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Identification of Panton-Valentine leukocidin virulence gene in methicillin-resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran
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作者 Atefeh Ranjkesh Zahra Salari +14 位作者 Mehrab Nejati Emad Behboudi Ali Ramezani Milad Zandi Mahmood Marzban Hadis Kiani Mina Bavi Mehdi Rabiei Roodsari Yousef Esvand Zibaee Samaneh Abbasi Maryam Mohammadi Elyasi Mehdi Parsanahad Shahram Jalilian SiminMohammadi Gorji Mina Owrang 《Journal of Acute Disease》 2020年第3期121-125,共5页
Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A ... Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017.All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies.Then the presence of the Panton-Valentine leukocidin gene was tested by PCR.Results:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the Panton-Valentine leukocidin gene,and only 20%of the samples had Panton-Valentine leukocidin gene.Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene.Conclusions:In recent years,increased resistance has been a serious threat.The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations. 展开更多
关键词 METHICILLIN Resistance panton-valentine leukocidin PCR STAPHYLOCOCCUS AUREUS
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Screening for Panton-Valentine Leukocidin Toxin Genes in Multi-Drug Resistant Methicillin-Resistant <i>Staphylococcus aureus</i>Nasal Isolates from Healthy School Children in Mariental, Namibia
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作者 Sunette Walter Ronnie Böck 《Journal of Biosciences and Medicines》 2020年第12期13-19,共7页
Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of <em>Staphylococcus aureus</em>. In school settings where children are in close contact with each other, <em&... Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of <em>Staphylococcus aureus</em>. In school settings where children are in close contact with each other, <em>S. aureus</em> strains, including those that may produce PVL, can be transmitted and spread in the community. Twenty-two multi-drug resistant MRSA nasal isolates from children enrolled in five schools in the town of Mariental and the multi-drug resistant American Type Culture Collection MRSA reference strain <em>S. aureus</em> ATCC 33591 (PVL-negative control) were used for molecular assays. Plasmid deoxyribonucleic acid (DNA) of isolates was amplified by polymerase chain reaction (PCR) and amplified PCR products were electrophoresed on a 2.5% (w/v) agarose gel containing 12 μl 0.5 μg/ml ethidium bromide and 1× TAE (Tris-acetate-EDTA) buffer at 90 volts for 50 minutes. The developed gel was viewed for the PVL-associated <em>lukS</em> and <em>lukF</em> genes that amplified at 151 bp and 406 bp, respectively. Our results indicated that seven nasal isolates had PVL toxin gene(s). From the seven isolates, three were tested positive for both <em>lukS</em> and<em> lukF</em>genes, one tested positive for only<em> lukS</em>, and three tested positive for only <em>lukF</em>. Two of the isolates harbouring both<em> lukS</em> and <em>lukF</em> genes shared the same antibiotic resistance pattern and one of them could also produce enterotoxin A. One of the isolates with only <em>lukF </em>gene could produce enterotoxins B and C. These toxin-producing isolates can be expected to be more virulent than non-producers. Children should be educated on the importance of regular handwashing with soap and water to prevent the spread of potentially virulent staphylococci amongst them and the wider community. This work warrants a larger study to be carried out to investigate PVL toxin and its associated infections in <em>Staphylococcus</em> from school children in Namibia. 展开更多
