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Unraveling engineering disturbance effects on deformation in red-bed mudstone railway cuttings:incorporating crack-facilitated moisture diffusion
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作者 HUANG Kang DAI Zhangjun +3 位作者 YAN Chengzeng YAO Junkai CHI Zecheng CHEN Shanxiong 《Journal of Mountain Science》 SCIE CSCD 2024年第5期1663-1682,共20页
Red-bed mudstone, prevalent in southwest China, poses a formidable challenge due to its hydrophilic clay minerals, resulting in expansion, deformation, and cracking upon exposure to moisture. This study addresses upli... Red-bed mudstone, prevalent in southwest China, poses a formidable challenge due to its hydrophilic clay minerals, resulting in expansion, deformation, and cracking upon exposure to moisture. This study addresses uplift deformation disasters in high-speed railways by employing a moisture diffusion-deformation-fracture coupling model based on the finite-discrete element method(FDEM). The model integrates the influence of cracks on moisture diffusion. The investigation into various excavation depths reveals a direct correlation between depth and the formation of tensile cracks at the bottom of the railway cutting. These cracks expedite moisture migration, significantly impacting the temporal and spatial evolution of the moisture field. Additionally, crack expansion dominates hygroscopic deformation, with the lateral coordinate of the crack zone determining peak vertical displacement. Furthermore, key factors influencing deformation in railway cuttings, including the swelling factor and initial moisture content at the bottom of the cutting, are explored. The number of tensile and shear cracks increases with greater excavation depth, particularly concerning shear cracks. Higher swelling factors and initial moisture contents result in an increased total number of cracks, predominantly shear cracks. Numerical calculations provide valuable insights, offering a scientific foundation and directional guidance for the precise prevention, control, prediction, and comprehensive treatment of mudstone-related issues in high-speed railways. 展开更多
关键词 red-bed mudstone Railway cutting FDEM Moisture diffusion DEFORMATION CRACK
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Swelling behaviors of heterogeneous red-bed mudstone subjected to different vertical stresses
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作者 Kang Huang Zhangjun Dai +3 位作者 Chengzeng Yan Fei Yu Wei Zhang Shanxiong Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1847-1863,共17页
In this study,the axial swelling strain of red-bed mudstone under different vertical stresses are measured by swell-under-load method,and the microstructure of mudstone after hygroscopic swelling is studied by mercury... In this study,the axial swelling strain of red-bed mudstone under different vertical stresses are measured by swell-under-load method,and the microstructure of mudstone after hygroscopic swelling is studied by mercury intrusion porosimetry(MIP).The weakening coefficient and Weibull distribution function are introduced into the coupling model of mudstone moisture diffusion-swelling deformation-fracture based on finite-discrete element method(FDEM).The weakening effect of moisture on mudstone's mechanical parameters,as well as the heterogeneity of swelling deformation and stress distribution,is considered.The microcrack behavior and energy evolution of mudstone during hygroscopic swelling deformation under different vertical stresses are studied.The results show