Repair of necrosis and defects of penile skin with autologous free skin flap

We described a 27-year-old case of avulsion and traumatic degloving of penile with extensive penis skin necrosis. Under general anesthesia, donor skin was partially resected from lower limbs according to defect area of penile skin. Then shear the shape of graft was sheared, sutured to hostage skin defect and enswathed with tension. The postoperative appearance and function of the penis were satisfactory. It is suggest the homologous free skin flap from lower limbs is suitable for penile skin repair and beneficial to patient resulting in satisfactory erection and shape.

Skin-Adhesive Tape versus Interrupted Suture in Episiotomy Skin Repair: Randomized Control Trial

Introduction: The ideal method for perineal repair should be quick, easy to perform and preferably, with minimal pain. Aim: To compare skin adhesive tape and interrupted?absorbable subcuticular suture for episiotomy repair after birth as regard postoperative pain, skin closure time and wound infection. Design: Prospective double-blinded randomized controlled trial. Methods: Three-hundred qualified patients were equally distributed between two groups. Group 1 underwent skin repair with skin adhesive tape, while group 2 underwent the currently traditional method for perineal repair by interrupted suture. Pain was evaluated at 2, 4, 6 and 12 hours after birth then daily until one week using Wong-Baker faces pain rating scale with verbal expression for pain intensity as primary outcome. Skin closure time and wound infection were also evaluated as secondary outcomes. Results: Statistically significant difference in pain was?found at 4 and 6 hours, and 3,?4,?5 and 7 days after perineal repair in favor of the adhesive tape group (p = 0.04 and 0.02 respectively) (p = 0.002, 0.002, 0.003 and 0.001 respectively). No statistically significant difference was found in skin closure time between both groups, and no cases of wound infection occurred in both groups (p = 0.3). Conclusion: Skin adhesive tape may be superior to skin suturing in decreasing pain resulting from perineal repair after birth;however, further studies are needed to assess long-term effects, calculate costs and accurately measure patients’ satisfaction, which were not addressed in this study.

One-stitch anastomosis through the skin with bicanalicular intubation:a modified approach for repair of bicanalicular laceration

AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular laceration.METHODS:The clinical data of 15 consecutive patients with both superior and inferior canalicular laceration in one eye who underwent surgical repair using one-stitch anastomosis through the skin and bicanalicular stent were retrospective studied.All the operations were performed under surgical microscope,5-0 silk sutures were used and were with bicanalicular silicone tube(diameter was 8mm) intubation,for one lacerated canaliculi one-stitch anastomosis through the skin.The stents were left in place for 3 months postoperatively and then removed.The follow-up period was 3-36 months(average 14 months). RESULTS:In 15 patients,13 patients were cured entirely,1 patient was meliorated,1 patient with no effects.All patients had got good recovery of eyelid laceration with no traumatic deformity in eyelid and canthus.Complication was seen in one case,for not followed the doctor’s guidance to come back to hospital to had the suture removed on the 7thday after operation,when he came at the 15thday,the inferior canalicular wall and eyelid skin were corroded by the suture caused 2mm wound,and the inside silicone tube was exposed,a promptly repair with 10-0 nylon suture was done,the wound healed in a week.There were no early tube protrusions and punctal slits in the patients.CONCLUSION:One-stitch anastomosis through the skin with bicanalicular silicone tube intubation is a good method in repair of bicanalicular laceration in one eye,the cut ends can be anastomosed directly,and with excellent cosmetic results,it is acceptable for the patients.For there is no suture remained in the wound permanently,so there is no suture-related granuloma which may cause obstruction or stenosis of canaliculi.It is simple,economical,effective and safe.