关键词 Methicillin-Resistant Staphylococcus aureus panton-valentine leukocidin Children
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Identification of a T-cell epitope in the <i>Staphylococcus aureus</i>Panton-Valentine LukS-PV component
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作者 Eric L. Brown Keri Csencsits Smith Maria Gabriela Bowden 《Open Journal of Immunology》 2012年第3期111-115,共5页
We previously observed the elicitation of a significant delayed-type hypersensitivity response to the Panton-Valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al., Clinical Micro... We previously observed the elicitation of a significant delayed-type hypersensitivity response to the Panton-Valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al., Clinical Microbiology and Infection, 2009, 156: 156-164). A LukS-PV-specific cell line (LST) was screened for proliferative responses against a panel of 25 amino acid-long peptides spanning the length of LukS-PV (amino acids 29-312). This analysis demonstrated that stimulation of LST with LukS-PV resulted in significant proliferative responses and adoptive transfer of LST into na?ve mice conferred a LukS-PV-specific DTH response following challenge. Challenge of mice adoptively transferred with LST with peptides 7 (149-173), 8 (169-193) and 14 (289-312) also elicited a measurable DTH response suggesting that these peptides contained T cell epitopes. 展开更多
关键词 STAPHYLOCOCCUS Cell Mediated Immunity panton-valentine leukocidin T Cells
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rPVL对Balb/C小鼠肺组织损伤及细胞因子的影响 被引量:1
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作者 高朋 马筱玲 +3 位作者 常文娇 周馨 张翠萍 周文静 《临床肺科杂志》 2011年第6期826-828,共3页
目的研究rPVL对Balb/C小鼠肺组织损伤及肺组织匀浆中细胞因子的影响。方法 20只成年Balb/C小鼠分两组,对照组、rPVL组。对照组注射生理盐水,rPVL组注射rPVL,两组分别于注射后3 h、9 h处死小鼠,每个时间段5只,ELISA方法测肺组织匀浆中TNF... 目的研究rPVL对Balb/C小鼠肺组织损伤及肺组织匀浆中细胞因子的影响。方法 20只成年Balb/C小鼠分两组,对照组、rPVL组。对照组注射生理盐水,rPVL组注射rPVL,两组分别于注射后3 h、9 h处死小鼠,每个时间段5只,ELISA方法测肺组织匀浆中TNF-α、IL-8水平,RT-PCR方法测肺组织匀浆TNF-α、IL-8mRNA的表达。结果 rPVL刺激小鼠后3 h、9 h肺组织匀浆TNF-α、IL-8蛋白含量、mRNA表达量均高于正常对照组,差异有统计学意义(P<0.05),rPVL组TNF-α、IL-8蛋白含量、mRNA表达量随时间延长而升高,差异有统计学意义(P<0.05)。结论 rPVL能引起小鼠肺组织严重损伤,且肺组织匀浆中TNF-α、IL-8大量释放,提示细胞因子在rPVL对小鼠肺组织损伤过程中起重要作用。 展开更多
关键词 重组杀白细胞毒素 肿瘤坏死因子-Α 白细胞介素-8
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金黄色葡萄球菌杀白细胞素基因敲除菌株的构建及生长特性分析 被引量:1
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作者 刘文宇 祝瑶 +6 位作者 王长珍 杨亲 栾天 王凌利 刘思国 周学章 张万江 《中国预防兽医学报》 CAS CSCD 北大核心 2020年第11期1104-1108,共5页
为构建金黄色葡萄球菌杀白细胞素(PVL)基因敲除的菌株,本研究以pBT2温敏型质粒为载体,构建金黄色葡萄球菌PVL基因敲除质粒pBT2-ΔPVL,并经酶切鉴定。将其转化至金黄色葡萄球菌RN4220中经其修饰后电转化至金黄色葡萄球菌ATCC 49775中,在3... 为构建金黄色葡萄球菌杀白细胞素(PVL)基因敲除的菌株,本研究以pBT2温敏型质粒为载体,构建金黄色葡萄球菌PVL基因敲除质粒pBT2-ΔPVL,并经酶切鉴定。将其转化至金黄色葡萄球菌RN4220中经其修饰后电转化至金黄色葡萄球菌ATCC 49775中,在30℃和42℃多次交替传代并经红霉素抗性筛选,构建PVL基因敲除菌株,经PCR和测序鉴定后命名为ΔPVL。以pLI50穿梭质粒为载体,构建金黄色葡萄球菌PVL基因回补质粒pLI50-PVL,按照同样方法将其转化至敲除菌株ΔPVL中,构建PVL基因回补菌株CΔPVL。将ΔPVL在TSB培养基中连续传代培养后,经PCR检测敲除菌株体外的遗传稳定性;分别绘制敲除菌株ΔPVL和回补菌株CΔPVL的生长曲线,检测其体外生长特性。结果显示,正确构建了两种质粒pBT2-ΔPVL、pLI50-PVL和两种菌株ΔPVL、CΔPVL。敲除菌株ΔPVL连续传代未发生回复突变,具有良好的遗传稳定性。生长特性结果显示敲除菌株生长速率显著低于野生菌株,表明PVL基因在金黄色葡萄球菌的生长调节中发挥着一定的作用。本研究为进一步研究金黄色葡萄球菌PVL的功能及致病机制奠定了基础。 展开更多