that the axial swelling strain of mudstone decreases with increase of the vertical stress.At low vertical stresses,moisture absorption in mudstone leads to formation of cracks caused by hydration-induced expansion.Under high vertical stresses,a muddy sealing zone forms on the mudstone surface,preventing further water infiltration.The simulation results of mudstone swelling deformation also demonstrate that it involves both swelling of the mudstone matrix and swelling caused by crack expansion.Notably,crack expansion plays a dominant role in mudstone swelling.With increasing vertical stress,the cracks in mudstone change from tensile cracks to shear cracks,resulting in a significant reduction in the total number of cracks.While the evolution of mudstone kinetic energy shows similarities under different vertical stresses,the evolution of strain energy varies significantly due to the presence of different types of cracks in the mudstone.The findings provide a theoretical basis for understanding the hygroscopic swelling deformation mechanism of red-bed mudstone at various depths. 展开更多
关键词 Heterogeneous red-bed mudstone Self-sealing band Matric swelling and crack expansion FDEM MICROCRACKS Energy evolution
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贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响
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作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
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桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
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作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/rock信号通路
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
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芍药苷调节RhoA/ROCK信号通路对实验性自身免疫性葡萄膜炎小鼠Th17/Treg免疫平衡的影响 被引量:1
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作者 庞彬彬 陈震 邢怡桥 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第4期506-512,共7页
目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随... 目的探讨芍药苷调节Ras同源基因家族蛋白A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)小鼠辅助性T淋巴细胞17(Th17)/调节性T细胞(Treg)免疫平衡的影响。方法随机选出10只小鼠作为正常对照组,其余小鼠构建EAU模型,将造模成功小鼠随机平分为模型组、芍药苷组(10 mg·kg^(-1)芍药苷)、RhoA/ROCK信号通路激活剂溶血磷脂酸(LPA)组(25μmol·L^(-1)LPA)、芍药苷+LPA组(10 mg·kg^(-1)芍药苷+25μmol·L^(-1)LPA),每组10只。模型组和正常对照组给予等量生理盐水,给药每天1次,持续14 d。眼前节临床表现评分评估炎症严重程度;HE染色观察眼球组织病理形态;ELISA法检测血清中炎性因子转化生长因子-β(trarsforming growth factor beta,TGF-β)、白细胞介素10(IL-10)、白细胞介素17(IL-17)、白细胞介素23(IL-23)水平;流式细胞术检测脾脏Th17/Treg细胞水平;Western Blot法检测维甲酸相关核孤儿受体γt(RORγt)、叉头状转录因子3(Foxp3)以及RhoA/ROCK信号通路蛋白表达。结果正常对照组小鼠无虹膜、结膜血管舒张充血,与正常对照组比,模型组小鼠出现虹膜血管舒张充血,前房、虹膜、睫状体和视网膜内均有大量炎性细胞膨胀,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显升高(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显下降(P<0.05)。与模型组比,芍药苷组小鼠虹膜血管充血症状明显缓解,眼前节临床表现评分、IL-17和IL-23含量、Th17细胞水平、Th17/Treg、RORγt、RhoA、ROCK1蛋白水平明显降低(P<0.05),IL-10和TGF-β含量、Treg细胞水平、Foxp3水平明显升高(P<0.05);而LPA加重了眼部损伤(P<0.05),逆转了芍药苷对EAU小鼠的改善效果(P<0.05)。结论芍药苷可通过下调RhoA/ROCK信号通路调节EAU小鼠Th17/Treg免疫平衡减轻EAU小鼠葡萄膜炎。 展开更多
关键词 芍药苷 RhoA/rock信号通路 自身免疫性葡萄膜炎 TH17/TREG 小鼠
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艾灸联合保真汤加减治疗糖尿病肾病的疗效观察及对CT灌注参数、Rho/ROCK信号通路蛋白的影响 被引量:1
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作者 赵小艳 王俊杰 《上海针灸杂志》 CSCD 2024年第1期17-23,共7页