Clinical Application of Direct Skin Grafting Repair in Popliteal Scar Contracture after Burn in Children

Objective To observe the clinical application of direct skin grafting in repairing popliteal scar contracture after burn in children.Methods From April 2017 to January 2019,30 children with popliteal scar contracture after burn were selected as the research objects.The scar status,knee joint function and quality of life of the patients before and after the operation were compared by direct skin grafting after medium thickness skin removal.Results 30 patients were treated with skin grafting to repair popliteal scar contracture,27 patients healed well,and the survival rate of skin grafting reached 90.00%.The scores of postoperative scar and knee joint function scale(WOMAC)were lower than those before the operation,and the scores of concise health status scale(SF-36)were higher than those before the operation,with statistical significance(P<0.05).Conclusion Scar contracture of popliteal fossa after burn in children can be repaired by direct skin grafting in the skin extraction area after medium thickness skin cutting,which can effectively improve scar condition,restore knee joint function and significantly improve quality of life.

Repair of facial skin defect with a skin flap of SMAS pedicle

To report a method of repair facial skin defects with a skin flap of SMAS pedicle.Methods According to the size of defect of skin,design a skin flap with SMAS pedicle for repair of defect.Results The method has been successfully applied for skin defects of eyelid and lip in 14 cases with satisfied results.The area of the largest flap was 5 cm×3 cm.Conclusion Repairing facial defects such as eyelid skin defect or lip skin defect with skin flap of SMAS pedicle is a very good method.The flap has a good blood supporting and satisfactory color and flexibility.5 refs,6 figs.

改良腓动脉穿支螺旋桨皮瓣在足踝部皮肤软组织缺损中的修复效果

目的:探讨足踝部皮肤软组织缺损患者采用改良腓动脉穿支螺旋桨皮瓣修复的效果。方法:选择2019年7月—2022年12月贵州省人民医院整形烧伤外科收治的100例足踝部皮肤软组织缺损行皮瓣修复的患者。按照随机数表法分为对照组和观察组,各50例,对照组予以腓动脉穿支皮瓣修复,观察组予以改良腓动脉穿支螺旋桨皮瓣修复。观察两组皮瓣情况(皮瓣一期成活率、增生性瘢痕、色素沉着发生率)、皮瓣感觉功能、踝关节活动度(踝关节跖屈、背伸角度)及踝关节功能[美国足踝外科协会(AOFAS)踝与后足评分系统评分]。结果:观察组皮瓣一期成活率为94.00%,高于对照组的80.00%,差异有统计学意义(P<0.05);两组术后增生性瘢痕、色素沉着发生率比较,差异无统计学意义(P>0.05)。观察组皮瓣感觉恢复优良率为88.00%,高于对照组的72.00%,差异有统计学意义(P<0.05)。术后6个月,两组踝关节跖屈、背伸角度高于术前,且观察组高于对照组,差异有统计学意义(P<0.05)。术后6个月,两组疼痛、力线及功能维度评分高于术前,且观察组高于对照组,差异有统计学意义(P<0.05)。结论:改良腓动脉穿支螺旋桨皮瓣在足踝部皮肤软组织缺损修复中血运可靠,能改善踝关节功能,促进皮瓣感觉功能恢复,提高皮瓣一期成活率。

改良V-Y推进皮瓣对面部皮肤肿瘤术后缺损患者的修复效果研究

目的探究改良V-Y推进皮瓣对面部皮肤肿瘤术后缺损患者的修复效果。方法前瞻性选取河南宏力医院2020年2月至2023年2月收治的40例面部皮肤肿瘤切除术后皮肤缺损的患者,均接受改良V-Y推进皮瓣修复。结果40例患者皮片和皮瓣全部成活,其中创面为Ⅰ期愈合38例,并发皮瓣边缘部分坏死1例,经局部修剪换药后愈合;切口感染1例,经短期换药后愈合。随访1~12个月,失访1例,于术后1年复发3例,局部肿瘤复发1例,腮腺区淋巴结转移2例,均再次行根治性切除术治疗后行补充放疗,其余患者面部外形、功能均恢复良好36例,伤口美观满意度为94.44%(34/36)。结论改良V-Y推进皮瓣拓宽了V-Y推进皮瓣修复缺损的适应证,降低了术后一期继发性缺损及并发症发生率,皮瓣存活率高,且美观度好。

