Formulation and Evaluation of Lipstick with Betacyanin Pigment of <i>Hylocereus polyrhizus</i>(Red Dragon Fruit)

Lipstick is the most widely used cosmetic product. Although lipstick gives a lot of social, psychological and therapeutic benefits, it may harm the consumers. Because most lipsticks contained high lead level and long term use of high lead level lipstick may harm the consumers. Research findings have suggested that administration of various antioxidants can prevent or subdue various toxic effects of lead and generation of oxidative stress. Hylocereus polyrhizus (Red dragon fruit) is widely available in Myanmar and it contains powerful antioxidant amaranth colorant called betacyanin pigment. It can prevent the oxidative stress caused by low level of lead, thus suitable as natural colorant for lipstick. As a role of pharmacist, this research was intended to minimize the adverse effects of lipsticks by formulating natural lipstick with betacyanin pigment obtained from H. polyrhizus and other natural ingredients. Formulation of lipstick was carried out by heating and blending method with homogenizer at a speed of 12000 rpm. Then, pH, melting point, surface abnormalities, aging stability, perfume stability and antioxidant activity were performed as quality evaluation. Skin irritation test, microbial analysis and lead content determination were carried out as safety evaluation. Formulated lipsticks with betacyanin pigment of H. polyrhizus have acceptable quality. The IC50 of standard ascorbic acid and formulated lipstick were 4.51 μg/ml and 22.23 μg/ml respectively. In quantitative analysis for microbiological control, formulated lipstick had no visible colony and in qualitative analysis, Staphylococcus aureus, E. coli and Pseudomonas aeruginosa were not detected in 0.5 g of formulated lipsticks. Moreover, lead content of formulated lipstick was only 2.9 ppm that is within allowable limit and it had negligible skin irritancy. Therefore, lipstick with betacyanin pigment of H. polyrhizus can utilize as Cosmeceutical.

Dissipation pattern of azoxystrobin and difenoconazole in red dragon fruit(Hylocereus polyrhizus)cultivated in Indonesian highland(West Java)and coastal area(D.I.Jogyakarta)and its implication for dietary risk assessment

Background:Cultivation of red dragon fruit(Hylocereus polyrhizus)in Indonesian orchards is hampered by anthracnose and stem canker.A mixture of azoxystrobin 200 g/L+difenoconazole 125 g/L is recommended in supporting the obligated integrated pest management.For the consumer safety,dietary intake of the residues from fresh edible part should be assessed;therefore,information on correct dissipation pattern of the applied pesticide is required.Materials and Methods:Field residue trials in Indonesian highland(West Java)and coastal area(Special Region of Yogyakarta)were done at recommended dose was 0.151 kg ai/ha azoxystrobin and 0.094 kg ai/ha difenoconazole,three times at 10 days interval.Whole fruit samples were collected at 70%-100%ripeness at−1,0,1,3,7,10,and 14 days after last application,processed and determined its residue as soon as possible.Result and Discussion:Photoisomerization of azoxystrobin was observed at Day 0,especially in coastal area;however,it has been dissipated to below lowest validated level in the following day.At Day 0,3%-5%of the deposited azoxystrobin and difenoconazole penetrate into the flesh;however,most residues(95%-97%)were retained in peel,and dissipated in prolonged day.The dissipation pattern was non-linear.The dissipation data were better fit with bi-exponential double-first-order in parallel than single first-order kinetics model.The DT50 of both azoxystrobin and difenoconazole was 3 days.At harvest time,seventh day,only azoxystrobin residue was detected in flesh at 0.006 mg/kg;therefore,the long-term dietary risk was 0 per cent acceptable daily intake.Conclusion:Fresh red dragon fruit is safe to consume.