目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片... 目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片,观察组给予艾灸联合保真汤加减。观察两组中医主症积分、CT灌注参数[血尿素氮(blood urea nitrogen, BUN)、血肌酐(serum creatinine, SCr)、尿白蛋白排泄率(urinary albumin excretion rate, UAER)和24 h尿蛋白定量(24-hour urinary protein quantification, 24 h Upro)]、肾血流指标[舒张末期血流速度(end-diastolic velocity, EDV)、肾段动脉的收缩期峰值流速(peak-systolic velocity, PSV)、搏动指数(pulsatility index, PI)和阻力指数(resistive index, RI)]、血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-1β(interleukin-1β, IL-1β)、白细胞介素-6(interleukin-6, IL-6)和C反应蛋白(C-reactive protein, CRP)]水平及Rho/ROCK信号通路[Ras同源基因家族成员A(Ras homolog gene family member A, RhoA)、Rho关联含卷曲螺旋蛋白激酶Ⅰ(Rho-associated coiled-coil containing protein kinaseⅠ, ROCKI)、α-平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)和钙黏附蛋白-E(E-cadherin, E-Cad)]蛋白水平,并比较两组临床疗效及不良反应。结果 观察组总有效率为97.3%(72/74),明显高于对照组的81.9%(59/72)(P<0.05)。观察组治疗后中医主症积分低于治疗前和对照组(P<0.05)。两组治疗后CT灌注参数降低(P<0.05),且观察组低于对照组(P<0.05)。观察组治疗后PSV、EDV较治疗前和对照组加快(P<0.05),RI、PI较治疗前和对照组降低(P<0.05)。观察组治疗后血清炎性因子水平较治疗前和对照组降低(P<0.05)。观察组治疗后Rho A、ROCKI、α-SMA蛋白水平较治疗前和对照组降低(P<0.05),E-Cad蛋白水平较治疗前和对照组升高(P<0.05)。观察组不良反应发生率为1.4%(1/74),低于对照组的19.4%(14/72)(P<0.05)。结论 在对症治疗的基础上,艾灸联合保真汤加减可明显提高糖尿病肾病气阴两虚证患者的治疗效果,其机制可能与改善CT灌注参数,调节血清Rho/ROCK信号通路蛋白相关。 展开更多
关键词 灸法 糖尿病并发症 糖尿病肾病 气阴两虚 Rho/rock信号通路蛋白
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黄芩苷通过ROS依赖性调节RhoA/ROCK通路保护氯化钴诱导心肌细胞损伤的实验研究 被引量:2
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作者 沈艳玲 刘承红 +3 位作者 王世魁 徐尧 张云波 顾申红 《海南医学院学报》 CAS 北大核心 2024年第7期481-488,共8页
目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置... 目的:本研究旨在探讨黄芩苷(Baicalin)对氯化钴(CoCl_(2))诱导H9c2心肌细胞缺氧损伤的作用机制。方法:采用CoCl_(2)建立心肌细胞缺氧损伤模型,并分别加入不同浓度的黄芩苷培养。将正常氧培养设置为对照组,CoCl_(2)培养H9c2心肌细胞设置为CoCl_(2)组;Baicalin、Y27632(Rho激酶抑制剂)预处理的H9c2缺氧心肌细胞设置为CoCl_(2)+Baicalin组、CoCl_(2)+Y27632组、CoCl_(2)+Baicalin+Y27632组。通过细胞毒性检测试剂盒(CCK8)检测细胞活性;荧光探针测定细胞中活性氧(ROS)的表达;WST-8检测心肌细胞超氧化歧化酶(SOD)活力;TBA法测定丙二醇(MDA)浓度;同时采用WesternBlot方法分析RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达情况。结果:处理H9c2细胞24 h后,1 000μmol/L的CoCl_(2)和75μmol/L黄芩苷对治疗心肌细胞缺氧损伤具有较好的细胞活力。CoCl_(2)组细胞活性明显低于对照组,加入黄芩苷后可显著提高细胞活性(P<0.05);与对照组相比,CoCl_(2)组心肌细胞可上调ROS、MDA表达,下调SOD活力,升高RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,加入Baicalin后可逆转上诉蛋白的表达水平;与CoCl_(2)组相比,CoCl_(2)+Baicalin可抑制ROS、MDA的表达,上调SOD含量,下调RhoA、ROCK1、ROCK2、TNF-α、IL-1β蛋白表达水平,而加入Y27632(Rho激酶抑制剂)后则可显著增强Baicalin带来的保护作用。结论:Baicalin可减轻CoCl_(2)诱导H9c2心肌细胞缺氧损伤的炎症、氧化应激反应,其机制与抑制ROS依赖性RhoA/ROCK通路有关。 展开更多
关键词 黄芩苷 RhoA/Rho相关激酶(rock)信号通路 缺氧损伤模型
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益母草碱调节RhoA/ROCK信号通路对冠心病大鼠主动脉内皮细胞损伤的影响
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作者 郭艳红 谢月敏 +2 位作者 冯强 郭少英 王静 《河北医学》 CAS 2024年第8期1272-1279,共8页
目的:研究益母草碱(Leo)通过调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对冠心病(CHD)大鼠主动脉内皮细胞损伤的影响及机制。方法:将造模成功的75只CHD大鼠随机分为模型组(CHD组)、Leo低、中、高剂量组(Leo-... 目的:研究益母草碱(Leo)通过调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对冠心病(CHD)大鼠主动脉内皮细胞损伤的影响及机制。方法:将造模成功的75只CHD大鼠随机分为模型组(CHD组)、Leo低、中、高剂量组(Leo-L组、Leo-M组、Leo-H组)和Leo高剂量组+RhoA激活剂LPA组(Leo-H+LPA组),每组15只。Leo-L、Leo-M、Leo-H组分别灌胃15、30、60mg·kg^(-1)·d^(-1)的Leo,Leo-H+LPA组灌胃60mg·kg^(-1)·d^(-1)的Leo,另需腹腔注射1mg·kg^(-1)·d^(-1)的LPA,Control组和CHD组以相同方式给予等量生理盐水,均连续干预4周。超声心动图测定心功能指标;全自动生化分析仪测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;酶联免疫吸附测定(ELISA)检测白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、一氧化氮(NO)、内皮素1(ET-1)、内皮细胞特异性分子-1(EMS1)、血管内皮细胞粘附分子1(VCAM-1)水平;苏木精-伊红(HE)染色观察主动脉内皮损伤;流式细胞仪检测主动脉内皮细胞凋亡;免疫印迹法(Western blot)检测RhoA/ROCK信号通路相关蛋白表达。结果:与Control组相比,CHD组主动脉壁增厚且染色不均匀,细胞膨大,排列紊乱,内皮粗糙;左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、TC、TG、LDL-C、IL-6、TNF-α、ET-1、VCAM-1和EMS1水平、细胞凋亡率及RhoA、ROCK1和ROCK2蛋白表达显著升高(P<0.05),左室射血分数(LVEF)、缩短分数(FS)、HDL-C、NO水平显著降低(P<0.05)。与CHD组相比,Leo-L组、Leo-M组、Leo-H组主动脉染色均匀,动脉壁结构清晰,细胞排列紧密且形态较正常,内膜较平滑;LVEDD、LVESD、TC、TG、LDL-C、IL-6、TNF-α、ET-1、VCAM-1和EMS1水平、细胞凋亡率及RhoA、ROCK1和ROCK2蛋白表达逐渐降低(P<0.05),LVEF、FS、HDL-C、NO水平逐渐升高(P<0.05)。Leo-H+LPA组逆转了Leo对CHD大鼠的保护作用(P<0.05)。结论:Leo可能通过抑制RhoA/ROCK信号通路改善CHD大鼠心功能,降低血脂,抑制炎症,进而减轻CHD大鼠主动脉内皮细胞损伤。 展开更多
