Considering the continuous advancement in the field of imaging sensor, a host of other new issues have emerged. A major problem is how to find focus areas more accurately for multi-focus image fusion. The multi-focus ...Considering the continuous advancement in the field of imaging sensor, a host of other new issues have emerged. A major problem is how to find focus areas more accurately for multi-focus image fusion. The multi-focus image fusion extracts the focused information from the source images to construct a global in-focus image which includes more information than any of the source images. In this paper, a novel multi-focus image fusion based on Laplacian operator and region optimization is proposed. The evaluation of image saliency based on Laplacian operator can easily distinguish the focus region and out of focus region. And the decision map obtained by Laplacian operator processing has less the residual information than other methods. For getting precise decision map, focus area and edge optimization based on regional connectivity and edge detection have been taken. Finally, the original images are fused through the decision map. Experimental results indicate that the proposed algorithm outperforms the other series of algorithms in terms of both subjective and objective evaluations.展开更多
AIM:To examine the imprinted Dlk1-Dio3 locus in pluripotent embryonic stem(ES)cell/fibroblast hybrid cells.METHODS:Gtl2,Rian,and Mirg mRNA expression in mouse pluripotent ES cell/fibroblast hybrid cells was examined b...AIM:To examine the imprinted Dlk1-Dio3 locus in pluripotent embryonic stem(ES)cell/fibroblast hybrid cells.METHODS:Gtl2,Rian,and Mirg mRNA expression in mouse pluripotent ES cell/fibroblast hybrid cells was examined by real-time reverse transcription-polymerase chain reaction.Pyrosequencing and bisulfate sequencing were used to determine the DNA methylation level of the Dlk1-Dio3 locus imprinting control region. RESULTS:The selected hybrid clones had a near-tetraploid karyotype and were highly pluripotent judging from their capacity to generate chimeric embryos and adult chimeras.Our data clearly demonstrate that Gtl2,Rian,and Mirg,which are imprinted genes within the Dlk1-Dio3 locus,are active in all examined ES cell/fibroblast hybrid clones.In spite of interclonal variability,the expression of the imprinted genes is comparable to that of ES cells and fibroblasts.Quantitative analysis of the DNA methylation status of the intergenic differentially methylated region(IG DMR)within the Dlk1-Dio3 locus by pyrosequencing and bisulfite sequencing clearly showed that the DNA methylation status of the imprinted region in the tested hybrid clones was comparable to that of both ES cells and fibroblasts.CONCLUSION:Reprogramming process in a hybrid cell system is achieved without marked alteration of the imprinted Dlk1-Dio3 locus.展开更多
This paper describes a method to decompose multi-scale information from different source medical image using wavelet transformation. The data fusion between CT image and MRI image is implemented based on the coefficie...This paper describes a method to decompose multi-scale information from different source medical image using wavelet transformation. The data fusion between CT image and MRI image is implemented based on the coefficients fusion rule which included choice of regional variance and weighted average wavelet information. The result indicates that this method is better than WMF, LEF and RVF on fusion results, details and target distortion.展开更多
为了克服基于单一视差合成的光场图像编码方法在遮挡区域无法恢复纹理细节的问题,提出一种基于多特征融合和几何感知网络的光场图像编码方法,以进一步提升遮挡场景下光场图像的压缩性能.首先,对密集光场稀疏采样,使用通用视频编码器(ver...为了克服基于单一视差合成的光场图像编码方法在遮挡区域无法恢复纹理细节的问题,提出一种基于多特征融合和几何感知网络的光场图像编码方法,以进一步提升遮挡场景下光场图像的压缩性能.首先,对密集光场稀疏采样,使用通用视频编码器(versatile video coding,VVC)对稀疏光场进行压缩;然后,在解码端使用2个关键分支模块,即视差估计模块和空间角度联合卷积模块,以获取光场图像全局的几何信息,确保在密集纹理和遮挡区域能够更充分地恢复特征;最后,为了挖掘2个分支融合特征的结构信息,构建了双向视图的堆栈结构,并运用几何感知的细化网络以重建高质量的密集光场.实验结果表明,与已有国际上流行的光场图像编码方法相比,所提出的方法具有显著优势.展开更多
文摘Considering the continuous advancement in the field of imaging sensor, a host of other new issues have emerged. A major problem is how to find focus areas more accurately for multi-focus image fusion. The multi-focus image fusion extracts the focused information from the source images to construct a global in-focus image which includes more information than any of the source images. In this paper, a novel multi-focus image fusion based on Laplacian operator and region optimization is proposed. The evaluation of image saliency based on Laplacian operator can easily distinguish the focus region and out of focus region. And the decision map obtained by Laplacian operator processing has less the residual information than other methods. For getting precise decision map, focus area and edge optimization based on regional connectivity and edge detection have been taken. Finally, the original images are fused through the decision map. Experimental results indicate that the proposed algorithm outperforms the other series of algorithms in terms of both subjective and objective evaluations.
基金Supported by Grant from the Russian Academy of Sciences,Siberian Branch N48
文摘AIM:To examine the imprinted Dlk1-Dio3 locus in pluripotent embryonic stem(ES)cell/fibroblast hybrid cells.METHODS:Gtl2,Rian,and Mirg mRNA expression in mouse pluripotent ES cell/fibroblast hybrid cells was examined by real-time reverse transcription-polymerase chain reaction.Pyrosequencing and bisulfate sequencing were used to determine the DNA methylation level of the Dlk1-Dio3 locus imprinting control region. RESULTS:The selected hybrid clones had a near-tetraploid karyotype and were highly pluripotent judging from their capacity to generate chimeric embryos and adult chimeras.Our data clearly demonstrate that Gtl2,Rian,and Mirg,which are imprinted genes within the Dlk1-Dio3 locus,are active in all examined ES cell/fibroblast hybrid clones.In spite of interclonal variability,the expression of the imprinted genes is comparable to that of ES cells and fibroblasts.Quantitative analysis of the DNA methylation status of the intergenic differentially methylated region(IG DMR)within the Dlk1-Dio3 locus by pyrosequencing and bisulfite sequencing clearly showed that the DNA methylation status of the imprinted region in the tested hybrid clones was comparable to that of both ES cells and fibroblasts.CONCLUSION:Reprogramming process in a hybrid cell system is achieved without marked alteration of the imprinted Dlk1-Dio3 locus.
文摘This paper describes a method to decompose multi-scale information from different source medical image using wavelet transformation. The data fusion between CT image and MRI image is implemented based on the coefficients fusion rule which included choice of regional variance and weighted average wavelet information. The result indicates that this method is better than WMF, LEF and RVF on fusion results, details and target distortion.
文摘为了克服基于单一视差合成的光场图像编码方法在遮挡区域无法恢复纹理细节的问题,提出一种基于多特征融合和几何感知网络的光场图像编码方法,以进一步提升遮挡场景下光场图像的压缩性能.首先,对密集光场稀疏采样,使用通用视频编码器(versatile video coding,VVC)对稀疏光场进行压缩;然后,在解码端使用2个关键分支模块,即视差估计模块和空间角度联合卷积模块,以获取光场图像全局的几何信息,确保在密集纹理和遮挡区域能够更充分地恢复特征;最后,为了挖掘2个分支融合特征的结构信息,构建了双向视图的堆栈结构,并运用几何感知的细化网络以重建高质量的密集光场.实验结果表明,与已有国际上流行的光场图像编码方法相比,所提出的方法具有显著优势.
