An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.

Functional connectivity of paired default mode network subregions in patients with eye trauma

AIM:To study functional brain abnormalities in patients with eye trauma(ET)and to discuss the pathophysiological mechanisms of ET.METHODS:Totally 31 ET patients and 31 healthy controls(HCs)were recruited.The age,gender,and educational background characteristics of the two groups were similar.After functional magnetic resonance imaging(fMRI)scanning,the subjects’spontaneous brain activity was evaluated with the functional connectivity(FC)method.Receiver operating characteristic(ROC)curve analysis was used to classify the data.Pearson’s correlation analysis was used to explore the relationship between FC values in specific brain regions and clinical behaviors in patients with ET.RESULTS:Significantly increased FC between several regions was identified including the medial prefrontal cortex(MPFC)and left hippocampus formations(HF),the MPFC and left inferior parietal lobule(IPL),the left IPL and left medial temporal lobe(MTL),the left IPL and right MTL,and the right IPL and left MTL.No decreased region-to-region connectivity was detected in default mode network(DMN)sub-regions in patients with ET.Compared with HCs,ET patients exhibited significantly increased FC between several paired DMN regions,as follows:posterior cingulate cortex(PCC)and right HF(HF.R,t=2.196,P=0.032),right inferior parietal cortices(IPC.R)and left MTL(MTL.L,t=2.243,P=0.029),and right MTL(MTL.R)and HF.R(t=2.236,P=0.029).CONCLUSION:FC values in multiple brain regions of ET patients are abnormal,suggesting that these brain regions in ET patients may be dysfunctional,which may help to reveal the pathophysiological mechanisms of ET.

Artificial ground freezing of underground mines in cold regions using thermosyphons with air insulation

Current practice of underground artificial ground freezing(AGF)typically involves huge refrigeration systems of large economic and environmental costs.In this study,a novel AGF technique is proposed deploying available cold wind in cold regions.This is achieved by a static heat transfer device called thermosyphon equipped with an air insulation layer.A refrigeration unit can be optionally integrated to meet additional cooling requirements.The introduction of air insulation isolates the thermosyphon from ground zones where freezing is not needed,resulting in:(1)steering the cooling resources(cold wind or refrigeration)towards zones of interest;and(2)minimizing refrigeration load.This design is demonstrated using well-validated mathematical models from our previous work based on two-phase enthalpy method of the ground coupled with a thermal resistance network for the thermosyphon.Two Canadian mines are considered:the Cigar Lake Mine and the Giant Mine.The results show that our proposed design can speed the freezing time by 30%at the Giant Mine and by two months at the Cigar Lake Mine.Further,a cooling load of 2.4 GWh can be saved at the Cigar Lake Mine.Overall,this study provides mining practitioners with sustainable solutions of underground AGF.

Interaction between diet and genetics in patients with inflammatory bowel disease

In this editorial,we comment on the article by Marangoni et al,published in the recent issue of the World Journal of Gastroenterology 2023;29:5618-5629,about“Diet as an epigenetic factor in inflammatory bowel disease”.The authors emphasized the role of diet,especially the interaction with genetics,in promoting the inflam-matory process in inflammatory bowel disease(IBD)patients,focusing on DNA methylation,histone modifications,and the influence of microRNAs.In this editorial,we explore the interaction between genetics,gut microbiota,and diet,in an only way.Furthermore,we provided dietary recommendations for patients with IBD.The Western diet,characterized by a low fiber content and deficiency the micronutrients,impacts short-chain fatty acids production and may be related to the pathogenesis of IBD.On the other hand,the consumption of the Mediter-ranean diet and dietary fibers are associated with reduced risk of IBD flares,particularly in Crohn’s disease(CD)patients.According to the dietary guidance from the International Organization for the Study of Inflammatory Bowel Diseases(IOIBD),the regular consumption of fruits and vegetables while reducing the consumption of saturated,trans,dairy fat,additives,processed foods rich in maltodextrins,and artificial sweeteners containing sucralose or saccharine is recommended to CD patients.For patients with ulcerative colitis,the IOIBD recommends the increased intake of natural sources of omega-3 fatty acids and follows the same restrictive recommendations aimed at CD patients,with the possible inclusion of red meats.In conclusion,IBD is a complex and hetero-geneous disease,and future studies are needed to elucidate the influence of epigenetics on diet and microbiota in IBD patients.