关键词 益母草碱 RhoA/rock信号通路 冠心病 内皮细胞损伤 血脂 炎症反应
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苍术素调节RhoA/ROCK信号通路对弥漫大B细胞淋巴瘤细胞恶性生物学行为的影响
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作者 张薇 陈姣敏 +2 位作者 许卫星 尹凤雷 王娟 《陕西医学杂志》 CAS 2024年第5期593-597,共5页
目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μ... 目的:探讨苍术素(ATR)调节Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞恶性生物学行为的影响。方法:常规培养DLBCL细胞SUDHL-4,将其随机分为对照组、低浓度ATR组(ATR-L组,5μmol/L ATR)、中浓度ATR组(ATR-M组,10μmol/L ATR)、高浓度ATR组(ATR-H组,20μmol/L ATR)和高浓度ATR+RhoA激活剂U46619组(ATR+U46619组,20μmol/L ATR+10 nmol/L U46619)。CCK-8法测定细胞增殖活性。划痕实验检测细胞迁移能力。Transwell实验测定细胞侵袭能力。流式细胞术检测细胞凋亡率。实时荧光定量PCR(RT-qPCR)法测定细胞中RhoA、ROCK1 mRNA表达。免疫印迹法(Western blot)测定各组细胞B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、RhoA、ROCK1蛋白表达。结果:与对照组比较,ATR-M组、ATR-H组SUDHL-4细胞OD450值(48、72 h)、细胞迁移率、细胞侵袭数目、RhoA、ROCK1 mRNA和蛋白表达、Bcl-2蛋白表达降低,细胞凋亡率、Bax蛋白表达升高(均P<0.05)。RhoA激活剂U46619减弱了ATR对DLBCL细胞恶性生物学行为的抑制作用。结论:ATR可能通过下调RhoA/ROCK信号通路抑制DLBCL细胞恶性生物学行为。 展开更多
关键词 弥漫大B细胞淋巴瘤 苍术素 RhoA/rock通路 细胞凋亡 细胞增殖 细胞侵袭 细胞迁移
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Drilling-based measuring method for the c-φ parameter of rock and its field application 被引量:3
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作者 Bei Jiang Fenglin Ma +5 位作者 Qi Wang Hongke Gao Dahu Zhai Yusong Deng Chuanjie Xu Liangdi Yao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期65-76,共12页
The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(R... The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(RCZ) is built. The formula for an ultimate cutting force is established based on the limit equilibrium principle. The relationship between digital drilling parameters(DDP) and the c-φ parameter(DDP-cφ formula, where c refers to the cohesion and φ refers to the internal friction angle) is derived, and the response of drilling parameters and cutting ratio to the strength parameters is analyzed. The drillingbased measuring method for the c-φ parameter of rock is constructed. The laboratory verification test is then completed, and the difference in results between the drilling test and the compression test is less than 6%. On this basis, in-situ rock drilling tests in a traffic tunnel and a coal mine roadway are carried out, and the strength parameters of the surrounding rock are effectively tested. The average difference ratio of the results is less than 11%, which verifies the effectiveness of the proposed method for obtaining the strength parameters based on digital drilling. This study provides methodological support for field testing of rock strength parameters. 展开更多
关键词 Digital drilling rock crushing zone c-u parameter Measurement method Field application
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Rho/ROCK通路与心律失常的研究进展
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作者 余意 杨波 《心血管病学进展》 CAS 2024年第7期577-580,共4页
越来越多的证据表明,Rho/ROCK通路在不同程度上调控着细胞的生长、发育、迁移、增殖和死亡,并在各种疾病中发挥重要作用。此外,Rho/ROCK通路与成纤维细胞、Ca^( 2+)和炎症因子等的相互作用对肺动脉高压和心血管疾病的发生与发展产生了... 越来越多的证据表明,Rho/ROCK通路在不同程度上调控着细胞的生长、发育、迁移、增殖和死亡,并在各种疾病中发挥重要作用。此外,Rho/ROCK通路与成纤维细胞、Ca^( 2+)和炎症因子等的相互作用对肺动脉高压和心血管疾病的发生与发展产生了重要的影响。近年来Rho/ROCK通路在心律失常中发挥的作用备受关注,现通过总结相关研究,为抗心律失常药的研究提供进一步参考。 展开更多
关键词 Rho/rock通路 心律失常 电生理
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地诺孕素配合腹腔镜治疗子宫内膜异位症的效果及对Rho/ROCK信号通路的影响
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作者 王冉 杨春燕 +2 位作者 谭琳玉 张清华 何芷若 《检验医学与临床》 CAS 2024年第12期1745-1749,共5页
