BACKGROUND Breast milk is the primary source of nutrition for newborns.Hospitalized babies frequently need nutritional support from Human Milk Banks.As bacterial species of the genus Enterococcus are part of the micro...BACKGROUND Breast milk is the primary source of nutrition for newborns.Hospitalized babies frequently need nutritional support from Human Milk Banks.As bacterial species of the genus Enterococcus are part of the microbiota of healthy donors,they may contaminate samples of pumped breast milk.AIM To identify and characterize the bacterial virulence and resistance in samples isolated from the nipple-areolar region,hands,and breast milk aliquots from donors at the Human Milk Bank of Municipal Hospital EsauMatos in the city of Vitória da Conquista,Bahia State,Brazil.METHODS The personal hygiene and sanitation of donors were analyzed with the aim of identifying possible reasons for contamination of pumped milk.Cutaneous samples as well as aliquots of unpasteurized and pasteurized milk from 30 participants were obtained.Each Enterococcus spp.isolate underwent a disk diffusion susceptibility test and molecular biology techniques to determine resistance and virulence genes.RESULTS Enterococcus spp.were identified in 30%of donors(n=9),and 11 specimens were isolated.Resistance to tetracycline was highly prevalent,being detectable in 63%of the isolates(n=7)and followed by intermediate sensitivity to ciprofloxacin,observed in 27%of the specimens(n=3).The efaA gene was found in 63%(n=7)of the isolates,while the ace gene was detected in 27%(n=3).CONCLUSION This study illustrates the importance of microbiological monitoring by Human Milk Banks and the need for alternatives to prevent the presence of Enterococcus spp.in hospital settings.展开更多
目的分析我院鲍曼不动杆菌临床分布特征及耐药性,为合理使用抗菌药物和预防控制院内感染提供依据。方法收集2016年1月~2020年12月鲍曼不动杆菌分离株,根据临床和实验室标准协会(clinical and laboratory standards institute,CLSI)标准...目的分析我院鲍曼不动杆菌临床分布特征及耐药性,为合理使用抗菌药物和预防控制院内感染提供依据。方法收集2016年1月~2020年12月鲍曼不动杆菌分离株,根据临床和实验室标准协会(clinical and laboratory standards institute,CLSI)标准判读结果,用WHONET 5.6软件分析药敏结果数据。结果共分离出鲍曼不动杆菌1731株。主要分离自下呼吸道标本,共922株(53.3%),其他依次为引流液、尿液、分泌物、全血和胆汁等标本。科室分布以肝胆外科、重症医学科、呼吸内科和心血管外科为主,占55.8%。年龄分布主要为中老年患者,男性明显多于女性(P<0.05)。该菌对替加环素和米诺环素具有较低的耐药性,平均耐药性分别为5.6%、16.3%;对阿米卡星、妥布霉素、左氧氟沙星、头孢哌酮/舒巴坦和复方新诺明的平均耐药率为50%~57%;对头孢菌素类、碳青霉烯类、多西环素和环丙沙星的平均耐药率高于68.0%。结论近5年我院鲍曼不动杆菌感染主要感染部位为呼吸系统;年龄越大,感染的概率越大。该菌具有极强的耐药性,应加强耐药性监测,并对重点科室做好医院感染监测。展开更多
文摘BACKGROUND Breast milk is the primary source of nutrition for newborns.Hospitalized babies frequently need nutritional support from Human Milk Banks.As bacterial species of the genus Enterococcus are part of the microbiota of healthy donors,they may contaminate samples of pumped breast milk.AIM To identify and characterize the bacterial virulence and resistance in samples isolated from the nipple-areolar region,hands,and breast milk aliquots from donors at the Human Milk Bank of Municipal Hospital EsauMatos in the city of Vitória da Conquista,Bahia State,Brazil.METHODS The personal hygiene and sanitation of donors were analyzed with the aim of identifying possible reasons for contamination of pumped milk.Cutaneous samples as well as aliquots of unpasteurized and pasteurized milk from 30 participants were obtained.Each Enterococcus spp.isolate underwent a disk diffusion susceptibility test and molecular biology techniques to determine resistance and virulence genes.RESULTS Enterococcus spp.were identified in 30%of donors(n=9),and 11 specimens were isolated.Resistance to tetracycline was highly prevalent,being detectable in 63%of the isolates(n=7)and followed by intermediate sensitivity to ciprofloxacin,observed in 27%of the specimens(n=3).The efaA gene was found in 63%(n=7)of the isolates,while the ace gene was detected in 27%(n=3).CONCLUSION This study illustrates the importance of microbiological monitoring by Human Milk Banks and the need for alternatives to prevent the presence of Enterococcus spp.in hospital settings.