A phylogenetic approach identifies patterns of beta diversity and floristic subregions of the Qinghai-Tibet Plateau

Patterns of taxonomic and phylogenetic beta diversity and their relationships with environmental correlates can help reveal the origin and evolutionary history of regional biota.The Qinghai-Tibet Plateau(QTP)harbors an exceptionally diverse flora,however,a phylogenetic perspective has rarely been used to investigate its beta diversity and floristic regions.In this study,we used a phylogenetic approach to identify patterns of beta diversity and quantitatively delimit floristic regions on the Qinghai-Tibet Plateau.We also examined the relationships between multifaceted beta diversity,geographical distance,and climatic difference,and evaluated the relative importance of various factors(i.e.,climate,topography and history)in shaping patterns of beta diversity.Sørensen dissimilarity indices indicated that patterns of species turnover among sites dominated the QTP.We also found that patterns of both taxonomic and phylogenetic beta diversity were significantly related to geographical distance and climatic difference.The environmental factors that contributed most to these patterns of beta diversity include annual precipitation,mean annual temperature,climatic gradients and climatic instability.Hierarchical dendrograms of dissimilarity and non-metric multidimensional scaling ordination based on phylogenetic beta diversity data identified ten floristic subregions in the QTP.Our results suggest that the contemporary environment and historical climate changes have filtered species composition among sites and eventually determined beta diversity patterns of plants in the QTP.

Factors affecting land dissection density in geomorphological regions of China

Land dissection density(LDD)provides morphological evidence regarding prior intense soil erosion and quantifies the distribution of land dissections.A comprehensive understanding of the potential factors influencing the spatial pattern and value of the LDD is vital in geological disasters,soil erosion,and other related domains.Land dissection phenomena in China affects large areas with different morphological,pedological,and climatic characteristics.Prior studies have focused on the potential factors influencing the LDD at a watershed scale.However,these results are insufficient to reflect the status quo of dissection development and its primary influencing factors on a national scale.LDD’s spatial patterns and the dominant factors at a regional scale in millions of square kilometers remain to be ascertained.This study used the geomorphon-based method and the geographical detector model to quantify the spatial pattern of LDD over China and identify the dominant factors affecting this pattern in China’s six first-order geomorphological regions(GR1~GR6).The results yield the following findings:(1)LDD in China ranges from 0~4.55 km/km^(2),which is larger in central and eastern regions than in other regions of China;(2)dominant factors and their dominant risk subcategories vary with each geomorphological region’s primary internal and external forces;(3)the influence of natural factors is more significant on the large regional scale in millions of square kilometers compared to anthropogenic factors;relief degree of land surface(RDLS)is dominant in GR1,GR2,and GR5;the slope is dominant in GR6,soil type is dominant in GR3 and GR4,and lithology plays a critical role in the dominant interactions of GR3,GR4,and GR6;(4)the interactions between factors on LDD’s spatial pattern have a more significant effect than individual factors.

Research on the Digital Economy's Impact on China's Economic Growth:Based on the Variations in Urbanization Levels Across China's Eastern,Central,and Western Regions and Sectoral Heterogeneity

This paper uses inter-provincial panel data from 2011 to 2017,a linear regression model,and a threshold model to conduct empirical analyses of the impact of the digital economy on China's overall economic growth and the three main sectors of industry.The paper then investigates the impact and effects the digital economy has had on the economic growth of the three main sectors of industry in China's eastern,central,and western regions.Finally,the paper investigates the most significant differences among the various regions and the threshold effects of urbanization levels on the relationship between the digital economy and economic growth.The findings indicate a significantly positive correlation between the digital economy and regional economic growth.Moreover,geographical factors notably influence this correlation.The digital economy exerts a positive effect on all sectors of industry.It may not substantially impact industrial development in regions with highly developed infrastructure.Regarding the other regions,the digital economy exhibits varying degrees of impact due to the differences in the specific indicators.The conclusion drawn by the threshold model is that the magnitude of the threshold effect correlates with geographic factors.No threshold effect was observed in the eastern region,while the threshold effect occurred in the central region when the urbanization levels for the provinces were below 0.6645.Similarly,the threshold effect was noted in the western region when the urbanization level was below 0.3931.Considering all of this,the study also offers policy recommendations that will help balance the regional development of digital economies,accelerate the digital transformation of traditional industries,enhance digital infrastructure construction,refine the formulation and implementation of data policy,and establish relevant incentive mechanisms.