目的探讨地诺孕素配合腹腔镜治疗子宫内膜异位症(EMT)的效果及对Rho/Rho激酶(ROCK)信号通路的影响。方法选取2020年6月至2022年6月湖南妇女儿童医院救治的80例EMT患者,按随机数字表法分为对照组(40例)和观察组(40例)。对照组采用单纯腹... 目的探讨地诺孕素配合腹腔镜治疗子宫内膜异位症(EMT)的效果及对Rho/Rho激酶(ROCK)信号通路的影响。方法选取2020年6月至2022年6月湖南妇女儿童医院救治的80例EMT患者,按随机数字表法分为对照组(40例)和观察组(40例)。对照组采用单纯腹腔镜+醋酸亮丙瑞林微球治疗,观察组采用地诺孕素配合腹腔镜+醋酸亮丙瑞林微球治疗。治疗6个月后对比两组的临床疗效及不良反应发生情况;比较治疗前及治疗6个月后两组性交痛、月经期间疼痛、非经期下腹疼痛的视觉模拟评分法(VAS),卵泡刺激素(FSH)、促黄体生成素(LH)、雌二醇(E2)水平,以及Rho/ROCK信号通路相关分子(RhoA、ROCKⅠ、ROCKⅡ)水平。结果观察组治疗总有效率为95.00%,高于对照组的85.00%,差异有统计学意义(P<0.05)。治疗前,两组性交痛、月经期间疼痛、非经期下腹疼痛的VAS评分比较,差异均无统计学意义(P>0.05);治疗6个月后,两组性交痛、月经期间疼痛、非经期下腹疼痛的VAS评分均较治疗前降低(P<0.05),且观察组性交痛、月经期间疼痛、非经期下腹疼痛的VAS评分均低于对照组(P<0.05)。治疗前,两组FSH、LH、E2水平比较,差异均无统计学意义(P>0.05);治疗6个月后,两组FSH、LH、E2水平均较治疗前降低(P<0.05),且观察组FSH、LH、E2水平均低于对照组(P<0.05)。治疗前,两组RhoA、ROCKⅠ、ROCKⅡ水平比较,差异均无统计学意义(P>0.05);治疗6个月后,两组RhoA、ROCKⅠ、ROCKⅡ水平均较治疗前降低(P<0.05),且观察组RhoA、ROCKⅠ、ROCKⅡ水平均低于对照组(P<0.05)。治疗6个月后,观察组的不良反应总发生率为5.00%,明显低于对照组的15.00%,差异有统计学意义(P<0.05)。结论地诺孕素配合腹腔镜治疗EMT有助于提高临床疗效,改善患者疼痛评分,降低患者雌激素水平,还可抑制Rho/ROCK信号通路相关因子的表达,降低不良反应发生率。 展开更多
关键词 地诺孕素 腹腔镜 子宫内膜异位症 RHO/rock信号通路 雌激素
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咖啡因通过FAK/AKT/ROCK通路调控人脑胶质瘤U-373MG细胞的恶性生物学行为
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作者 徐加志 张芸 +5 位作者 陈大刚 高风全 任德帅 吴卫东 杜妍 王娜 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第6期573-578,共6页
目的:探讨咖啡因通过调节FAK/AKT/ROCK信号通路来影响人脑胶质瘤U-373MG细胞的增殖、迁移和侵袭能力。方法:常规培养U-373MG细胞,将其分为对照组、咖啡因低剂量(1 mmol/L)组、咖啡因高剂量(2 mmol/L)组、PF573228组(FAK抑制剂,1μmol/L... 目的:探讨咖啡因通过调节FAK/AKT/ROCK信号通路来影响人脑胶质瘤U-373MG细胞的增殖、迁移和侵袭能力。方法:常规培养U-373MG细胞,将其分为对照组、咖啡因低剂量(1 mmol/L)组、咖啡因高剂量(2 mmol/L)组、PF573228组(FAK抑制剂,1μmol/L)、咖啡因高剂量+SC79组(AKT激活剂,8mg/L)。用CCK-8法、Transwell小室实验、流式细胞术和WB法分别检测各组U-373MG细胞的增殖、迁移、侵袭及凋亡,以及U-373MG细胞中p-FAK、p-AKT、p-ROCK、Ki67、MMP-9蛋白表达水平。建立U-373MG细胞裸鼠移植瘤模型,观察咖啡因对移植瘤生长的影响,WB法检测移植瘤组织中相关蛋白的表达。结果:咖啡因、PF573228可显著抑制U-373MG细胞的增殖、迁移、侵袭能力,促进U-373MG细胞凋亡,抑制p-FAK、p-AKT、p-ROCK、Ki67、MMP-9蛋白的表达(均P<0.05),SC79则可部分逆转咖啡因对U-373MG细胞的作用(均P<0.05)。咖啡因可显著抑制移植瘤的生长及移植瘤组织中上述相关蛋白的表达(均P<0.05)。结论:咖啡因可通过抑制FAK/AKT/ROCK信号通路抑制U-373MG细胞的增殖、迁移和侵袭能力,并促进其凋亡。 展开更多
关键词 咖啡因 人脑胶质瘤 U-373MG细胞 增殖 迁移 侵袭 凋亡 FAK/AKT/rock通路
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基于Rho/Rock通路研究麝香心痛宁抗大鼠血管损伤后内膜增生的机制研究
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作者 于凌群 高伟萍 +4 位作者 史明仟 辛新 王萌媛 曹桂云 杨莹 《药学研究》 CAS 2024年第3期232-235,共4页
目的观察麝香心痛宁抗大鼠血管损伤后内膜增生的作用及可能的作用机制。方法30只SD大鼠随机分为假手术组、模型对照组和麝香心痛宁组。采用球囊损伤法制备大鼠腹主动脉血管损伤模型,造模后灌胃给予大鼠麝香心痛宁片(200 mg·kg^(-1)... 目的观察麝香心痛宁抗大鼠血管损伤后内膜增生的作用及可能的作用机制。方法30只SD大鼠随机分为假手术组、模型对照组和麝香心痛宁组。采用球囊损伤法制备大鼠腹主动脉血管损伤模型,造模后灌胃给予大鼠麝香心痛宁片(200 mg·kg^(-1)),14 d后将大鼠处死,取其血清及腹主动脉,主动脉血管进行HE染色,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、单核细胞趋化因子-1(MCP-1)及谷胱甘肽过氧化物酶(GSH-Px)含量,ELISA法检测大鼠血管组织一氧化氮(NO)、内皮素-1(ET-1)、血管内皮生长因子(VEGF)和白细胞介素-1β(IL-1β)的含量,实时荧光定量PCR和Western blotting检测Rho、Rock1和Rock2 mRNA和蛋白的表达。结果与假手术组比较,模型对照组大鼠腹主动脉血管组织内膜增生,腹主动脉血管部位弹力变弱且管壁增厚。麝香心痛宁治疗后血管内膜损伤程度减轻。与模型对照组相比,麝香心痛宁组大鼠血清SOD、GSH-Px活性升高,MCP-1、MDA活性降低(P<0.05);血管组织NO含量升高,ET-1、VEGF和IL-1β含量降低(P<0.05);Rho/Rock通路相关因子Rock1、Rock2、Rho的表达降低(P<0.05)。结论麝香心痛宁可抑制血管损伤后内膜增生,其作用机制可能与抑制Rho/Rock信号通路有关。 展开更多
关键词 Rho/rock通路 麝香心痛宁 内膜增生 动脉球囊损伤
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炎调方调控Rho/ROCK信号通路对脓毒症急性胃肠损伤小鼠肠上皮细胞凋亡的研究
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作者 陈乾 王帝 +5 位作者 张定一 贾赛蕾 王丽辉 曹玉净 李鲜 李燕红 《中国比较医学杂志》 CAS 北大核心 2024年第2期16-24,共9页
