The plant regeneration frequencies of ealli fromplant tissue and cell culture,especially that of thecalli from rice tissue culture and rice anther cul-ture,and that of the foreign-DNA-transfor-mation-derived rice call...The plant regeneration frequencies of ealli fromplant tissue and cell culture,especially that of thecalli from rice tissue culture and rice anther cul-ture,and that of the foreign-DNA-transfor-mation-derived rice calli is very low(usually 10-15%).It is therefor very important to improve theplant regeneration frequency of rice calli.A1-展开更多
In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature ...In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3% sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month's in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same展开更多
We attempted to introduce apomictic gene(s)into rice via somatic hybridization by usingapomictic Panicum maximum Jacq.as thedonor of apomictic gene(s).Protoplasts of rice derived from suspen-sion cells were inactivate...We attempted to introduce apomictic gene(s)into rice via somatic hybridization by usingapomictic Panicum maximum Jacq.as thedonor of apomictic gene(s).Protoplasts of rice derived from suspen-sion cells were inactivated with indoacetamide(IOA)and protoplasts of Panicum maximum展开更多
We studied the effect of agar concentration inmedia on callus induction rate and green plant-let regeneration frequency in rice.Materialswere Fgeneration of indica/indica or indica/japonica,which were 96E76(Hei’e/Zha...We studied the effect of agar concentration inmedia on callus induction rate and green plant-let regeneration frequency in rice.Materialswere Fgeneration of indica/indica or indica/japonica,which were 96E76(Hei’e/Zhaiye- qing 8),96E80[(IR 24/Guanglu'ai 4//Zhongnan’ai)/Yifengzao],96E86(Zhong- munong 9/Zhaiyeqing 8).The induction mediaused was M8+2mg/L 2,4-D,and agar con-centrations were 0.6%,0.8%,and 1.0%,respectively.Regeneration media was MS+2mg/L KT+0.5mg/L IAA+0.5mg/LNAA,and agar concentrations were 0.6% and1.0%.Results indicated that the calli induc-展开更多
In the recent decade,plant regeneration fromprotoplast has been obtained through embryo-genic cell suspension cultures of rice.Howev-er,not only the establishment of embryogeniccell suspension cultures of rice was dif...In the recent decade,plant regeneration fromprotoplast has been obtained through embryo-genic cell suspension cultures of rice.Howev-er,not only the establishment of embryogeniccell suspension cultures of rice was difficult,but also the protoplasts became less and lessregenerable and the genetic change was gradu-ally accumulated during the prolonged culture.Since 1976(Deka.),extensive efforts have展开更多
Enrichment of copper to the culture mediumcould enhance the plant regeneration from cal-lus of indica rice variety Qiugui’ai 11. Westudied the effect of copper on plant regenera-tion of other rice varieties.Calli of ...Enrichment of copper to the culture mediumcould enhance the plant regeneration from cal-lus of indica rice variety Qiugui’ai 11. Westudied the effect of copper on plant regenera-tion of other rice varieties.Calli of 14 indica and 2 japonica varietieswere induced from disinfected mature embryoson an agar-gelled medium containing Nbasal展开更多
Rice selection 02428 and T984(Oryzasativa L.ssp.japonica)were germplasmresources with wide compatibility.Mature embryos of rice cultured on Lin-smaier and Skoog medium with 2.5 mg/l2,4-D,1.0 mg/l thiamine-HCL,3%(W/V)s...Rice selection 02428 and T984(Oryzasativa L.ssp.japonica)were germplasmresources with wide compatibility.Mature embryos of rice cultured on Lin-smaier and Skoog medium with 2.5 mg/l2,4-D,1.0 mg/l thiamine-HCL,3%(W/V)sucrose and 0.7%(W/V)agar,pH 5.8(LS2.5)were used for callus initiation.Cultures were展开更多
文摘The plant regeneration frequencies of ealli fromplant tissue and cell culture,especially that of thecalli from rice tissue culture and rice anther cul-ture,and that of the foreign-DNA-transfor-mation-derived rice calli is very low(usually 10-15%).It is therefor very important to improve theplant regeneration frequency of rice calli.A1-
文摘In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3% sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month's in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same
文摘We attempted to introduce apomictic gene(s)into rice via somatic hybridization by usingapomictic Panicum maximum Jacq.as thedonor of apomictic gene(s).Protoplasts of rice derived from suspen-sion cells were inactivated with indoacetamide(IOA)and protoplasts of Panicum maximum
文摘We studied the effect of agar concentration inmedia on callus induction rate and green plant-let regeneration frequency in rice.Materialswere Fgeneration of indica/indica or indica/japonica,which were 96E76(Hei’e/Zhaiye- qing 8),96E80[(IR 24/Guanglu'ai 4//Zhongnan’ai)/Yifengzao],96E86(Zhong- munong 9/Zhaiyeqing 8).The induction mediaused was M8+2mg/L 2,4-D,and agar con-centrations were 0.6%,0.8%,and 1.0%,respectively.Regeneration media was MS+2mg/L KT+0.5mg/L IAA+0.5mg/LNAA,and agar concentrations were 0.6% and1.0%.Results indicated that the calli induc-
文摘In the recent decade,plant regeneration fromprotoplast has been obtained through embryo-genic cell suspension cultures of rice.Howev-er,not only the establishment of embryogeniccell suspension cultures of rice was difficult,but also the protoplasts became less and lessregenerable and the genetic change was gradu-ally accumulated during the prolonged culture.Since 1976(Deka.),extensive efforts have
文摘Enrichment of copper to the culture mediumcould enhance the plant regeneration from cal-lus of indica rice variety Qiugui’ai 11. Westudied the effect of copper on plant regenera-tion of other rice varieties.Calli of 14 indica and 2 japonica varietieswere induced from disinfected mature embryoson an agar-gelled medium containing Nbasal
文摘Rice selection 02428 and T984(Oryzasativa L.ssp.japonica)were germplasmresources with wide compatibility.Mature embryos of rice cultured on Lin-smaier and Skoog medium with 2.5 mg/l2,4-D,1.0 mg/l thiamine-HCL,3%(W/V)sucrose and 0.7%(W/V)agar,pH 5.8(LS2.5)were used for callus initiation.Cultures were