Giant red sea cucumber (Parastichopus californicus) is an under-utilized species due to its high tendency to autolysis.The aim of this study was to evaluate the functional properties of collagen hydrolysates from this...Giant red sea cucumber (Parastichopus californicus) is an under-utilized species due to its high tendency to autolysis.The aim of this study was to evaluate the functional properties of collagen hydrolysates from this species.The degree of hydrolysis (DH),amino acid composition,SDS-PAGE,emulsion activity index (EAI),emulsion stability index (ESI),foam expansion (FE),and foam stability (FS) of hydrolysates were investigated.The effects of pH on the EAI,ESI FE and FS of hydrolysates were also inves-tigated.The results indicated that the β and α1 chains of the collagen were effectively hydrolyzed by trypsin at 50℃ with an En-zyme/Substrate (E/S) ration of 1:20 (w:w).The DH of collagen was up to 17.3% after 3 h hydrolysis with trypsin.The hydrolysates had a molecular weight distribution of 1.1 17 kDa,and were abundant in glycine (Gly),proline (Pro),glutamic acid (Glu),alanine (Ala) and hydroxyproline (Hyp) residues.The hydrolysates were fractionated into three fractions (< 3 kDa,3 10 kDa,and > 10 kDa),and the fraction of 3 10 kDa exhibited a higher EAI value than the fraction of > 10 kDa (P<0.05).The fraction of > 10 kDa had higher FE and FS values than other fractions (P<0.05).The pH had an important effect on the EAI,ESI,FE and FS.All the fractions showed undesirable emulsion and forming properties at pH 4.0.Under pH 7.0 and pH 10.0,the 3 10 kDa fraction showed higher EAI value and the fraction of > 10 kDa showed higher FE value,respectively.They are hoped to be utilized as functional ingredients in food and nutraceutical industries.展开更多
目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资...目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资料、手术前后Hb和Plt、住院时间、术中出血量、术中自体血回收量、术前自体浓缩红细胞和血小板采集量、术中异体红细胞、血浆及血小板的输注量。结果 1)2组患者基线水平一致,手术前后Hb和Plt、住院时间均无统计学差异(P>0.05);2)深度自体组和对照组患者术中出血量分别为2 000 mL(1 200,3 500)vs 2 600 mL(1 000,3 875)(P>0.05),术中自体血回收量为44 U vs 9 U;3)2组术前自体浓缩红细胞和自体血小板采集总量分别为68 U vs 4 U、17 U vs 0 U;4)2组围手术期异体红细胞、血浆和血小板的输注总量分别为18 U vs 53 U、24 U vs 66 U、2 U vs 4 U;5)深度自体组有5例患者避免了异体血输注,占55.6%(5/9)。结论单采深度自体储血技术能在短时间内获得大量的自体红细胞和自体血小板,具备避免特大择期手术患者使用异体红细胞和异体血小板的巨大潜力。展开更多
基金supported by the National Key Technology Research and Development Program of China (Grant No.2006BAD30B01)the Research Award Fund for Excellent Young Scientist of Shandong Province,China (Grant No.BS2009HZ005)
文摘Giant red sea cucumber (Parastichopus californicus) is an under-utilized species due to its high tendency to autolysis.The aim of this study was to evaluate the functional properties of collagen hydrolysates from this species.The degree of hydrolysis (DH),amino acid composition,SDS-PAGE,emulsion activity index (EAI),emulsion stability index (ESI),foam expansion (FE),and foam stability (FS) of hydrolysates were investigated.The effects of pH on the EAI,ESI FE and FS of hydrolysates were also inves-tigated.The results indicated that the β and α1 chains of the collagen were effectively hydrolyzed by trypsin at 50℃ with an En-zyme/Substrate (E/S) ration of 1:20 (w:w).The DH of collagen was up to 17.3% after 3 h hydrolysis with trypsin.The hydrolysates had a molecular weight distribution of 1.1 17 kDa,and were abundant in glycine (Gly),proline (Pro),glutamic acid (Glu),alanine (Ala) and hydroxyproline (Hyp) residues.The hydrolysates were fractionated into three fractions (< 3 kDa,3 10 kDa,and > 10 kDa),and the fraction of 3 10 kDa exhibited a higher EAI value than the fraction of > 10 kDa (P<0.05).The fraction of > 10 kDa had higher FE and FS values than other fractions (P<0.05).The pH had an important effect on the EAI,ESI,FE and FS.All the fractions showed undesirable emulsion and forming properties at pH 4.0.Under pH 7.0 and pH 10.0,the 3 10 kDa fraction showed higher EAI value and the fraction of > 10 kDa showed higher FE value,respectively.They are hoped to be utilized as functional ingredients in food and nutraceutical industries.
文摘目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资料、手术前后Hb和Plt、住院时间、术中出血量、术中自体血回收量、术前自体浓缩红细胞和血小板采集量、术中异体红细胞、血浆及血小板的输注量。结果 1)2组患者基线水平一致,手术前后Hb和Plt、住院时间均无统计学差异(P>0.05);2)深度自体组和对照组患者术中出血量分别为2 000 mL(1 200,3 500)vs 2 600 mL(1 000,3 875)(P>0.05),术中自体血回收量为44 U vs 9 U;3)2组术前自体浓缩红细胞和自体血小板采集总量分别为68 U vs 4 U、17 U vs 0 U;4)2组围手术期异体红细胞、血浆和血小板的输注总量分别为18 U vs 53 U、24 U vs 66 U、2 U vs 4 U;5)深度自体组有5例患者避免了异体血输注,占55.6%(5/9)。结论单采深度自体储血技术能在短时间内获得大量的自体红细胞和自体血小板,具备避免特大择期手术患者使用异体红细胞和异体血小板的巨大潜力。
