Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethyl...Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)method.Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold-based immunochromatographic assay(CG-ICA)methods were used to determine R2G in fruit drinks,red wine,and yoghurts.A standard curve of the developed ic-ELISA showed that the IC50 of the anti-R2G mAb was 1.02 ng/mL,and limit of detection value(LOD)was 0.21 ng/mL.For the CG-ICA developed,the visual limit of detection values(vLOD)were 2 ng/mL and cut-off values of 100 ng/mL in samples.The results indicated that these two methods could be used to quickly detect R2G in fruit drinks,red wine,and yoghurts.展开更多
建立快速测定食品中的非法添加色素红2G的固相萃取超高效液相色谱分析方法。选取可能添加红2G的香肠、红酒、红醋、糖果、年糕、牛肉干、海鲜酱、辣椒酱、胡椒粉、辣椒粉、辣椒油、火锅底料作为分析对象,样品用50%(体积分数)甲醇水溶液...建立快速测定食品中的非法添加色素红2G的固相萃取超高效液相色谱分析方法。选取可能添加红2G的香肠、红酒、红醋、糖果、年糕、牛肉干、海鲜酱、辣椒酱、胡椒粉、辣椒粉、辣椒油、火锅底料作为分析对象,样品用50%(体积分数)甲醇水溶液提取,经固相萃取小柱净化,以含乙酸(0.02 mo L/L)的乙酸铵(0.01 mo L/L)水溶液和甲醇为流动相,经C18色谱柱分离,二极管阵列检测器检测,检测波长508 nm。红2G在0.2 mg/L^100 mg/L浓度范围内呈良好的线性关系,相关系数为1.000 0,方法检出限为0.03 mg/kg,样品平均回收率在80%以上,相对标准偏差小于5%(n=6)。该方法灵敏度高,精密度好,分析时间短,适用于食品中非法添加色素红2G的检测。展开更多
基金This work was funded by the National Key R&D Program(2019YFC1604602)grants from Science and Technology Program of Jiangsu Market Supervision and Administration Bureau(KJ21125093)This work was also supported by the Fundamental Research Funds for the Central Universities(JUSRP622012).
文摘Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)method.Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold-based immunochromatographic assay(CG-ICA)methods were used to determine R2G in fruit drinks,red wine,and yoghurts.A standard curve of the developed ic-ELISA showed that the IC50 of the anti-R2G mAb was 1.02 ng/mL,and limit of detection value(LOD)was 0.21 ng/mL.For the CG-ICA developed,the visual limit of detection values(vLOD)were 2 ng/mL and cut-off values of 100 ng/mL in samples.The results indicated that these two methods could be used to quickly detect R2G in fruit drinks,red wine,and yoghurts.
文摘建立快速测定食品中的非法添加色素红2G的固相萃取超高效液相色谱分析方法。选取可能添加红2G的香肠、红酒、红醋、糖果、年糕、牛肉干、海鲜酱、辣椒酱、胡椒粉、辣椒粉、辣椒油、火锅底料作为分析对象,样品用50%(体积分数)甲醇水溶液提取,经固相萃取小柱净化,以含乙酸(0.02 mo L/L)的乙酸铵(0.01 mo L/L)水溶液和甲醇为流动相,经C18色谱柱分离,二极管阵列检测器检测,检测波长508 nm。红2G在0.2 mg/L^100 mg/L浓度范围内呈良好的线性关系,相关系数为1.000 0,方法检出限为0.03 mg/kg,样品平均回收率在80%以上,相对标准偏差小于5%(n=6)。该方法灵敏度高,精密度好,分析时间短,适用于食品中非法添加色素红2G的检测。