目的探讨炎调方调控Rho/ROCK信号通路对脓毒症急性胃肠损伤小鼠肠上皮细胞凋亡的影响。方法取70只BALB/c小鼠随机分为空白组、假手术组和造模小鼠。除空白组、假手术组外,各组小鼠均采用盲肠结扎穿孔术(cecum ligation and puncture,CLP... 目的探讨炎调方调控Rho/ROCK信号通路对脓毒症急性胃肠损伤小鼠肠上皮细胞凋亡的影响。方法取70只BALB/c小鼠随机分为空白组、假手术组和造模小鼠。除空白组、假手术组外,各组小鼠均采用盲肠结扎穿孔术(cecum ligation and puncture,CLP)构建脓毒症急性胃肠损伤小鼠模型,将造模成功的小鼠随机分为模型组、炎调方低、中、高剂量组和ROCK抑制剂组。采用HE染色观察小鼠回肠组织病理学改变;ELISA法检测各组小鼠血清IL-1β、IL-6、TNF-α、IL-10水平;免疫组织化学检测观察PCNA、Ki-67的表达;蛋白印迹法检测回肠组织凋亡指标cleaved caspase3蛋白、Bax蛋白表达;RT-qPCR检测小鼠回肠组织ROCK mRNA、MLC mRNA表达。结果较空白组和假手术组相比,模型组小鼠的Chiu’s病理评分、促炎介质(IL-1β、IL-6、TNF-α)水平、cleaved caspase3蛋白、Bax蛋白、ROCK mRNA、MLC mRNA水平升高(P<0.05),而抑炎介质(IL-10)水平和回肠组织中PCNA、Ki-67表达降低(P<0.05)。与模型组比较,炎调方各组随着剂量增加,回肠组织病理学改变均得到不同程度改善,其Chiu’s病理评分、血清中IL-1β、IL-6、TNF-α水平、cleaved caspase3蛋白、Bax蛋白、ROCK mRNA、MLC mRNA的表达降低(P<0.05),而IL-10水平、回肠组织中PCNA、Ki-67表达升高(P<0.05)。结论炎调方可能通过调控Rho/ROCK信号通路抑制脓毒症急性胃肠损伤小鼠的肠上皮细胞凋亡,从而具有减轻肠道组织炎症反应,最终达到防止肠黏膜组织损伤的作用。 展开更多
关键词 脓毒症急性胃肠损伤 肠上皮细胞凋亡 RHO/rock信号通路 炎调方
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Mechanical properties and acoustic emission characteristics of soft rock with different water contents under dynamic disturbance 被引量:1
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作者 Yujing Jiang Lugen Chen +4 位作者 Dong Wang Hengjie Luan Guangchao Zhang Ling Dong Bin Liang 《International Journal of Coal Science & Technology》 EI CAS CSCD 2024年第3期135-148,共14页
Uniaxial compression tests and cyclic loading acoustic emission tests were conducted on 20%,40%,60%,80%,dry and saturated muddy sandstone by using a creep impact loading system to investigate the mechanical properties... Uniaxial compression tests and cyclic loading acoustic emission tests were conducted on 20%,40%,60%,80%,dry and saturated muddy sandstone by using a creep impact loading system to investigate the mechanical properties and acoustic emission characteristics of soft rocks with different water contents under dynamic disturbance.The mechanical properties and acoustic emission characteristics of muddy sandstones at different water contents were analysed.Results of experimental studies show that water is a key factor in the mechanical properties of rocks,softening them,increasing their porosity,reducing their brittleness and increasing their plasticity.Under uniaxial compression,the macroscopic damage characteristics of the muddy sandstone change from mono-bevel shear damage and‘X’type conjugate bevel shear damage to a roadway bottom-drum type damage as the water content increases.Dynamic perturbation has a strengthening effect on the mechanical properties of samples with 60%and less water content,and a weakening effect on samples with 80%and more water content,but the weakening effect is not obvious.Macroscopic damage characteristics of dry samples remain unchanged,water samples from shear damage and tensile–shear composite damage gradually transformed into cleavage damage,until saturation transformation monoclinic shear damage.The evolution of acoustic emission energy and event number is mainly divided into four stages:loading stage(Ⅰ),dynamic loading stage(Ⅱ),yield failure stage(Ⅲ),and post-peak stage(Ⅳ),the acoustic emission characteristics of the stages were different for different water contents.The characteristic value of acoustic emission key point frequency gradually decreases,and the damage degree of the specimen increases,corresponding to low water content—high main frequency—low damage and high water content—low main frequency—high damage. 展开更多
关键词 Dynamic disturbance Soft rock Cyclic loading Acoustic emission Water content
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Probabilistic analysis of tunnel face seismic stability in layered rock masses using Polynomial Chaos Kriging metamodel 被引量:2
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作者 Jianhong Man Tingting Zhang +1 位作者 Hongwei Huang Daniel Dias 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第7期2678-2693,共16页
