Through analysis on existing problems and rationality of East Street reconstruction project,its social benefit had been analyzed sysmatically and comprehensively from the aspects of social environment,natural environm...Through analysis on existing problems and rationality of East Street reconstruction project,its social benefit had been analyzed sysmatically and comprehensively from the aspects of social environment,natural environment and traffic safety environment.Furthermore,evaluation indexes of East Street reconstruction project had been established and weighting of each index had been identified by analytic hierarchy process(AHP).Fuzzy Synthetic Evaluation Model had been founded through fuzzy synthetic evaluation method.Finally,scientific judgment was conducted on social benefit of East Street reconstruction project by applying established evaluation system.It demonstrated that East Street reconstruction project could bring great social benefit,so it should support this project.展开更多
Fertile topsoil was added onto the surface of barren slope land in Three Gorges Reservoir region of China in an anthropogenic process known as the foreign soil reconstruction project. The main goal of this paper was t...Fertile topsoil was added onto the surface of barren slope land in Three Gorges Reservoir region of China in an anthropogenic process known as the foreign soil reconstruction project. The main goal of this paper was to reveal the influence of anthropogenic activities on pedogenic processes and soil classifications. Chemical weathering indices and comparative analysis were applied to discuss changes in geochemical compositions and weathering features of purplish soils(Cambisols) before and after the project. Results showed that:(1) The foreign soil reconstruction project slightly altered the major element composition of topsoil and improved the soil structure. Although the distributions of major elements in the original topsoil, original subsoil, foreign topsoil and newly constructed topsoil were all similar to that in upper continental crust, newly constructed topsoil was the most similar soil.(2) The chemical index of alteration was more sensitive than the weathering index of Parker at indicating chemical weathering status of purplish soil. The chemical weathering status of newly constructed topsoil was higher than that of the original topsoil and lower than that of foreign topsoil.(3) Anthropogenic activities may provide a promising new thought for the anthropogenic soil classification system. The scope and subgroups of Anthrosols should be extended and strengthened. Or there may be a need to combine Anthrosols and Technosols orders to create a new soil order. The results may be used for optimizing soil mellowing engineering activities and enriching the soil classification system.展开更多
Aba Prefecture Company Name:Sichuan Xintong New Materials Co.Ltd. Address:Shuimo,Wenchuan Nature of Business:Private Project Name:Processing and Sale of Tourism Products Details:Producing and processing of Natural cry...Aba Prefecture Company Name:Sichuan Xintong New Materials Co.Ltd. Address:Shuimo,Wenchuan Nature of Business:Private Project Name:Processing and Sale of Tourism Products Details:Producing and processing of Natural crystal products,man-made gem and other展开更多
With the development of the compressive sensing theory, the image reconstruction from the projections viewed in limited angles is one of the hot problems in the research of computed tomography technology. This paper d...With the development of the compressive sensing theory, the image reconstruction from the projections viewed in limited angles is one of the hot problems in the research of computed tomography technology. This paper develops an iterative algorithm for image reconstruction, which can fit the most cases. This method gives an image reconstruction flow with the difference image vector, which is based on the concept that the difference image vector between the reconstructed and the reference image is sparse enough. Then the l1-norm minimization method is used to reconstruct the difference vector to recover the image for flat subjects in limited angles. The algorithm has been tested with a thin planar phantom and a real object in limited-view projection data. Moreover, all the studies showed the satisfactory results in accuracy at a rather high reconstruction speed.展开更多
Criterion of differential settlement in subgrade is useful for keeping road in a good serving condition. The theory of traditional materials strength and their technologic economy are applied to analyze the additional...Criterion of differential settlement in subgrade is useful for keeping road in a good serving condition. The theory of traditional materials strength and their technologic economy are applied to analyze the additional bend stress of the pavement structure, the equivalent beam of elastic foundation and influence on the pavement structure of an uneven settlement. The allowable settling ratio of pavement index ( △i ) is got and is used to appraise the uneven settlement standard value in subgrade. The allowable settling ratio of pavement index is applied in an certain widening expressway project and △i ≤0.36 % is attained by the analyzing, calculating and adding a certain safe coefficient. This value was put into practice and is effective during the past two years.展开更多