耳后沟带蒂皮瓣修复联合循证护理对难治性耳前瘘管反复感染患儿疗效及美学效果观察

目的探讨耳后沟带蒂皮瓣修复联合循证护理在难治性耳前瘘管反复感染患儿瘘管修复的可行性及优势。方法随机将2020年2月~2022年1月收治的83例反复感染耳前瘘管患儿分为观察组(42例)和对照组(41例),两组患儿均实施耳前瘘管切除术,对照组耳前瘘管切除后直接周围皮肤减张拉拢缝合;观察组耳前瘘管切除后游离耳后沟皮瓣带蒂翻转至耳前缝合,术后配合循证护理。观察两组术后并发症、切口愈合程度、疗效及美学效果。结果两组患儿均顺利完成手术,对照组术后并发症发生率为19.51%,明显高于观察组2.38%,差异有统计学意义(χ2=4.651,P=0.031)。观察组切口Ⅰ期愈合率高于对照组,Ⅱ期愈合率低于对照组,差异均具有统计学意义(P<0.05)。随访1年,两组患儿耳前瘘管均痊愈,治愈率均为100%,但观察组平均愈合时间为(11.63±3.24)d,明显较对照组(19.25±4.81)d短,差异具有统计学意义(t=8.483,P=0.000)。观察组美学满意度为100%,明显高于对照,85.37%,差异有统计学意义(χ2=4.623,P=0.032)。结论耳后沟带蒂皮瓣修复难治性耳前瘘管反复感染创面安全、可靠,联合循证护理可有效降低术后并发症发生率,促进切口愈合和皮瓣存活,缩短治疗时间,提高美学效果。

Use of Acellular Fish Skin for Dura Repair in an Ovine Model: A Pilot Study

Recently the use of biologic materials as dura mater repair patches has been increasing. The purpose of this study is to assess the basis for efficacy and safety of using a novel fish derived acellular dermis (Kerecis Omega3 DuraTM). In an ovine model a craniotomy under general anaesthesia was performed. A defect was produced in the dural covering of approximately 1 × 2 cm and closed with an onlay technique, with Kerecis Omega3 Dura. The bone defect was covered with the bony flap and the overlying tissues closed in layers. At 2, 5, 8 and 11 weeks the sheep underwent MRI scanning followed by euthanasia, necropsy and histological assessment. MRI images taken at 2, 5, 8 and 11 weeks showed initially moderate inflammatory response, which diminished over time, and at 11 weeks no evidence of inflammation existed. There was evidence of cerebrospinal fluid leakage at no time point. Necropsy revealed some adhesions at 5 and 8 weeks, in particular at 5 weeks, but at 11 weeks there were no adhesions found. From 2 - 11 weeks, there was evidence of initially an inflammatory reaction followed by neodura formation at the defect site through cellular ingrowth and remodeling of the acellular fish skin. Histology showed a histiocytic foreign body reaction initially that subsided over time. As early as 8 weeks there was evidence of neodura formation and by 11 weeks there was a minimal inflammatory response with an intact neodura formed. In this pilot study the Kerecis Omega3 Dura patches performed in a safe and efficacious manner. This new material needs to be fully assessed and compared with other products that are currently on the market in a larger scale animal study.

局部皮下蒂皮瓣修复非黑色素瘤皮肤癌一期切除术后缺损的效果及安全性

目的探究局部皮下蒂皮瓣对非黑色素瘤皮肤癌一期切除术后缺损的修复效果及安全性。方法选取2016年10月至2023年10月我院非黑色素瘤皮肤癌患者80例。对照组实施传统缝合(n=40),观察组采用皮下蒂皮瓣修复(n=40)。对比两组患者修复疗效、治疗后满意程度及不良反应。结果观察组患者修复疗效总有效率为95.00%大于对照组的75.00%(P<0.05);观察组患者治疗后总满意度为97.50%大于对照组的80.00%(P<0.05);术后两组患者各项创面评分均升高,且观察组更高,均有差异(P<0.05);观察组患者术后总不良反应发生率为7.50%低于对照组的27.50%(P<0.05)。结论对非黑色素瘤皮肤癌,于一期切除术后采用皮下蒂皮瓣修复的治疗方法,不仅可以提升患者术后愈合情况,还可以提高术后满意度,降低不良反应的发生,提高安全性。

