[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of th...[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of the structural protein VP3 were predicted by software analysis,and the region displaying a large portion of antigenic epitopes was amplified by PCR. The target VP3 DNA fragment was inserted into pET-30 a-VP3 vector, was transformed into Escherichia coli BL21 competent cells for protein expression and animal test. The SPF chickens were immunized with the recombinant protein and the antisera were collected for neutralization test by using a goose embryo fibroblast. [Result] The recombinant plasmid was constructed, and the target region of VP3 protein was expressed efficiently in a soluble form. The neutralizing titers of antisera could reach up to-2.608. [Conclusion] The target region displaying a large portion of antigenic epitopes of the structural protein VP3 could be expressed efficiently in soluble form, and the expressed protein could induce neutralizing antibodies in SFP chicken.展开更多
Flushing time of the Yangtze estuary by discharge is one of the importantfactors responsible for the transport of pollutants from various sources located along the Yangtzeestuary: Therefore, an objective of the presen...Flushing time of the Yangtze estuary by discharge is one of the importantfactors responsible for the transport of pollutants from various sources located along the Yangtzeestuary: Therefore, an objective of the present stud-y, which analysis flushing time in the case ofdifferent discharge is very helpful to evaluate the water environmental of the Yangtze estuary.Using a dissolved conservative material as a tracer in the water, a three-dimensionadvection-diffusion water exchange numerical model was used to study the flushing time by dischargeand the discharge dominated region of the Yangtze estuary. The initial tracer concentration is setto 0.0 in the numerical domain of the Yangtze estuary, and the concentration value is set to 1. 0 onthe inflow boundary. The tracer flux normal to the solid boundary is set to 0. 0. The flushing timeand the out limit of discharge dominated region can be calculated in terms of the tracerconcentration. Estuarine, Coastal and Ocean Model (ECOM) is used as the hydro-dynamic model. Theresult shows that the flushing time is approximately in inverse proportion to the discharge at theupper stream. The out limit is farther from the upper inflow boundary as discharge increases. Theout limit at the north branch is different from that of the south branch because the discharge intothe north branch is much less than that into the south branch. The data is qualitative similar tothe observed data, which show the three-dimensional advection-diffusion equation can be used toestimate the flushing time and the discharge dominated region of the Yangtze estuary.展开更多
基金Supported by Youth Fund Project of Natural Science Foundation of Shandong Province(ZR2014CQ009)Science and Technology Development Program of Binzhou City(2013GG0304)
文摘[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of the structural protein VP3 were predicted by software analysis,and the region displaying a large portion of antigenic epitopes was amplified by PCR. The target VP3 DNA fragment was inserted into pET-30 a-VP3 vector, was transformed into Escherichia coli BL21 competent cells for protein expression and animal test. The SPF chickens were immunized with the recombinant protein and the antisera were collected for neutralization test by using a goose embryo fibroblast. [Result] The recombinant plasmid was constructed, and the target region of VP3 protein was expressed efficiently in a soluble form. The neutralizing titers of antisera could reach up to-2.608. [Conclusion] The target region displaying a large portion of antigenic epitopes of the structural protein VP3 could be expressed efficiently in soluble form, and the expressed protein could induce neutralizing antibodies in SFP chicken.
文摘Flushing time of the Yangtze estuary by discharge is one of the importantfactors responsible for the transport of pollutants from various sources located along the Yangtzeestuary: Therefore, an objective of the present stud-y, which analysis flushing time in the case ofdifferent discharge is very helpful to evaluate the water environmental of the Yangtze estuary.Using a dissolved conservative material as a tracer in the water, a three-dimensionadvection-diffusion water exchange numerical model was used to study the flushing time by dischargeand the discharge dominated region of the Yangtze estuary. The initial tracer concentration is setto 0.0 in the numerical domain of the Yangtze estuary, and the concentration value is set to 1. 0 onthe inflow boundary. The tracer flux normal to the solid boundary is set to 0. 0. The flushing timeand the out limit of discharge dominated region can be calculated in terms of the tracerconcentration. Estuarine, Coastal and Ocean Model (ECOM) is used as the hydro-dynamic model. Theresult shows that the flushing time is approximately in inverse proportion to the discharge at theupper stream. The out limit is farther from the upper inflow boundary as discharge increases. Theout limit at the north branch is different from that of the south branch because the discharge intothe north branch is much less than that into the south branch. The data is qualitative similar tothe observed data, which show the three-dimensional advection-diffusion equation can be used toestimate the flushing time and the discharge dominated region of the Yangtze estuary.