An improved algebraic reconstruction technique(ART) combined with tunable diode laser absorption spectroscopy(TDLAS) is presented in this paper for determining two-dimensional(2D) distribution of H2O concentrati...An improved algebraic reconstruction technique(ART) combined with tunable diode laser absorption spectroscopy(TDLAS) is presented in this paper for determining two-dimensional(2D) distribution of H2O concentration and temperature in a simulated combustion flame.This work aims to simulate the reconstruction of spectroscopic measurements by a multi-view parallel-beam scanning geometry and analyze the effects of projection rays on reconstruction accuracy.It finally proves that reconstruction quality dramatically increases with the number of projection rays increasing until more than 180 for 20 × 20 grid,and after that point,the number of projection rays has little influence on reconstruction accuracy.It is clear that the temperature reconstruction results are more accurate than the water vapor concentration obtained by the traditional concentration calculation method.In the present study an innovative way to reduce the error of concentration reconstruction and improve the reconstruction quality greatly is also proposed,and the capability of this new method is evaluated by using appropriate assessment parameters.By using this new approach,not only the concentration reconstruction accuracy is greatly improved,but also a suitable parallel-beam arrangement is put forward for high reconstruction accuracy and simplicity of experimental validation.Finally,a bimodal structure of the combustion region is assumed to demonstrate the robustness and universality of the proposed method.Numerical investigation indicates that the proposed TDLAS tomographic algorithm is capable of detecting accurate temperature and concentration profiles.This feasible formula for reconstruction research is expected to resolve several key issues in practical combustion devices.展开更多
A fast 3D reconstruction method based on structured light to measure various parameters of the raceway groove is presented. Digital parallel grating stripes distributed with sine density are projected onto the raceway...A fast 3D reconstruction method based on structured light to measure various parameters of the raceway groove is presented. Digital parallel grating stripes distributed with sine density are projected onto the raceway groove by a DLP projector, and distorting of stripes is happened on the raceway. Simultaneously, aided by three-step phase-shifting approach, three images covered by different stripes are obtained by a high-resolution CCD camera at the same location, thus a more accuracy local topography can be obtained. And then the bearing is rotated on a high precision computer controlled rotational stage. Three images are also obtained as the former step at next planned location triggered by the motor. After one cycle, all images information is combined through the mosaics. As a result, the 3D information of raceway groove can be gained. Not only geometric properties but also surface flaws can be extracted by software. A preliminary hardware system has been built, with which some geometric parameters have been extracted from reconstructed local topography.展开更多
CBCT scanners have been widely used in angiography,radiotherapy guidance,mammography and oral maxillofacial imaging.To cut detector size,reduce manufacturing costs and radiation dose while keeping a reasonable FOV,the...CBCT scanners have been widely used in angiography,radiotherapy guidance,mammography and oral maxillofacial imaging.To cut detector size,reduce manufacturing costs and radiation dose while keeping a reasonable FOV,the flat panel detector can be placed off-center horizontally.This scanning configuration extends the FOV effectively.However,each projection is transversely truncated,bringing errors and artifacts in reconstruction.In this paper,a simple but practical method is proposed for this scanning geometry based on truncation compensation and the modified FDK algorithm.Numerical simulations with jaw phantom were conducted to evaluate the accuracy and practicability of the proposed method.A novel CBCT system for maxillofacial imaging is used for clinical test,which is equipped with an off-center small size flat panel detector.Results show that reconstruction accuracy is acceptable for clinical use,and the image quality appears sufficient for specific diagnostic requirements.It provides a novel solution for clinical CBCT system,in order to reduce radiation dose and manufacturing cost.展开更多
Data collected in two-dimensional projections give planar images of object at each projection angle. To obtain information along the depth of the object, tomographic images are reconstructed using these projections. T...Data collected in two-dimensional projections give planar images of object at each projection angle. To obtain information along the depth of the object, tomographic images are reconstructed using these projections. There are basically two approaches to solve the problem of reconstruction: analytical and iterative, each one presenting its own advantages and limitations. This paper provides a detailed introduction and comparison to four analytical image reconstruction methods including Fourier transformation, simple back-projection, back-projection filtering and filtered back-projection.展开更多