面向皮肤大面积损伤修复的原位打印系统工作空间分析

皮肤大面积损伤修复一直是临床上亟待解决的难题。目前,常用的修复方法主要为自体皮肤移植和伤口敷料治疗,但这些方法不能同时满足皮肤大面积修复和定制化治疗的需求。原位皮肤打印技术为皮肤大面积损伤修复提供了新思路,但现有的生物打印设备打印范围小且打印精度低,无法实现皮肤大面积组织的随形打印。为解决上述问题,提出了一种由Stewart并联机器人、直线模组机构、打印头和三维扫描仪等组成的原位皮肤打印系统。其中,Stewart并联机器人的重复定位精度高且累积误差小,其作为打印驱动装置可实现高精度的皮肤原位打印;Stewart并联机器人具有6个自由度,可在三维空间中实时调整打印角度,使得生物墨水能够沿皮肤曲面完整地覆盖在皮肤损伤处,有利于伤口修复。为分析所设计原位皮肤打印系统的可行性,通过数值法计算并联机器人的工作空间,得到了原位皮肤打印系统的工作范围,并通过打印实验进行了验证。实验结果表明,并联机器人可按照指定路径运行,打印头在打印过程中可稳定喷射生物墨水;原位皮肤打印系统的工作范围与并联机器人的工作空间基本吻合,可满足皮肤大面积损伤修复的需求。研究结果为后续的皮肤大面积修复动物实验奠定了理论基础。

乙酰化透明质酸钠改善皮肤的功效

为研究乙酰化透明质酸钠(AcHA)改善皮肤的功效,以AcHA为原料,测试其对皮肤的保湿、修护、紧致、抗氧化性能。结果表明,AcHA累积渗透量为35.9%,性能良好;选用3D表皮皮肤模型,检测表皮皮肤模型的皮肤含水量和天然保湿因子含量的变化评估其保湿功效,观测表皮皮肤模型的组织形态、屏障相关蛋白含量和经表皮失水率的变化以评估其修护功效,研究表明体积分数0.25%的AcHA具有良好的保湿、修护、紧致以及抗氧化功效,为AcHA的进一步开发利用提供了理论基础。

拇指桡背侧皮神经营养血管皮瓣瓦合邻指皮瓣修复拇指末节脱套样软组织缺损

目的:探讨拇指桡背侧皮神经营养血管皮瓣与示指背邻指皮瓣瓦合修复拇指末节脱套样软组织缺损的临床治疗效果。方法:2017年10月-2021年2月,笔者医院对12例拇指末节脱套样软组织缺损患者采用拇指桡背侧皮神经营养血管皮瓣与示指背邻指皮瓣瓦合修复,创面面积2.0 cm×5.5 cm~3.5 cm×7.5 cm,门诊随访皮瓣外观与感觉、手功能及术后并发症情况。结果:拇指桡背侧皮神经营养血管皮瓣切取范围为2.0 cm×2.0 cm~3.5 cm×4.0 cm,示指近节背侧邻指皮瓣切取范围为1.5 cm×2.0 cm~3.0 cm×3.5 cm。术后随访6~18个月,平均9.4个月,11例拇指桡背侧皮神经营养血管皮瓣及10例邻指皮瓣成活良好,伤口甲级愈合。远期随访,10例拇指指腹饱满,外观满意,2例拇指指尖皮瓣萎缩。12例皮瓣供区均愈合良好,无明显瘢痕增生,功能恢复良好。12例拇指恢复良好的对捏及对掌功能,拇指指腹两点感觉辨别试验为7~16 mm,指背两点感觉辨别试验为6~11 mm。评定手功能:优8例,良2例,中2例。结论:拇指桡背侧皮神经营养血管皮瓣与示指背邻指皮瓣瓦合修复拇指末节脱套样软组织缺损,手术操作简便、安全,临床效果好,值得在基层医院推广。

