Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that ...Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial pathogen.The expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)accumulation.Interestingly,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS generation.Collectively,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.展开更多
In this study,photovoltage technique is applied in the development of a monolithically integrated sensor for redox(reduction–oxidation)potential and pH measurement.The sensor employs the electrolyte–insulator–semic...In this study,photovoltage technique is applied in the development of a monolithically integrated sensor for redox(reduction–oxidation)potential and pH measurement.The sensor employs the electrolyte–insulator–semiconductor structure,with deposition of a layer of gold metal on partial surface of insulator silicon dioxide.Silicon dioxide and gold layer on a single chip form two distinct sensing sites,by sharing the same measuring system,the detection of redox potential and pH variation can be realized.In this work,the sensor characteristics is tested,and the sensitivity for redox potential and pH measurement is53.8 mV/log([Fe(II)]/[Fe(III)])and 44.3 mV/pH respectively.To demonstrate the validity of the sensor in extracellular detection,neonatal rat kidney cells are cultured on the sensor surface and then packaged in a flow chamber,thus the acidification rate of metabolites and the redox potential variation in extracellular microenvironment can be continuously monitored.Experimental results indicate increasing acidification and reducing potentials under physiological conditions.The synthesis parameters have potentials in detail revelation of cell metabolism.展开更多
Metallomacromolecules are frequently encountered in redox proteins including metal-tanned hide collagen and play crucial roles involving supramolecular properties in biological electron-transfer processes.They are als...Metallomacromolecules are frequently encountered in redox proteins including metal-tanned hide collagen and play crucial roles involving supramolecular properties in biological electron-transfer processes.They are also currently found in non-natural families,such as:metallopolymers,metallodendrimers and metallodendronic polymers.This mini-review discusses the supramolecular redox functions of such nanomaterials developed in our research group.Electron-transfer processes are first examined in mono-,bis-and hexa-nuclear ferrocenes and other electron-reservoir organoiron systems showing the influence of supramolecular and reorganization aspects on their mechanism.Then applications of electron-transfer processes using these same organoiron redox systems in metallomacromolecules and their supramolecular functions are discussed including redox recognition/sensing,catalysis templates,electrocatalysis,redox catalysis,molecular machines,electrochromes,drug delivery device and nanobatteries.展开更多
The detection of peroxide explosives（PEs） has attracted considerable attention all over the world in global security owing to their simple preparation,poor chemical stability and easy decomposition.In recent years,g...The detection of peroxide explosives（PEs） has attracted considerable attention all over the world in global security owing to their simple preparation,poor chemical stability and easy decomposition.In recent years,great efforts have been devoted to developing organic fluorescence sensors for detecting the PEs because of their fast response,high sensitivity and high selectivity.In this short review,we firstly discuss the sensing mechanisms for fluorescence based the PEs detection.Next,we reviewed recent progress of PE probes in the nearly 5 years and the design strategies of the material structures to enhance the sensitivity or selectivity,such as conjugated polymers and assembled nanoparticles.展开更多
基金supported by grants from the"BioGreen21 Agri-Tech Inovation Program(project no.PJ015824 to S.Y.L.and PJ0159992021 to M.G.K.)",Rural Development Administration(RDA),South Koreaby the Basic Science Research Program through the National Research Foundation(NRF)of South Korea funded by the Ministry of Education(NRF-2018R1A6A3A11049525 to H.B.C.).
文摘Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial pathogen.The expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)accumulation.Interestingly,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS generation.Collectively,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.
基金supported by the National Nat-ural Science Foundation of China(81027003)the National Basic Research Program of China(2009CB320303)the National Public Welfare Project of China(201305010)
文摘In this study,photovoltage technique is applied in the development of a monolithically integrated sensor for redox(reduction–oxidation)potential and pH measurement.The sensor employs the electrolyte–insulator–semiconductor structure,with deposition of a layer of gold metal on partial surface of insulator silicon dioxide.Silicon dioxide and gold layer on a single chip form two distinct sensing sites,by sharing the same measuring system,the detection of redox potential and pH variation can be realized.In this work,the sensor characteristics is tested,and the sensitivity for redox potential and pH measurement is53.8 mV/log([Fe(II)]/[Fe(III)])and 44.3 mV/pH respectively.To demonstrate the validity of the sensor in extracellular detection,neonatal rat kidney cells are cultured on the sensor surface and then packaged in a flow chamber,thus the acidification rate of metabolites and the redox potential variation in extracellular microenvironment can be continuously monitored.Experimental results indicate increasing acidification and reducing potentials under physiological conditions.The synthesis parameters have potentials in detail revelation of cell metabolism.
基金The University of Bordeauxthe Centre National de la Recherche Scientifique(CNRS)+2 种基金the Institut Universitaire de France(IUF)the FP7 European Program“Nanosolution”L’Oreal Research Center.
文摘Metallomacromolecules are frequently encountered in redox proteins including metal-tanned hide collagen and play crucial roles involving supramolecular properties in biological electron-transfer processes.They are also currently found in non-natural families,such as:metallopolymers,metallodendrimers and metallodendronic polymers.This mini-review discusses the supramolecular redox functions of such nanomaterials developed in our research group.Electron-transfer processes are first examined in mono-,bis-and hexa-nuclear ferrocenes and other electron-reservoir organoiron systems showing the influence of supramolecular and reorganization aspects on their mechanism.Then applications of electron-transfer processes using these same organoiron redox systems in metallomacromolecules and their supramolecular functions are discussed including redox recognition/sensing,catalysis templates,electrocatalysis,redox catalysis,molecular machines,electrochromes,drug delivery device and nanobatteries.
基金supported by NSFC(Nos.61325001,21273267,61321492,and 51473182)
文摘The detection of peroxide explosives（PEs） has attracted considerable attention all over the world in global security owing to their simple preparation,poor chemical stability and easy decomposition.In recent years,great efforts have been devoted to developing organic fluorescence sensors for detecting the PEs because of their fast response,high sensitivity and high selectivity.In this short review,we firstly discuss the sensing mechanisms for fluorescence based the PEs detection.Next,we reviewed recent progress of PE probes in the nearly 5 years and the design strategies of the material structures to enhance the sensitivity or selectivity,such as conjugated polymers and assembled nanoparticles.