关键词 杀白细胞素 同源重组 遗传稳定性
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Molecular Diversity of <i>Staphylococcus aureus</i>from the Nares of Hospital Personnel, HIV-Positive and Diabetes Mellitus Patients in Yaounde Cameroon
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作者 Agnes Eyoh Marthie Ehlers +11 位作者 Emilia Lyonga Mbamyah John Antiabong Charles Fokunang Marleen Kock Marie Claire Okomo Assoumou Michel Toukam Hortense Gonsu Kamga George Ikomey Martha Mesembe Mandi Henshaw Christiana Haddison Sinata Koulla-Shiro 《Advances in Microbiology》 2021年第12期740-756,共17页
Nasal carriage of <i>Staphylococcus aureus</i> has been identified as a risk factor for the development of staphylococcal infections caused by endogenous colonizing strains. Information on the genotypic di... Nasal carriage of <i>Staphylococcus aureus</i> has been identified as a risk factor for the development of staphylococcal infections caused by endogenous colonizing strains. Information on the genotypic diversity of <i>Staphylococcus aureus</i> is relevant for managing epidemiological and clinical challenges resulting from the evolutionary differences of this bacterium. The objective of this study was to determine and compare the molecular diversity of <i>Staphylococcus aureus</i> isolates from three high-risk populations in Yaounde, Cameroon. Molecular analysis confirmed that 95% of 100 tested isolates were <i>S. aureus</i>. The <i>mec</i>A and Panton Valentine-Leukocidin (PVL) genes (<i>lukS/F-PV</i>) were detected in 37% (35/95) and 43% (41/95) of isolates respectively and 18% (17/95) of the isolates harboured both the <i>mec</i>A and <i>lukS/F-PV</i> genes. A mixed distribution of both methicillin sensitive <i>S. aureus</i> (MSSA)/PVL and methicillin resistant <i>S. aureus</i> (MRSA)/PVL strains were detected within the study population. Community associated MRSA accounted for 94% (33/35) of the isolates, further classified into allotypes SCC<i>mec</i> type IV 54% (19/35) and SCC<i>mec</i> type V 40% (14/35), while two isolates were hospital associated SCC<i>mec</i> type II strains. A majority of the isolates harboured a single aggressive gene regulator allele <i>agr</i> type I. Pulsed Field Gel Electrophoresis (PFGE) generated 18 pulsotypes that grouped isolates irrespective of the study population. Multilocus Sequence Typing (MLST) of 12 selected isolates was assigned to six pandemic clonal complexes (CC): CC5 (ST5), CC8 [ST8, (n = 3)], CC15 (ST 15), CC25 (ST 25), CC72 [ST72 (n = 2)] and CC121 [ST 121 (n = 2)] and three atypical sequence types ST 508, ST 699 (CC45) and ST 1289 (CC 88). The study population represents an important reservoir for MRSA, MRSA-PVL and MSSA-PVL which could serve as focal point for further dissemination bringing about significant clinical and epidemiological implications. The predominance of SCC<i>mec</i> IV and <i>agr</i> types in this setting warrants further investigation. Isolates were genetically diverse with MLST indicating that pandemic ST8 was predominant. Detection of atypical STs has provided an insight into the necessity for constant monitoring. 展开更多
关键词 Nasal Carriage Methicillin Resistant S. aureus Methicillin Sensitive S. aureus panton-valentine leukocidin Multilocus Sequence Typing
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