头皮恶性肿瘤切除后创面局部皮瓣转移联合游离皮片移植术修复11例分析

目的探讨头皮恶性肿瘤切除后形成的创面应用局部皮瓣转移联合游离皮片移植术修复的临床效果。方法回顾分析2017年1月至2022年1月在焦作市人民医院皮肤科治疗并完成随访的11例头皮恶性肿瘤患者资料。患者年龄30~91岁,男4例,女7例,基底细胞癌6例,鳞状细胞癌5例,所有患者均采用局部皮瓣转移联合游离皮片移植术修复头皮恶性肿瘤切除后形成的创面。结果11例患者均一期手术完成,所有转移皮瓣和移植皮片均成活,术后均未出现感染,切口甲级愈合。术后随访6~12个月肿瘤无复发,创面修复良好,患者满意度高。结论局部皮瓣转移联合游离皮片移植术是头皮恶性肿瘤术后创面修复的理想方法,具有手术一期完成、转移皮瓣及游离皮片易于成活、肿瘤复发率低、恢复良好的优点。

胶原蛋白肽功能及其作用机制研究进展

胶原蛋白是哺乳动物体内含量最多、分布最广的功能性大分子蛋白质,主要存在于动物的结缔组织中,参与细胞外基质结构的组成,人体很难直接消化吸收。胶原蛋白肽(CP)是胶原蛋白经过酶水解得到的是一种介于氨基酸和蛋白质之间的混合物,因其具有良好的抗氧化活性、生物相容性、低分子量且易被消化吸收等优点,在医疗和食品等领域得到广泛应用。文章主要从CP来源、CP防治骨质疏松症、修复皮肤老化、预防Ⅱ型糖尿病的功能及其作用机制方面进行综述,可以为从事CP研究和开发的专业人员提供新思路。

负压封闭引流联合腓动脉穿支皮瓣修复外踝部毁损伤创面的疗效

目的:探讨负压封闭引流(VSD)联合腓动脉穿支皮瓣修复外踝部毁损伤创面的临床疗效。方法:选取应用VSD联合腓动脉穿支皮瓣修复足踝部毁损伤创面62例为研究对象,所有患者一期清创后行VSD治疗5~7 d,基底清洁后二期行腓动脉穿支皮瓣皮瓣修复。结果:62例患者中有12例患者术后皮瓣中远端出现回流障碍、淤血情况,经48 h内反复行皮瓣内静脉放血后好转。59例腓动脉穿支皮瓣成活良好,皮瓣远端约1~3 cm出现发黑坏死3例,但基底未见肌腱或骨质外露,经换药后治愈2例,再次清创行自体皮片移植后治愈1例。创面愈合时间12~32 d,平均愈合时间16.5 d;随访时间12~15个月,观察皮瓣柔软,与周缘皮肤颜色相近,耐磨性好,外观功能恢复满意,踝关节活动基本正常,未并发溃疡;据美国矫形足踝协会(AOFAS)评估足踝部功能优良率为100%。结论:采用VSD联合腓动脉穿支皮瓣修复足踝部毁损伤创面能获得良好的效果,后期皮瓣质地良好、厚薄适中、耐磨及外观功能恢复良好。

拇指尺背侧皮神经营养血管皮瓣修复拇指C形缺损

目的:探讨拇指尺背侧皮神经营养血管皮瓣修复拇指C形缺损的方法及临床效果。方法:2017年12月-2021年7月,笔者科室采用拇指尺背侧皮神经营养血管皮瓣修复拇指C形缺损患者6例,缺损面积为1.6 cm×2.8 cm~3.5 cm×4.5 cm,缺损范围均大于拇指周径的1/2,不超过3/4,皮瓣切取面积为2.0 cm×3.2 cm~4.0 cm×5.0 cm,供区移植全厚皮片。术后通过电话、微信和门诊随访皮瓣外观、感觉及手功能。结果:术后随访时间为3~26个月,平均9.4个月,所有皮瓣全部存活,供、受区均未发生感染,皮瓣外观良好,两点分辨觉8~22 mm。供区植皮均成活良好,2例供区愈合后遗留线性瘢痕。手指功能:优4例,良1例,可1例。结论:拇指尺背侧皮神经营养血管皮瓣修复拇指C形缺损,手术操作简单,成活率高,不损伤主要血管,术后疗效满意。

