A kind of Levextrel resin separation process was developed for separation ofindium (III), gallium (III), and zinc (II) from aqueous sulfate solution with Levextrel resincontaining di(2-ethylhexyl) phosphoric acid (CL-...A kind of Levextrel resin separation process was developed for separation ofindium (III), gallium (III), and zinc (II) from aqueous sulfate solution with Levextrel resincontaining di(2-ethylhexyl) phosphoric acid (CL-P 204). The aim of the research is to collectpreliminary results for a pilot-scale production. Properties of adsorbing indium (III), gallium(III), and zinc (II) from sulfate solution with the Levextrel resin were first studied by batchoperation and column operation. The optimum pH, adsorption capacities and concentrations ofstripping agents for indium (III), gallium (III) were tested. The separation order of indium (III),gallium (III), and zinc (II) from sulfate solution with CL-P 204 Levextrel resin was found thatindium (III) could be first separated by adsorbing at the acidity of 1.0 mol/L whereas gallium (III)and zinc (II) could not, and they were adsorbed together by adsorbing at pH =2.8, then separatedfrom each other by stripping with 0.1 and 0.5 mol/L hydrochloric acid, respectively. The recoveriesof three metal ions were all higher than 99 percent. The cyclic properties of this resin are well.展开更多
Glycyrrhetic acid 3-O-mono-β-D-glucuronide （GAMG）, the major functional ingredient in licorice, has widespread applications in food, pharmacy and cosmetics industry. The production of GAMG through Penicillium purpu...Glycyrrhetic acid 3-O-mono-β-D-glucuronide （GAMG）, the major functional ingredient in licorice, has widespread applications in food, pharmacy and cosmetics industry. The production of GAMG through Penicillium purpurogenum Li-3 cultivation was for the first time performed through both batch and fed-batch processes in bioreactors. In batch process, under optimal conditions （pH 5.0, temperature 32℃, agitation speed 100 r. rain 1）, 3.55 g. L^-1 GAMG was obtained in a 2.5 L fermentor. To further enhance GAMG production, a fine fed-batch process was developed by using pH and DO as feedback parameters. Starting from 48 h, 100 m190 g-L 1 substrate Glycyrrhizin （GL） was fed each time when pH increased to above 5.0 and DO was increased to above 80%. This strategy can significantly enhance GAMG production： the achieved GL conversion was 95.34% with GAMG yield of 95.15%, and GAMG concentration was 16.62 g. L^-1 which was 5 times higher than that of batch. Then, a two-step separation strat- egy was established to separate GAMG from fermentation broth by crude extraction of 15 ml column packed with D10I resin followed by fine purification with preparative C18 chromatography. The obtained GAMG purity was 95.79%. This study provides a new insight into the industrial bioprocess of high-level GAMG production.展开更多
文摘A kind of Levextrel resin separation process was developed for separation ofindium (III), gallium (III), and zinc (II) from aqueous sulfate solution with Levextrel resincontaining di(2-ethylhexyl) phosphoric acid (CL-P 204). The aim of the research is to collectpreliminary results for a pilot-scale production. Properties of adsorbing indium (III), gallium(III), and zinc (II) from sulfate solution with the Levextrel resin were first studied by batchoperation and column operation. The optimum pH, adsorption capacities and concentrations ofstripping agents for indium (III), gallium (III) were tested. The separation order of indium (III),gallium (III), and zinc (II) from sulfate solution with CL-P 204 Levextrel resin was found thatindium (III) could be first separated by adsorbing at the acidity of 1.0 mol/L whereas gallium (III)and zinc (II) could not, and they were adsorbed together by adsorbing at pH =2.8, then separatedfrom each other by stripping with 0.1 and 0.5 mol/L hydrochloric acid, respectively. The recoveriesof three metal ions were all higher than 99 percent. The cyclic properties of this resin are well.
基金Supported by the National Natural Science Foundation of China(21176028 and21506011)the National Science Fund for Distinguished Young Scholars of China(21425624)Doctoral Fund of Ministry of Education of China(20121101110050)
文摘Glycyrrhetic acid 3-O-mono-β-D-glucuronide （GAMG）, the major functional ingredient in licorice, has widespread applications in food, pharmacy and cosmetics industry. The production of GAMG through Penicillium purpurogenum Li-3 cultivation was for the first time performed through both batch and fed-batch processes in bioreactors. In batch process, under optimal conditions （pH 5.0, temperature 32℃, agitation speed 100 r. rain 1）, 3.55 g. L^-1 GAMG was obtained in a 2.5 L fermentor. To further enhance GAMG production, a fine fed-batch process was developed by using pH and DO as feedback parameters. Starting from 48 h, 100 m190 g-L 1 substrate Glycyrrhizin （GL） was fed each time when pH increased to above 5.0 and DO was increased to above 80%. This strategy can significantly enhance GAMG production： the achieved GL conversion was 95.34% with GAMG yield of 95.15%, and GAMG concentration was 16.62 g. L^-1 which was 5 times higher than that of batch. Then, a two-step separation strat- egy was established to separate GAMG from fermentation broth by crude extraction of 15 ml column packed with D10I resin followed by fine purification with preparative C18 chromatography. The obtained GAMG purity was 95.79%. This study provides a new insight into the industrial bioprocess of high-level GAMG production.
