The erythrocytes or red blood cells(RBCs)and leucocytes or white blood cells(WBCs)are the most abundant cell types in the circulatory system of vertebrates.The RBCs are responsible for gas exchange while WBCs are sole...The erythrocytes or red blood cells(RBCs)and leucocytes or white blood cells(WBCs)are the most abundant cell types in the circulatory system of vertebrates.The RBCs are responsible for gas exchange while WBCs are solely involved with immunity.In this study,we present evidence that fish RBCs despite their respiratory role they also express immune-related genes and responses in the teleost fish,Nile tilapia(Oreochromis niloticus).Fluorescent staining identified the presence of numerous mitochondria and lysosomes in tilapia RBCs.RNA sequencing of tilapia RBCs and WBCs led to the assembly and annotation of 20.876 transcripts of which 1017 and 3628 transcripts were specific to RBCs and WBCs,respectively.Pairwise sequence comparisons identified 3251 transcripts that were significantly differential expressed of which 707(21.75%)were up-regulated and 2544(78.25%)down-regulated in RBCs when compared to WBCs.809 transcripts were assigned to 16 immune system pathways,21 and 163 transcripts were specifically expressed in tilapia RBCs and WBCs respectively,and 23 and 233 transcripts were highly expressed in RBCs and WBCs respectively.Expression of 7 immune genes(interferon regulatory factors(irf)1e5 and 9 and tlr3)in both WBCs and RBCs was validated by RT-PCR analysis.Quantitative real-time PCR revealed that all genes were up-regulated in poly(I:C)-challenged WBCs and irf(1,3,4,and 9)and tlr3 were also significantly up-regulated in poly(I:C)-challenged RBCs.Therefore,fish RBCs expressed immunity genes and responses and they may play a complementary role in vertebrate immunity.展开更多
BACKGROUND Acute kidney injury(AKI)after liver transplantation(LT)is a frequent and multifactorial event related to increased morbidity and mortality.Risk factors for AKI after LT still need to be clarified.AIM To ide...BACKGROUND Acute kidney injury(AKI)after liver transplantation(LT)is a frequent and multifactorial event related to increased morbidity and mortality.Risk factors for AKI after LT still need to be clarified.AIM To identify the predictors of acute kidney injury after liver transplantation.METHODS The frequency and pre-and intraoperative predictors of AKI within the first 7 d after LT were evaluated in adult liver transplant candidates in a single LT center in Croatia.AKI was defined according to the Kidney Disease:Improving Global Outcomes criteria.RESULTS Out of 205 patients(mean age 57±10 years;73.7%males,52.7%with alcoholrelated liver disease)93(45.36%)developed AKI,and the majority of them(58.06%)had stage 1.Only 5.38%of patients required renal replacement therapy after LT.The majority of patients(82.8%)developed AKI within the first two days after the procedure.Multivariate logistic regression identified pre-LT body mass index(OR=1.1,95%CI:1.05-1.24)and red blood cell transfusion(OR=1.66,95%CI:1.09-2.53)as independent predictors of early post-LT AKI occurrence.30-d survival after LT was significantly better for patients without AKI(P=0.01).CONCLUSION Early AKI after LT is a frequent event that negatively impacts short-term survival.The pathogenesis of AKI is multifactorial,but pre-LT BMI and intraoperative volume shifts are major contributors.展开更多
基金This work was supported by China Agriculture Research System(CARS-46)Shanghai Collaborate Innovation Center for Aquatic Animal Genetics and Breeding(ZF1206)by the Open Fund of Key Laboratory of Experimental Marine Biology from the Chinese Academy of Sciences(No.KF2017NO2).
文摘The erythrocytes or red blood cells(RBCs)and leucocytes or white blood cells(WBCs)are the most abundant cell types in the circulatory system of vertebrates.The RBCs are responsible for gas exchange while WBCs are solely involved with immunity.In this study,we present evidence that fish RBCs despite their respiratory role they also express immune-related genes and responses in the teleost fish,Nile tilapia(Oreochromis niloticus).Fluorescent staining identified the presence of numerous mitochondria and lysosomes in tilapia RBCs.RNA sequencing of tilapia RBCs and WBCs led to the assembly and annotation of 20.876 transcripts of which 1017 and 3628 transcripts were specific to RBCs and WBCs,respectively.Pairwise sequence comparisons identified 3251 transcripts that were significantly differential expressed of which 707(21.75%)were up-regulated and 2544(78.25%)down-regulated in RBCs when compared to WBCs.809 transcripts were assigned to 16 immune system pathways,21 and 163 transcripts were specifically expressed in tilapia RBCs and WBCs respectively,and 23 and 233 transcripts were highly expressed in RBCs and WBCs respectively.Expression of 7 immune genes(interferon regulatory factors(irf)1e5 and 9 and tlr3)in both WBCs and RBCs was validated by RT-PCR analysis.Quantitative real-time PCR revealed that all genes were up-regulated in poly(I:C)-challenged WBCs and irf(1,3,4,and 9)and tlr3 were also significantly up-regulated in poly(I:C)-challenged RBCs.Therefore,fish RBCs expressed immunity genes and responses and they may play a complementary role in vertebrate immunity.
基金Supported by the Croatian Science Foundation grant“The Role of Notch Signalling Pathway in Pathogenesis of Hepatic Fibrosis”,No.UIP-2017-05-1965.
文摘BACKGROUND Acute kidney injury(AKI)after liver transplantation(LT)is a frequent and multifactorial event related to increased morbidity and mortality.Risk factors for AKI after LT still need to be clarified.AIM To identify the predictors of acute kidney injury after liver transplantation.METHODS The frequency and pre-and intraoperative predictors of AKI within the first 7 d after LT were evaluated in adult liver transplant candidates in a single LT center in Croatia.AKI was defined according to the Kidney Disease:Improving Global Outcomes criteria.RESULTS Out of 205 patients(mean age 57±10 years;73.7%males,52.7%with alcoholrelated liver disease)93(45.36%)developed AKI,and the majority of them(58.06%)had stage 1.Only 5.38%of patients required renal replacement therapy after LT.The majority of patients(82.8%)developed AKI within the first two days after the procedure.Multivariate logistic regression identified pre-LT body mass index(OR=1.1,95%CI:1.05-1.24)and red blood cell transfusion(OR=1.66,95%CI:1.09-2.53)as independent predictors of early post-LT AKI occurrence.30-d survival after LT was significantly better for patients without AKI(P=0.01).CONCLUSION Early AKI after LT is a frequent event that negatively impacts short-term survival.The pathogenesis of AKI is multifactorial,but pre-LT BMI and intraoperative volume shifts are major contributors.