SDF-1/CXCR4轴在皮肤及周围神经损伤修复中的研究进展

皮肤组织遭受损伤时,其损伤修复对于恢复皮肤的稳态非常重要,趋化因子SDF-1在此过程中起着非常关键的作用。本文对SDF-1/CXCR4轴在创面愈合、损伤处神经修复作用、创伤后瘢痕形成以及其对损伤部位神经疼痛的影响进行综述,为开发更有效的皮肤及周围神经损伤的治疗方法和策略提供指导。

局部皮瓣联合异体巩膜移植修复眼睑及其周围皮肤缺损疗效观察

目的:观察局部皮瓣联合异体巩膜移植修复眼睑及其周围皮肤缺损的临床效果。方法:选取2019年1月-2021年1月笔者医院收治的90例眼睑恶性肿瘤切除术后眼睑及周围皮肤缺损患者,采用随机数字表法分为观察组和对照组,各45例,对照组采用异体巩膜移植修复,观察组在此基础上联合局部皮瓣修复,比较两组修复效果,修复前后睑裂长度、眼睑高度差值以及修复后6个月瘢痕宽度、瘢痕颜色、弹性、平整度、眼睑形态恢复程度、外观满意度评分,以及并发症发生情况。结果:观察组修复总有效率高于对照组(P<0.05);两组修复后睑裂长度、眼睑高度均较修复前降低(P<0.05),且观察组低于对照组(P<0.05);观察组修复后6个月瘢痕宽度小于对照组,瘢痕颜色、弹性、平整度、眼睑形态恢复程度、外观满意度评分均高于对照组(P<0.05);观察组术后上睑下垂、睑缘切痕等并发症总发生率低于对照组(P<0.05)。结论:局部皮瓣联合异体巩膜移植修复眼睑及其周围皮肤缺损疗效显著且并发症少,术后眼睑形态及美学效果良好。

清热生肌膏介导NLRP3对放射性皮炎大鼠MPO、Hyp表达的影响

目的 清热生肌膏介导NLRP3炎性小体对放射性皮炎大鼠皮肤损伤修复及MPO、Hyp表达水平的影响。方法 选取SD健康大鼠90只,80只随机分为正常组、模型组(放射性皮炎大鼠模型)、治疗组(模型+清热生肌膏)、对照组(模型+复方茶多酚软膏),每组各20只。另设抑制剂组(NLRP3抑制剂)10只。通过创面愈合率衡量创面愈合情况。透射电镜观察创面细胞超微结构。HE染色观察病理学变化。分光光度比色法测定MPO活性。免疫印迹检测NLRP3、Caspase-1蛋白表达。结果 各组在治疗后第1 d时的创面愈合无差异(P>0.05)。模型组大鼠在治疗3~21 d时的创面愈合均低于治疗组及对照组(P<0.05)。治疗组与对照组大鼠治疗治疗3~21 d各时间点创面愈合比较无差异(P>0.05)。与正常组相比,模型组大鼠MPO表达升高、Hyp表达降低(P<0.05)。与模型组相比,治疗组大鼠MPO表达降低、Hyp表达升高(P<0.05)。治疗组与对照组MPO及Hyp表达对比无差异(P>0.05)。与正常组相比,模型组大鼠NLRP3、Caspase-1表达升高(P<0.05)。与模型组相比,治疗组大鼠NLRP3、Caspase-1蛋白表达降低(P<0.05)。治疗组与抑制剂组大鼠NLRP3、Caspase-1蛋白表达对比无差异(P>0.05)。结论 清热生肌膏可抑制MPO水平并促进Hyp表达,对放射性皮炎大鼠皮肤损伤修复有积极促进作用,其作用机制可能与介导NLRP3炎性小体有关。