Face stability is an essential issue in tunnel design and construction.Layered rock masses are typical and ubiquitous;uncertainties in rock properties always exist.In view of this,a comprehensive method,which combines... Face stability is an essential issue in tunnel design and construction.Layered rock masses are typical and ubiquitous;uncertainties in rock properties always exist.In view of this,a comprehensive method,which combines the Upper bound Limit analysis of Tunnel face stability,the Polynomial Chaos Kriging,the Monte-Carlo Simulation and Analysis of Covariance method(ULT-PCK-MA),is proposed to investigate the seismic stability of tunnel faces.A two-dimensional analytical model of ULT is developed to evaluate the virtual support force based on the upper bound limit analysis.An efficient probabilistic analysis method PCK-MA based on the adaptive Polynomial Chaos Kriging metamodel is then implemented to investigate the parameter uncertainty effects.Ten input parameters,including geological strength indices,uniaxial compressive strengths and constants for three rock formations,and the horizontal seismic coefficients,are treated as random variables.The effects of these parameter uncertainties on the failure probability and sensitivity indices are discussed.In addition,the effects of weak layer position,the middle layer thickness and quality,the tunnel diameter,the parameters correlation,and the seismic loadings are investigated,respectively.The results show that the layer distributions significantly influence the tunnel face probabilistic stability,particularly when the weak rock is present in the bottom layer.The efficiency of the proposed ULT-PCK-MA is validated,which is expected to facilitate the engineering design and construction. 展开更多
关键词 Tunnel face stability Layered rock masses Polynomial Chaos Kriging(PCK) Sensitivity index Seismic loadings
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Quantitative effect of kerogen type on the hydrocarbon generation potential of Paleogene lacustrine source rocks,Liaohe Western Depression,China 被引量:1
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作者 Sha-Sha Hui Xiong-Qi Pang +7 位作者 Fu-Jie Jiang Chen-Xi Wang Shu-Xing Mei Tao Hu Hong Pang Min Li Xiao-Long Zhou Kan-Yuan Shi 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期14-30,共17页
Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organ... Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organic carbon(TOC)content,various kerogen types,and a wide range of thermal maturity.Consequently,their hydrocarbon generation potential and resource estimation can be misinterpreted.In this study,geochemical tests,numerical analysis,hydrocarbon generation kinetics,and basin modeling were integrated to investigate the differential effects of kerogen types on the hydrocarbon generation potential of lacustrine source rocks.Optimized hydrocarbon generation and expulsion(HGE)models of different kerogen types were established quantitatively upon abundant Rock-Eval/TOC/vitrinite reflectance(R_(o))datasets.Three sets of good-excellent source rocks deposited in the fourth(Es4),third(Es3),and first(Es1)members of Paleogene Shahejie Formation,are predominantly types I-II_(1),II_(1)-II_(2),and II-III,respectively.The activation energy of types I-II_(2)kerogen is concentrated(180-230 kcal/mol),whereas that of type III kerogen is widely distributed(150-280 kcal/mol).The original hydrocarbon generation potentials of types I,II_(1),II_(2),and III kerogens are 790,510,270,and 85 mg/g TOC,respectively.The Ro values of the hydrocarbon generation threshold for type I-III source rocks gradually increase from 0.42%to 0.74%,and Ro values of