To accelerate the scan speed and improve the image quality, a new type of CT configuration, "double-source double-multislice spiral CT" (DSDMS-CT), which is based on two sets of single-source multislice spir...To accelerate the scan speed and improve the image quality, a new type of CT configuration, "double-source double-multislice spiral CT" (DSDMS-CT), which is based on two sets of single-source multislice spiral CT was proposed with a special reconstruction algorithm. Simulation results using the fan-beam filtered backprojection algorithm with a special interpolation method were presented for both single-source multislice spiral CT and DSDMS-CT. The results of new CT model show that it scans faster than the traditional spiral CT and has a better slice sensitivity profile (SSP) with larger pitch value.展开更多
In the process of reconstruction and extension of highways,the most important thing is to ensure that the overall level of the widening of interchanges is comprehensively improved to ensure safe traffic.However,due to...In the process of reconstruction and extension of highways,the most important thing is to ensure that the overall level of the widening of interchanges is comprehensively improved to ensure safe traffic.However,due to the heavy and difficult tasks in the widening of interchanges,higher requirements are put forward for the construction quality of the project.In the actual construction of the project,it is necessary to actively analyze and explain the specific construction of the interchange widening project to ensure that the improvement level of the interchange widening in highway reconstruction and extension project is effectively upgraded.This paper gives a detailed introduction to the main construction technology for interchange widening project,clarifies the technical points of safety and guaranteed access,and proposes corresponding safeguard measures to ensure the comprehensive upgrade of the construction quality and standard of interchange widening in highway reconstruction and extension.展开更多
A fast converging sparse reconstruction algorithm in ghost imaging is presented. It utilizes total variation regularization and its formulation is based on the Karush-Kuhn-Tucker (KKT) theorem in the theory of convex ...A fast converging sparse reconstruction algorithm in ghost imaging is presented. It utilizes total variation regularization and its formulation is based on the Karush-Kuhn-Tucker (KKT) theorem in the theory of convex optimization. Tests using experimental data show that, compared with the algorithm of Gradient Projection for Sparse Reconstruction (GPSR), the proposed algorithm yields better results with less computation work.展开更多
Radial imaging techniques, such as projection-reconstruction (PR), are used in magnetic resonance imaging (MRI) for dynamic imaging, angiography, and short-T2 imaging. They are less sensitive to flow and motion ar...Radial imaging techniques, such as projection-reconstruction (PR), are used in magnetic resonance imaging (MRI) for dynamic imaging, angiography, and short-T2 imaging. They are less sensitive to flow and motion artifacts, and support fast imaging with short echo times. However, aliasing and streaking artifacts are two main sources which degrade radial imaging quality. For a given fixed number of k-space projections, data distributions along radial and angular directions will influence the level of aliasing and streaking artifacts. Conventional radial k-space sampling trajectory introduces an aliasing artifact at the first principal ring of point spread function (PSF). In this paper, a shaking projection (SP) k-space sampling trajectory was proposed to reduce aliasing artifacts in MR images. SP sampling trajectory shifts the projection alternately along the k-space center, which separates k-space data in the azimuthal direction. Simulations based on conventional and SP sampling trajectories were compared with the same number projections. A significant reduction of aliasing artifacts was observed using the SP sampling trajectory. These two trajectories were also compared with different sampling frequencies. ASP trajectory has the same aliasing character when using half sampling frequency (or half data) for reconstruction. SNR comparisons with different white noise levels show that these two trajectories have the same SNR character. In conclusion, the SP trajectory can reduce the aliasing artifact without decreasing SNR and also provide a way for undersampling recon- struction. Furthermore, this method can be applied to three-dimensional (3D) hybrid or spherical radial k-space sampling for a more efficient reduction of aliasing artifacts.展开更多
BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution...BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with "Curves", "Autocontrast", "Gray scale 8 bit", "Invert", "Image resize to 50%" steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.展开更多
文摘Through analysis on existing problems and rationality of East Street reconstruction project,its social benefit had been analyzed sysmatically and comprehensively from the aspects of social environment,natural environment and traffic safety environment.Furthermore,evaluation indexes of East Street reconstruction project had been established and weighting of each index had been identified by analytic hierarchy process(AHP).Fuzzy Synthetic Evaluation Model had been founded through fuzzy synthetic evaluation method.Finally,scientific judgment was conducted on social benefit of East Street reconstruction project by applying established evaluation system.It demonstrated that East Street reconstruction project could bring great social benefit,so it should support this project.
