目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织...目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织中的定位进行研究;人精子免疫荧光对REEP6在精子顶体反应前后的定位进行研究。结果:REEP6在人睾丸及精子中均有表达,在睾丸组织中REEP6定位于各级生精细胞的细胞膜,在精子中则定位于核后区附近,并且在顶体反应前后,REEP6在人精子的定位及表达量不发生改变。结论:REEP6在人睾丸和精子中均有表达,其表达定位具有细胞特异性和区域特异性,提示其可能在人睾丸精子发生和(或)精卵融合过程中发挥作用。展开更多
A decline in the activities of oxidative phosphorylation(OXPHOS)complexes has been consistently reported in amyotrophic lateral sclerosis(ALS)patients and animal models of ALS,although the underlying molecular mechani...A decline in the activities of oxidative phosphorylation(OXPHOS)complexes has been consistently reported in amyotrophic lateral sclerosis(ALS)patients and animal models of ALS,although the underlying molecular mechanisms are still elusive.Here,we report that receptor expression enhancing protein 1(REEP1)acts as an important regulator of complex IV assembly,which is pivotal to preserving motor neurons in SOD1^(G93A) mice.We found the expression of REEP1 was greatly reduced in transgenic SOD1^(G93A) mice with ALS.Moreover,forced expression of REEP1 in the spinal cord extended the lifespan,decelerated symptom progression,and improved the motor performance of SOD1^(G93A) mice.The neuromuscular synaptic loss,gliosis,and even motor neuron loss in SOD1^(G93A) mice were alleviated by increased REEP1 through augmentation of mitochondrial function.Mechanistically,REEP1 associates with NDUFA4,and plays an important role in preserving the integrity of mitochondrial complex IV.Our findings offer insights into the pathogenic mechanism of REEP1 deficiency in neurodegenerative diseases and suggest a new therapeutic target for ALS.展开更多
Geogrids are used as reinforcement materials widely in geotechnical and civil engineering fields. In this paper, a series of comparative tests on creep behavior of specific geoOgrids are conducted in the laboratory un...Geogrids are used as reinforcement materials widely in geotechnical and civil engineering fields. In this paper, a series of comparative tests on creep behavior of specific geoOgrids are conducted in the laboratory under different combinations of loading levels and environmental temperature. Based on the test results,comparative analyses are made to study long-term behaviors of isochronous load-strain curves, creep curves and relaxation curves for the specified geogrids. Furthermore, a constitutive model based on theory of visco-elasticity and tests results is proposed for geogrids and a rational procedure is presented in detail for determining the relevant parameters of the proposed model. Finally, the effect of tests temperature on model parameter values is investigated.展开更多
文摘目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织中的定位进行研究;人精子免疫荧光对REEP6在精子顶体反应前后的定位进行研究。结果:REEP6在人睾丸及精子中均有表达,在睾丸组织中REEP6定位于各级生精细胞的细胞膜,在精子中则定位于核后区附近,并且在顶体反应前后,REEP6在人精子的定位及表达量不发生改变。结论:REEP6在人睾丸和精子中均有表达,其表达定位具有细胞特异性和区域特异性,提示其可能在人睾丸精子发生和(或)精卵融合过程中发挥作用。
基金supported by Shandong Key R&D Program Funding(2018GSF118037)the Shandong Natural Science Foundation(ZR2019JQ24).
文摘A decline in the activities of oxidative phosphorylation(OXPHOS)complexes has been consistently reported in amyotrophic lateral sclerosis(ALS)patients and animal models of ALS,although the underlying molecular mechanisms are still elusive.Here,we report that receptor expression enhancing protein 1(REEP1)acts as an important regulator of complex IV assembly,which is pivotal to preserving motor neurons in SOD1^(G93A) mice.We found the expression of REEP1 was greatly reduced in transgenic SOD1^(G93A) mice with ALS.Moreover,forced expression of REEP1 in the spinal cord extended the lifespan,decelerated symptom progression,and improved the motor performance of SOD1^(G93A) mice.The neuromuscular synaptic loss,gliosis,and even motor neuron loss in SOD1^(G93A) mice were alleviated by increased REEP1 through augmentation of mitochondrial function.Mechanistically,REEP1 associates with NDUFA4,and plays an important role in preserving the integrity of mitochondrial complex IV.Our findings offer insights into the pathogenic mechanism of REEP1 deficiency in neurodegenerative diseases and suggest a new therapeutic target for ALS.
文摘Geogrids are used as reinforcement materials widely in geotechnical and civil engineering fields. In this paper, a series of comparative tests on creep behavior of specific geoOgrids are conducted in the laboratory under different combinations of loading levels and environmental temperature. Based on the test results,comparative analyses are made to study long-term behaviors of isochronous load-strain curves, creep curves and relaxation curves for the specified geogrids. Furthermore, a constitutive model based on theory of visco-elasticity and tests results is proposed for geogrids and a rational procedure is presented in detail for determining the relevant parameters of the proposed model. Finally, the effect of tests temperature on model parameter values is investigated.
