As a highly sensitive and stable detector,refractive index detector is usually used for quantitative detection of substances such as polymer,sugar and organic acid. The research reviewed the application of HPLC-RID in...As a highly sensitive and stable detector,refractive index detector is usually used for quantitative detection of substances such as polymer,sugar and organic acid. The research reviewed the application of HPLC-RID in the fields of quantitative determination of medicine and food,in order to lay a foundation for wider use of RID.展开更多
A simple and direct method without a deriva- tion step for routine analysis of tobramycin has been developed. This method used reversed-phase ion-pair high performance liquid chromatography （HPLC） with a refractive ...A simple and direct method without a deriva- tion step for routine analysis of tobramycin has been developed. This method used reversed-phase ion-pair high performance liquid chromatography （HPLC） with a refractive index （RI） detector and a C18 column which is stable at pH above 1.00. The presence of 4.50mg.mL ] trifluoroacetic acid （TFA） in the mobile phase improved the protonation of tobramycin and the formation of ionpairs, and thus reduced its hydrophility. This unique separation-detection combination showed good linearity with correlation coefficients 0.9996 in the concentration range of 0.25-2.50mg.mL 1. The quantitation limit and detection limit were determined to be 0.23 mg.mL ~ and 0.071 mg. mL ~, respectively. Tobramycin was recovered in 98.00%, 98.84% and 99.64% for tobramycin solutions at concentrations of 2.25 mg.mL^-1, 1.50mg.mL 1 and 0.75 mg.mL ＇, respectively. The relative standard deviations for six spiked samples ranged from 0.20% to 2.40%, indicating a good reproducibility of this method.展开更多
目的建立高效液相色谱-示差折光检测器法(HPLC-RID)测定盐酸金刚烷胺片中盐酸金刚烷胺的含量。方法采用Waters Sun Fire C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(10:90),柱温30℃,进样量20μL,流速1.0 mL/min,检测器为R...目的建立高效液相色谱-示差折光检测器法(HPLC-RID)测定盐酸金刚烷胺片中盐酸金刚烷胺的含量。方法采用Waters Sun Fire C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(10:90),柱温30℃,进样量20μL,流速1.0 mL/min,检测器为RID,检测池温度30℃。结果盐酸金刚烷胺的线性范围为0.2513~2.5130mg/mL(r=0.9994),平均回收率为100.1%,RSD为0.9%(n=9)。结论该方法简便、快速、准确、重现性好,可用于盐酸金刚烷胺片的含量测定。展开更多
文摘As a highly sensitive and stable detector,refractive index detector is usually used for quantitative detection of substances such as polymer,sugar and organic acid. The research reviewed the application of HPLC-RID in the fields of quantitative determination of medicine and food,in order to lay a foundation for wider use of RID.
文摘A simple and direct method without a deriva- tion step for routine analysis of tobramycin has been developed. This method used reversed-phase ion-pair high performance liquid chromatography （HPLC） with a refractive index （RI） detector and a C18 column which is stable at pH above 1.00. The presence of 4.50mg.mL ] trifluoroacetic acid （TFA） in the mobile phase improved the protonation of tobramycin and the formation of ionpairs, and thus reduced its hydrophility. This unique separation-detection combination showed good linearity with correlation coefficients 0.9996 in the concentration range of 0.25-2.50mg.mL 1. The quantitation limit and detection limit were determined to be 0.23 mg.mL ~ and 0.071 mg. mL ~, respectively. Tobramycin was recovered in 98.00%, 98.84% and 99.64% for tobramycin solutions at concentrations of 2.25 mg.mL^-1, 1.50mg.mL 1 and 0.75 mg.mL ＇, respectively. The relative standard deviations for six spiked samples ranged from 0.20% to 2.40%, indicating a good reproducibility of this method.
文摘目的建立高效液相色谱-示差折光检测器法(HPLC-RID)测定盐酸金刚烷胺片中盐酸金刚烷胺的含量。方法采用Waters Sun Fire C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(10:90),柱温30℃,进样量20μL,流速1.0 mL/min,检测器为RID,检测池温度30℃。结果盐酸金刚烷胺的线性范围为0.2513~2.5130mg/mL(r=0.9994),平均回收率为100.1%,RSD为0.9%(n=9)。结论该方法简便、快速、准确、重现性好,可用于盐酸金刚烷胺片的含量测定。