基金the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (Grant No. 2012BAD05B06)
文摘Fertile topsoil was added onto the surface of barren slope land in Three Gorges Reservoir region of China in an anthropogenic process known as the foreign soil reconstruction project. The main goal of this paper was to reveal the influence of anthropogenic activities on pedogenic processes and soil classifications. Chemical weathering indices and comparative analysis were applied to discuss changes in geochemical compositions and weathering features of purplish soils(Cambisols) before and after the project. Results showed that:(1) The foreign soil reconstruction project slightly altered the major element composition of topsoil and improved the soil structure. Although the distributions of major elements in the original topsoil, original subsoil, foreign topsoil and newly constructed topsoil were all similar to that in upper continental crust, newly constructed topsoil was the most similar soil.(2) The chemical index of alteration was more sensitive than the weathering index of Parker at indicating chemical weathering status of purplish soil. The chemical weathering status of newly constructed topsoil was higher than that of the original topsoil and lower than that of foreign topsoil.(3) Anthropogenic activities may provide a promising new thought for the anthropogenic soil classification system. The scope and subgroups of Anthrosols should be extended and strengthened. Or there may be a need to combine Anthrosols and Technosols orders to create a new soil order. The results may be used for optimizing soil mellowing engineering activities and enriching the soil classification system.
文摘Aba Prefecture Company Name:Sichuan Xintong New Materials Co.Ltd. Address:Shuimo,Wenchuan Nature of Business:Private Project Name:Processing and Sale of Tourism Products Details:Producing and processing of Natural crystal products,man-made gem and other
基金Project supported by the National Basic Research Program of China(Grant No.2006CB7057005)the National High Technology Research and Development Program of China(Grant No.2009AA012200)the National Natural Science Foundation of China (Grant No.60672104)
文摘With the development of the compressive sensing theory, the image reconstruction from the projections viewed in limited angles is one of the hot problems in the research of computed tomography technology. This paper develops an iterative algorithm for image reconstruction, which can fit the most cases. This method gives an image reconstruction flow with the difference image vector, which is based on the concept that the difference image vector between the reconstructed and the reference image is sparse enough. Then the l1-norm minimization method is used to reconstruct the difference vector to recover the image for flat subjects in limited angles. The algorithm has been tested with a thin planar phantom and a real object in limited-view projection data. Moreover, all the studies showed the satisfactory results in accuracy at a rather high reconstruction speed.
基金Sponsored by Project of Ning Xia Hui Autonormous Region Communications Department2005
文摘Criterion of differential settlement in subgrade is useful for keeping road in a good serving condition. The theory of traditional materials strength and their technologic economy are applied to analyze the additional bend stress of the pavement structure, the equivalent beam of elastic foundation and influence on the pavement structure of an uneven settlement. The allowable settling ratio of pavement index ( △i ) is got and is used to appraise the uneven settlement standard value in subgrade. The allowable settling ratio of pavement index is applied in an certain widening expressway project and △i ≤0.36 % is attained by the analyzing, calculating and adding a certain safe coefficient. This value was put into practice and is effective during the past two years.
