB14和新烟草碱的qANAT14,均可以解释相应性状10%以上的表型变异,且均位于14号连锁群上。6个QTL具有显著的加性与环境互作效应,对表型贡献率为0.80%~1.81%。5对QTL具有显著加性-加性上位性效应,对表型的贡献率为0.15%~2.31%。2对QTL具有显著的上位性与环境互作效应,对表型的贡献率为0.81%~1.16%。研究结果为进一步分离候选基因、解析遗传机理和促进烟草生物碱性状分子改良奠定了基础。 展开更多
关键词 烟草 重组自交系 生物碱 复杂性状 基因与环境互作
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济麦44/济麦229重组自交系群体籽粒蛋白质含量QTL分析 被引量:1
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作者 单宝雪 刘秀坤 +9 位作者 肖延军 展晓孟 黄金鑫 刘百川 张玉梅 李豪圣 刘建军 高欣 曹新有 赵振东 《山东农业科学》 北大核心 2024年第1期1-9,共9页
小麦籽粒蛋白质含量与面团流变学特性及加工特性的关系密不可分。本研究在2020—2021年济南试验基地(E1)及2021—2022年济南试验基地(E2)和济阳试验基地(E3)三个环境下,以“济麦44×济麦229”构建的包含285个家系的重组自交系群体(F... 小麦籽粒蛋白质含量与面团流变学特性及加工特性的关系密不可分。本研究在2020—2021年济南试验基地(E1)及2021—2022年济南试验基地(E2)和济阳试验基地(E3)三个环境下,以“济麦44×济麦229”构建的包含285个家系的重组自交系群体(F2∶6RILs)为材料,利用小麦55K SNP芯片构建高密度遗传连锁图谱,对籽粒蛋白质含量进行QTL分析。结果共筛选到2344个SNP标记用于构建遗传连锁图谱,图谱总长度3349.95 cM,平均标记密度为1.43 cM/标记。通过对籽粒蛋白质含量的QTL分析,共检测到18个籽粒蛋白质含量相关QTL,分布在1A、1B、2B、3D、4B、4D、5A、5B、5D、7A、7B共11条染色体上。Qpc.saas.4B-1在E1、E2和E3三个环境和BLUE(最佳线性无偏估计)值中均被稳定检测到,可以解释3.26%~23.79%的表型变异。Qpc.saas.4D和Qpc.saas.5A在两个环境和BLUE值中被检测到,分别解释2.42%~11.18%和2.48%~5.47%的表型变异,且Qpc.saas.4D与小麦矮秆基因Rht-D1b物理位置重合。本研究中检测到的QTL新位点Qpc.saas.4B-1、Qpc.saas.4D和Qpc.saas.5A是控制籽粒蛋白质含量的主效基因,具有高表型变异解释率。本研究结果可为小麦品质育种提供分子标记及理论参考。 展开更多
关键词 小麦 籽粒蛋白质含量 QTL分析 重组自交系 55K SNP芯片
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基于不同遗传群体的大豆分枝数QTL定位分析
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作者 朱晨博 张东梅 +7 位作者 孙明明 刘淼 袁明 贾晓轲 赵艳强 张艳婷 杨明亮 陈庆山 《中国油料作物学报》 CAS CSCD 北大核心 2024年第5期1017-1028,共12页
分枝数是大豆重要的农艺性状,与大豆株型结构、产量潜力和适应性密切相关。为挖掘大豆分枝数稳定遗传位点,本研究以多分枝大豆Charleston和主茎型大豆东农594为亲本构建的148份重组自交系群体(RILs)和以主茎型大豆绥农14和多分枝野生大... 分枝数是大豆重要的农艺性状,与大豆株型结构、产量潜力和适应性密切相关。为挖掘大豆分枝数稳定遗传位点,本研究以多分枝大豆Charleston和主茎型大豆东农594为亲本构建的148份重组自交系群体(RILs)和以主茎型大豆绥农14和多分枝野生大豆ZYD00006为亲本构建的213份染色体片段代换系(CSSLs)2个遗传群体为试验材料,采用ICIMapping软件中的完备区间作图法(inclusive composite interval mapping,ICIM)和Win-QTL-Cart 2.5软件中的复合区间作图法(composite interval mapping,CIM),对2018-2021年两个遗传群体的分枝数表型数据进行QTL分析,对QTL置信区间内的候选基因进行挖掘。共检测到17个与大豆分枝数性状相关的QTL位点,其中qBNA2_1和qBNK_1在不同年份和不同群体中稳定出现,分布在第8和9号染色体。根据基因注释等信息对两个QTL区间内的候选基因进行筛选,并通过qRT-PCR预测Glyma.08G053700、Glyma.08G068200、Glyma.08G082400和Glyma.09G167100为调控分枝数的候选基因。本研究结果有助于探明大豆分枝数形成的遗传机制,为大豆理想株型研究提供候选基因与材料支撑。 展开更多
关键词 大豆 重组自交系(ril) 染色体片段代换系(CSSL) 分枝数 QTL 候选基因
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利用RIL群体分析小麦淀粉粘度性状与馒头品质的关系 被引量:10
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作者 范玉顶 李斯深 +1 位作者 孙海艳 李瑞军 《中国粮油学报》 EI CAS CSCD 北大核心 2005年第1期6-8,26,共4页
利用重组自交系群体对淀粉粘度性状与馒头品质间的关系进行了全面分析。相关分析表明,峰值粘 度、低谷粘度、最终粘度、反弹值、峰值时间均与馒头外观、结构、弹韧性、粘性、总分等5项指标呈显著正相关;回归分 析结果表明,粘度性状与馒... 利用重组自交系群体对淀粉粘度性状与馒头品质间的关系进行了全面分析。相关分析表明,峰值粘 度、低谷粘度、最终粘度、反弹值、峰值时间均与馒头外观、结构、弹韧性、粘性、总分等5项指标呈显著正相关;回归分 析结果表明,粘度性状与馒头品质间存在较强的线性关系,建立了馒头体积、比容、结构、弹韧性、粘性和总分与淀粉 粘度性状的回归方程;通过通径分析明确了各粘度性状对馒头品质的直接效应和间接效应。 展开更多
关键词 性状 馒头品质 ril群体 淀粉粘度 利用 重组自交系群体 通径分析 小麦淀粉 粘性 比容
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