the hydrocarbon expulsion threshold increase from 0.49%to 0.87%.Types I and II_(1)source rocks are characterized by earlier hydrocarbon generation,more rapid hydrocarbon expulsion,and narrower hydrocarbon generation windows than types II_(2)and III source rocks.The kerogen types also affect the HGE history and resource potential.Three types(conventional,tight,and shale oil/gas)and three levels(realistic,expected,and prospective)of hydrocarbon resources of different members in the Liaohe Western Depression are evaluated.Findings suggest that the Es3 member has considerable conventional and unconventional hydrocarbon resources.This study can quantitatively characterize the hydrocarbon generation potential of source rocks with different kerogen types,and facilitate a quick and accurate assessment of hydrocarbon resources,providing strategies for future oil and gas exploration. 展开更多
关键词 Kerogen type Hydrocarbon generation potential Lacustrine source rocks Liaohe western depression
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Multiscale modeling of gas-induced fracturing in anisotropic clayey rocks 被引量:1
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作者 Jianxiong Yang Jianfeng Liu +2 位作者 Zhengyuan Qin Xuhai Tang Houquan Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2091-2110,共20页
In the context of repositories for nuclear waste,understanding the behavior of gas migration through clayey rocks with inherent anisotropy is crucial for assessing the safety of geological disposal facilities.The prim... In the context of repositories for nuclear waste,understanding the behavior of gas migration through clayey rocks with inherent anisotropy is crucial for assessing the safety of geological disposal facilities.The primary mechanism for gas breakthrough is the opening of micro-fractures due to high gas pressure.This occurs at gas pressures lower than the combined strength of the rock and its minimum principal stress under external loading conditions.To investigate the mechanism of microscale mode-I ruptures,it is essential to incorporate a multiscale approach that includes subcritical microcracks in the modeling framework.In this contribution,we derive the model from microstructures that contain periodically distributed microcracks within a porous material.The damage evolution law is coupled with the macroscopic poroelastic system by employing the asymptotic homogenization method and considering the inherent hydro-mechanical(HM)anisotropy at the microscale.The resulting permeability change induced by fracture opening is implicitly integrated into the gas flow equation.Verification examples are presented to validate the developed model step by step.An analysis of local macroscopic response is undertaken to underscore the influence of factors such as strain rate,initial damage,and applied stress,on the gas migration process.Numerical examples of direct tension tests are used to demonstrate the model’s efficacy in describing localized failure characteristics.Finally,the simulation results for preferential gas flow reveal the robustness of the two-scale model in explicitly depicting gas-induced fracturing in anisotropic clayey rocks.The model successfully captures the common behaviors observed in laboratory experiments,such as a sudden drop in gas injection pressure,rapid build-up of downstream gas pressure,and steady-state gas flow following gas breakthrough. 展开更多
关键词 Deep geological repositories Mode-I microcracks Time-dependent damage Fracturing process Anisotropic rock
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