Strategies and Economic Benefit Analysis for Productive Landscape of Rape Flower Sea in Cold Regions

The cold regions are located in high latitudes and cold climates.The local excellent ornamental plant resources are relatively scarce.The plant species that are suitable for both ornamental and productive benefits of landscape of flower sea construction are also even fewer.Therefore,it is imperative to introduce and screen the plant resources suitable for cold regions to create the landscape of flower sea.The rape,an oilseed crop,was used as a research object in order to create a productive flower landscape with both ornamental and economic values in cold regions.Four rape flower varieties,Qingza No.5,7,9,and 11,were introduced from Qinghai Hufeng Agricultural Science and Technology Group Co.,Ltd.They were planted in the experimental practice base of Northeast Agricultural University in three batches.Development characteristics and seed yield of rape flowers on different sowing dates were studied.The fuzzy probability method was used to comprehensively evaluate the varieties.The results showed that the rape flowers grew well in Harbin City during the experimental sowing period,which could form a good landscape of flower sea and had a considerable rapeseed yield.It could be widely used in cold urban and rural areas,such as Harbin City.In view of the experimental results,the strategies of creating a productive landscape of rape flower sea were proposed and the economic benefits were analyzed.It could change the status quo of a uniform landscape of flower sea in cold regions,help the development of rural tourism,and promote local economic income.

A Theory of Bio-Quantum Genetics

The physical mechanism of heredity or inheritance of genes is a quantum mechanical and/or quantum computational process. A theory of bio-quantum genetics is established in this paper. Principle of Bio-quantum Genetics is suggested. I propose and define the soft-genes of genetics controlling the processes of heredity or inheritance of genes. This research deals with the quantum mechanisms of Mendel plant heredity and family inheritance as examples of bio-quantum genetics, deepening our understanding of heredity or inheritance. I believe that more contributions will be made to promote researches of bio-quantum genetics or quantum biology at large.

The Practice and Exploration of Applying EBM to Bilingual Teaching of Medical Genetics at OSBCM

In the process of teaching medical genetics of undergraduate clinical medicine, the practice and exploration of applying EBM to the bilingual teaching of OSBCM medical genetics are carried out. Using CBL and PBL as the carrier can make up for the shortcomings of a single teaching mode, synthesize the advantages of multiple teaching modes. It starts from integrating the basic theoretical knowledge of medicine and clinical practice knowledge, improving students’ bilingual level of medical genetics, cultivating students’ literature retrieval ability, and promoting early clinical, multi-clinical and repeated clinical consciousness for medical students. Therefore, it is more conducive to cultivate students’ ability to learn independently, accurately analyze and solve problems, improve medical students’ clinical thinking ability and scientific research awareness, improve medical students’ ability of international communication, and lay a solid foundation for improving medical students’ future post competence, innovative spirit and lifelong learning ability.

An Assessment of Saltwater Intrusion in Coastal Regions of Lagos, Nigeria

This paper explains various factors that contribute to saltwater intrusion, including overexploitation of freshwater resources and climate change as well as the different techniques essential for effective saltwater intrusion management. The impact of saltwater intrusion along coastal regions and its impact on the environment, hydrogeology and groundwater contamination. It suggests potential solutions to mitigate the impact of saltwater intrusion, including effective water management and techniques for managing SWI. The application of A.I (assessment index) serves as a guideline to correctly identify wells with SWI ranging from no intrusion, slight intrusion and strong intrusion. The challenges of saltwater intrusion in Lagos and the salinization of wells were investigated using the hydro-chemical parameters. The study identifies four wells (“AA”, “CMS”, “OBA” and “VIL”) as having high electric conductivities, indicating saline water intrusion, while other wells (“EBM”, “IKJ, and “IKO”) with lower electric conductivities, indicate little or no salt-water intrusion, and “AJ” well shows slight intrusion. The elevation of the wells also played a vital role in the SWI across coastal regions of Lagos. The study recommends continuous monitoring of coastal wells to help sustain and reduce saline intrusion. The findings of the study are important for policymakers, researchers, and practitioners who are interested in addressing the challenges of saltwater intrusion along coastal regions. We assessed the SWI across the eight (8) wells using the Assessment Index to identify wells with SWI. Wells in “CMS” and “VIL” has strong intrusions. A proposed classification system based on specific ion ratios categorizes water quality from good (+) to highly (-) contaminated (refer to Table 4). These findings underscore the need for attention and effective management strategies to address groundwater unsuitability for various purposes.

