An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the rest...An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the restriction enzyme and was linked with pTA plasmid to construct pTA TUB2 plasmid.The plasmid was transformed into Chaetomium spp.by PEG method and the transformation rate was 27/(2×10 5) and it is nine times higher than that of pRB 129.The transformants can grow on the PDA containing 1 000 μg·mL -1 carbendazim,which is 1 000 times higher than the original Chaetomium spp.The resistance was stable after 10 times transfer on non selective medium.展开更多
基金Heilongjiang Province Natural Science fund( Grant No.C0 2 0 3 )
文摘An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TUB2 gene.The gene was digested by the restriction enzyme and was linked with pTA plasmid to construct pTA TUB2 plasmid.The plasmid was transformed into Chaetomium spp.by PEG method and the transformation rate was 27/(2×10 5) and it is nine times higher than that of pRB 129.The transformants can grow on the PDA containing 1 000 μg·mL -1 carbendazim,which is 1 000 times higher than the original Chaetomium spp.The resistance was stable after 10 times transfer on non selective medium.
