Comparative analysis reveals remarkable homology between the sequences of both psbA gene nucleotidesand the inferred amino acids of sorghum, a C4 plant, and those of rice, a C3 plant. The 5'-noncoding region of so...Comparative analysis reveals remarkable homology between the sequences of both psbA gene nucleotidesand the inferred amino acids of sorghum, a C4 plant, and those of rice, a C3 plant. The 5'-noncoding region of sorghum psbA gene contains the conservative promoter elements, ' - 35' element and ' - 10' element, like the prokaryote and the promoter element, TATA box, like the eukaryote. As compared with that of the rice, an extra sequence of 7 bp is found in the leader sequence of the mRNA in the former. Using an in vitro system, it has been demonstrated that protein factor exists in sorghum chloroplast protein extract which specifically binds to the 5'-noncoding region of psbA gene. Measurement of the expression of luciferase shows a 2-5 time greater reaction of the expression plasmids pALqs which contain leader region of sorghum psbA gene than that of the expression plasmids pALqr which contain leader region of rice psbA gene in E. coli.展开更多
根据已报道的hsa(人血清白蛋白基因)启动子序列设计了一对引物,以猪的基因组DNA为模板,采用PCR扩增出一条256 bp DNA片段,用Primer Premier5.0和Promoter Scan软件进行分析。结果表明,此序列为psa基因5′-端上游包括启动子在内的调控区...根据已报道的hsa(人血清白蛋白基因)启动子序列设计了一对引物,以猪的基因组DNA为模板,采用PCR扩增出一条256 bp DNA片段,用Primer Premier5.0和Promoter Scan软件进行分析。结果表明,此序列为psa基因5′-端上游包括启动子在内的调控区,该区域含有一般启动子典型的CAAT-box、TATA-box等基本顺式作用元件。另外,还含有CTF/NF-1、CTF、APF、HNF-1、CP1等转录因子的结合位点。展开更多
基金Project supported by the Chinese National "863" and "973" Projects.
文摘Comparative analysis reveals remarkable homology between the sequences of both psbA gene nucleotidesand the inferred amino acids of sorghum, a C4 plant, and those of rice, a C3 plant. The 5'-noncoding region of sorghum psbA gene contains the conservative promoter elements, ' - 35' element and ' - 10' element, like the prokaryote and the promoter element, TATA box, like the eukaryote. As compared with that of the rice, an extra sequence of 7 bp is found in the leader sequence of the mRNA in the former. Using an in vitro system, it has been demonstrated that protein factor exists in sorghum chloroplast protein extract which specifically binds to the 5'-noncoding region of psbA gene. Measurement of the expression of luciferase shows a 2-5 time greater reaction of the expression plasmids pALqs which contain leader region of sorghum psbA gene than that of the expression plasmids pALqr which contain leader region of rice psbA gene in E. coli.
文摘根据已报道的hsa(人血清白蛋白基因)启动子序列设计了一对引物,以猪的基因组DNA为模板,采用PCR扩增出一条256 bp DNA片段,用Primer Premier5.0和Promoter Scan软件进行分析。结果表明,此序列为psa基因5′-端上游包括启动子在内的调控区,该区域含有一般启动子典型的CAAT-box、TATA-box等基本顺式作用元件。另外,还含有CTF/NF-1、CTF、APF、HNF-1、CP1等转录因子的结合位点。