Innovative Technologies for Large-Scale Water Production in Arid Regions: Strategies for Sustainable Development

Water scarcity in arid regions poses significant challenges to sustainable development and human well-being. This article explores both existing and innovative technologies and methods to produce large amounts of water to address these challenges effectively. Key approaches include atmospheric water generation, advanced desalination techniques, innovative water collection methods such as fog nets and dew harvesting, geothermal water extraction, and water recycling and reuse. Each method is evaluated for its feasibility with existing technology, potential time of implementation, required investments, and specific challenges. By leveraging these technologies and combining them into a multifaceted water management strategy, it is possible to enhance water security, support agricultural and industrial activities, and improve living conditions in arid regions. Collaborative efforts between governments, private sector entities, and research institutions are crucial to advancing these technologies and ensuring their sustainable implementation. The article provides a comprehensive overview of the current state of these technologies, their potential for large-scale application, and recommendations for future research and development.

Spatio-temporal variation of depth to groundwater level and its driving factors in arid and semi-arid regions of India

Climate change and increasing anthropogenic activities,such as over-exploitation of groundwater,are exerting unavoidable stress on groundwater resources.This study investigated the spatio-temporal variation of depth to groundwater level(DGWL)and the impacts of climatic(precipitation,maximum temperature,and minimum temperature)and anthropogenic(gross district product(GDP),population,and net irrigated area(NIA))variables on DGWL during 1994-2020.The study considered DGWL in 113 observation wells and piezometers located in arid western plains(Barmer and Jodhpur districts)and semi-arid eastern plains(Jaipur,Ajmer,Dausa,and Tonk districts)of Rajasthan State,India.Statistical methods were employed to examine the annual and seasonal patterns of DGWL,and the generalized additive model(GAM)was used to determine the impacts of climatic and anthropogenic variables on DGWL.During 1994-2020,except for Barmer District,where the mean annual DGWL was almost constant(around 26.50 m),all other districts exhibited increase in DGWL,with Ajmer District experiencing the most increase.The results also revealed that 36 observation wells and piezometers showed a statistically significant annual increasing trend in DGWL and 34 observation wells and piezometers exhibited a statistically significant decreasing trend in DGWL.Similarly,32 observation wells and piezometers showed an statistically significant increasing trend and 37 observation wells and piezometers showed a statistically significant decreasing trend in winter;33 observation wells and piezometers indicated a statistically significant increasing trend and 34 had a statistically significant decreasing trend in post-monsoon;35 observation wells and piezometers exhibited a statistically significant increasing trend and 32 observation wells and piezometers showed a statistically significant decreasing trend in pre-monsoon;and 36 observation wells and piezometers reflected a statistically significant increasing trend and 30 observation wells and piezometers reflected a statistically significant decreasing trend in monsoon.Interestingly,most of the observation wells and piezometers with increasing trends of DGWL were located in Dausa and Jaipur districts.Furthermore,the GAM analysis revealed that climatic variables,such as precipitation,significantly affected DGWL in Barmer District,and DGWL in all other districts was influenced by anthropogenic variables,including GDP,NIA,and population.As a result,stringent regulations should be implemented to curb excessive groundwater extraction,manage agricultural water demand,initiate proactive aquifer recharge programs,and strengthen sustainable management in these water-scarce regions.

Circulating metabolites difference in type 2 diabetes patients from regions:a cross-sectional study

Background:Type 2 diabetes(T2D)has already become a global pandemic.As its simple,rapid,economical,and relatively non-invasive,metabolic markers have become a method for T2D diagnosis.However,region,race,and diet all affect the metabolism of the body.The purpose of current study is to explore the differences of metabolites in T2D patients from regions.Methods:We recruited 103 T2D patients in two clinical centers,including 52 T2D patients from Beijing(T2D_(B))and 51 T2D patients from Kaifeng(T2D_(K)).The serum samples from T2D patients were analyzed using high-resolution mass spectrometer.After screened using univariate and multivariate analysis,the differential metabolites were identified.Moreover,to reveal biological information,we performed pathway analysis with the differential metabolites.Results:Thirty-six differential metabolites were identified,including 16 metabolites were higher concentrations while 20 metabolites were lower concentrations in the serum of T2D_(B) patients than T2D_(K) patients.There were higher serum concentrations of L-phenylalanine,4-methyl-2-oxovaleric acid,L-carnitine,decanoylcarnitine,9-decenoylcarnitine and sphinganine in T2D_(B) patients,in which decanoylcarnitine in T2D_(B) patients was up to 35-fold higher than T2D_(K) patients.While there were lower concentrations of L-valine,L-isoleucine,arachidonic acid,oleic acid,16-hydroxyhexadecanoic acid,lysophosphatidylcholine(18:0)and 1-Phenylethylamine in T2D_(B) patients,in which 1-phenylethylamine in T2D_(B) patients was decreased to 0.45-fold lower than T2D_(K) patients.The reason for the differences might be that phosphatidylethanolamine biosynthesis,phosphatidylcholine biosynthesis,valine,leucine and isoleucine degradation,and beta-oxidation of very long chain fatty acids were different in T2D_(B) patients and in T2D_(K) patients.Conclusion:Metabolites from different pathways are independently related to regions,providing valuable insight and potential for the diagnosis and treatment of T2D.

