In this study, we conducted a cross of white crucian carp(♀)×red crucian carp(♂)(WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color s...In this study, we conducted a cross of white crucian carp(♀)×red crucian carp(♂)(WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate(90.2%) and high hatchery rate(80.5%), which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes(2n=100). Amplified ITS of 45 S r DNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S r DNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.展开更多
为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish...为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish）、水泡眼（Water vesicle goldfish）、珍珠（Genuine pearl goldfish）的线粒体DNA上细胞色素b的部分核苷酸序列，长度为597bp。结合GenBank中红鲫、野鲫、日本白鲫、银鲫、鲤鱼的序列进行比较分析，结果显示，这7个金鱼品种之间的同源性都很高，在99．5％-100％之间；7种金鱼和红鲫的同源性也很高，为99．5％～99．8％，与野鲫的同源性在96．8％～97．2％，与日本白鲫、银鲫的同源性为93．1％～94．3％，与鲤鱼的同源性相对较低，为88．3％-88．6％。利用DNAstar软件构建了不同品种金鱼和鲫鱼的分子系统树，从分子水平进一步证实了金鱼起源于野鲫。展开更多
基金supported by Major International Cooperation Projects of the National Natural Science Foundation of China(31210103918)the National Natural Science Foundation of China(31201980,31430088 and 31272651)+5 种基金the National High Technology Research and Development Program of China(2011AA100403)the Natural Science Foundation of Hunan Province,China(14JJ3072)Science-Technology Foundation of Hunan Province,China(2014FJ3084)Research Foundation of Education Bureau of Hunan Province,China(13C523)the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486)the Construct Program of the Key Discipline in Hunan Province,and Construct Program of the National Key Discipline
文摘In this study, we conducted a cross of white crucian carp(♀)×red crucian carp(♂)(WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate(90.2%) and high hatchery rate(80.5%), which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes(2n=100). Amplified ITS of 45 S r DNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S r DNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.
文摘为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish）、水泡眼（Water vesicle goldfish）、珍珠（Genuine pearl goldfish）的线粒体DNA上细胞色素b的部分核苷酸序列，长度为597bp。结合GenBank中红鲫、野鲫、日本白鲫、银鲫、鲤鱼的序列进行比较分析，结果显示，这7个金鱼品种之间的同源性都很高，在99．5％-100％之间；7种金鱼和红鲫的同源性也很高，为99．5％～99．8％，与野鲫的同源性在96．8％～97．2％，与日本白鲫、银鲫的同源性为93．1％～94．3％，与鲤鱼的同源性相对较低，为88．3％-88．6％。利用DNAstar软件构建了不同品种金鱼和鲫鱼的分子系统树，从分子水平进一步证实了金鱼起源于野鲫。