基金Project supported by the Young Scientists Fund of the National Natural Science Foundation of China(Grant No.61205151)the National Key Scientific Instrument and Equipment Development Project of China(Grant No.2014YQ060537)the National Basic Research Program,China(Grant No.2013CB632803)
文摘An improved algebraic reconstruction technique(ART) combined with tunable diode laser absorption spectroscopy(TDLAS) is presented in this paper for determining two-dimensional(2D) distribution of H2O concentration and temperature in a simulated combustion flame.This work aims to simulate the reconstruction of spectroscopic measurements by a multi-view parallel-beam scanning geometry and analyze the effects of projection rays on reconstruction accuracy.It finally proves that reconstruction quality dramatically increases with the number of projection rays increasing until more than 180 for 20 × 20 grid,and after that point,the number of projection rays has little influence on reconstruction accuracy.It is clear that the temperature reconstruction results are more accurate than the water vapor concentration obtained by the traditional concentration calculation method.In the present study an innovative way to reduce the error of concentration reconstruction and improve the reconstruction quality greatly is also proposed,and the capability of this new method is evaluated by using appropriate assessment parameters.By using this new approach,not only the concentration reconstruction accuracy is greatly improved,but also a suitable parallel-beam arrangement is put forward for high reconstruction accuracy and simplicity of experimental validation.Finally,a bimodal structure of the combustion region is assumed to demonstrate the robustness and universality of the proposed method.Numerical investigation indicates that the proposed TDLAS tomographic algorithm is capable of detecting accurate temperature and concentration profiles.This feasible formula for reconstruction research is expected to resolve several key issues in practical combustion devices.
基金This project is supported by National Natural Science Foundation ofChina (No.50375047).
文摘A fast 3D reconstruction method based on structured light to measure various parameters of the raceway groove is presented. Digital parallel grating stripes distributed with sine density are projected onto the raceway groove by a DLP projector, and distorting of stripes is happened on the raceway. Simultaneously, aided by three-step phase-shifting approach, three images covered by different stripes are obtained by a high-resolution CCD camera at the same location, thus a more accuracy local topography can be obtained. And then the bearing is rotated on a high precision computer controlled rotational stage. Three images are also obtained as the former step at next planned location triggered by the motor. After one cycle, all images information is combined through the mosaics. As a result, the 3D information of raceway groove can be gained. Not only geometric properties but also surface flaws can be extracted by software. A preliminary hardware system has been built, with which some geometric parameters have been extracted from reconstructed local topography.
基金Supported by National Key Technology R&D Program of the Ministry of Science and Technology(No.2012BAI07B05)
文摘CBCT scanners have been widely used in angiography,radiotherapy guidance,mammography and oral maxillofacial imaging.To cut detector size,reduce manufacturing costs and radiation dose while keeping a reasonable FOV,the flat panel detector can be placed off-center horizontally.This scanning configuration extends the FOV effectively.However,each projection is transversely truncated,bringing errors and artifacts in reconstruction.In this paper,a simple but practical method is proposed for this scanning geometry based on truncation compensation and the modified FDK algorithm.Numerical simulations with jaw phantom were conducted to evaluate the accuracy and practicability of the proposed method.A novel CBCT system for maxillofacial imaging is used for clinical test,which is equipped with an off-center small size flat panel detector.Results show that reconstruction accuracy is acceptable for clinical use,and the image quality appears sufficient for specific diagnostic requirements.It provides a novel solution for clinical CBCT system,in order to reduce radiation dose and manufacturing cost.
文摘Data collected in two-dimensional projections give planar images of object at each projection angle. To obtain information along the depth of the object, tomographic images are reconstructed using these projections. There are basically two approaches to solve the problem of reconstruction: analytical and iterative, each one presenting its own advantages and limitations. This paper provides a detailed introduction and comparison to four analytical image reconstruction methods including Fourier transformation, simple back-projection, back-projection filtering and filtered back-projection.