Population genetics of marmosets in Asian primate research centers and loci associated with epileptic risk revealed by whole-genome sequencing

The common marmoset(Callithrix jacchus)has emerged as a valuable nonhuman primate model in biomedical research with the recent release of high-quality reference genome assemblies.Epileptic marmosets have been independently reported in two Asian primate research centers.Nevertheless,the population genetics within these primate centers and the specific genetic variants associated with epilepsy in marmosets have not yet been elucidated.Here,we characterized the genetic relationships and risk variants for epilepsy in 41 samples from two epileptic marmoset pedigrees using whole-genome sequencing.We identified 14558184 single nucleotide polymorphisms(SNPs)from the 41 samples and found higher chimerism levels in blood samples than in fingernail samples.Genetic analysis showed fourth-degree of relatedness among marmosets at the primate centers.In addition,SNP and copy number variation(CNV)analyses suggested that the WW domain-containing oxidoreductase(WWOX)and Tyrosine-protein phosphatase nonreceptor type 21(PTPN21)genes may be associated with epilepsy in marmosets.Notably,KCTD18-like gene deletion was more common in epileptic marmosets than control marmosets.This study provides valuable population genomic resources for marmosets in two Asian primate centers.Genetic analyses identified a reasonable breeding strategy for genetic diversity maintenance in the two centers,while the case-control study revealed potential risk genes/variants associated with epilepsy in marmosets.

Chemical composition of walnuts from three regions in China

To reveal the characteristic chemicals of walnuts from different origins, we analyzed fatty acid composition,tocopherols, phytosterols and total phenolic content (TPC) of walnuts from three main producing regions inChina. The results showed that walnuts were rich in polyunsaturated fatty acids, and the ratio of ω-6 to ω-3 fattyacids was close to the recommendation of Chinese Nutrition Society. Moreover, walnuts contain high contents oftocopherols (331.20–414.71 mg/kg), phytosterols (97.17–110.35 mg/100 g) and phenols (38.51–48.08 mg GAE/kg). Significant chemical differences exist among walnuts from three production regions. The highest content ofpolyunsaturated fatty acids was found in walnuts from the northern China, the highest content of tocopherols inwalnuts from southwest China, and the highest contents of phytosterol and TPC in walnuts from northwest China.However, there was no significant difference in the tocopherol, phytosterol and TPC content of walnuts betweenthe Northern China and Northwest China. The above results provide important references for manufacturers andconsumers to select suitable walnut scientifically and reasonably.

Plastic-film-side seeding,as an alternative to traditional film mulching,improves yield stability and income in maize production in semi-arid regions

Planting under plastic-film mulches is widely used in spring maize production in arid-cold regions for water conservation and warming the soil.To ameliorate the associated issues such as plastic-film residues and additional labor during the“seedling release”in spring maize production,we have developed a plastic-film-side seeding(PSS)technology with the supporting machinery.In the semi-arid regions of Northwest China,a 7-year trial demonstrated that PSS increased plant number per hectare by 6547 and maize yield by 1686 kg ha–1compared with the traditional method of seeding under plastic-film mulch(PM).Two-year experiments were conducted in two semi-arid regions to further understand the effects of PSS on three important aspects of production:(i)the moisture and temperature of soil,(ii)maize development,yield output,and water use efficiency(WUE),and(iii)the revenue and plastic-film residuals in comparison with that of flat planting(CK)and PM.Continuous monitoring of the soil status demonstrated that,compared with CK,the PSS treatment significantly increased the temperature and moisture of the 0–20 cm soil in the seeding row at the early stage of maize development,and it also promoted grain yield(at 884–1089 kg ha^(–1))and WUE,achieving a similar effect as the PM treatment.Economically,the labor inputs of PSS were equal to CK,whereas the PM cost an additional 960 CNY ha–1in labor for releasing the seedlings from below the film.Overall,the PSS system increased profits by 5.83%(547 CNY ha^(–1)yr^(–1))and 8.16%(748 CNY ha^(–1)yr^(–1))compared with CK and PM,respectively.Environmentally,PSS achieved a residual film recovery rate of nearly 100%and eliminated 96 to 130 kg ha^(–1)of residual plastic-film in PM in 3–5 years of maize production.Collectively,these results show that PSS is an eco-friendly technique for improving yield stability and incomes for the sustainable production of maize in semi-arid regions.

