AIM: To investigate whether DNA-dependent activator of interferon-regulatory factors (DAI) inhibits hepatitis B virus (HBV) replication and what the mechanism is. METHODS: After the human hepatoma cell line Huh7 was c...AIM: To investigate whether DNA-dependent activator of interferon-regulatory factors (DAI) inhibits hepatitis B virus (HBV) replication and what the mechanism is. METHODS: After the human hepatoma cell line Huh7 was cotransfected with DAI and HBV expressing plasmid, viral protein (HBV surface antigen and HBV e antigen) secretion was detected by enzyme-linked immunosorbent assay, and HBV RNA was analyzed by real-time polymerase chain reaction and Northern blotting, and viral DNA replicative intermediates were examined by Southern blotting. Interferon regulatory factor 3 (IRF3) phosphorylation and nuclear translocation were analyzed via Western blotting and immunofluorescence staining respectively. Nuclear factor-B (NF- B) activity induced by DAI was detected by immunofluorescence staining of P65 and dual luciferase reporter assay. Transwell co-culture experiment was performed in order to investigate whether the antiviral effects of DAI were dependent on the secreted cytokines. RESULTS: Viral protein secretion was significantly reduced by 57% (P < 0.05), and the level of total HBV RNA was reduced by 67% (P < 0.05). The viral core particle-associated DNA was also dramatically down-regulated in DAI-expressing Huh7 cells. Analysis of involved signaling pathways revealed that activation of NF-B signaling was essential for DAI to elicit antiviral response in Huh7 cells. When the NF-B signaling pathway was blocked by a NF-B signaling suppressor (I B -SR), the anti-HBV activity of DAI was remarkably abrogated. The inhibitory effect of DAI was independent of IRF3 signaling and secreted cytokines. CONCLUSION: This study demonstrates that DAI can inhibit HBV replication and the inhibitory effect is associated with activation of NF-B but independent of IRF3 and secreted cytokines.展开更多
In this paper, we consider the reconstruction of the wave field in a bounded domain. By choosing a special family of functions, the Cauchy problem can be transformed into a Fourier moment problem. This problem is ill-...In this paper, we consider the reconstruction of the wave field in a bounded domain. By choosing a special family of functions, the Cauchy problem can be transformed into a Fourier moment problem. This problem is ill-posed. We propose a regularization method for obtaining an approximate solution to the wave field on the unspecified boundary. We also give the convergence analysis and error estimate of the numerical algorithm. Finally, we present some numerical examples to show the effectiveness of this method.展开更多
Good afternoon!It's a great pleasure to share my views on Carthaginian civilisation with friends from both Tunisia and China.The Carthaginian civilisation is a compound of the Phoenician civilisation and ancient T...Good afternoon!It's a great pleasure to share my views on Carthaginian civilisation with friends from both Tunisia and China.The Carthaginian civilisation is a compound of the Phoenician civilisation and ancient Tunisian civilisation,and its interaction with the contemporaneous Roman civilisation gave birth to the splendid Mediterranean civilisation.展开更多
基金Supported by Grants of The Chinese State Basic Research, No.2009CB522504National Mega Projects for Infectious Diseases, No. 2008ZX10203
文摘AIM: To investigate whether DNA-dependent activator of interferon-regulatory factors (DAI) inhibits hepatitis B virus (HBV) replication and what the mechanism is. METHODS: After the human hepatoma cell line Huh7 was cotransfected with DAI and HBV expressing plasmid, viral protein (HBV surface antigen and HBV e antigen) secretion was detected by enzyme-linked immunosorbent assay, and HBV RNA was analyzed by real-time polymerase chain reaction and Northern blotting, and viral DNA replicative intermediates were examined by Southern blotting. Interferon regulatory factor 3 (IRF3) phosphorylation and nuclear translocation were analyzed via Western blotting and immunofluorescence staining respectively. Nuclear factor-B (NF- B) activity induced by DAI was detected by immunofluorescence staining of P65 and dual luciferase reporter assay. Transwell co-culture experiment was performed in order to investigate whether the antiviral effects of DAI were dependent on the secreted cytokines. RESULTS: Viral protein secretion was significantly reduced by 57% (P < 0.05), and the level of total HBV RNA was reduced by 67% (P < 0.05). The viral core particle-associated DNA was also dramatically down-regulated in DAI-expressing Huh7 cells. Analysis of involved signaling pathways revealed that activation of NF-B signaling was essential for DAI to elicit antiviral response in Huh7 cells. When the NF-B signaling pathway was blocked by a NF-B signaling suppressor (I B -SR), the anti-HBV activity of DAI was remarkably abrogated. The inhibitory effect of DAI was independent of IRF3 signaling and secreted cytokines. CONCLUSION: This study demonstrates that DAI can inhibit HBV replication and the inhibitory effect is associated with activation of NF-B but independent of IRF3 and secreted cytokines.
文摘In this paper, we consider the reconstruction of the wave field in a bounded domain. By choosing a special family of functions, the Cauchy problem can be transformed into a Fourier moment problem. This problem is ill-posed. We propose a regularization method for obtaining an approximate solution to the wave field on the unspecified boundary. We also give the convergence analysis and error estimate of the numerical algorithm. Finally, we present some numerical examples to show the effectiveness of this method.
文摘Good afternoon!It's a great pleasure to share my views on Carthaginian civilisation with friends from both Tunisia and China.The Carthaginian civilisation is a compound of the Phoenician civilisation and ancient Tunisian civilisation,and its interaction with the contemporaneous Roman civilisation gave birth to the splendid Mediterranean civilisation.