基金The National Natural Science Foundation ofChina (No30570511)
文摘To accelerate the scan speed and improve the image quality, a new type of CT configuration, "double-source double-multislice spiral CT" (DSDMS-CT), which is based on two sets of single-source multislice spiral CT was proposed with a special reconstruction algorithm. Simulation results using the fan-beam filtered backprojection algorithm with a special interpolation method were presented for both single-source multislice spiral CT and DSDMS-CT. The results of new CT model show that it scans faster than the traditional spiral CT and has a better slice sensitivity profile (SSP) with larger pitch value.
文摘In the process of reconstruction and extension of highways,the most important thing is to ensure that the overall level of the widening of interchanges is comprehensively improved to ensure safe traffic.However,due to the heavy and difficult tasks in the widening of interchanges,higher requirements are put forward for the construction quality of the project.In the actual construction of the project,it is necessary to actively analyze and explain the specific construction of the interchange widening project to ensure that the improvement level of the interchange widening in highway reconstruction and extension project is effectively upgraded.This paper gives a detailed introduction to the main construction technology for interchange widening project,clarifies the technical points of safety and guaranteed access,and proposes corresponding safeguard measures to ensure the comprehensive upgrade of the construction quality and standard of interchange widening in highway reconstruction and extension.
基金Supported by the Hi-Tech Research and Development Program of China (No. 2011AA120102)
文摘A fast converging sparse reconstruction algorithm in ghost imaging is presented. It utilizes total variation regularization and its formulation is based on the Karush-Kuhn-Tucker (KKT) theorem in the theory of convex optimization. Tests using experimental data show that, compared with the algorithm of Gradient Projection for Sparse Reconstruction (GPSR), the proposed algorithm yields better results with less computation work.
基金Project supported by the National Basic Research Program of China(Grant No.2011CB707701)the Innovation Fund for Technology Based Firms,China(Grant No.11C26221103870)+1 种基金the National High Technology Research and Development Program of China(Grant Nos.2011BAI12B05 and 2011BAI23B07)the National Natural Science Foundation of China(Grant Nos.81171330,81271664,and 81230035)
文摘Radial imaging techniques, such as projection-reconstruction (PR), are used in magnetic resonance imaging (MRI) for dynamic imaging, angiography, and short-T2 imaging. They are less sensitive to flow and motion artifacts, and support fast imaging with short echo times. However, aliasing and streaking artifacts are two main sources which degrade radial imaging quality. For a given fixed number of k-space projections, data distributions along radial and angular directions will influence the level of aliasing and streaking artifacts. Conventional radial k-space sampling trajectory introduces an aliasing artifact at the first principal ring of point spread function (PSF). In this paper, a shaking projection (SP) k-space sampling trajectory was proposed to reduce aliasing artifacts in MR images. SP sampling trajectory shifts the projection alternately along the k-space center, which separates k-space data in the azimuthal direction. Simulations based on conventional and SP sampling trajectories were compared with the same number projections. A significant reduction of aliasing artifacts was observed using the SP sampling trajectory. These two trajectories were also compared with different sampling frequencies. ASP trajectory has the same aliasing character when using half sampling frequency (or half data) for reconstruction. SNR comparisons with different white noise levels show that these two trajectories have the same SNR character. In conclusion, the SP trajectory can reduce the aliasing artifact without decreasing SNR and also provide a way for undersampling recon- struction. Furthermore, this method can be applied to three-dimensional (3D) hybrid or spherical radial k-space sampling for a more efficient reduction of aliasing artifacts.
基金Natural Science Research Plan forJiangsu Colleges, No.05KJB180105 Postgraduate Innovation Cultivating Projectin Jiangsu Province, No.CX07s_035z
文摘BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with "Curves", "Autocontrast", "Gray scale 8 bit", "Invert", "Image resize to 50%" steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.