Histone H3K27me3 methylation regulates the expression of secreted proteins distributed at fast-evolving regions through transcriptional repression of transposable elements

The fine-tuned expression dynamics of the effector genes are pivotal for the transition from vegetative growth to host colonization of pathogenic filamentous fungi.However,mechanisms underlying the dynamic regulation of these genes remain largely unknown.Here,through comparative transcriptome and chromatin immunoprecipitation sequencing(ChIP-seq)analyses of the methyltransferase PoKmt6 in rice blast fungus Pyricularia oryzae(syn.Magnaporthe oryzae),we found that PoKmt6-mediated H3K27me3 deposition was enriched mainly at fast-evolving regions and contributed to the silencing of a subset of secreted proteins(SP)and transposable element(TE)families during the vegetative growth of P.oryzae.Intriguingly,we observed that a group of SP genes,which were depleted of H3K27me3 modification,could also be silenced via the H3K27me3-mediated repression of the nearby TEs.In conclusion,our results indicate that H3K27me3 modification mediated by PoKmt6 regulates the expression of some SP genes in fast-evolving regions through the suppression of nearby TEs.

Dissecting the key genomic regions underlying high yield potential in common wheat variety‘Kenong 9204’

The foundation parents play key roles in the genetic improvement of both yield potential and end-use quality in wheat.Characterizing the genetic basis that underlies certain beneficial traits in the foundation parents will provide theoretical reference for molecular breeding by a design approach.‘Kenong 9204’(KN9204)is a candidate foundation parent characterized by ideotype,high yield potential,and particularly high nitrogen fertilizer utilization.To better understand the genetic basis of its high yield potential,high throughput whole-genome re-sequencing(10×)was performed on KN9204,its parental lines and its derivatives.A high-resolution genetic composition map of KN9204 was constructed,which showed the parental origin of the favorable genomic segments based on the identification of excellent yield-related quantitative trait loci(QTL)from a bi-parental mapping population.Xiaoyan 693(XY693),a wheat–Thinopyrum ponticum partial amphidiploid,contributed a great deal to the high yield potential of KN9204,and three major stable QTLs from XY693 were fine mapped.The transmissibility of key genomic segments from KN9204 to its derivatives were delineated,indicating that haplotype blocks containing beneficial gene combinations were conserved along with directional selection by breeders.Evidence for selection sweeps in the breeding programs was identified.This study provides a theoretical reference for the breeding of high-yield wheat varieties by a molecular design approach.

Molecular phylogenetics and population demographic history of Amphioctopus fangsiao,inferred from mitochondrial and microsatellite DNA markers

Amphioctopus fangsiao(Cephalopoda:Octopodidae)is an important commercial species in the coastal waters of China.In recent years,however,the resource of A.fangsiao have declined because of habitat destruction and overfishing.To analyze the genetic variations of A.fangsiao caused by the fluctuation of resources,the population genetic structure of nine sampling locations collected from the Bohai Sea to the South China Sea were investigated,using mtDNA COI fragments and microsatellite DNA.The results of F-statistics,AMOVA,STRUCTURE and PCA analyses showed three phylogeographic clades(Clades A,B and C),revealing limited genetic exchange between north and south populations.These clades diverged in 2.23(Clades A and B)and 3.67(Clades A,B and C)million years ago,during the dramatic environmental fluctuations,such as sea level and temperature changes,have exerted great influence on the survival distribution pattern of global organisms.Our results for low genetic connectivity among A.fangsiao populations provide insights into the development of management strategies,that is,to manage this species as separate management unit.